Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 876,395 G→T 93.7% M71I (ATG→ATT)  dacC → D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273876,3950GT93.7% 521.1 / 24.6 184M71I (ATG→ATT) dacCD‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)
Reads supporting (aligned to +/- strand):  ref base G (6/6);  new base T (68/104);  total (74/110)
Fisher's exact test for biased strand distribution p-value = 5.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

AACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTT  >  CP009273/876251‑876539
                                                                                                                                                |                                                                                                                                                
aaCGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTAcc                                                                                                                                               <  1:835576/148‑1 (MQ=255)
 aCGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCa                                                                                                                                              >  1:3207054/1‑148 (MQ=255)
  cGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAg                                                                                                                                             >  2:349655/1‑148 (MQ=255)
    gCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCGGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCt                                                                                                                                           <  1:1841034/148‑1 (MQ=255)
    gCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCt                                                                                                                                           <  1:2027872/148‑1 (MQ=255)
     cATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTa                                                                                                                                          <  1:1970666/148‑1 (MQ=255)
      aTTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTAt                                                                                                                                         <  2:3443120/148‑1 (MQ=255)
      aTTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTAt                                                                                                                                         >  1:1593611/1‑148 (MQ=255)
      aTTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTAt                                                                                                                                         <  2:3021356/148‑1 (MQ=255)
        tCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTAtgt                                                                                                                                       >  1:1754031/1‑148 (MQ=255)
          gcggcgGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTgg                                                                                                                                     <  2:3464051/148‑1 (MQ=255)
           cggcggAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGt                                                                                                                                    <  2:2905111/148‑1 (MQ=255)
           cggcggAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGt                                                                                                                                    <  2:1612701/148‑1 (MQ=255)
               ggAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTggg                                                                                                                                <  1:711532/148‑1 (MQ=255)
                  aCAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAg                                                                                                                             <  1:344188/148‑1 (MQ=255)
                    aaaCCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGgc                                                                                                                           >  1:514529/1‑148 (MQ=255)
                            gAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAgg                                                                                                                   <  1:3112291/148‑1 (MQ=255)
                             aaGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGc                                                                                                                  >  1:3462072/1‑148 (MQ=255)
                                cgcCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGa                                                                                                               <  2:2434637/148‑1 (MQ=255)
                                cgcCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGa                                                                                                               >  1:3209919/1‑148 (MQ=255)
                                cgcCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGa                                                                                                               <  1:2791254/148‑1 (MQ=255)
                                  ccGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATa                                                                                                             <  1:3303127/148‑1 (MQ=255)
                                     aGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGa                                                                                                          <  1:2101437/148‑1 (MQ=255)
                                           gttGCGCGTTCTTGGATTTTAATGGATTACGCCAGGTGTAAAGTGGTGGAAGAAGGAATTGCGGATGAGAAACTGGATCCCGCGAGCCTGAATAAAATCTTTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGTTTAAAc                                                                                                    <  1:2846275/146‑1 (MQ=255)
                                           gATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAAc                                                                                                    <  1:3416411/148‑1 (MQ=255)
                                           gATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAAc                                                                                                    >  1:309472/1‑148 (MQ=255)
                                           gATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAAc                                                                                                    >  1:1133254/1‑148 (MQ=255)
                                            aTGCGCGTTCATGTATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACt                                                                                                   <  1:3136588/148‑1 (MQ=255)
                                             tGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTc                                                                                                  <  1:3533814/148‑1 (MQ=255)
                                              gcgcGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCa                                                                                                 <  2:1688859/148‑1 (MQ=255)
                                                 cgTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCAcca                                                                                              >  1:1315684/1‑147 (MQ=255)
                                                  gTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCAccaa                                                                                             <  2:2897739/148‑3 (MQ=255)
                                                   tGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAat                                                                                            <  1:1425360/148‑1 (MQ=255)
                                                    gCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAata                                                                                           <  1:2916235/148‑1 (MQ=255)
                                                    gCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAata                                                                                           <  2:3052679/148‑1 (MQ=255)
                                                      aTGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATg                                                                                         <  2:1864583/148‑1 (MQ=255)
                                                         gATTTAAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGCCTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGAGAATTTTAAACTCACCAATATGGTg                                                                                      >  2:948941/1‑148 (MQ=255)
                                                          aTTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGa                                                                                     >  2:809870/1‑148 (MQ=255)
                                                            tttAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcg                                                                                   <  1:2243712/148‑1 (MQ=255)
