Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 837,132 C→T 100% intergenic (‑145/+120) fiu ← / ← mcbA catecholate siderophore receptor Fiu/colanic acid mucoidy stimulation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273837,1320CT95.2% 781.8 / 23.3 249intergenic (‑145/+120)fiu/mcbAcatecholate siderophore receptor Fiu/colanic acid mucoidy stimulation protein
Reads supporting (aligned to +/- strand):  ref base C (6/6);  new base T (141/96);  total (147/102)
Fisher's exact test for biased strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 251 total aligned reads displayed.

TTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCAGTACC  >  CP009273/836987‑837275
                                                                                                                                                 |                                                                                                                                               
ttttGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTa                                                                                                                                               >  1:2606132/1‑148 (MQ=255)
 tttGCAGGTGACTTTTTCTTATCTGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTAt                                                                                                                                              >  2:3213929/1‑148 (MQ=255)
   tGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCa                                                                                                                                            >  2:164147/1‑148 (MQ=255)
       ggTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCa                                                                                                                                        >  2:2570773/1‑148 (MQ=255)
        gTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGATAAATCCTCTCTGAGGATGCGATGCCACTGTTAATCCCGAGATAGTGCTATAGAAAGTGATAATAATTTTGATAAGCATTATCACTAGGAGCGATTTTTTATCACACAt                                                                                                                                       >  2:348622/1‑148 (MQ=255)
        gTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCAt                                                                                                                                       <  1:1669241/148‑1 (MQ=255)
        gTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATATTTTATCACCCAt                                                                                                                                       >  2:2861336/1‑148 (MQ=255)
           aCTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTgc                                                                                                                                    >  1:3246071/1‑148 (MQ=255)
            cTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTgcg                                                                                                                                   >  2:3518797/1‑148 (MQ=255)
                  cTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGaaaa                                                                                                                             <  1:2505521/148‑1 (MQ=255)
                    tataTGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAg                                                                                                                           >  1:617917/1‑148 (MQ=255)
                     atatGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGa                                                                                                                          >  2:864502/1‑148 (MQ=255)
                     atatGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGa                                                                                                                          >  2:2644197/1‑148 (MQ=255)
                     atatGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGa                                                                                                                          >  1:2280795/1‑148 (MQ=255)
                     atatGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGa                                                                                                                          >  1:1970166/1‑148 (MQ=255)
                     atatGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGAGCGAAAAAGa                                                                                                                          >  2:3200368/1‑148 (MQ=255)
                     atatGTTGAAATTTTCACTGCCACGAGAAAGCCACTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGa                                                                                                                          >  1:1736779/1‑148 (MQ=255)
                        tGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAAt                                                                                                                       <  1:2786935/148‑1 (MQ=255)
                          ttGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATAc                                                                                                                     >  1:2999622/1‑148 (MQ=255)
                           tGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACa                                                                                                                    <  1:1204265/148‑1 (MQ=255)
                              aaTTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACattt                                                                                                                 <  2:3079678/148‑1 (MQ=255)
                               aTTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACattta                                                                                                                >  1:1519695/1‑148 (MQ=255)
                                ttttCACTGCCACGAGAAAGCCTCTCTTCGGATGCGATGCCACGGTTAATCCCGTGATAGAGCTCGTGAAACTTATAATAATTTTGATAAGCAATATCACATGTAGCGATATTTTATCAACAATTGCGCGAAAAAGAAATAAatttat                                                                                                               >  1:2082386/1‑148 (MQ=255)
                                ttttCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACatttat                                                                                                               <  1:2906938/148‑1 (MQ=255)
                                ttttCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACatttat                                                                                                               >  2:2768714/1‑148 (MQ=255)
                                ttttCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACatttat                                                                                                               >  2:67530/1‑148 (MQ=255)
                                 tttCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACatttatt                                                                                                              >  1:3328083/1‑148 (MQ=255)
                                 tttCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACatttatt                                                                                                              >  1:2187035/1‑148 (MQ=255)
                                 tttCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACatttatt                                                                                                              >  1:1486187/1‑148 (MQ=255)
                                   tCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACAtttattta                                                                                                            >  2:497374/1‑148 (MQ=255)
                                     aCTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACa                                                                                                          >  2:1307184/1‑148 (MQ=255)
                                          cACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATGTACATTGAg                                                                                                     >  1:2241087/1‑148 (MQ=255)
                                           aCGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGt                                                                                                    >  2:1147238/1‑148 (MQ=255)
                                           aCGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGt                                                                                                    >  2:96326/1‑148 (MQ=255)
                                            cGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGtt                                                                                                   <  2:1421233/148‑1 (MQ=255)
