Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,364,366 G→A 9.6% intergenic (+106/‑107) pspE → / → ycjM thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,364,3660GA 9.6% 671.5 / 41.9 209intergenic (+106/‑107)pspE/ycjMthiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand):  ref base G (72/115);  new base A (5/15);  total (79/130)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

GTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGG  >  CP009273/1364220‑1364513
                                                                                                                                                  |                                                                                                                                                   
gTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTTTCACACCGTATTTTCATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTgg                                                                                                                                                    >  2:1780125/1‑148 (MQ=255)
gTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTgg                                                                                                                                                    <  1:1466265/148‑1 (MQ=255)
   gCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTATTTCAAACCTTAATTAAAACACCATAGATTATTTTCGTAAGATCACAAAAACGATTTTTAACAGGTaa                                                                                                                                                 >  2:1496005/1‑148 (MQ=255)
    ccTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTGTGTCACACCATATTTTAATACCCCGAGATTATTTGCGTCAGCTCACCAATAAGCTTTTTACCGTGTaaa                                                                                                                                                >  1:2073541/1‑148 (MQ=255)
      tGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaa                                                                                                                                              <  1:737057/148‑1 (MQ=255)
        aaaGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTGTGTGACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCGCCCAAATACGCTTTTTCCCTGGTTAAAAAt                                                                                                                                            >  2:3038178/1‑148 (MQ=255)
          aGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGa                                                                                                                                          <  1:882227/148‑1 (MQ=255)
          aGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGa                                                                                                                                          <  2:808301/148‑1 (MQ=255)
            aCATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGAtt                                                                                                                                        <  1:2653535/148‑1 (MQ=255)
            aCATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGAtt                                                                                                                                        <  1:3157246/148‑1 (MQ=255)
             cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGAttt                                                                                                                                       <  2:2813792/148‑1 (MQ=255)
               tCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTAACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTcc                                                                                                                                     <  2:3097361/148‑1 (MQ=255)
                    aTGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTGTGTGACCCCTTATTTACATCCCCATAGCTTATTTGCGTCAGCTCACAAATTCGCTTTTTGCCTGGTGAAAAATTTTTTccggggt                                                                                                                                >  2:2164785/1‑143 (MQ=255)
                    aTGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTGACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCGCACCAATACGCATTTTGCCTGGTAAAAAATTATTTACTGCGt                                                                                                                                >  2:681971/1‑148 (MQ=255)
                      gCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGa                                                                                                                              <  2:1210203/148‑1 (MQ=255)
                      gCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGa                                                                                                                              <  1:112358/148‑1 (MQ=255)
                           aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTaaa                                                                                                                         <  1:1575446/148‑1 (MQ=255)
                           aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTAGTAAAAAATGATTTCCTGCGTGACTaaa                                                                                                                         <  2:392925/148‑1 (MQ=255)
                                 aaGGTTAAAAGAATTCACCATGAGCGGCGCGTATGCGCCGTTTTTTTTTATTTGACACCTTATTTACATACCCCGAGATTATTTTCGTCAGCTCACAAAAAAGCATTTTTCCTGGTAAAAAATTTTTTCCTGCGTGTCTaaaaaacgt                                                                                                                   >  1:1479831/1‑143 (MQ=255)
                                  aGGTTAAAAGAATTCACCATGAGCGGCGATAATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtg                                                                                                                  <  2:2931132/148‑1 (MQ=255)
                                   ggTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgt                                                                                                                 <  1:421661/148‑1 (MQ=255)
                                       aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTGTGTCACACCAGATTTAAAACACCAGAGATTATTTTCGTCAGCTCAACAATAACCATTTTACCTGGGAAAAAATTATTTAATGGGTGAATAAAAACCCTGTGcgc                                                                                                             >  2:1249660/1‑146 (MQ=255)
                                       aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGTGTTTTTTTCTGTCACACCGTATTTAAAACCCCAAAGATTATTTTCGTCAGCTCACCAATAAGCTTTTTCCCGGGTAAAAAATGATTTACTGCGGTACTAAAACCCTTGTGCTc                                                                                                             >  2:3507673/1‑148 (MQ=255)
                                        aaaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCATATTTAAATACCCAAAGATTATTTTCGTCAGCTAACAAAAAAGCATTTTACCGGGTAAAAAAATATTTACTGCGTGGCAAAAACCCATGTTCTCa                                                                                                            >  2:3062619/1‑148 (MQ=255)
                                         aaGAATTCACCCTGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCaa                                                                                                           <  1:201414/148‑1 (MQ=255)
                                         aaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCaa                                                                                                           <  1:806125/148‑1 (MQ=255)
                                         aaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCaa                                                                                                           <  1:3388648/148‑1 (MQ=255)
                                         aaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCaa                                                                                                           <  1:1193912/148‑1 (MQ=255)
                                          aGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAAt                                                                                                          <  1:1614528/148‑1 (MQ=255)
