Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,552,629 C→A 94.2% D216Y (GAT→TAT)  ddpD ← D,D‑dipeptide permease system, ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,552,6290CA94.2% 490.1 / 20.1 172D216Y (GAT→TAT) ddpDD,D‑dipeptide permease system, ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base C (2/8);  new base A (96/66);  total (98/74)
Fisher's exact test for biased strand distribution p-value = 2.03e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 173 total aligned reads displayed.

CCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGC  >  CP009273/1552484‑1552773
                                                                                                                                                 |                                                                                                                                                
ccATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAAt                                                                                                                                                <  2:325331/148‑1 (MQ=255)
  aTGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATgg                                                                                                                                              <  2:2137975/148‑1 (MQ=255)
  aTGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATgg                                                                                                                                              <  1:3102234/148‑1 (MQ=255)
  aTGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATgg                                                                                                                                              <  2:2736663/148‑1 (MQ=255)
  aTGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATgg                                                                                                                                              <  2:61347/148‑1 (MQ=255)
   tGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGc                                                                                                                                             >  2:645910/1‑148 (MQ=255)
    gTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCt                                                                                                                                            >  2:1448223/1‑148 (MQ=255)
         ggTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCtgatga                                                                                                                                       >  1:2564771/1‑148 (MQ=255)
         ggTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCtgatga                                                                                                                                       >  1:1817295/1‑148 (MQ=255)
         ggTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCtgatga                                                                                                                                       <  1:3168356/148‑1 (MQ=255)
         ggTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCtgatga                                                                                                                                       <  2:435928/148‑1 (MQ=255)
          gTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGaa                                                                                                                                      <  2:1812996/148‑1 (MQ=255)
          gTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGaa                                                                                                                                      <  2:1812992/148‑1 (MQ=255)
             cgcATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAg                                                                                                                                   <  1:96359/148‑1 (MQ=255)
               cATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGaa                                                                                                                                 <  2:344962/148‑1 (MQ=255)
               cATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGaa                                                                                                                                 <  2:2135214/148‑1 (MQ=255)
               cATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGaa                                                                                                                                 >  2:2375731/1‑148 (MQ=255)
                aTTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAAc                                                                                                                                >  2:1732001/1‑148 (MQ=255)
                aTTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAAc                                                                                                                                >  2:1731979/1‑148 (MQ=255)
                 ttGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAAcc                                                                                                                               >  2:2601851/1‑148 (MQ=255)
                  tGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCg                                                                                                                              >  2:830055/1‑148 (MQ=255)
                     agcaAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAg                                                                                                                           <  2:2801265/148‑1 (MQ=255)
                       caAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGtt                                                                                                                         >  2:2761811/1‑148 (MQ=255)
                        aaaCCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTc                                                                                                                        <  1:1518673/148‑1 (MQ=255)
                          aCCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCa                                                                                                                      <  2:814323/148‑1 (MQ=255)
                           ccAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCAc                                                                                                                     <  1:394888/148‑1 (MQ=255)
                             aaTGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTg                                                                                                                   >  2:1082742/1‑148 (MQ=255)
                             aaTGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTg                                                                                                                   >  2:1734272/1‑148 (MQ=255)
                             aaTGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTg                                                                                                                   >  2:2858804/1‑148 (MQ=255)
                              aTGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTgg                                                                                                                  >  1:705669/1‑148 (MQ=255)
                               tGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGgc                                                                                                                 >  1:2686982/1‑148 (MQ=255)
                                ggTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGgcg                                                                                                                >  1:2934834/1‑148 (MQ=255)
                                   ataCGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATAGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCgg                                                                                                             >  1:2732452/1‑148 (MQ=255)
                                    taCGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCggg                                                                                                            <  1:1976852/148‑1 (MQ=255)
                                      cGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCt                                                                                                          <  1:84732/148‑1 (MQ=255)
                                        gATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCttt                                                                                                        >  1:2179322/1‑148 (MQ=255)
                                         aTGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTa                                                                                                       >  2:2198727/1‑148 (MQ=255)
                                         aTGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTa                                                                                                       >  1:3212744/1‑148 (MQ=255)
                                              ggggATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGttt                                                                                                  <  1:26620/148‑1 (MQ=255)