                                            cGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGtt                                                                                                   >  1:3267569/1‑148 (MQ=255)
                                             gagaAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTc                                                                                                  >  2:2057903/1‑148 (MQ=255)
                                             gagaAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTc                                                                                                  <  1:1010268/148‑1 (MQ=255)
                                                aaaGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCata                                                                                               <  2:2820471/148‑1 (MQ=255)
                                                aaaGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCata                                                                                               >  1:1329633/1‑148 (MQ=255)
                                                 aaGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCatat                                                                                              <  1:1049278/148‑1 (MQ=255)
                                                   gCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATAttt                                                                                            >  1:1628750/1‑148 (MQ=255)
                                                   gCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATAttt                                                                                            >  1:779091/1‑148 (MQ=255)
                                                     ctctctGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGa                                                                                          <  1:967638/148‑1 (MQ=255)
                                                         ctGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGtt                                                                                      <  2:2415111/148‑1 (MQ=255)
                                                          tGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGttt                                                                                     <  2:3318054/148‑1 (MQ=255)
                                                           gCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGtttt                                                                                    >  1:1760203/1‑148 (MQ=255)
                                                           gCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGtttt                                                                                    >  2:156551/1‑148 (MQ=255)
                                                            cGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGttttt                                                                                   <  1:2137388/148‑1 (MQ=255)
                                                               atgcgatgcCACTGTTAATCCCGTGATAGTGCTCGTGAAGGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGa                                                                                <  2:1061231/148‑1 (MQ=255)
                                                               atgcgatgcCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGa                                                                                >  2:1942015/1‑148 (MQ=255)
                                                               atgcgatgcCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGa                                                                                <  2:1566925/148‑1 (MQ=255)
                                                                    atgcCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACa                                                                           <  2:1523500/148‑1 (MQ=255)
                                                                        cACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAAc                                                                       <  2:2144650/148‑1 (MQ=255)
                                                                         aCTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACa                                                                      >  1:3475630/1‑148 (MQ=255)
                                                                           tGTTAATCCAGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATAGATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGa                                                                    <  1:1545777/148‑1 (MQ=255)
                                                                            gTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGAc                                                                   <  1:471024/148‑1 (MQ=255)
                                                                               aaTCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGaa                                                                <  2:3104244/148‑1 (MQ=255)
                                                                                 tCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGaaaa                                                              >  2:1707571/1‑148 (MQ=255)
                                                                                    cGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTg                                                           >  1:1815635/1‑148 (MQ=255)
                                                                                     gTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGc                                                          <  2:802287/148‑1 (MQ=255)
                                                                                     gTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGc                                                          >  1:3271818/1‑148 (MQ=255)
                                                                                      tGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCa                                                         <  1:308723/148‑1 (MQ=255)
                                                                                        aTAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATc                                                       >  2:2945990/1‑148 (MQ=255)
                                                                                         tAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATcc                                                      <  2:250419/148‑1 (MQ=255)
                                                                                          aGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAATCTGCATCCa                                                     >  2:1509217/1‑148 (MQ=255)
                                                                                            tGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCaga                                                   <  1:1159834/148‑1 (MQ=255)
                                                                                                    aaaGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATg                                           <  1:2924530/148‑1 (MQ=255)
                                                                                                     aaGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGc                                          >  2:1620538/1‑148 (MQ=255)
                                                                                                      aGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCa                                         >  2:1220608/1‑148 (MQ=255)
                                                                                                      aGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCa                                         >  2:1724429/1‑148 (MQ=255)
                                                                                                      aGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCa                                         >  2:2160717/1‑148 (MQ=255)
                                                                                                       gTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAg                                        <  1:1659029/148‑1 (MQ=255)
                                                                                                       gTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAg                                        <  1:2939909/148‑1 (MQ=255)
                                                                                                       gTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAg                                        <  1:2316578/148‑1 (MQ=255)
                                                                                                        tGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGt                                       >  1:2516735/1‑148 (MQ=255)
