Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,317,805 C→A 34.8% D156Y (GAT→TAT)  folP ← 7,8‑dihydropteroate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,317,8050CA34.8% 336.1 / 190.1 221D156Y (GAT→TAT) folP7,8‑dihydropteroate synthase
Reads supporting (aligned to +/- strand):  ref base C (71/73);  new base A (28/49);  total (99/122)
Fisher's exact test for biased strand distribution p-value = 8.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

Only 100 of 223 total aligned reads displayed.

CCAGGCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTT  >  CP009273/3317658‑3317949
                                                                                                                                                   |                                                                                                                                                
ccAGGCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATc                                                                                                                                                  >  2:149915/1‑148 (MQ=255)
  aGGCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGt                                                                                                                                                >  1:1016380/1‑148 (MQ=255)
  aGGCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGt                                                                                                                                                >  1:2791373/1‑148 (MQ=255)
   ggCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTc                                                                                                                                               <  2:2470308/148‑1 (MQ=255)
   ggCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTc                                                                                                                                               <  1:2105453/148‑1 (MQ=255)
    gcgcgcCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCa                                                                                                                                              <  1:2103625/148‑1 (MQ=255)
      gcgcCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATa                                                                                                                                            <  1:622629/148‑1 (MQ=255)
      gcgcCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATa                                                                                                                                            <  2:1083839/148‑1 (MQ=255)
       cgcCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAAACGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGAAGCGATTCACTTCTGCAAAGACATCGTCATAc                                                                                                                                           >  1:2852558/1‑148 (MQ=255)
          cAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTc                                                                                                                                        >  2:1591053/1‑148 (MQ=255)
           aGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCg                                                                                                                                       >  2:2406826/1‑148 (MQ=255)
           aGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCg                                                                                                                                       >  1:1955203/1‑148 (MQ=255)
            gTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTctg                                                                                                                                      >  1:277377/1‑146 (MQ=255)
            ctactGAATAGCTACGGGAGACAATTGTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCgg                                                                                                                                      <  2:2864270/144‑1 (MQ=255)
              aaTGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAg                                                                                                                                    >  1:1146081/1‑148 (MQ=255)
              aaTGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAg                                                                                                                                    >  2:843144/1‑148 (MQ=255)
               aTGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGc                                                                                                                                   >  1:1517144/1‑148 (MQ=255)
                 gAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCtt                                                                                                                                 >  1:456183/1‑148 (MQ=255)
                  aaTAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTc                                                                                                                                <  1:2952635/148‑1 (MQ=255)
                  aaTAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTc                                                                                                                                <  1:3109695/148‑1 (MQ=255)
                    tAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCt                                                                                                                              >  1:358209/1‑148 (MQ=255)
                        tATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCAt                                                                                                                          >  2:2868893/1‑148 (MQ=255)
                        tATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCAt                                                                                                                          >  2:473754/1‑148 (MQ=255)
                        tATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCAt                                                                                                                          >  2:2808678/1‑148 (MQ=255)
                         aTGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATg                                                                                                                         >  2:1488213/1‑148 (MQ=255)
                           gggAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGt                                                                                                                       <  2:1458690/148‑1 (MQ=255)
                             gagagaTTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGttt                                                                                                                     <  1:1206100/148‑1 (MQ=255)
                             gagagaTTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGttt                                                                                                                     >  2:1549054/1‑148 (MQ=255)
                                 gaTTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTgg                                                                                                                 <  1:344405/148‑1 (MQ=255)
                                 gaTTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTgg                                                                                                                 <  1:3195959/148‑1 (MQ=255)
                                  aTTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGa                                                                                                                <  1:1985497/148‑1 (MQ=255)
                                    ttttACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGAtt                                                                                                              >  1:475665/1‑148 (MQ=255)
                                        accgaaaccgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTccc                                                                                                          <  2:1594728/148‑1 (MQ=255)
                                            aaaccgagTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCa                                                                                                      <  2:1342468/148‑1 (MQ=255)
                                            aaaccgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCa                                                                                                      >  2:1051590/1‑148 (MQ=255)
                                              accgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCata                                                                                                    <  1:3159958/148‑1 (MQ=255)
                                              accgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCata                                                                                                    <  2:2676501/148‑1 (MQ=255)
                                               ccgaaGCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCatat                                                                                                   <  2:2241907/148‑1 (MQ=255)
                                                   aTCCGGGGTCGAGCAACAATTTCTCTTTTTCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCAt                                                                                               >  1:2749637/1‑148 (MQ=255)
                                                   aTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCAt                                                                                               >  2:591385/1‑148 (MQ=255)
                                                   aTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCAt                                                                                               <  1:1465570/148‑1 (MQ=255)
                                                   aTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCAt                                                                                               <  2:1685268/148‑1 (MQ=255)
                                                    tCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATc                                                                                              >  1:2285394/1‑148 (MQ=255)
                                                    tCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATc                                                                                              <  2:2444769/148‑1 (MQ=255)
                                                          gTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaa                                                                                        >  2:2982458/1‑148 (MQ=255)
                                                             gAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaacc                                                                                     >  1:228531/1‑148 (MQ=255)
                                                               gCAACAATTTCTCTTTTGCGAGACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccgg                                                                                   <  2:1769341/148‑1 (MQ=255)
                                                                caacaaTTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccggt                                                                                  >  2:3497484/1‑148 (MQ=255)
