Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 263,564 G→T 25.5% I51I (ATC→ATA)  yafZ ← CP4‑6 prophage; conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273263,5640GT25.5% 525.0 / 130.6 226I51I (ATC→ATA) yafZCP4‑6 prophage; conserved protein
Reads supporting (aligned to +/- strand):  ref base G (89/79);  new base T (32/26);  total (121/105)
Fisher's exact test for biased strand distribution p-value = 8.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

TTCCGGCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGTTTGCTGCGCCAAAGCGCGAAGCCAGA  >  CP009273/263417‑263708
                                                                                                                                                   |                                                                                                                                                
ttCCGGCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGgag                                                                                                                                                  <  1:63785/148‑1 (MQ=255)
ttCCGGCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGgag                                                                                                                                                  <  2:1770653/148‑1 (MQ=255)
ttCCGGCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATAGGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGgag                                                                                                                                                  >  1:626200/1‑148 (MQ=255)
    ggCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGAtgg                                                                                                                                              <  2:2693402/148‑1 (MQ=255)
     gCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATAtggt                                                                                                                                             >  2:2016966/1‑148 (MQ=255)
       aCCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGAtggtgg                                                                                                                                           <  1:1168296/148‑1 (MQ=255)
        ccTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTggg                                                                                                                                          <  1:144861/148‑1 (MQ=255)
             ttACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGtatat                                                                                                                                     <  1:904983/148‑1 (MQ=255)
             ttACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGtatat                                                                                                                                     <  2:2948725/148‑1 (MQ=255)
               aCCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAg                                                                                                                                   >  2:672116/1‑148 (MQ=255)
                ccGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAgg                                                                                                                                  <  2:276485/148‑1 (MQ=255)
                 cGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGt                                                                                                                                 <  2:1510312/148‑1 (MQ=255)
                  ggTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGta                                                                                                                                >  2:1092541/1‑148 (MQ=255)
                   gTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGtat                                                                                                                               >  2:224379/1‑148 (MQ=255)
                     gATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATaa                                                                                                                             <  1:955902/148‑1 (MQ=255)
                          gCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTc                                                                                                                        <  2:3494675/148‑1 (MQ=255)
                          gCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTc                                                                                                                        <  2:317149/148‑1 (MQ=255)
                           cccTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATCTAGGTATAACGCTCa                                                                                                                       >  1:2634723/1‑148 (MQ=255)
                           cccTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCa                                                                                                                       <  1:3064794/148‑1 (MQ=255)
                            ccTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCAc                                                                                                                      >  2:3153176/1‑148 (MQ=255)
                             cTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACt                                                                                                                     >  1:1564683/1‑148 (MQ=255)
                             cTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACt                                                                                                                     >  2:2555532/1‑148 (MQ=255)
                               tCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTAc                                                                                                                   >  1:3015925/1‑148 (MQ=255)
                                 ccgccgCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACgg                                                                                                                 >  1:3290661/1‑148 (MQ=255)
                                   gccgcAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGa                                                                                                               <  2:573111/148‑1 (MQ=255)
                                   gccgcAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGa                                                                                                               <  2:541923/148‑1 (MQ=255)
                                   gccgcAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGa                                                                                                               <  2:2414120/148‑1 (MQ=255)
                                   gccgcAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGa                                                                                                               <  1:2808442/148‑1 (MQ=255)
                                      gcAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTc                                                                                                            >  1:2705453/1‑148 (MQ=255)
                                        aGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCgt                                                                                                          >  1:3271338/1‑148 (MQ=255)
                                        aGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCgt                                                                                                          >  1:3443931/1‑148 (MQ=255)
                                         gACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCgtg                                                                                                         <  1:1460126/148‑1 (MQ=255)
                                          aCGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCgtgt                                                                                                        <  1:802897/148‑1 (MQ=255)
                                          aCGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCgtgt                                                                                                        >  2:2935584/1‑148 (MQ=255)
                                             cagcaTATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTg                                                                                                     >  2:2338103/1‑148 (MQ=255)
