Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,322,609 C→A 100% W223L (TGG→TTG)  yfaQ ← tandem DUF2300 domain protein, putative host defense protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,322,6090CA97.2% 544.2 / 6.2 176W223L (TGG→TTG) yfaQtandem DUF2300 domain protein, putative host defense protein
Reads supporting (aligned to +/- strand):  ref base C (0/5);  new base A (70/101);  total (70/106)
Fisher's exact test for biased strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 182 total aligned reads displayed.

TGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCA  >  CP009273/2322464‑2322753
                                                                                                                                                 |                                                                                                                                                
tgtgATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCaac                                                                                                                                                >  2:1531318/1‑148 (MQ=255)
tgtgATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCaac                                                                                                                                                <  2:2346058/148‑1 (MQ=255)
tgtgATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCaac                                                                                                                                                >  2:263241/1‑148 (MQ=255)
  tgATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAg                                                                                                                                              >  1:3328391/1‑148 (MQ=255)
  tgATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAg                                                                                                                                              >  1:3109553/1‑148 (MQ=255)
  tgATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACNGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAg                                                                                                                                              >  1:1803501/1‑148 (MQ=255)
       ttcttcAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGgtg                                                                                                                                         <  2:1891091/148‑1 (MQ=255)
                   tAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTa                                                                                                                             >  1:2952353/1‑148 (MQ=255)
                         gCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAccagc                                                                                                                       >  1:2208900/1‑148 (MQ=255)
                         gCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAccagc                                                                                                                       >  1:2208736/1‑148 (MQ=255)
                          cAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAccagcc                                                                                                                      >  2:1337030/1‑148 (MQ=255)
                          cAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAccagcc                                                                                                                      <  2:1782102/148‑1 (MQ=255)
                                gCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCCCTGCCATTAACCAACAGCGCTGTACCATCGGTTAACCAGCCAGCaaa                                                                                                                >  2:580573/1‑148 (MQ=255)
                                gCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCaaa                                                                                                                >  2:2377076/1‑148 (MQ=255)
                                 cTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCaaaa                                                                                                               >  1:3309930/1‑148 (MQ=255)
                                 cTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCaaaa                                                                                                               <  1:628164/148‑1 (MQ=255)
                                      ccTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAaccacc                                                                                                          <  1:1938390/148‑1 (MQ=255)
                                      ccTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAaccacc                                                                                                          <  1:1828815/148‑1 (MQ=255)
                                      ccTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAaccacc                                                                                                          >  2:2958347/1‑148 (MQ=255)
                                            cacaTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACg                                                                                                    <  2:1933696/148‑1 (MQ=255)
                                             acaTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACgg                                                                                                   <  1:420134/148‑1 (MQ=255)
                                              caTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGa                                                                                                  >  1:3192705/1‑148 (MQ=255)
                                                ttGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGTTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGAtt                                                                                                >  2:1759955/1‑148 (MQ=255)
                                                ttGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGAtg                                                                                                >  2:1058682/1‑147 (MQ=255)
                                                 tGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGAttt                                                                                               <  2:357533/148‑1 (MQ=255)
                                                  gCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTc                                                                                              <  2:1911195/148‑1 (MQ=255)
                                                  gCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTc                                                                                              <  1:537934/148‑1 (MQ=255)
                                                     cACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTg                                                                                           <  1:2048789/148‑1 (MQ=255)
                                                      aCTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGt                                                                                          <  1:263256/148‑1 (MQ=255)
                                                      aCTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGt                                                                                          <  1:2682085/148‑1 (MQ=255)
                                                       cTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGtt                                                                                         <  1:51236/148‑1 (MQ=255)
                                                        tGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGttt                                                                                        <  1:97094/148‑1 (MQ=255)
                                                        tGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGttt                                                                                        >  1:1205132/1‑148 (MQ=255)
                                                         ggCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTa                                                                                       <  2:3481031/148‑1 (MQ=255)
                                                         ggCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTa                                                                                       <  2:2511164/148‑1 (MQ=255)
                                                         ggCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTa                                                                                       <  2:3480867/148‑1 (MQ=255)
                                                          gCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTat                                                                                      <  2:336620/148‑1 (MQ=255)
