Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,223,839 G→A 39.3% D52N (GAT→AAT)  pgi → glucosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,223,8390GA39.3% 181.0 / 230.3 194D52N (GAT→AAT) pgiglucosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (63/54);  new base A (46/30);  total (109/85)
Fisher's exact test for biased strand distribution p-value = 3.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 196 total aligned reads displayed.

ACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGC  >  CP009273/4223693‑4223986
                                                                                                                                                  |                                                                                                                                                   
aCATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGa                                                                                                                                                    >  1:858541/1‑148 (MQ=255)
aCATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGa                                                                                                                                                    <  2:3502041/148‑1 (MQ=255)
aCATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGAGGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTATAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGa                                                                                                                                                    >  2:1067828/1‑148 (MQ=255)
   tCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTTAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTa                                                                                                                                                 <  1:1897436/148‑1 (MQ=255)
   tCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTa                                                                                                                                                 >  2:1846237/1‑148 (MQ=255)
       tCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTcc                                                                                                                                             >  2:576111/1‑148 (MQ=255)
         cAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCaa                                                                                                                                           <  1:590227/148‑1 (MQ=255)
         cAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCaa                                                                                                                                           >  1:2338223/1‑148 (MQ=255)
            cGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCaaaaa                                                                                                                                        >  1:571951/1‑148 (MQ=255)
             gCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAAc                                                                                                                                       <  2:2606829/148‑1 (MQ=255)
                  ccGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCAt                                                                                                                                  >  2:500942/1‑148 (MQ=255)
                  ccGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCAt                                                                                                                                  <  2:621280/148‑1 (MQ=255)
                   cGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATc                                                                                                                                 >  2:1746414/1‑148 (MQ=255)
                     cTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCAc                                                                                                                               <  1:1420337/148‑1 (MQ=255)
                     cTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCAc                                                                                                                               <  2:1597108/148‑1 (MQ=255)
                        ccTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGa                                                                                                                            <  2:2815880/148‑1 (MQ=255)
                        ccTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGa                                                                                                                            >  2:1671980/1‑148 (MQ=255)
                        ccTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGa                                                                                                                            >  2:661667/1‑148 (MQ=255)
                            gctggcaCTACAGAAACACTTCGATGAAATGAAAGACGGTACGATCGCCGATCTTTTTGCTAAAGACGGCGATAGTTTTTCTAAGTTATCAGCAACCTTCGACGATCTGATTCTGGTGGATTTATCCAAAAACGGCATCACTGAagag                                                                                                                        <  2:2271734/145‑1 (MQ=255)
                                   cTACAGAAACACTTCGATGAAATGAAAGACTTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGGTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTgg                                                                                                                 <  1:81809/148‑1 (MQ=255)
                                    tACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGc                                                                                                                <  1:2512090/148‑1 (MQ=255)
                                      cAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGa                                                                                                              >  1:1761905/1‑148 (MQ=255)
                                      cAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGa                                                                                                              >  2:2135611/1‑148 (MQ=255)
                                        gAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGaaa                                                                                                            <  1:1101622/148‑1 (MQ=255)
                                        gAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGaaa                                                                                                            <  1:465964/148‑1 (MQ=255)
                                        gAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGaaa                                                                                                            <  1:1101229/148‑1 (MQ=255)
                                        gAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAAACGCATCACTGAAGAGACGCTGGCGaaa                                                                                                            >  1:332431/1‑148 (MQ=255)
                                         aaaCACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAAt                                                                                                           >  2:2146731/1‑148 (MQ=255)
                                          aaCACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAAtt                                                                                                          <  1:1630789/148‑1 (MQ=255)
                                             acTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTAGACGATCAGATGCTGGTGAATTACTCCAAAAAACGCATCACTGAAGAGACGCTGGCGAAATTACa                                                                                                       >  1:3174186/1‑148 (MQ=255)
                                              cTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTTCTAAAGACGGCGATCGTTTTTATAAGTTGTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAg                                                                                                      >  1:31733/1‑148 (MQ=255)