                                                            tttAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcg                                                                                   >  2:1516059/1‑148 (MQ=255)
                                                            tttAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcg                                                                                   <  1:601620/148‑1 (MQ=255)
                                                                 tGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAcggtcgg                                                                              <  2:663541/148‑1 (MQ=255)
                                                                   gATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTa                                                                            >  1:2767023/1‑148 (MQ=255)
                                                                     ttACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTaaa                                                                          >  2:609241/1‑148 (MQ=255)
                                                                     ttACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTaaa                                                                          >  2:1370312/1‑148 (MQ=255)
                                                                     ttACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTaaa                                                                          <  1:841773/148‑1 (MQ=255)
                                                                      tACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAg                                                                         <  2:1295918/148‑1 (MQ=255)
                                                                        cGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGAt                                                                       >  2:1683109/1‑148 (MQ=255)
                                                                            aGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCt                                                                   <  1:3491009/148‑1 (MQ=255)
                                                                            aGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCt                                                                   <  1:710809/148‑1 (MQ=255)
                                                                             gCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTg                                                                  <  2:1808886/148‑1 (MQ=255)
                                                                             gCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTg                                                                  <  2:2815493/148‑1 (MQ=255)
                                                                              cGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTgg                                                                 <  2:3310815/148‑1 (MQ=255)
                                                                              cGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTgg                                                                 <  2:3311368/148‑1 (MQ=255)
                                                                                gTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGc                                                               >  2:1902067/1‑148 (MQ=255)
                                                                                 tAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCg                                                              >  2:825326/1‑148 (MQ=255)
                                                                                 tAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCg                                                              >  1:2373019/1‑148 (MQ=255)
                                                                                  aaaGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGa                                                             >  2:2050311/1‑148 (MQ=255)
                                                                                    aGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACg                                                           >  2:2859321/1‑148 (MQ=255)
                                                                                    aGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACg                                                           <  1:209756/148‑1 (MQ=255)
                                                                                     gTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACgg                                                          >  1:3499304/1‑148 (MQ=255)
                                                                                      tGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACggg                                                         >  2:948087/1‑148 (MQ=255)
                                                                                       gCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGa                                                        >  2:871873/1‑148 (MQ=255)
                                                                                        cTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGaa                                                       <  2:1449357/148‑1 (MQ=255)
                                                                                          ggCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAAt                                                     <  2:2469819/148‑1 (MQ=255)
                                                                                           gCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATc                                                    <  2:2034206/148‑1 (MQ=255)
                                                                                            cAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATcc                                                   <  1:2258689/148‑1 (MQ=255)
                                                                                                aGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCa                                               <  2:1590740/148‑1 (MQ=255)
                                                                                                aGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCa                                               <  2:1671644/148‑1 (MQ=255)
                                                                                                 ggCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCAc                                              <  1:3354339/148‑1 (MQ=255)
                                                                                                   cAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTg                                            >  1:1062612/1‑148 (MQ=255)
                                                                                                   cAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTg                                            <  1:2596144/148‑1 (MQ=255)
                                                                                                     aTGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCg                                          <  2:19976/148‑1 (MQ=255)
                                                                                                            tGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCa                                   <  1:1335097/148‑1 (MQ=255)
                                                                                                            tGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCa                                   <  1:954676/148‑1 (MQ=255)
                                                                                                             gagaAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCTATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCAt                                  <  2:232374/148‑1 (MQ=255)
                                                                                                             gagaAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCAt                                  <  1:2125151/148‑1 (MQ=255)
                                                                                                              agaAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATc                                 >  1:360858/1‑148 (MQ=255)
                                                                                                               gaAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCg                                <  1:1078985/148‑1 (MQ=255)
                                                                                                               gaAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCg                                <  1:995583/148‑1 (MQ=255)
                                                                                                                   cTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTaa                            >  2:1304237/1‑148 (MQ=255)
                                                                                                                   cTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTaa                            >  1:38886/1‑148 (MQ=255)
                                                                                                                    tGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAAt                           >  1:1481388/1‑148 (MQ=255)