                                                           cAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTatc                                                                                     >  1:1543677/1‑148 (MQ=255)
                                                            aaCCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTatca                                                                                    <  2:1852876/148‑1 (MQ=255)
                                                             aCCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTatcat                                                                                   >  2:571851/1‑148 (MQ=255)
                                                             aCCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTatcat                                                                                   <  2:893212/148‑1 (MQ=255)
                                                              ccTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTatcatc                                                                                  <  1:1390085/148‑1 (MQ=255)
                                                              ccTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTatcatc                                                                                  <  2:3328382/148‑1 (MQ=255)
                                                              ccTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTatcatc                                                                                  <  2:3109553/148‑1 (MQ=255)
                                                                       gCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCa                                                                         >  2:1851951/1‑148 (MQ=255)
                                                                        cACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAg                                                                        <  1:2677191/148‑1 (MQ=255)
                                                                         aCTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCTGCAAACCAGc                                                                       <  2:2411779/148‑1 (MQ=255)
                                                                           tGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTc                                                                     <  1:3370266/148‑1 (MQ=255)
                                                                           tGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTc                                                                     >  1:2925103/1‑148 (MQ=255)
                                                                              cAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAg                                                                  <  1:3514649/148‑1 (MQ=255)
                                                                                 aaCCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGtt                                                               >  1:741165/1‑148 (MQ=255)
                                                                                 aaCCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGtt                                                               <  2:2887708/148‑1 (MQ=255)
                                                                                 aaCCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGtt                                                               <  2:1421763/148‑1 (MQ=255)
                                                                                   cccGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGtttt                                                             <  1:1012191/148‑1 (MQ=255)
                                                                                   cccGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGtttt                                                             <  1:1010146/148‑1 (MQ=255)
                                                                                    ccGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTc                                                            <  2:1383295/148‑1 (MQ=255)
                                                                                    ccGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTc                                                            <  2:3133493/148‑1 (MQ=255)
                                                                                      gATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCAc                                                          >  2:1713091/1‑148 (MQ=255)
                                                                                      gATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCAc                                                          <  2:2894928/148‑1 (MQ=255)
                                                                                       aTTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCAcc                                                         <  2:829421/148‑1 (MQ=255)
                                                                                        ttAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCAccc                                                        <  1:3343985/148‑1 (MQ=255)
                                                                                         tAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCg                                                       >  1:2033731/1‑148 (MQ=255)
                                                                                          aaGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGt                                                      <  2:1331902/148‑1 (MQ=255)
                                                                                           aGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTa                                                     <  2:2263032/148‑1 (MQ=255)
                                                                                             cACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTAcc                                                   <  2:741165/148‑1 (MQ=255)
                                                                                             cACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTAcc                                                   <  1:66292/148‑1 (MQ=255)
                                                                                             cACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTAcc                                                   <  1:2446366/148‑1 (MQ=255)
                                                                                             cACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTAcc                                                   >  1:3226927/1‑148 (MQ=255)
                                                                                                 gTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGAc                                               <  2:954953/148‑1 (MQ=255)
                                                                                                 gTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGAc                                               <  1:3373922/148‑1 (MQ=255)
                                                                                                  tCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACt                                              <  2:2640902/148‑1 (MQ=255)
                                                                                                   cGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTa                                             >  1:1641080/1‑148 (MQ=255)
                                                                                                   cGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTa                                             >  1:3030180/1‑148 (MQ=255)
                                                                                                   cGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTa                                             >  2:3437161/1‑148 (MQ=255)
                                                                                                   cGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTa                                             <  2:3181124/148‑1 (MQ=255)
                                                                                                      aTAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCg                                          <  1:1365829/148‑1 (MQ=255)
                                                                                                       tAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGa                                         >  2:476598/1‑148 (MQ=255)
                                                                                                        aaCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAg                                        <  1:665634/148‑1 (MQ=255)
                                                                                                        aaCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAg                                        <  1:2836902/148‑1 (MQ=255)