                                                                                                         gATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGtt                                      >  1:2262346/1‑148 (MQ=255)
                                                                                                              taatTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGtt                                 <  1:3414210/148‑1 (MQ=255)
                                                                                                               aatTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTtg                                >  1:2685774/1‑148 (MQ=255)
                                                                                                                 ttttGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCCGTTACGTtggt                              >  2:221164/1‑148 (MQ=255)
                                                                                                                 ttttGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTtggt                              >  1:1377968/1‑148 (MQ=255)
                                                                                                                  tttGATAAGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTtggtg                             <  2:2999622/148‑1 (MQ=255)
                                                                                                                   ttGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTtggtgg                            <  1:2763055/148‑1 (MQ=255)
                                                                                                                      aTAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGt                         <  2:1455217/148‑1 (MQ=255)
                                                                                                                       tAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGtt                        >  1:1948660/1‑148 (MQ=255)
                                                                                                                        aaGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTa                       <  2:1014135/148‑1 (MQ=255)
                                                                                                                        aaGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTa                       <  2:2060445/148‑1 (MQ=255)
                                                                                                                         aGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTAt                      >  2:2602219/1‑148 (MQ=255)
                                                                                                                          gCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTAtt                     >  2:187876/1‑148 (MQ=255)
                                                                                                                           cATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTAttt                    >  1:2360612/1‑148 (MQ=255)
                                                                                                                            aTTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTg                   <  2:1737910/148‑1 (MQ=255)
                                                                                                                               aTCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAg                >  2:2193064/1‑148 (MQ=255)
                                                                                                                                tCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGa               <  1:722945/148‑1 (MQ=255)
                                                                                                                                 cACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGAt              >  1:362470/1‑148 (MQ=255)
                                                                                                                                     tGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGtt          >  2:1749290/1‑148 (MQ=255)
                                                                                                                                       gAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGc        >  1:553138/1‑148 (MQ=255)
                                                                                                                                       gAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGc        >  1:1872559/1‑148 (MQ=255)
                                                                                                                                       gAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGc        >  1:3312742/1‑148 (MQ=255)
                                                                                                                                            aTTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCAGTAc   <  1:497434/148‑1 (MQ=255)
                                                                                                                                            aTTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCAGTAc   <  2:328515/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCAGTACC  >  CP009273/836987‑837275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCAGTACCG  >  CP009273/836985‑837276
                                                                                                                                                   |                                                                                                                                                
CATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTA                                                                                                                                                >  SRR19973067.2623067/1‑150 (MQ=60)
          GTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTG                                                                                                                                      <  SRR19973067.1680755/150‑1 (MQ=60)
          GTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGACACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTG                                                                                                                                      <  SRR19973067.1680746/150‑1 (MQ=60)
           TGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGC                                                                                                                                     >  SRR19973067.3266400/1‑150 (MQ=60)
           TGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGC                                                                                                                                     >  SRR19973067.3266430/1‑150 (MQ=60)
            GACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCG                                                                                                                                    <  SRR19973067.1786345/150‑1 (MQ=60)
              CTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCG                                                                                                                                  >  SRR19973067.840132/1‑150 (MQ=60)
                    CTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAG                                                                                                                            <  SRR19973067.2521964/150‑1 (MQ=60)
                    CTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAG                                                                                                                            <  SRR19973067.1903360/150‑1 (MQ=60)
                    CTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAG                                                                                                                            >  SRR19973067.621968/1‑150 (MQ=60)
                     TTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGA                                                                                                                           >  SRR19973067.2296050/1‑150 (MQ=60)
                     TTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGA                                                                                                                           >  SRR19973067.2295877/1‑150 (MQ=60)
                     TTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGA                                                                                                                           >  SRR19973067.1983539/1‑150 (MQ=60)
                     TTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGA                                                                                                                           >  SRR19973067.1983527/1‑150 (MQ=60)