                                                                                                                    tGGATCCCGCGAGCCTGACTAAAATCATTACCAGCGATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAACTCCGGCACTGCGTGGTTCATCGGTAAt                           >  1:3248133/1‑148 (MQ=255)
                                                                                                                     ggATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATg                          <  2:1866697/148‑1 (MQ=255)
                                                                                                                        tCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTc                       <  1:2512399/148‑1 (MQ=255)
                                                                                                                        tCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTc                       <  1:2512525/148‑1 (MQ=255)
                                                                                                                        tCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTc                       >  1:585567/1‑148 (MQ=255)
                                                                                                                           cgcgAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTc                    <  2:3511415/148‑1 (MQ=255)
                                                                                                                           cgcgAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTc                    <  2:1754031/148‑1 (MQ=255)
                                                                                                                             cgAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCaa                  >  2:3194017/1‑148 (MQ=255)
                                                                                                                               aGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAAc                <  2:722383/148‑1 (MQ=255)
                                                                                                                                  cTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCgg             <  1:794166/148‑1 (MQ=255)
                                                                                                                                      cTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGa         <  1:1630689/148‑1 (MQ=255)
                                                                                                                                       tAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGAt        >  1:2382588/1‑148 (MQ=255)
                                                                                                                                        aaaaTCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATc       <  1:2359246/148‑1 (MQ=255)
                                                                                                                                        aaaaTCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATc       <  2:1079949/148‑1 (MQ=255)
                                                                                                                                           aTCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAgg    <  2:2170098/148‑1 (MQ=255)
                                                                                                                                            tCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGt   >  2:3428196/1‑148 (MQ=255)
                                                                                                                                             cATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGtt  >  2:3319001/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTT  >  CP009273/876251‑876539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCCCAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCG  >  CP009273/876246‑876542
                                                                                                                                                     |                                                                                                                                                   
GCCCCAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATT                                                                                                                                                     >  SRR19973067.2833626/1‑150 (MQ=60)
GCCCCAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATT                                                                                                                                                     >  SRR19973067.2833723/1‑150 (MQ=60)
GCCCCAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATT                                                                                                                                                     >  SRR19973067.1821110/1‑150 (MQ=60)
    CAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCA                                                                                                                                                 >  SRR19973067.3227267/1‑150 (MQ=60)
     AACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAG                                                                                                                                                <  SRR19973067.841257/150‑1 (MQ=60)
         GCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCGGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTAT                                                                                                                                            <  SRR19973067.1853593/150‑1 (MQ=60)
         GCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTAT                                                                                                                                            <  SRR19973067.2041472/150‑1 (MQ=60)
         GCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTAT                                                                                                                                            <  SRR19973067.2041476/150‑1 (MQ=60)
         GCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTAT                                                                                                                                            >  SRR19973067.1604535/1‑150 (MQ=60)
          CATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATG                                                                                                                                           <  SRR19973067.827753/150‑1 (MQ=60)
          CATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATG                                                                                                                                           <  SRR19973067.1984029/150‑1 (MQ=60)
           ATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGT                                                                                                                                          >  SRR19973067.2396064/1‑150 (MQ=60)
           ATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGT                                                                                                                                          >  SRR19973067.3533654/1‑150 (MQ=60)
           ATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGT                                                                                                                                          >  SRR19973067.1765981/1‑150 (MQ=60)
                    GGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCA                                                                                                                                 <  SRR19973067.716460/150‑1 (MQ=60)
                       ACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGC                                                                                                                              >  SRR19973067.518023/1‑150 (MQ=60)
                       ACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGC                                                                                                                              <  SRR19973067.346564/150‑1 (MQ=60)
                          AACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCT                                                                                                                           >  SRR19973067.1087343/1‑150 (MQ=60)
                          AACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCT                                                                                                                           >  SRR19973067.608807/1‑150 (MQ=60)
                            CCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTA                                                                                                                         <  SRR19973067.374213/150‑1 (MQ=60)
                               TTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGG                                                                                                                      <  SRR19973067.144597/150‑1 (MQ=60)
                                TGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGC                                                                                                                     >  SRR19973067.3484080/1‑150 (MQ=60)
                                TGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGATGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGC                                                                                                                     <  SRR19973067.324623/150‑1 (MQ=60)