                                                                caacaaTTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccggt                                                                                  <  1:690852/148‑1 (MQ=255)
                                                                   caaTTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccggtaaa                                                                               <  1:1591053/148‑1 (MQ=255)
                                                                        tctcTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccggtaaaccggt                                                                          <  1:1753049/148‑1 (MQ=255)
                                                                          tctTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGttt                                                                        <  2:677582/148‑1 (MQ=255)
                                                                          tctTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGttt                                                                        <  2:3550144/148‑1 (MQ=255)
                                                                           ctTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTc                                                                       <  2:2584387/148‑1 (MQ=255)
                                                                           ctTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTc                                                                       <  2:2585396/148‑1 (MQ=255)
                                                                              ttGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCNTACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGc                                                                    >  1:1802661/1‑148 (MQ=255)
                                                                                    tACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAgccgc                                                              >  2:235815/1‑148 (MQ=255)
                                                                                     aCCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAgccgcc                                                             >  1:3222785/1‑148 (MQ=255)
                                                                                       ccGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTc                                                           >  2:3238593/1‑148 (MQ=255)
                                                                                         gCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCa                                                         <  1:650938/148‑1 (MQ=255)
                                                                                         gCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCa                                                         <  1:325818/148‑1 (MQ=255)
                                                                                          ccTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCag                                                        >  2:761386/1‑148 (MQ=255)
                                                                                                 gCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCa                                                 <  1:2613635/148‑1 (MQ=255)
                                                                                                   aaCGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAgg                                               <  1:1866257/148‑1 (MQ=255)
                                                                                                    aCGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGt                                              >  1:3515386/1‑148 (MQ=255)
                                                                                                     cGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGtt                                             <  2:2750852/148‑1 (MQ=255)
                                                                                                            tttGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAg                                      <  2:1784611/148‑1 (MQ=255)
                                                                                                             ttGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAgg                                     <  2:3439874/148‑1 (MQ=255)
                                                                                                             ttGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAgg                                     <  1:3065641/148‑1 (MQ=255)
                                                                                                             ttGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAgg                                     >  1:1408329/1‑148 (MQ=255)
                                                                                                             ttGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGATTCTGCAGCCGCCTCCAGAGCGCCCGGTTCGGAAAgg                                     >  1:555340/1‑148 (MQ=255)
                                                                                                             ttGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAgg                                     >  1:767885/1‑148 (MQ=255)
                                                                                                              tGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAggg                                    <  2:358209/148‑1 (MQ=255)
                                                                                                               gCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGa                                   <  2:1146081/148‑1 (MQ=255)
                                                                                                                cTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAg                                  <  1:2303550/148‑1 (MQ=255)
                                                                                                                 tCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGc                                 <  1:3510337/148‑1 (MQ=255)
                                                                                                                   aaTAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCgg                               <  1:2361147/148‑1 (MQ=255)
                                                                                                                    aTAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCACCAGAGCGCCAGGTTCGGAAAGGGAGCGGa                              >  2:1369974/1‑148 (MQ=255)
                                                                                                                     tAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGat                             <  2:1482149/148‑1 (MQ=255)
                                                                                                                     tAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGat                             <  1:3461512/148‑1 (MQ=255)
                                                                                                                     tAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGat                             <  1:1704109/148‑1 (MQ=255)
                                                                                                                       aaGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGatat                           <  1:1816375/148‑1 (MQ=255)
                                                                                                                        aGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATc                          >  2:339372/1‑148 (MQ=255)
                                                                                                                         gTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCa                         <  2:1016380/148‑1 (MQ=255)
                                                                                                                          tAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCAt                        >  1:3341912/1‑148 (MQ=255)
                                                                                                                           aGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCAtt                       >  2:1735501/1‑148 (MQ=255)
                                                                                                                            gCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGATCGGAAAGGGAGCGGATATCATta                      >  2:3295990/1‑148 (MQ=255)
                                                                                                                                ttCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATtaataa                  >  1:2226933/1‑148 (MQ=255)
                                                                                                                                ttCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATtaataa                  <  1:1751103/148‑1 (MQ=255)
                                                                                                                                ttCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATtaataa                  <  2:1380785/148‑1 (MQ=255)
                                                                                                                                ttCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATtaataa                  >  1:786882/1‑148 (MQ=255)
                                                                                                                                ttCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATtaataa                  >  1:2569172/1‑148 (MQ=255)
                                                                                                                                ttCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATtaataa                  >  2:1907552/1‑148 (MQ=255)
                                                                                                                                 tCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTaataat                 <  1:149808/148‑1 (MQ=255)
                                                                                                                                   acgtCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAAtgt               <  2:2038304/145‑1 (MQ=255)
                                                                                                                                     ttCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGa             <  2:3254632/148‑1 (MQ=255)
                                                                                                                                       cTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAgc           >  1:2800528/1‑148 (MQ=255)
                                                                                                                                              gACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACt    >  1:3285202/1‑148 (MQ=255)
                                                                                                                                              gACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACt    >  1:2504631/1‑148 (MQ=255)
                                                                                                                                              gACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACt    >  2:1438846/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCAGGCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTT  >  CP009273/3317658‑3317949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A