Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F368 I0 R1	1472	214.4	7116750	95.9%	6824963	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,139,516	T→A	45.0%	Q103L (CAG→CTG)	rne ←	fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,139,516	0	T	A	45.0%	62.5 / 261.1	178	Q103L (CAG→CTG)	rne	fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein
Reads supporting (aligned to +/- strand): ref base T (52/46); new base A (35/45); total (87/91)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 183 total aligned reads displayed.

TTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAG  >  CP009273/1139369‑1139660

tttAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCt                                                                                                                                                  >  2:3064448/1‑148 (MQ=255)

tttAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCCGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGGGGTTAATGCCGCGCCTTTGTTGCCGCGCACTTCTTTATCGATCt                                                                                                                                                  >  1:1226480/1‑148 (MQ=255)

 ttAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTg                                                                                                                                                 <  2:2803683/148‑1 (MQ=255)

  tAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGa                                                                                                                                                <  1:2264098/148‑1 (MQ=255)

  tAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGa                                                                                                                                                <  1:1744491/148‑1 (MQ=255)

  tAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGa                                                                                                                                                <  2:3108969/148‑1 (MQ=255)

   aaTTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGaa                                                                                                                                               <  1:2294636/148‑1 (MQ=255)

    aTTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAAc                                                                                                                                              >  2:3249931/1‑148 (MQ=255)

    aTTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAAc                                                                                                                                              >  2:2229832/1‑148 (MQ=255)

      tCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTCCCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACaa                                                                                                                                            >  2:3048660/1‑148 (MQ=255)

      tCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACaa                                                                                                                                            >  2:3319740/1‑148 (MQ=255)

      tCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACaa                                                                                                                                            >  2:3181963/1‑148 (MQ=255)

          tACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGAc                                                                                                                                        <  1:783413/148‑1 (MQ=255)

               tcgtcgCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGAGAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCt                                                                                                                                   <  2:935015/148‑1 (MQ=255)

                cgtcgCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTg                                                                                                                                  <  2:3117431/148‑1 (MQ=255)

                 gtcgCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTAACCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGa                                                                                                                                 >  1:155256/1‑148 (MQ=255)

                  tcgCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGAc                                                                                                                                <  2:2255879/148‑1 (MQ=255)

                  tcgCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGAc                                                                                                                                >  1:3002550/1‑148 (MQ=255)

                  tcgCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGAc                                                                                                                                >  2:2636309/1‑148 (MQ=255)

                    gCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCt                                                                                                                              >  1:2210845/1‑148 (MQ=255)

                      cTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTc                                                                                                                            >  1:2381364/1‑148 (MQ=255)

                           aTACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCa                                                                                                                       >  2:566845/1‑148 (MQ=255)

                                 agagaAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACAt                                                                                                                 >  1:1010645/2‑148 (MQ=255)

                                  gagaAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATc                                                                                                                <  2:2125324/148‑1 (MQ=255)

                                   agaAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCt                                                                                                               <  1:3044786/148‑1 (MQ=255)

                                     aaaTGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCttt                                                                                                             <  1:3330451/148‑1 (MQ=255)

                                      aaTGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTa                                                                                                            >  1:540172/1‑148 (MQ=255)

                                      aaTGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTGTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACATTCACGCAACACATCTGTa                                                                                                            >  2:2346816/1‑148 (MQ=255)

                                          ccACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGt                                                                                                        <  2:232380/148‑1 (MQ=255)

                                             cccGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTgg                                                                                                     <  1:1115977/148‑1 (MQ=255)

                                              ccGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTggg                                                                                                    <  2:685748/148‑1 (MQ=255)

                                              ccGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTggg                                                                                                    <  2:1338353/148‑1 (MQ=255)

                                               cgcgcgcgGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGGTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGga                                                                                                   <  2:336704/148‑1 (MQ=255)

                                                   cgcgGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGgacgac                                                                                               <  2:2663206/148‑1 (MQ=255)

                                                       ggTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATg                                                                                           <  1:537128/148‑1 (MQ=255)

                                                        gttgttCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGa                                                                                          <  2:875635/148‑1 (MQ=255)

                                                         ttgttCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAg                                                                                         >  2:3266197/1‑148 (MQ=255)

                                                          tgttCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGc                                                                                        >  1:2796525/1‑148 (MQ=255)

                                                           gttCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGga                                                                                                   >  1:2999976/1‑136 (MQ=255)

                                                           gttCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGga                                                                                                   <  2:159258/136‑1 (MQ=255)

                                                           gttCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCa                                                                                       >  2:2871936/1‑148 (MQ=255)

                                                           gttCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCa                                                                                       >  2:2871897/1‑148 (MQ=255)

                                                              cGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTg                                                                                    <  1:2788516/148‑1 (MQ=255)

                                                              cGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTg                                                                                    <  1:1008887/148‑1 (MQ=255)

                                                                   tCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGtt                                                                               >  2:1450372/1‑148 (MQ=255)

                                                                       aaCCAGATCGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCa                                                                           <  2:1832716/148‑1 (MQ=255)

                                                                       aaCCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCa                                                                           >  1:2940708/1‑148 (MQ=255)

                                                                          cAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAggg                                                                        >  1:162700/1‑148 (MQ=255)

                                                                            gATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGaa                                                                      >  1:489211/1‑148 (MQ=255)