                                              ggggATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGttt                                                                                                  <  1:26664/148‑1 (MQ=255)
                                               gggATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTa                                                                                                 >  2:3532673/1‑148 (MQ=255)
                                                 gatgatGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAg                                                                                               <  2:1579746/148‑1 (MQ=255)
                                                    gatgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCaa                                                                                            >  1:891876/1‑148 (MQ=255)
                                                    gatgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCGTTATGTTTAAGCaa                                                                                            >  1:649884/1‑148 (MQ=255)
                                                     atgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCaaa                                                                                           <  1:1913943/148‑1 (MQ=255)
                                                      tgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAAc                                                                                          >  1:1209618/1‑148 (MQ=255)
                                                        gATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGc                                                                                        >  1:3513783/1‑148 (MQ=255)
                                                        gATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGc                                                                                        >  2:857989/1‑148 (MQ=255)
                                                           aaCGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGt                                                                                     <  2:1214087/148‑1 (MQ=255)
                                                           aaCGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGt                                                                                     >  2:2460312/1‑148 (MQ=255)
                                                            aCGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTa                                                                                    <  1:1370020/148‑1 (MQ=255)
                                                             cGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTAc                                                                                   >  2:1106343/1‑148 (MQ=255)
                                                              gTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTAcc                                                                                  <  2:1684378/148‑1 (MQ=255)
                                                                  gCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCa                                                                              >  1:275514/1‑148 (MQ=255)
                                                                   ccGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCaa                                                                             <  1:1732001/148‑1 (MQ=255)
                                                                           ccgcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCg                                                                     >  1:2644266/1‑148 (MQ=255)
                                                                           ccgcTTTCAATTACGCTTCCGGCATACACCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCg                                                                     >  1:379654/1‑148 (MQ=255)
                                                                            cgcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGt                                                                    >  2:2125629/1‑148 (MQ=255)
                                                                             gcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTg                                                                   >  2:2330923/1‑148 (MQ=255)
                                                                              cTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGa                                                                  <  1:859205/148‑1 (MQ=255)
                                                                              cTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGa                                                                  <  2:398678/148‑1 (MQ=255)
                                                                              cTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGa                                                                  <  2:873261/148‑1 (MQ=255)
                                                                                ttCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACg                                                                >  2:784422/1‑148 (MQ=255)
                                                                                 tCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGt                                                               >  2:2234625/1‑148 (MQ=255)
                                                                                    aTTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTTCACCGTGACGTCCa                                                            >  1:2666121/1‑148 (MQ=255)
                                                                                    aTTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTTCACCGTGACGTCCa                                                            >  1:191026/1‑148 (MQ=255)
                                                                                    aTTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTTCACCGTGACGTCCa                                                            >  1:2666085/1‑148 (MQ=255)
                                                                                        cGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAgcgc                                                        <  2:420538/148‑1 (MQ=255)
                                                                                            tCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTa                                                    >  1:2607278/1‑148 (MQ=255)
                                                                                             ccGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAg                                                   <  2:680798/148‑1 (MQ=255)
                                                                                              cGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGt                                                  <  2:1141686/148‑1 (MQ=255)
                                                                                              cGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGt                                                  <  1:3345452/148‑1 (MQ=255)
                                                                                              cGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGt                                                  <  1:1649176/148‑1 (MQ=255)
                                                                                               ggCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTc                                                 <  2:676615/148‑1 (MQ=255)
                                                                                                  aTACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGt                                              >  2:1208314/1‑148 (MQ=255)
                                                                                                  aTACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGt                                              >  2:1208250/1‑148 (MQ=255)
                                                                                                    aCATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtc                                            >  2:1879477/1‑148 (MQ=255)
                                                                                                    aCATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtc                                            >  2:1879343/1‑148 (MQ=255)
                                                                                                    aCATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtc                                            >  1:659961/1‑148 (MQ=255)
                                                                                                      aTCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgt                                          <  1:2242604/148‑1 (MQ=255)
                                                                                                      aTCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATAGAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgt                                          <  2:3370840/148‑1 (MQ=255)