                                                                                                        aaCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAg                                        >  1:1124723/1‑148 (MQ=255)
                                                                                                         aCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGt                                                                <  1:2162964/123‑1 (MQ=255)
                                                                                                         aCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGt                                                                >  2:2162964/1‑123 (MQ=255)
                                                                                                         aCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGc                                       <  2:1995811/148‑1 (MQ=255)
                                                                                                         aCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGc                                       <  2:1996007/148‑1 (MQ=255)
                                                                                                         aCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGc                                       <  1:1205552/148‑1 (MQ=255)
                                                                                                            ggTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACTCGACTACCGAGCATg                                    >  1:1368756/1‑148 (MQ=255)
                                                                                                            ggTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATg                                    <  1:2495063/148‑1 (MQ=255)
                                                                                                             gTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATgg                                   >  1:732301/1‑148 (MQ=255)
                                                                                                             gTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATgg                                   <  2:3244795/148‑1 (MQ=255)
                                                                                                                   aCCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGa                             <  1:2536091/148‑1 (MQ=255)
                                                                                                                     cGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGaca                           <  1:3553093/148‑1 (MQ=255)
                                                                                                                             tGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAGGCCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGAtttt                   >  2:295457/1‑148 (MQ=255)
                                                                                                                             tGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATGTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGAtttt                   >  2:2120606/1‑148 (MQ=255)
                                                                                                                                tGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGc                <  1:402090/148‑1 (MQ=255)
                                                                                                                                 gCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCTATTTTCGcc               <  1:2377076/148‑1 (MQ=255)
                                                                                                                                    aCTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCg            >  2:1152351/1‑148 (MQ=255)
                                                                                                                                      tGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTc          <  2:422877/148‑1 (MQ=255)
                                                                                                                                            tAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGcac    >  2:555789/1‑148 (MQ=255)
                                                                                                                                            tAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGcac    <  1:3510025/148‑1 (MQ=255)
                                                                                                                                              aCCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGcacca  >  2:1067655/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCA  >  CP009273/2322464‑2322753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCC  >  CP009273/2322463‑2322758
                                                                                                                                                  |                                                                                                                                                     
CTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACA                                                                                                                                                    <  SRR19973067.2266847/150‑1 (MQ=60)
CTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACA                                                                                                                                                    >  SRR19973067.1671544/1‑150 (MQ=60)
 TGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAG                                                                                                                                                   >  SRR19973067.3349437/1‑150 (MQ=60)
 TGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAG                                                                                                                                                   >  SRR19973067.3349446/1‑150 (MQ=60)
 TGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAG                                                                                                                                                   >  SRR19973067.3129333/1‑150 (MQ=60)
 TGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACNGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAG                                                                                                                                                   >  SRR19973067.1815640/1‑150 (MQ=60)
      TTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTG                                                                                                                                              <  SRR19973067.3173703/150‑1 (MQ=60)
         TCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTAC                                                                                                                                           >  SRR19973067.2390062/1‑150 (MQ=60)
          CTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACC                                                                                                                                          >  SRR19973067.3114750/1‑150 (MQ=60)
           TTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCA                                                                                                                                         <  SRR19973067.3315661/150‑1 (MQ=60)
                 GGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTT                                                                                                                                   >  SRR19973067.2949761/1‑150 (MQ=60)
                  GATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTA                                                                                                                                  >  SRR19973067.2971027/1‑150 (MQ=60)
                     AGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACC                                                                                                                               <  SRR19973067.2061104/150‑1 (MQ=60)
                        GGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGC                                                                                                                            >  SRR19973067.2223628/1‑150 (MQ=60)
                        GGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGC                                                                                                                            >  SRR19973067.2223462/1‑150 (MQ=60)
                               CAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAA                                                                                                                     >  SRR19973067.2917113/1‑150 (MQ=60)
                                AGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAA                                                                                                                    >  SRR19973067.3330829/1‑150 (MQ=60)