                                          aGAAGTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAAt                                                                                                          <  2:2371275/148‑1 (MQ=255)
                                                 aCCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGt                                                                                                   <  2:3023622/148‑1 (MQ=255)
                                                 aCCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCATATTTAAATACCCAAAGATTATTTGCGTCACCTCACAAATAAGCTTTTTCCCAGGTAAAAAATGATTTCCTGAGTGAATAAAAACCATGTGCTCAAATGACAGt                                                                                                   >  2:2005007/1‑148 (MQ=255)
                                                  ccATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGtt                                                                                                  <  2:260362/148‑1 (MQ=255)
                                                  ccATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGtt                                                                                                  <  2:3352442/148‑1 (MQ=255)
                                                  ccATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGtt                                                                                                  <  2:83363/148‑1 (MQ=255)
                                                  ccATGAGCGGCGCGTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTAGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGtt                                                                                                  <  1:2997919/148‑1 (MQ=255)
                                                   cATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGttt                                                                                                 <  2:1929521/148‑1 (MQ=255)
                                                   cATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACAGCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGttt                                                                                                 <  2:133988/148‑1 (MQ=255)
                                                      gAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtt                                                                                              <  1:1912936/148‑1 (MQ=255)
                                                        gcggcgCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtttt                                                                                            <  1:1337683/148‑1 (MQ=255)
                                                          ggcgCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCt                                                                                          <  1:922713/148‑1 (MQ=255)
                                                                 aTGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCATTGTGCTCAATTGACAGTTTATTTTCTGCGGagt                                                                                   <  1:2032676/148‑1 (MQ=255)
                                                                       cGTTTTTTTTTCTGTCACACCTTATTTACATCCCCCTAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGtctc                                                                             <  1:3260257/148‑1 (MQ=255)
                                                                           tttttttCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGt                                                                         <  2:3145697/148‑1 (MQ=255)
                                                                            ttttttCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGtt                                                                        <  2:2567876/148‑1 (MQ=255)
                                                                               tttCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCa                                                                     >  1:3180695/1‑148 (MQ=255)
                                                                                   tGTCACACCTTATTTACATCCCCATAGATTATTTGAGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATggg                                                                 <  1:2003082/148‑1 (MQ=255)
                                                                                      cacaCCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGAcc                                                              >  2:1753009/1‑148 (MQ=255)
                                                                                           cTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTAc                                                         >  1:3481556/1‑148 (MQ=255)
                                                                                            ttATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTAcc                                                        <  2:307702/148‑1 (MQ=255)
                                                                                               tttACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACg                                                     <  1:3256939/148‑1 (MQ=255)
                                                                                               tttACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACg                                                     >  1:1422441/1‑148 (MQ=255)
                                                                                                 tACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGa                                                   >  1:1610231/1‑148 (MQ=255)
                                                                                                  aCATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGaa                                                  <  1:337454/148‑1 (MQ=255)
                                                                                                  aCATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGaa                                                  <  2:1733930/148‑1 (MQ=255)
                                                                                                      ccccATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGc                                              >  2:159004/1‑148 (MQ=255)
                                                                                                       cccATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCa                                             >  2:2230761/1‑148 (MQ=255)
                                                                                                         cATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTAGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAAc                                           >  1:2739282/1‑148 (MQ=255)
                                                                                                          aTAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACa                                          >  2:533595/1‑148 (MQ=255)
                                                                                                          aTAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACa                                          >  2:2377063/1‑148 (MQ=255)
                                                                                                          aTAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACa                                          <  2:2873292/148‑1 (MQ=255)
                                                                                                          aTAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTAGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACa                                          <  2:1799469/148‑1 (MQ=255)
                                                                                                           tAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACAt                                         >  2:2205769/1‑148 (MQ=255)
                                                                                                            aGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATg                                        >  2:1043691/1‑148 (MQ=255)