                                                                                                        cACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgtcg                                        >  2:3014466/1‑148 (MQ=255)
                                                                                                           gTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCa                                     <  2:1994538/148‑1 (MQ=255)
                                                                                                             aaaCGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaat                                   >  1:189486/1‑148 (MQ=255)
                                                                                                              aaCGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaata                                  >  1:2242645/1‑148 (MQ=255)
                                                                                                                  cTATCGCACAGTTGCGACACCACGGCCATATCTTGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCa                              <  2:151846/148‑1 (MQ=255)
                                                                                                                  cTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCa                              <  1:1448223/148‑1 (MQ=255)
                                                                                                                  cTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCa                              >  2:2029950/1‑148 (MQ=255)
                                                                                                                   tATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCaa                             <  1:557621/148‑1 (MQ=255)
                                                                                                                    aTCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAAt                            <  1:1344962/148‑1 (MQ=255)
                                                                                                                    aTCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTAGGCAATAATCAAt                            <  2:1491248/148‑1 (MQ=255)
                                                                                                                     tCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAAtt                           <  1:742129/148‑1 (MQ=255)
                                                                                                                           aGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCt                     >  1:1985081/1‑148 (MQ=255)
                                                                                                                           aGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCt                     >  2:916143/1‑148 (MQ=255)
                                                                                                                             ttGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCg                   <  1:2038675/148‑1 (MQ=255)
                                                                                                                             ttGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCg                   >  2:293994/1‑148 (MQ=255)
                                                                                                                              tGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGc                  <  1:1968368/148‑1 (MQ=255)
                                                                                                                                    accacGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGgaga            <  1:1763267/148‑1 (MQ=255)
                                                                                                                                             atatAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAgcg   >  1:223353/1‑148 (MQ=255)
                                                                                                                                              tataATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAgcgc  >  1:1014717/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGC  >  CP009273/1552484‑1552773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCA  >  CP009273/1552480‑1552778
                                                                                                                                                     |                                                                                                                                                     
TACTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATA                                                                                                                                                       >  SRR19973067.819907/1‑150 (MQ=60)
 ACTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAA                                                                                                                                                      >  SRR19973067.496232/1‑150 (MQ=60)
  CTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAAT                                                                                                                                                     >  SRR19973067.41357/1‑150 (MQ=60)
   TCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATG                                                                                                                                                    <  SRR19973067.3288589/150‑1 (MQ=60)
   TCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGACCATATAATG                                                                                                                                                    <  SRR19973067.2168432/150‑1 (MQ=60)
   TCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGACCATATAATG                                                                                                                                                    <  SRR19973067.2168464/150‑1 (MQ=60)
     CATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGC                                                                                                                                                  >  SRR19973067.1590561/1‑150 (MQ=60)
     CATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGC                                                                                                                                                  >  SRR19973067.3078705/1‑150 (MQ=60)
      ATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCT                                                                                                                                                 <  SRR19973067.3121969/150‑1 (MQ=60)
           CTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGA                                                                                                                                            >  SRR19973067.2581523/1‑150 (MQ=60)
           CTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGA                                                                                                                                            >  SRR19973067.1829597/1‑150 (MQ=60)
             GGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAAC                                                                                                                                          <  SRR19973067.3188416/150‑1 (MQ=60)
                 CGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAA                                                                                                                                      <  SRR19973067.97359/150‑1 (MQ=60)
                           CAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCC                                                                                                                            >  SRR19973067.3317672/1‑150 (MQ=60)
                           CAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCC                                                                                                                            >  SRR19973067.2720306/1‑150 (MQ=60)
                            AAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCA                                                                                                                           <  SRR19973067.1528825/150‑1 (MQ=60)
                             AACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCAC                                                                                                                          >  SRR19973067.939760/1‑150 (MQ=60)
                               CCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTG                                                                                                                        <  SRR19973067.397644/150‑1 (MQ=60)
                                CAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGG                                                                                                                       >  SRR19973067.1940544/1‑150 (MQ=60)
                                CAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGG                                                                                                                       >  SRR19973067.710550/1‑150 (MQ=60)