                     TTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGA                                                                                                                           >  SRR19973067.1983495/1‑150 (MQ=60)
                     TTATATGTTGAAATTTTCACTGCCACGAGAAAGCCACTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGA                                                                                                                           >  SRR19973067.1748660/1‑150 (MQ=60)
                       ATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAA                                                                                                                         <  SRR19973067.2978018/150‑1 (MQ=60)
                          TGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATAC                                                                                                                      <  SRR19973067.2804917/150‑1 (MQ=60)
                          TGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATAC                                                                                                                      >  SRR19973067.3018659/1‑150 (MQ=60)
                             TGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATT                                                                                                                   <  SRR19973067.1211092/150‑1 (MQ=60)
                             TGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATT                                                                                                                   <  SRR19973067.1212147/150‑1 (MQ=60)
                              GAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTT                                                                                                                  >  SRR19973067.112107/1‑150 (MQ=60)
                               AAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTA                                                                                                                 >  SRR19973067.1529859/1‑150 (MQ=60)
                                AATTTTCACTGCCACGAGAAAGCCTCTCTTCGGATGCGATGCCACGGTTAATCCCGTGATAGAGCTCGTGAAACTTATAATAATTTTGATAAGCAATATCACATGTAGCGATATTTTATCAACAATTGCGCGAAAAAGAAATAAATTTAT                                                                                                                >  SRR19973067.2096182/1‑150 (MQ=60)
                                AATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTAT                                                                                                                >  SRR19973067.333404/1‑150 (MQ=60)
                                 ATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATT                                                                                                               >  SRR19973067.1496033/1‑150 (MQ=60)
                                 ATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATT                                                                                                               >  SRR19973067.1495752/1‑150 (MQ=60)
                                 ATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATT                                                                                                               >  SRR19973067.3349136/1‑150 (MQ=60)
                                 ATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATT                                                                                                               >  SRR19973067.1496038/1‑150 (MQ=60)
                                 ATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATT                                                                                                               >  SRR19973067.2048988/1‑150 (MQ=60)
                                 ATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATT                                                                                                               >  SRR19973067.2201642/1‑150 (MQ=60)
                                  TTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTT                                                                                                              <  SRR19973067.1650760/150‑1 (MQ=60)
                                  TTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTT                                                                                                              >  SRR19973067.956897/1‑150 (MQ=60)
                                  TTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTT                                                                                                              <  SRR19973067.2925461/150‑1 (MQ=60)
                                  TTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACACATTGCGCGAAAAAGAAATACATTTATTT                                                                                                              >  SRR19973067.3370386/1‑150 (MQ=60)
                                      CACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACAT                                                                                                          <  SRR19973067.3491111/150‑1 (MQ=60)
                                      CACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACAT                                                                                                          >  SRR19973067.3932/1‑150 (MQ=60)
                                       ACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATT                                                                                                         <  SRR19973067.3285465/150‑1 (MQ=60)
                                          GCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAG                                                                                                      <  SRR19973067.3543575/150‑1 (MQ=60)
                                          GCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAG                                                                                                      <  SRR19973067.2104956/150‑1 (MQ=60)
                                          GCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATGTACATTGAG                                                                                                      >  SRR19973067.2255987/1‑150 (MQ=60)
                                            CACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTT                                                                                                    >  SRR19973067.3287957/1‑150 (MQ=60)
                                            CACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTT                                                                                                    >  SRR19973067.3288362/1‑150 (MQ=60)
                                             ACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTC                                                                                                   >  SRR19973067.2170530/1‑150 (MQ=60)
                                             ACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTC                                                                                                   >  SRR19973067.2170470/1‑150 (MQ=60)
                                               GAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCAT                                                                                                 <  SRR19973067.1783870/150‑1 (MQ=60)
                                               GAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCAT                                                                                                 <  SRR19973067.1017208/150‑1 (MQ=60)
                                               GAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCAT                                                                                                 <  SRR19973067.2804649/150‑1 (MQ=60)
                                               GAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCAT                                                                                                 <  SRR19973067.605479/150‑1 (MQ=60)
                                                AGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATA                                                                                                >  SRR19973067.1338710/1‑150 (MQ=60)
                                                  AAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATT                                                                                              >  SRR19973067.703268/1‑150 (MQ=60)
                                                   AAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTT                                                                                             >  SRR19973067.784484/1‑150 (MQ=60)
                                                   AAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTT                                                                                             >  SRR19973067.1639947/1‑150 (MQ=60)