                                 GAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCC                                                                                                                    <  SRR19973067.3132079/150‑1 (MQ=60)
                                   AGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGA                                                                                                                  >  SRR19973067.3230145/1‑150 (MQ=60)
                                     CGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATA                                                                                                                <  SRR19973067.2809269/150‑1 (MQ=60)
                                     CGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATA                                                                                                                <  SRR19973067.2136228/150‑1 (MQ=60)
                                     CGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATA                                                                                                                <  SRR19973067.2260092/150‑1 (MQ=60)
                                       CCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAG                                                                                                              <  SRR19973067.3323933/150‑1 (MQ=60)
                                       CCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAG                                                                                                              <  SRR19973067.3323951/150‑1 (MQ=60)
                                        CGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGA                                                                                                             >  SRR19973067.3460259/1‑150 (MQ=60)
                                          AGCGTGGATTCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATT                                                                                                           <  SRR19973067.2115375/150‑1 (MQ=60)
                                             GTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAA                                                                                                        >  SRR19973067.3186021/1‑150 (MQ=60)
                                              TGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAAC                                                                                                       >  SRR19973067.1140891/1‑150 (MQ=60)
                                              TGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAAC                                                                                                       >  SRR19973067.311491/1‑150 (MQ=60)
                                                GTTGCGCGTTCTTGGATTTTAATGGATTACGCCAGGTGTAAAGTGGTGGAAGAAGGAATTGCGGATGAGAAACTGGATCCCGCGAGCCTGAATAAAATCTTTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGTTTAAACTC                                                                                                     <  SRR19973067.2864543/150‑1 (MQ=60)
                                                GATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTC                                                                                                     <  SRR19973067.3438156/150‑1 (MQ=60)
                                                 ATGCGCGTTCATGTATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCA                                                                                                    <  SRR19973067.3156507/150‑1 (MQ=60)
                                                  TGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCAC                                                                                                   <  SRR19973067.3556152/150‑1 (MQ=60)
                                                  TGCGAGTGGATTGATTTTAATGGATTACGCCAGCGGTATAGTGCTGTCAGTAGGCAATGCGTATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGAGCTTAAGGCCGATAAGATTAAACTCAC                                                                                                   <  SRR19973067.74375/150‑1 (MQ=60)
                                                    CGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCA                                                                                                 >  SRR19973067.1326399/1‑150 (MQ=60)
                                                    CGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCA                                                                                                 >  SRR19973067.1324668/1‑150 (MQ=60)
                                                    CGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCA                                                                                                 >  SRR19973067.3407817/1‑150 (MQ=60)
                                                     GCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAA                                                                                                >  SRR19973067.3118337/1‑150 (MQ=60)
                                                        TGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATAT                                                                                             <  SRR19973067.1434983/150‑1 (MQ=60)
                                                         GCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATG                                                                                            <  SRR19973067.2934795/150‑1 (MQ=60)
                                                         GCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATG                                                                                            <  SRR19973067.2933398/150‑1 (MQ=60)
                                                          CATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGG                                                                                           >  SRR19973067.2202310/1‑150 (MQ=60)
                                                                 TTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGT                                                                                    <  SRR19973067.2258622/150‑1 (MQ=60)
                                                                 TTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGT                                                                                    <  SRR19973067.605421/150‑1 (MQ=60)
                                                                   TAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCG                                                                                  <  SRR19973067.1269533/150‑1 (MQ=60)
                                                                    AATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGTTGACGGTCGG                                                                                 >  SRR19973067.3144799/1‑150 (MQ=60)
                                                                      TGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTA                                                                               >  SRR19973067.2784935/1‑150 (MQ=60)
                                                                      TGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTA                                                                               >  SRR19973067.2784902/1‑150 (MQ=60)
                                                                       GGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAA                                                                              >  SRR19973067.2581311/1‑150 (MQ=60)
                                                                          TTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGA                                                                           <  SRR19973067.1690614/150‑1 (MQ=60)
                                                                          TTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGA                                                                           <  SRR19973067.847485/150‑1 (MQ=60)
                                                                                 AGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGG                                                                    <  SRR19973067.3513167/150‑1 (MQ=60)
                                                                                 AGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGG                                                                    <  SRR19973067.715735/150‑1 (MQ=60)
                                                                                 AGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGG                                                                    <  SRR19973067.715753/150‑1 (MQ=60)
                                                                                    GGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCG                                                                 >  SRR19973067.2279451/1‑150 (MQ=60)
                                                                                    GGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCG                                                                 >  SRR19973067.2388454/1‑150 (MQ=60)
                                                                                        AAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGG                                                             >  SRR19973067.3521476/1‑150 (MQ=60)