                                 AATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGC                                                                                                                      >  SRR19973067.2704225/1‑150 (MQ=60)
                                 AATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGC                                                                                                                      >  SRR19973067.974649/1‑150 (MQ=60)
                                  ATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCG                                                                                                                     >  SRR19973067.2953456/1‑150 (MQ=60)
                                     GTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATAGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGG                                                                                                                  >  SRR19973067.2750088/1‑150 (MQ=60)
                                      TATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGG                                                                                                                 <  SRR19973067.689763/150‑1 (MQ=60)
                                        TACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCT                                                                                                               <  SRR19973067.1990238/150‑1 (MQ=60)
                                        TACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCT                                                                                                               <  SRR19973067.1990244/150‑1 (MQ=60)
                                          CGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACCTCACGGAAACGCTCTCGCACAGTTGCGACACCCCGGCCCTATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTgg                                                                                                             >  SRR19973067.1716109/1‑148 (MQ=60)
                                          CGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTT                                                                                                             <  SRR19973067.85655/150‑1 (MQ=60)
                                          CGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTT                                                                                                             >  SRR19973067.2193896/1‑150 (MQ=60)
                                           GGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTA                                                                                                            >  SRR19973067.3232975/1‑150 (MQ=60)
                                                  GGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAA                                                                                                     <  SRR19973067.27081/150‑1 (MQ=60)
                                                  GGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAA                                                                                                     <  SRR19973067.27037/150‑1 (MQ=60)
                                                      ATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAA                                                                                                 >  SRR19973067.2688497/1‑150 (MQ=60)
                                                      ATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAA                                                                                                 >  SRR19973067.653900/1‑150 (MQ=60)
                                                      ATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAA                                                                                                 >  SRR19973067.897777/1‑150 (MQ=60)
                                                      ATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCGTTATGTTTAAGCAA                                                                                                 >  SRR19973067.654292/1‑150 (MQ=60)
                                                        GATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACCGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAAC                                                                                               >  SRR19973067.1217582/1‑150 (MQ=60)
                                                         ATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACG                                                                                              <  SRR19973067.1927006/150‑1 (MQ=60)
                                                          TNGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGC                                                                                             >  SRR19973067.3536031/1‑150 (MQ=60)
                                                          TGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGC                                                                                             >  SRR19973067.3531582/1‑150 (MQ=60)
                                                          TGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGC                                                                                             >  SRR19973067.1461573/1‑150 (MQ=60)
                                                             ATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGT                                                                                          <  SRR19973067.1797037/150‑1 (MQ=60)
                                                              TAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTA                                                                                         <  SRR19973067.775150/150‑1 (MQ=60)
                                                              TAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTA                                                                                         >  SRR19973067.1864022/1‑150 (MQ=60)
                                                                ACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACC                                                                                       <  SRR19973067.1379377/150‑1 (MQ=60)
                                                                   TCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGC                                                                                    >  SRR19973067.2662262/1‑150 (MQ=60)
                                                                    CTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCA                                                                                   >  SRR19973067.277338/1‑150 (MQ=60)
                                                                       CCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACT                                                                                <  SRR19973067.1743843/150‑1 (MQ=60)
                                                                       CCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACT                                                                                <  SRR19973067.1743865/150‑1 (MQ=60)
                                                                             CGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCG                                                                          >  SRR19973067.2661344/1‑150 (MQ=60)
                                                                             CGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTACACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCG                                                                          >  SRR19973067.381694/1‑150 (MQ=60)
                                                                             CGCCGCTTTCAATTACGCTTCCGGCATACACCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCG                                                                          >  SRR19973067.382297/1‑150 (MQ=60)
                                                                                  CTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACG                                                                     <  SRR19973067.864979/150‑1 (MQ=60)
                                                                                  CTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACG                                                                     <  SRR19973067.2083780/150‑1 (MQ=60)
                                                                                      CAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTTCACCGTGACGTCCA                                                                 >  SRR19973067.2683214/1‑150 (MQ=60)
                                                                                      CAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTTCACCGTGACGTCCA                                                                 >  SRR19973067.2683250/1‑150 (MQ=60)
                                                                                      CAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTTCACCGTGACGTCCA                                                                 >  SRR19973067.192504/1‑150 (MQ=60)
                                                                                      CAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTTCACCGTGACGTCCA                                                                 >  SRR19973067.2683450/1‑150 (MQ=60)
