Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,082,056 G→T 43.5% S416* (TCA→TAA)  pgaC ← biofilm PGA synthase PgaCD, catalytic subunit; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase; c‑di‑GMP‑stimulated activity and dimerization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,082,0560GT43.5% 167.0 / 238.6 205S416* (TCA→TAA) pgaCbiofilm PGA synthase PgaCD, catalytic subunit; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase; c‑di‑GMP‑stimulated activity and dimerization
Reads supporting (aligned to +/- strand):  ref base G (49/67);  new base T (44/45);  total (93/112)
Fisher's exact test for biased strand distribution p-value = 3.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01

Only 100 of 209 total aligned reads displayed.

TTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTAAACATAACGTACACAATAATA  >  CP009273/1081909‑1082203
                                                                                                                                                   |                                                                                                                                                   
ttGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATg                                                                                                                                                     <  1:667223/148‑1 (MQ=255)
ttGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATg                                                                                                                                                     <  2:1562258/148‑1 (MQ=255)
            aaTCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCg                                                                                                                                         >  2:1805806/1‑148 (MQ=255)
            aaTCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCg                                                                                                                                         <  1:1560433/148‑1 (MQ=255)
            aaTCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCg                                                                                                                                         >  1:1022774/1‑148 (MQ=255)
              tCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGtt                                                                                                                                       <  1:2771511/148‑1 (MQ=255)
               cAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTg                                                                                                                                      >  2:1034771/1‑148 (MQ=255)
               cAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTg                                                                                                                                      <  2:2789565/148‑1 (MQ=255)
                 aCCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGcc                                                                                                                                    >  2:2047981/1‑148 (MQ=255)
                    aGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAgg                                                                                                                                 <  1:955657/148‑1 (MQ=255)
                       aaaCGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACATACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTc                                                                                                                              >  1:3282090/1‑148 (MQ=255)
                        aaCGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCa                                                                                                                             >  2:522291/1‑148 (MQ=255)
                         aCGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAg                                                                                                                            >  2:2668083/1‑148 (MQ=255)
                          cGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGc                                                                                                                           <  2:1775383/148‑1 (MQ=255)
                          cGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGc                                                                                                                           >  2:2996488/1‑148 (MQ=255)
                            tACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCACTGTCGTTGCCAGGCTCAGCAt                                                                                                                         >  2:3473248/1‑148 (MQ=255)
                            tACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCAt                                                                                                                         <  2:3124509/148‑1 (MQ=255)
                            tACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCAt                                                                                                                         <  2:2582723/148‑1 (MQ=255)
                             aCTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATc                                                                                                                        <  1:3196748/148‑1 (MQ=255)
                               tGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTATTCATCAACATGACTAGTGTAAATGATACCAAAGTCGTTGCCAGGCTCAGCATCCa                                                                                                                      >  1:534312/1‑148 (MQ=255)
                                  tGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGaa                                                                                                                   >  2:231759/1‑148 (MQ=255)
                                    aTTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGaaaa                                                                                                                 >  2:1698970/1‑148 (MQ=255)
                                     ttGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAAt                                                                                                                >  2:1545849/1‑148 (MQ=255)
                                     ttGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAAt                                                                                                                >  2:1356641/1‑148 (MQ=255)
                                      tGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATa                                                                                                               >  1:2644337/1‑148 (MQ=255)
                                           ggTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCgg                                                                                                          >  1:1082542/1‑148 (MQ=255)
                                           ggTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCgg                                                                                                          >  1:2294768/1‑148 (MQ=255)
                                             tCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGa                                                                                                        >  2:1386159/1‑148 (MQ=255)
                                                taataaTTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAAcc                                                                                                     <  1:2062944/148‑1 (MQ=255)
                                                taataaTTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAAcc                                                                                                     >  2:2430188/1‑148 (MQ=255)
                                                 aataatTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCa                                                                                                    >  2:1335189/1‑148 (MQ=255)
                                                  ataatTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCaa                                                                                                   >  2:179076/1‑148 (MQ=255)
                                                   taatTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCaaa                                                                                                  >  2:731791/1‑148 (MQ=255)
                                                   taatTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCaaa                                                                                                  >  2:3282714/1‑148 (MQ=255)
                                                   taatTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCaaa                                                                                                  <  1:2438078/148‑1 (MQ=255)
                                                    aatTAAATTGTTCACATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAAAGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAaat                                                                                                 >  1:126612/1‑148 (MQ=255)
                                                       tAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAaataat                                                                                              >  1:1135107/1‑148 (MQ=255)
                                                         aaTTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATcc                                                                                            <  1:2303872/148‑1 (MQ=255)
                                                            tGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGa                                                                                         >  1:3143343/1‑148 (MQ=255)
                                                                  tatTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCg                                                                                   <  2:118316/148‑1 (MQ=255)
                                                                   atTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGa                                                                                  <  1:2357156/148‑1 (MQ=255)
                                                                    ttAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGAt                                                                                 >  1:2372775/1‑148 (MQ=255)
                                                                     tAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATg                                                                                >  2:938210/1‑148 (MQ=255)
                                                                     tAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATg                                                                                >  1:168112/1‑148 (MQ=255)
                                                                     tAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATg                                                                                >  2:2706537/1‑148 (MQ=255)
                                                                      aaCCTCTCAGAATCCCGCTATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGa                                                                               <  2:628053/148‑1 (MQ=255)
                                                                        ccTCTCAGAATCCCGCGATCGGGACTTACCCTACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAg                                                                             <  2:1864413/148‑1 (MQ=255)
                                                                        ccTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGAGTTGCTTTTTAGGCATCAACATGTCTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAg                                                                             <  1:1157677/148‑1 (MQ=255)