                                                                                        AAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGG                                                             >  SRR19973067.3521495/1‑150 (MQ=60)
                                                                                         AGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGG                                                            <  SRR19973067.211392/150‑1 (MQ=60)
                                                                                         AGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGG                                                            <  SRR19973067.211325/150‑1 (MQ=60)
                                                                                                 CAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGG                                                    <  SRR19973067.2273676/150‑1 (MQ=60)
                                                                                                 CAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGG                                                    <  SRR19973067.1627842/150‑1 (MQ=60)
                                                                                                 CAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGG                                                    <  SRR19973067.3327201/150‑1 (MQ=60)
                                                                                                      GGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTG                                               >  SRR19973067.1069904/1‑150 (MQ=60)
                                                                                                      GGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTG                                               <  SRR19973067.3375626/150‑1 (MQ=60)
                                                                                                        CAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCG                                             <  SRR19973067.2613033/150‑1 (MQ=60)
                                                                                                          ATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTG                                           <  SRR19973067.188151/150‑1 (MQ=60)
                                                                                                                 TGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATC                                    >  SRR19973067.363360/1‑150 (MQ=60)
                                                                                                                 TGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATC                                    <  SRR19973067.961213/150‑1 (MQ=60)
                                                                                                                 TGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATC                                    >  SRR19973067.363311/1‑150 (MQ=60)
                                                                                                                 TGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATC                                    <  SRR19973067.1344232/150‑1 (MQ=60)
                                                                                                                 TGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATC                                    <  SRR19973067.1344212/150‑1 (MQ=60)
                                                                                                                  GAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCG                                   <  SRR19973067.2139333/150‑1 (MQ=60)
                                                                                                                  GAGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCG                                   <  SRR19973067.984950/150‑1 (MQ=60)
                                                                                                                   AGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGG                                  >  SRR19973067.905901/1‑150 (MQ=60)
                                                                                                                   AGAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGG                                  >  SRR19973067.2629866/1‑150 (MQ=60)
                                                                                                                    GAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGT                                 <  SRR19973067.1086377/150‑1 (MQ=60)
                                                                                                                    GAAACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGT                                 <  SRR19973067.1002436/150‑1 (MQ=60)
                                                                                                                      AACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAA                               >  SRR19973067.3494097/1‑150 (MQ=60)
                                                                                                                      AACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAA                               >  SRR19973067.39442/1‑150 (MQ=60)
                                                                                                                      AACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAA                               >  SRR19973067.1852534/1‑150 (MQ=60)
                                                                                                                       ACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAAT                              >  SRR19973067.1491220/1‑150 (MQ=60)
                                                                                                                       ACTGGATCCCGCGAGCCTGACTAAAATCATTACCAGCGATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAACTCCGGCACTGCGTGGTTCATCGGTAAT                              >  SRR19973067.3268468/1‑150 (MQ=60)
                                                                                                                           GATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTC                          >  SRR19973067.589309/1‑150 (MQ=60)
                                                                                                                           GATCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTC                          >  SRR19973067.589407/1‑150 (MQ=60)
                                                                                                                             TCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCT                        <  SRR19973067.2528882/150‑1 (MQ=60)
                                                                                                                             TCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCT                        <  SRR19973067.2528883/150‑1 (MQ=60)
                                                                                                                             TCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCT                        <  SRR19973067.2528971/150‑1 (MQ=60)
                                                                                                                             TCCCGCGAGCCTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCT                        <  SRR19973067.2529009/150‑1 (MQ=60)
                                                                                                                                       CTGACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGC              <  SRR19973067.799646/150‑1 (MQ=60)
                                                                                                                                          ACTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGAT           >  SRR19973067.2398057/1‑150 (MQ=60)
                                                                                                                                           CTAAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATC          <  SRR19973067.1641911/150‑1 (MQ=60)
                                                                                                                                             AAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAG        <  SRR19973067.2374627/150‑1 (MQ=60)
                                                                                                                                             AAAATCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAG        >  SRR19973067.3086565/1‑150 (MQ=60)
                                                                                                                                                 TCATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTT    <  SRR19973067.535748/150‑1 (MQ=60)
                                                                                                                                                  CATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTC   >  SRR19973067.526726/1‑150 (MQ=60)
                                                                                                                                                  CATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTC   >  SRR19973067.2593574/1‑150 (MQ=60)
                                                                                                                                                   ATTACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCG  >  SRR19973067.2272517/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GCCCCAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCG  >  CP009273/876246‑876542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