                                                                                              CTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTA                                                         >  SRR19973067.2624216/1‑150 (MQ=60)
                                                                                              CTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTA                                                         >  SRR19973067.2009698/1‑150 (MQ=60)
                                                                                                  CGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCG                                                     <  SRR19973067.3366649/150‑1 (MQ=60)
                                                                                                  CGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCG                                                     <  SRR19973067.1660534/150‑1 (MQ=60)
                                                                                                      ATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTC                                                 >  SRR19973067.664451/1‑150 (MQ=60)
                                                                                                      ATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTC                                                 >  SRR19973067.888697/1‑150 (MQ=60)
                                                                                                         CATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTC                                              >  SRR19973067.1920179/1‑150 (MQ=60)
                                                                                                          ATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCG                                             <  SRR19973067.2257509/150‑1 (MQ=60)
                                                                                                               GTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAAT                                        >  SRR19973067.190958/1‑150 (MQ=60)
                                                                                                               GTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAAT                                        >  SRR19973067.283261/1‑150 (MQ=60)
                                                                                                                TAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATA                                       >  SRR19973067.2257550/1‑150 (MQ=60)
                                                                                                                TAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATA                                       >  SRR19973067.2257513/1‑150 (MQ=60)
                                                                                                                    CGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCA                                   >  SRR19973067.2681321/1‑150 (MQ=60)
                                                                                                                      CTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAAT                                 <  SRR19973067.1457935/150‑1 (MQ=60)
                                                                                                                      CTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAAT                                 <  SRR19973067.717013/150‑1 (MQ=60)
                                                                                                                       TATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATT                                <  SRR19973067.561275/150‑1 (MQ=60)
                                                                                                                        ATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTG                               <  SRR19973067.1355151/150‑1 (MQ=60)
                                                                                                                        ATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTG                               <  SRR19973067.1350874/150‑1 (MQ=60)
                                                                                                                        ATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTG                               <  SRR19973067.1354164/150‑1 (MQ=60)
                                                                                                                         TCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGC                              <  SRR19973067.747273/150‑1 (MQ=60)
                                                                                                                         TCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGC                              <  SRR19973067.2542378/150‑1 (MQ=60)
                                                                                                                           GCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGG                            <  SRR19973067.2618770/150‑1 (MQ=60)
                                                                                                                             ACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCT                          >  SRR19973067.1998515/1‑150 (MQ=60)
                                                                                                                                 TTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCA                      <  SRR19973067.2052316/150‑1 (MQ=60)
                                                                                                                                  TGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAG                     <  SRR19973067.1981723/150‑1 (MQ=60)
                                                                                                                                     GACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAG                  <  SRR19973067.3150064/150‑1 (MQ=60)
                                                                                                                                        ACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAAT               <  SRR19973067.1775262/150‑1 (MQ=60)
                                                                                                                                               CCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCG        >  SRR19973067.224979/1‑150 (MQ=60)
                                                                                                                                               CCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGATCGTCGGCAATAATCAATTGAGGCTCGCAGGAGAATTCCAGCG        >  SRR19973067.224951/1‑150 (MQ=60)
                                                                                                                                                CATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGC       >  SRR19973067.1021683/1‑150 (MQ=60)
                                                                                                                                                 ATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCA      >  SRR19973067.3450769/1‑150 (MQ=60)
                                                                                                                                                 ATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCA      >  SRR19973067.3450530/1‑150 (MQ=60)
                                                                                                                                                   ATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAAT    <  SRR19973067.1740253/150‑1 (MQ=60)
                                                                                                                                                   ATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAAT    <  SRR19973067.3198071/150‑1 (MQ=60)
                                                                                                                                                   ATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAAT    <  SRR19973067.3198059/150‑1 (MQ=60)
                                                                                                                                                   ATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAAT    <  SRR19973067.1113856/150‑1 (MQ=60)
                                                                                                                                                    TAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATC   <  SRR19973067.2122770/150‑1 (MQ=60)
                                                                                                                                                    TAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATC   <  SRR19973067.2122822/150‑1 (MQ=60)
                                                                                                                                                     AATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCA  >  SRR19973067.762171/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TACTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCA  >  CP009273/1552480‑1552778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