                                                   AAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTT                                                                                             <  SRR19973067.1056478/150‑1 (MQ=60)
                                                   AAGCCTCTCTGAGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAATTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTT                                                                                             <  SRR19973067.3527056/150‑1 (MQ=60)
                                                      CCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGAT                                                                                          <  SRR19973067.1644475/150‑1 (MQ=60)
                                                       CTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATG                                                                                         <  SRR19973067.2930973/150‑1 (MQ=60)
                                                       CTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATG                                                                                         <  SRR19973067.974270/150‑1 (MQ=60)
                                                          TCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTT                                                                                      <  SRR19973067.255500/150‑1 (MQ=60)
                                                           CTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTT                                                                                     <  SRR19973067.1045779/150‑1 (MQ=60)
                                                           CTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTT                                                                                     >  SRR19973067.1772187/1‑150 (MQ=60)
                                                              CGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTG                                                                                  <  SRR19973067.3199399/150‑1 (MQ=60)
                                                              CGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTG                                                                                  <  SRR19973067.2151670/150‑1 (MQ=60)
                                                                         CCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACA                                                                       >  SRR19973067.3497702/1‑150 (MQ=60)
                                                                            CTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGAC                                                                    <  SRR19973067.17651/150‑1 (MQ=60)
                                                                             TGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACG                                                                   <  SRR19973067.1556278/150‑1 (MQ=60)
                                                                             TGTTAATCCAGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATAGATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACG                                                                   <  SRR19973067.1556280/150‑1 (MQ=60)
                                                                              GTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGA                                                                  <  SRR19973067.475232/150‑1 (MQ=60)
                                                                              GTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGA                                                                  <  SRR19973067.474282/150‑1 (MQ=60)
                                                                               TTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAA                                                                 <  SRR19973067.1316102/150‑1 (MQ=60)
                                                                                  ATCCCGTGATAGTGCTCGTGAAAGTGTTAATAATTTTGATAAGAATTATCACTTGGAGCTATTTTTTATCACCCTTTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAAC                                                              <  SRR19973067.2374093/150‑1 (MQ=60)
                                                                                   TCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCAGATTTGATGTTTTTTGATTACAAAACAGACGAAAACT                                                             >  SRR19973067.3433936/1‑150 (MQ=60)
                                                                                    CCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTG                                                            >  SRR19973067.169525/1‑150 (MQ=60)
                                                                                    CCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTG                                                            >  SRR19973067.1827926/1‑150 (MQ=60)
                                                                                     CCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGC                                                           >  SRR19973067.3292263/1‑150 (MQ=60)
                                                                                       GTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCAT                                                         >  SRR19973067.1707628/1‑150 (MQ=60)
                                                                                       GTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCAGATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCAT                                                         >  SRR19973067.2529167/1‑150 (MQ=60)
                                                                                        TGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATC                                                        <  SRR19973067.1840523/150‑1 (MQ=60)
                                                                                        TGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATC                                                        <  SRR19973067.310733/150‑1 (MQ=60)
                                                                                          ATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCA                                                      >  SRR19973067.992453/1‑150 (MQ=60)
                                                                                          ATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCAA                                                      >  SRR19973067.2779919/1‑150 (MQ=60)
                                                                                              TGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGG                                                  <  SRR19973067.1167559/150‑1 (MQ=60)
                                                                                              TGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGG                                                  >  SRR19973067.2619091/1‑150 (MQ=60)
                                                                                                CTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCG                                                >  SRR19973067.1461443/1‑150 (MQ=60)
                                                                                                   GTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAAT                                             <  SRR19973067.561122/150‑1 (MQ=60)
                                                                                                      AAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCA                                          <  SRR19973067.2943115/150‑1 (MQ=60)
                                                                                                      AAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCA                                          <  SRR19973067.2943263/150‑1 (MQ=60)
                                                                                                        AGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGT                                        >  SRR19973067.2533236/1‑150 (MQ=60)
                                                                                                        AGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGT                                        >  SRR19973067.740859/1‑150 (MQ=60)
                                                                                                         GTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTT                                       >  SRR19973067.2277351/1‑150 (MQ=60)
                                                                                                         GTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTT                                       >  SRR19973067.2277371/1‑150 (MQ=60)
                                                                                                         GTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTT                                       >  SRR19973067.2277060/1‑150 (MQ=60)