                                                                         ctctCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGt                                                                            <  2:2912783/148‑1 (MQ=255)
                                                                          tctcAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTc                                                                           <  1:2230748/148‑1 (MQ=255)
                                                                               gAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGAtt                                                                      <  2:1484515/148‑1 (MQ=255)
                                                                                aaTCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTa                                                                     <  1:2133612/148‑1 (MQ=255)
                                                                                  tCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGACGTCAGATTATg                                                                   >  2:2879343/1‑148 (MQ=255)
                                                                                  tCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATg                                                                   >  2:3438792/1‑148 (MQ=255)
                                                                                    ccGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCt                                                                 <  1:1650336/148‑1 (MQ=255)
                                                                                     cgcgATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTc                                                                <  1:132791/148‑1 (MQ=255)
                                                                                       cgATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCAt                                                              <  2:2265645/148‑1 (MQ=255)
                                                                                         aTCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAg                                                            <  1:100341/148‑1 (MQ=255)
                                                                                          tCGGGACTTACCCACCGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGc                                                           <  1:148911/148‑1 (MQ=255)
                                                                                          tCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCACTGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGc                                                           <  1:148304/148‑1 (MQ=255)
                                                                                           cGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCg                                                          <  2:409240/148‑1 (MQ=255)
                                                                                           cGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCg                                                          <  2:412499/148‑1 (MQ=255)
                                                                                            gggACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGa                                                         <  1:2996488/148‑1 (MQ=255)
                                                                                            gggACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGa                                                         <  2:2008193/148‑1 (MQ=255)
                                                                                              gACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGAtt                                                       >  1:2201788/1‑148 (MQ=255)
                                                                                               aCTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATtc                                                      >  1:867980/1‑148 (MQ=255)
                                                                                               aCTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATtc                                                      <  1:547339/148‑1 (MQ=255)
                                                                                                cTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATtct                                                     >  1:2403623/1‑148 (MQ=255)
                                                                                                   aCCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGa                                                  <  1:2611234/148‑1 (MQ=255)
                                                                                                      cAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGatca                                               >  1:850400/1‑148 (MQ=255)
                                                                                                       aaCGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGatcat                                              >  1:846422/1‑148 (MQ=255)
                                                                                                        aCGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGatcatc                                             <  2:3282090/148‑1 (MQ=255)
                                                                                                        aCGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGatcatc                                             <  2:2376669/148‑1 (MQ=255)
                                                                                                          gggCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAg                                           <  1:1711946/148‑1 (MQ=255)
                                                                                                          gggCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAg                                           <  1:3355686/148‑1 (MQ=255)
                                                                                                          gggCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAg                                           <  1:2692358/148‑1 (MQ=255)
                                                                                                               cgTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGa                                      <  2:791495/148‑1 (MQ=255)
                                                                                                               cgTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGa                                      >  2:2744567/1‑148 (MQ=255)
                                                                                                                      ttttAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATaaa                               >  2:393226/1‑148 (MQ=255)
                                                                                                                      ttttAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATaaa                               <  1:2871847/148‑1 (MQ=255)
                                                                                                                       tttAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAAt                              <  2:2237925/148‑1 (MQ=255)
                                                                                                                        ttAGGCATCAACATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAAtt                             <  2:123510/148‑1 (MQ=255)
                                                                                                                          aGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGc                           <  1:496490/148‑1 (MQ=255)
                                                                                                                           ggCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCa                          <  2:3297143/148‑1 (MQ=255)
                                                                                                                            gCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAg                         >  1:352737/1‑148 (MQ=255)
                                                                                                                                cAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTaaa                     <  1:2810898/148‑1 (MQ=255)
                                                                                                                                 aaCATGACTCGTGTAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTAAAc                    <  1:2244237/148‑1 (MQ=255)
                                                                                                                                     tGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTaa                      >  2:3384634/1‑142 (MQ=255)
                                                                                                                                     tGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTaa                      <  1:3384634/142‑1 (MQ=255)
                                                                                                                                     tGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTAAACATaa                <  1:1162025/148‑1 (MQ=255)
                                                                                                                                     tGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTAAACATaa                <  1:304734/148‑1 (MQ=255)
                                                                                                                                         tCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTaaa                     <  1:1042457/139‑1 (MQ=255)
                                                                                                                                         tCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTAAACATAACGTa            <  1:829570/148‑1 (MQ=255)
                                                                                                                                          cGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTAAACATAACGTac           <  2:1135107/148‑1 (MQ=255)
                                                                                                                                             gtAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTAAACATAACGTAcaca        <  1:1545849/148‑1 (MQ=255)
                                                                                                                                             gtAAATTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTAAACATAACGTAcaca        <  1:1356641/148‑1 (MQ=255)
                                                                                                                                               aaaTTATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTAAACATAACGTACACaat      >  1:527835/1‑148 (MQ=255)
                                                                                                                                               aaaTGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTAAACATAACGTACACaat      >  1:2845811/1‑148 (MQ=255)
                                                                                                                                               aaaTGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTAAACATAACGTACACaat      >  1:226518/1‑148 (MQ=255)
                                                                                                                                                   gATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTAAACATAACGTACACAataata  <  1:1703673/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAGCATCCAGAAAATAACCGGGAACCAAATAATCCAGAAAAGCGATGAAGTCAGATTATGCTCATAGCGATTCTCGATCATCAGGCTGACAATAAATTGCAGTAAACATAACGTACACAATAATA  >  CP009273/1081909‑1082203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A