                                  CTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACC                                                                                                                  <  SRR19973067.632347/150‑1 (MQ=60)
                                      ACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCC                                                                                                              <  SRR19973067.2445740/150‑1 (MQ=60)
                                       CCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCT                                                                                                             <  SRR19973067.1951608/150‑1 (MQ=60)
                                       CCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCT                                                                                                             <  SRR19973067.1841245/150‑1 (MQ=60)
                                             CACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGA                                                                                                       >  SRR19973067.3212865/1‑150 (MQ=60)
                                              ACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGAT                                                                                                      <  SRR19973067.423057/150‑1 (MQ=60)
                                                ATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTT                                                                                                    >  SRR19973067.865631/1‑150 (MQ=60)
                                                  TGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTNC                                                                                                  <  SRR19973067.3487126/150‑1 (MQ=60)
                                                   GCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCT                                                                                                 <  SRR19973067.541525/150‑1 (MQ=60)
                                                    CCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG                                                                                                >  SRR19973067.1383552/1‑150 (MQ=60)
                                                      CACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTT                                                                                              <  SRR19973067.2062471/150‑1 (MQ=60)
                                                      CACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCAAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTT                                                                                              <  SRR19973067.2108715/150‑1 (MQ=60)
                                                       ACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTT                                                                                             >  SRR19973067.1213023/1‑150 (MQ=60)
                                                       ACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTT                                                                                             <  SRR19973067.265028/150‑1 (MQ=60)
                                                       ACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTT                                                                                             <  SRR19973067.265043/150‑1 (MQ=60)
                                                       ACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTT                                                                                             <  SRR19973067.2699265/150‑1 (MQ=60)
                                                       ACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTT                                                                                             <  SRR19973067.2699272/150‑1 (MQ=60)
                                                        CTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTA                                                                                            <  SRR19973067.51899/150‑1 (MQ=60)
                                                         TGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTAT                                                                                           <  SRR19973067.98916/150‑1 (MQ=60)
                                                         TGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTAT                                                                                           <  SRR19973067.98098/150‑1 (MQ=60)
                                                          GGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATC                                                                                          >  SRR19973067.1554148/1‑150 (MQ=60)
                                                             AACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATC                                                                                       <  SRR19973067.533247/150‑1 (MQ=60)
                                                               CCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGG                                                                                     <  SRR19973067.1399532/150‑1 (MQ=60)
                                                                 TGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACACTGACGGATTTCCTGTTTATCATCGGCA                                                                                   <  SRR19973067.1641839/150‑1 (MQ=60)
                                                                         CACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCT                                                                           <  SRR19973067.2694357/150‑1 (MQ=60)
                                                                          ACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTC                                                                          >  SRR19973067.2943691/1‑150 (MQ=60)
                                                                           CTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCC                                                                         <  SRR19973067.2485648/150‑1 (MQ=60)
                                                                            TGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCA                                                                        <  SRR19973067.3391677/150‑1 (MQ=60)
                                                                            TGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCA                                                                        <  SRR19973067.3391900/150‑1 (MQ=60)
                                                                             GGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAG                                                                       >  SRR19973067.2517064/1‑150 (MQ=60)
                                                                               CAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGT                                                                     <  SRR19973067.3536899/150‑1 (MQ=60)
                                                                                AAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTT                                                                    >  SRR19973067.746302/1‑150 (MQ=60)
                                                                                 tcagacgtgtgctcttccgatctTAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTT                                                                   <  SRR19973067.2177442/127‑1 (MQ=60)
                                                                                 tcagacgtgtgctcttccgatctTAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTT                                                                   <  SRR19973067.2177422/127‑1 (MQ=60)
                                                                                 AAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTT                                                                   <  SRR19973067.2773731/150‑1 (MQ=60)
                                                                                 AAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTT                                                                   <  SRR19973067.200397/150‑1 (MQ=60)
                                                                                   ACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTC                                                                 >  SRR19973067.1243599/1‑150 (MQ=60)
                                                                                    CCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCA                                                                <  SRR19973067.1019142/150‑1 (MQ=60)
                                                                                    CCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCA                                                                <  SRR19973067.1017085/150‑1 (MQ=60)