                                                                                                         GTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTT                                       <  SRR19973067.1670473/150‑1 (MQ=60)
                                                                                                         GTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTT                                       <  SRR19973067.2331816/150‑1 (MQ=60)
                                                                                                         GTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTT                                       <  SRR19973067.532421/150‑1 (MQ=60)
                                                                                                         GTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTT                                       <  SRR19973067.2958552/150‑1 (MQ=60)
                                                                                                         GTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTT                                       <  SRR19973067.2958494/150‑1 (MQ=60)
                                                                                                          TGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTA                                      >  SRR19973067.1104145/1‑150 (MQ=60)
                                                                                                            ATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACG                                    >  SRR19973067.2417160/1‑150 (MQ=60)
                                                                                                               ATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTG                                 >  SRR19973067.2703004/1‑150 (MQ=60)
                                                                                                               ATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTG                                 >  SRR19973067.2666753/1‑150 (MQ=60)
                                                                                                               ATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTG                                 >  SRR19973067.284016/1‑150 (MQ=60)
                                                                                                               ATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTG                                 >  SRR19973067.283988/1‑150 (MQ=60)
                                                                                                                TAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGG                                >  SRR19973067.2770966/1‑150 (MQ=60)
                                                                                                                TAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGG                                <  SRR19973067.3435946/150‑1 (MQ=60)
                                                                                                                 AATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGT                               >  SRR19973067.1386658/1‑150 (MQ=60)
                                                                                                                 AATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGT                               >  SRR19973067.1387366/1‑150 (MQ=60)
                                                                                                                 AATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGGTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGT                               >  SRR19973067.3305943/1‑150 (MQ=60)
                                                                                                                     TTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGG                           <  SRR19973067.2780909/150‑1 (MQ=60)
                                                                                                                       GATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTT                         >  SRR19973067.1961936/1‑150 (MQ=60)
                                                                                                                           AGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTT                     >  SRR19973067.1324420/1‑150 (MQ=60)
                                                                                                                           AGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTT                     >  SRR19973067.2376023/1‑150 (MQ=60)
                                                                                                                           AGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTT                     >  SRR19973067.2375998/1‑150 (MQ=60)
                                                                                                                              ATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTA                  >  SRR19973067.2264885/1‑150 (MQ=60)
                                                                                                                              ATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTA                  >  SRR19973067.155436/1‑150 (MQ=60)
                                                                                                                                 ATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGAT               >  SRR19973067.364986/1‑150 (MQ=60)
                                                                                                                                  TCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATG              <  SRR19973067.727969/150‑1 (MQ=60)
                                                                                                                                   CACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGG             <  SRR19973067.22296/150‑1 (MQ=60)
                                                                                                                                     CTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTT           >  SRR19973067.747432/1‑150 (MQ=60)
                                                                                                                                       TGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACTAAAACTGCATCCAGAGGCGACTTCAGTTACGTTGGTGGGGTTATGTGTAGATGGTTGC         >  SRR19973067.3088117/1‑150 (MQ=60)
                                                                                                                                       TGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGACTGCAGTTACGTTGGTGGGGTTTTGTGTAGATGGTTGG         >  SRR19973067.1887076/1‑150 (MQ=60)
                                                                                                                                       TGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGC         >  SRR19973067.3333677/1‑150 (MQ=60)
                                                                                                                                       TGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGC         >  SRR19973067.556777/1‑150 (MQ=60)
                                                                                                                                       TGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGC         >  SRR19973067.3088003/1‑150 (MQ=60)
                                                                                                                                       TGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGC         >  SRR19973067.1885331/1‑150 (MQ=60)
                                                                                                                                       TGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGC         >  SRR19973067.3333703/1‑150 (MQ=60)
                                                                                                                                        GGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCA        >  SRR19973067.2323134/1‑150 (MQ=60)
                                                                                                                                             GATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCAGTACC   >  SRR19973067.1619991/1‑150 (MQ=60)
                                                                                                                                              ATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCAGTACCG  <  SRR19973067.500848/150‑1 (MQ=60)
                                                                                                                                              ATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCAGTACCG  <  SRR19973067.500787/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                
CATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCAGTACCG  >  CP009273/836985‑837276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