                                              cTTCGATGAAATGAAAGACGTTACGATCGCCCAACTTTTTGCTAAAGACGGCGATCGTTTTTCTAATTTATCCGCAACCAACGACGATCAGATGCTGGTGGATTACTCCCAAAAACGCAAAACAGAACAGACGCTGGCGAAATTACac                                                                                                      >  1:33175/1‑147 (MQ=255)
                                               ttCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTTCTAAAGACGGCGATCGTTTTTATAAGTTCTACGCAACCTTCGAAGATCAGATGCTGGTGGATTACTACAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAgg                                                                                                     >  1:3218784/1‑148 (MQ=255)
                                                tCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGa                                                                                                    <  1:2306539/148‑1 (MQ=255)
                                                tCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCATCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGa                                                                                                    >  1:1391103/1‑148 (MQ=255)
                                                  gATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATc                                                                                                  <  2:173962/148‑1 (MQ=255)
                                                  gATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATc                                                                                                  <  2:1161576/148‑1 (MQ=255)
                                                   atgaaatgaaAGACGTTACGATCGCCGATCTTTTTTCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCAACACTGACGAGACGCTGGCGAAATTACAGGAGCt                                                                                                 >  1:2063425/1‑148 (MQ=255)
                                                   atgaaatgaaAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCt                                                                                                 <  2:2977740/148‑1 (MQ=255)
                                                     gaaatgaaaGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTgg                                                                                               >  2:3214666/1‑148 (MQ=255)
                                                     gaaatgaaaGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTgg                                                                                               >  2:1446561/1‑148 (MQ=255)
                                                     gaaatgaaaGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAACTTACAGGATCTgg                                                                                               >  2:2062230/1‑148 (MQ=255)
                                                     gaaatgaaaGACGTTACGATCGCCGAACTTTTTGCTAAATACGGCGAACGTTTTTCTAACTTCTCCGCAAAAATCCACTAACAGATGCAGGTGGATTAATCCAAAAAACGCATCACATAAGAGACCCAGGCGAAAAAAAAGGATATgg                                                                                               >  1:1331816/1‑148 (MQ=255)
                                                       aatgaaaGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCg                                                                                             <  2:2671612/148‑1 (MQ=255)
                                                             aGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAagag                                                                                       >  2:2769476/1‑148 (MQ=255)
                                                             aGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACAGAAGAGACGCTGGCGAAATTACAGGATCTGGCGCAagag                                                                                       >  2:1677094/1‑148 (MQ=255)
                                                              gACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGt                                                                                      <  1:2651103/148‑1 (MQ=255)
                                                                cGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGc                                                                                    <  2:204388/148‑1 (MQ=255)
                                                                cGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGc                                                                                    <  2:1869144/148‑1 (MQ=255)
                                                                   tACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAAACGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGAt                                                                                 >  1:197089/1‑148 (MQ=255)
                                                                    aCGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGCTc                                                                                >  2:3045328/1‑148 (MQ=255)
                                                                     cGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCt                                                                               >  1:1790620/1‑148 (MQ=255)
                                                                          gCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTggcgg                                                                          >  1:3010207/1‑148 (MQ=255)
                                                                           ccGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTggcggg                                                                         >  2:1841343/1‑148 (MQ=255)
                                                                              aTCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGgcgc                                                                      >  1:3133899/1‑148 (MQ=255)
                                                                               tCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGcgcg                                                                     >  1:79200/1‑148 (MQ=255)
                                                                                cTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGa                                                                    >  1:3367598/1‑148 (MQ=255)
                                                                                 tttttGCTAAAGACGGCGATCGATTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGAt                                                                   <  1:2002483/148‑1 (MQ=255)
                                                                                  ttttGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGagt                                                                  >  2:2130590/1‑146 (MQ=255)
                                                                                   tttGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTa                                                                 <  2:1427162/148‑1 (MQ=255)
                                                                                         aaaGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGa                                                           <  1:255826/148‑1 (MQ=255)
                                                                                           aGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATg                                                         >  2:2239085/1‑148 (MQ=255)
                                                                                            gACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGt                                                        <  1:2522105/148‑1 (MQ=255)
                                                                                            gACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGt                                                        >  2:1283355/1‑148 (MQ=255)
                                                                                             aCGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCAAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGtt                                                       >  2:3009858/1‑148 (MQ=255)