                                                                             aTAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGaaa                                                                     <  1:93836/148‑1 (MQ=255)

                                                                              tAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAat                                                                    <  2:50053/148‑1 (MQ=255)

                                                                                  tACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTc                                                                <  1:643978/148‑1 (MQ=255)

                                                                                   aCCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTcg                                                               >  1:38910/1‑148 (MQ=255)

                                                                                   aCCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTcg                                                               >  2:3428502/1‑148 (MQ=255)

                                                                                        ccAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGc                                                          <  1:3214779/148‑1 (MQ=255)

                                                                                         cAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCa                                                         >  2:115454/1‑148 (MQ=255)

                                                                                           gACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAAt                                                       <  2:2057575/148‑1 (MQ=255)

                                                                                            aCTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAAtt                                                      >  1:1382871/1‑148 (MQ=255)

                                                                                             cTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAAttt                                                     <  2:1783425/148‑1 (MQ=255)

                                                                                              tGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTc                                                    >  1:857617/1‑148 (MQ=255)

                                                                                               gATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCt                                                   <  2:47239/148‑1 (MQ=255)

                                                                                                aTAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCtt                                                  <  2:1722433/148‑1 (MQ=255)

                                                                                                aTAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCtt                                                  <  2:1070940/148‑1 (MQ=255)

                                                                                                aTAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTGAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCtt                                                  <  2:1071065/148‑1 (MQ=255)

                                                                                                aTAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCtt                                                  <  2:2198066/148‑1 (MQ=255)

                                                                                                           tAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGggagg                                       >  2:2270610/1‑148 (MQ=255)

                                                                                                            aaTGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGggagga                                      <  2:2945446/148‑1 (MQ=255)

                                                                                                              tGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGaaa                                    <  1:2820484/148‑1 (MQ=255)

                                                                                                              tGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGaaa                                    >  1:1239043/1‑148 (MQ=255)

                                                                                                                  gcgcCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGt                                <  2:1463185/148‑1 (MQ=255)

                                                                                                                   cgcCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTg                               >  1:2120558/1‑148 (MQ=255)

                                                                                                                   cgcCTTTGTTGCCGCGCTCTTCTGTATCGATCTGAACACTGACTTCCTGACCTTCACGCAACACATCTTTAAGGGTGGGACGACCATGAGCACTGTAGTTAGCGGGGAAATAGTCGCGGGCAATTTCGGTTAGTGGGAGGAAACCGTg                               >  1:327872/1‑148 (MQ=255)

                                                                                                                      cTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACg                            >  1:2647736/1‑148 (MQ=255)

                                                                                                                      cTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACg                            >  1:2835/1‑148 (MQ=255)

                                                                                                                        ttgttgCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGtt                          <  1:802893/148‑1 (MQ=255)

                                                                                                                           ttgCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAg                       <  1:371923/148‑1 (MQ=255)

                                                                                                                            tgCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAgc                      >  1:2573710/1‑148 (MQ=255)

                                                                                                                            tgCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAgc                      >  1:1014757/1‑148 (MQ=255)

                                                                                                                              ccGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAgcgc                    <  1:1040466/148‑1 (MQ=255)

                                                                                                                              ccGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAgcgc                    <  1:180970/148‑1 (MQ=255)

                                                                                                                                  gcTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTa                >  2:379423/1‑148 (MQ=255)

                                                                                                                                  gcTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTACTGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTa                <  1:1951745/148‑1 (MQ=255)

                                                                                                                                   ctctTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTaa               <  1:780843/148‑1 (MQ=255)

                                                                                                                                    tcttctTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAAt              >  2:2436440/1‑148 (MQ=255)

                                                                                                                                    tcttctTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAAt              >  2:2092630/1‑148 (MQ=255)

                                                                                                                                    tcttctTTATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAAt              <  1:2636309/148‑1 (MQ=255)

                                                                                                                                     cttcttTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATc             >  2:1576683/1‑148 (MQ=255)

                                                                                                                                        cttTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATcaac          >  1:3064360/1‑148 (MQ=255)

                                                                                                                                         tttATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATcaaca         <  2:540172/148‑1 (MQ=255)

                                                                                                                                         tttATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATcaaca         >  1:2725057/1‑148 (MQ=255)

                                                                                                                                         tttATCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATcaaca         >  1:3438363/1‑148 (MQ=255)

                                                                                                                                          ttATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATcaacaa        >  2:1211048/1‑148 (MQ=255)

                                                                                                                                            aTCGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACaaaa      >  1:3326894/1‑148 (MQ=255)

                                                                                                                                              cGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAagc    >  1:2754680/1‑148 (MQ=255)

                                                                                                                                              cGATCAGAACAATGACTTCCTGACCTTCACGCAACACCTCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAagc    <  1:566845/148‑1 (MQ=255)

                                                                                                                                              cGATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAagc    <  2:2897901/148‑1 (MQ=255)

                                                                                                                                               gATCAGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAagca   >  2:3003446/1‑148 (MQ=255)

                                                                                                                                                aTCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAagcag  >  2:2897142/1‑148 (MQ=255)

                                                                                                                                                aTCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAagcag  <  1:2659447/148‑1 (MQ=255)

                                                                                                                                                aTCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAagcag  <  1:67649/148‑1 (MQ=255)

TTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAG  >  CP009273/1139369‑1139660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A