                                                                                    CCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCA                                                                <  SRR19973067.3245963/150‑1 (MQ=60)
                                                                                       GATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCC                                                             >  SRR19973067.425819/1‑150 (MQ=60)
                                                                                        ATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCG                                                            >  SRR19973067.2047355/1‑150 (MQ=60)
                                                                                         TTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGT                                                           >  SRR19973067.744297/1‑150 (MQ=60)
                                                                                         TTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGT                                                           <  SRR19973067.3365174/150‑1 (MQ=60)
                                                                                            AGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACC                                                        >  SRR19973067.3247192/1‑150 (MQ=60)
                                                                                             GCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCC                                                       <  SRR19973067.1839259/150‑1 (MQ=60)
                                                                                              CACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCG                                                      <  SRR19973067.2462437/150‑1 (MQ=60)
                                                                                              CACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCG                                                      <  SRR19973067.67127/150‑1 (MQ=60)
                                                                                              CACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCG                                                      <  SRR19973067.67079/150‑1 (MQ=60)
                                                                                                  GTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTA                                                  >  SRR19973067.1652381/1‑150 (MQ=60)
                                                                                                  GTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTA                                                  <  SRR19973067.3395368/150‑1 (MQ=60)
                                                                                                  GTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTA                                                  >  SRR19973067.3049474/1‑150 (MQ=60)
                                                                                                       ATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAG                                             >  SRR19973067.1132321/1‑150 (MQ=60)
                                                                                                       ATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAG                                             <  SRR19973067.1375159/150‑1 (MQ=60)
                                                                                                         AACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCA                                           <  SRR19973067.670182/150‑1 (MQ=60)
                                                                                                         AACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCA                                           <  SRR19973067.2855129/150‑1 (MQ=60)
                                                                                                          ACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCAT                                          <  SRR19973067.1213379/150‑1 (MQ=60)
                                                                                                          ACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCAT                                          <  SRR19973067.1213449/150‑1 (MQ=60)
                                                                                                           CGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACTCGACTACCGAGCATG                                         >  SRR19973067.1378102/1‑150 (MQ=60)
                                                                                                            GGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGG                                        >  SRR19973067.737389/1‑150 (MQ=60)
                                                                                                             GGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGA                                       <  SRR19973067.2511453/150‑1 (MQ=60)
                                                                                                              GTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGAT                                      <  SRR19973067.1161865/150‑1 (MQ=60)
                                                                                                                 AGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCG                                   <  SRR19973067.569948/150‑1 (MQ=60)
                                                                                                                    ACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACA                                <  SRR19973067.2552699/150‑1 (MQ=60)
                                                                                                                      CGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACC                              <  SRR19973067.3575494/150‑1 (MQ=60)
                                                                                                                      CGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACC                              >  SRR19973067.705738/1‑150 (MQ=60)
                                                                                                                      CGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACC                              <  SRR19973067.3575511/150‑1 (MQ=60)
                                                                                                                           TGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTT                         <  SRR19973067.1407922/150‑1 (MQ=60)
                                                                                                                                 TGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCT                   <  SRR19973067.408411/150‑1 (MQ=60)
                                                                                                                                 TGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCT                   <  SRR19973067.404898/150‑1 (MQ=60)
                                                                                                                                  GCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCTATTTTCGCCTC                  <  SRR19973067.2392522/150‑1 (MQ=60)
                                                                                                                                   CCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCG                 <  SRR19973067.1041761/150‑1 (MQ=60)
                                                                                                                                    CACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGT                >  SRR19973067.3536156/1‑150 (MQ=60)
                                                                                                                                      CTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCC              >  SRR19973067.3387098/1‑150 (MQ=60)
                                                                                                                                             TAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCA       <  SRR19973067.3532258/150‑1 (MQ=60)
                                                                                                                                                  AACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCC  <  SRR19973067.1491681/150‑1 (MQ=60)
                                                                                                                                                  AACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCC  <  SRR19973067.1491687/150‑1 (MQ=60)
                                                                                                                                                  AACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCC  <  SRR19973067.2899286/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
CTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCC  >  CP009273/2322463‑2322758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