                                                                                              cGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTtc                                                      <  2:1534480/148‑1 (MQ=255)
                                                                                                 cGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTctct                                                   >  2:482789/1‑148 (MQ=255)
                                                                                                     cGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCg                                               >  2:2557190/1‑148 (MQ=255)
                                                                                                        ttttCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCgaga                                            <  1:3274822/148‑1 (MQ=255)
                                                                                                        ttttCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCgaga                                            <  2:2065417/148‑1 (MQ=255)
                                                                                                         tttCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGagaa                                           <  2:1790620/148‑1 (MQ=255)
                                                                                                          ttCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGagaag                                          <  1:1269964/148‑1 (MQ=255)
                                                                                                          ttCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGagaag                                          <  1:1928431/148‑1 (MQ=255)
                                                                                                           tCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGagaaga                                         <  2:1395546/148‑1 (MQ=255)
                                                                                                           tCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGagaaga                                         <  1:1426301/148‑1 (MQ=255)
                                                                                                            cTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGAt                                        <  2:250107/148‑1 (MQ=255)
                                                                                                              aaGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCa                                      >  2:2382451/1‑148 (MQ=255)
                                                                                                               aGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATcta                                     >  1:2239474/1‑146 (MQ=255)
                                                                                                               aGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCaa                                     <  1:2138340/148‑1 (MQ=255)
                                                                                                                gTTCTCCGCAACCTTCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAAc                                    >  2:3361506/1‑148 (MQ=255)
                                                                                                                   ctcCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGc                                 <  1:2029180/148‑1 (MQ=255)
                                                                                                                    tcCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCa                                <  2:3348692/148‑1 (MQ=255)
                                                                                                                       gCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTg                             <  1:2814216/148‑1 (MQ=255)
                                                                                                                        cAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGa                            >  1:2885423/1‑148 (MQ=255)
                                                                                                                         aaCCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGaa                           >  1:273830/1‑148 (MQ=255)
                                                                                                                           ccTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGaaaa                         <  1:2078503/148‑1 (MQ=255)
                                                                                                                             ttCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGAGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAAcc                       <  2:725302/148‑1 (MQ=255)
                                                                                                                             ttCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAAcc                       >  2:975849/1‑148 (MQ=255)
                                                                                                                             ttCGACGATCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAAcc                       <  2:2338223/148‑1 (MQ=255)
                                                                                                                                gacgaTCAGATGCTGGTGGATTACTCCAACAACCGCATCACTGCAGAGACGCTGGCGAAATGACAGGATCTGGCGACAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACCGAAAACcgcg                    <  2:1983742/148‑1 (MQ=255)
                                                                                                                                gacgaTAAGATGCTGGTGTATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGATATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCTATTAAGTCGATGTTCTCTGGCGAGAAGATCTACCGCACTGAAAACcgcg                    <  2:1724567/148‑1 (MQ=255)
                                                                                                                                   gaTCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCg                 <  1:354902/148‑1 (MQ=255)
                                                                                                                                   gaTCAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCg                 <  1:354896/148‑1 (MQ=255)
                                                                                                                                      cAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGtgc              <  2:1004419/148‑1 (MQ=255)
                                                                                                                                      cAGATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGtgc              >  1:2944422/1‑148 (MQ=255)
                                                                                                                                        gATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGtgctg            >  2:1950046/1‑148 (MQ=255)
                                                                                                                                        gATGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAAGTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGACGATCAACCGCACTGAAAACCGCGCCGtgctg            >  2:3177683/1‑148 (MQ=255)
                                                                                                                                         aTGCTGGTGAATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGtgctgc           >  1:2696829/1‑148 (MQ=255)
                                                                                                                                                 ggATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAgcg   <  1:1746414/148‑1 (MQ=255)
                                                                                                                                                  gATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAgcgc  >  1:3448038/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGC  >  CP009273/4223693‑4223986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A