                                                                                                            aGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATg                                        <  2:2073538/148‑1 (MQ=255)
                                                                                                            aGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATg                                        >  2:1610699/1‑148 (MQ=255)
                                                                                                            aGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATg                                        >  2:641532/1‑148 (MQ=255)
                                                                                                            aGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATg                                        >  1:2633436/1‑148 (MQ=255)
                                                                                                            aGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATg                                        >  2:1091186/1‑148 (MQ=255)
                                                                                                             gATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCACCATGa                                       >  2:2190382/1‑148 (MQ=255)
                                                                                                             gATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGa                                       <  1:1326879/148‑1 (MQ=255)
                                                                                                              attattTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGTGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCACCATGaa                                      >  2:2527476/1‑148 (MQ=255)
                                                                                                              attattTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGac                                      >  2:2763172/1‑147 (MQ=255)
                                                                                                              attattTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGaa                                      <  1:2108731/148‑1 (MQ=255)
                                                                                                              attattTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGaa                                      >  2:2451890/1‑148 (MQ=255)
                                                                                                                 attTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACa                                   >  2:2668549/1‑148 (MQ=255)
                                                                                                                  tttGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAg                                  <  1:2862371/148‑1 (MQ=255)
                                                                                                                  tttGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAg                                  >  2:3488836/1‑148 (MQ=255)
                                                                                                                   ttGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGa                                 <  1:333569/148‑1 (MQ=255)
                                                                                                                   ttGCGTCAGCTCACAAATACGCTTTTTCCCTAGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGa                                 >  2:1590324/1‑148 (MQ=255)
                                                                                                                    tGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGaa                                <  2:2842412/148‑1 (MQ=255)
                                                                                                                    tGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGaa                                <  1:2314927/148‑1 (MQ=255)
                                                                                                                    tGCGTCAGCTCACAAATACGCTTTTTCCCTAGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGaa                                <  1:1819667/148‑1 (MQ=255)
                                                                                                                        tCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAAtt                            <  2:1792392/148‑1 (MQ=255)
                                                                                                                         cAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTa                           <  2:2674664/148‑1 (MQ=255)
                                                                                                                         cAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTa                           >  1:1496439/1‑148 (MQ=255)
                                                                                                                         cAGCTCACAAAGACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTa                           <  1:2428278/148‑1 (MQ=255)
                                                                                                                           gCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACg                         >  1:2223165/1‑148 (MQ=255)
                                                                                                                            cTCACAAATACGCTTTTTCCCTAGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACgg                        <  1:192409/148‑1 (MQ=255)
                                                                                                                               acaAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGAtt                     >  1:2504748/1‑148 (MQ=255)
                                                                                                                                 aaaTACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTAc                   >  2:1542432/1‑148 (MQ=255)
                                                                                                                                   aTACGCTTTTTCCCTAGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCt                 <  2:2834818/148‑1 (MQ=255)
                                                                                                                                    tACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTg                <  1:1723243/148‑1 (MQ=255)
                                                                                                                                    tACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTg                >  2:3518278/1‑148 (MQ=255)
                                                                                                                                     aCGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTgg               <  2:162774/148‑1 (MQ=255)
                                                                                                                                     aCGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTgg               >  1:1950069/1‑148 (MQ=255)
                                                                                                                                           tttCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGaaa         <  1:2668570/148‑1 (MQ=255)
                                                                                                                                            ttCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAAt        >  1:1277983/1‑148 (MQ=255)
                                                                                                                                              cccTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCt      <  2:3355474/148‑1 (MQ=255)
                                                                                                                                               ccTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTa     >  1:936497/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGG  >  CP009273/1364220‑1364513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A