                                               gcaTATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTc                                                                                                   >  2:1001669/1‑148 (MQ=255)
                                                  tatGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTc                                                                                                <  1:1025713/148‑1 (MQ=255)
                                                  tatGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTc                                                                                                <  1:2945639/148‑1 (MQ=255)
                                                    tGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCAc                                                                                              >  1:1427858/1‑148 (MQ=255)
                                                    tGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCAc                                                                                              >  2:535383/1‑148 (MQ=255)
                                                    tGCTTTGTATGTTCACGACGACCCGGGTCACGCACGAGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCAc                                                                                              >  2:1533198/1‑148 (MQ=255)
                                                           tATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATac                                                                                       <  1:340585/148‑1 (MQ=255)
                                                            aTGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGCCTCGTGTTTGTCCTCACTGAATaca                                                                                      >  1:843616/1‑148 (MQ=255)
                                                            aTGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATaca                                                                                      <  2:2896878/148‑1 (MQ=255)
                                                            aTGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATaca                                                                                      <  1:3056650/148‑1 (MQ=255)
                                                             tGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACGACGGGACTCGTGTTTGTCCTCACTGAATacac                                                                                     <  1:1824653/148‑1 (MQ=255)
                                                              gTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACt                                                                                    >  1:3258529/1‑148 (MQ=255)
                                                              gTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACt                                                                                    >  1:2437086/1‑148 (MQ=255)
                                                               ttCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTg                                                                                   >  1:2844374/1‑148 (MQ=255)
                                                                 cACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTggg                                                                                 <  1:2520317/148‑1 (MQ=255)
                                                                   cgacgaCCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCa                                                                               <  1:2884920/148‑1 (MQ=255)
                                                                   cgacgaCCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCa                                                                               <  2:1821966/148‑1 (MQ=255)
                                                                        acCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTAcg                                                                          >  2:2895804/1‑148 (MQ=255)
                                                                        acCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTAcg                                                                          >  2:2783328/1‑148 (MQ=255)
                                                                         cccGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTAcgc                                                                         >  2:3003790/1‑148 (MQ=255)
                                                                         cccGGGTCACGCACGCGAGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTAcgc                                                                         <  1:25892/148‑1 (MQ=255)
                                                                          ccGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTAcgcg                                                                        >  2:3290692/1‑148 (MQ=255)
                                                                            gggTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGaa                                                                      <  2:514412/148‑1 (MQ=255)
                                                                            gggTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGaa                                                                      <  1:1775522/148‑1 (MQ=255)
                                                                              gTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAAc                                                                    >  1:2292930/1‑148 (MQ=255)
                                                                               tCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACa                                                                   >  1:1787639/1‑148 (MQ=255)
                                                                                cacgcacgCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAg                                                                  >  1:2794689/1‑148 (MQ=255)
                                                                                  cgcacgcGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGct                                                                <  1:2466065/148‑1 (MQ=255)
                                                                                   gcacgcGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGctc                                                               <  1:3202985/148‑1 (MQ=255)
                                                                                     acgcGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCtt                                                             <  1:3163187/148‑1 (MQ=255)
                                                                                           gTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACggg                                                       <  2:3139514/148‑1 (MQ=255)
                                                                                             cTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGtt                                                     <  1:1946955/148‑1 (MQ=255)
                                                                                             cTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGtt                                                     >  1:1149906/1‑148 (MQ=255)
                                                                                               gACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTaa                                                   >  1:3532526/1‑148 (MQ=255)
                                                                                                  aGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAacgg                                                >  1:349673/1‑148 (MQ=255)
                                                                                                  aGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAacgg                                                <  1:1424387/148‑1 (MQ=255)
                                                                                                    gCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAacggac                                              >  2:1275565/1‑148 (MQ=255)
                                                                                                      aaaGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACAATGGGCACTACGCGAAACAGCTCTTCACGGGTTAacggacgg                                            <  1:1262607/148‑1 (MQ=255)
                                                                                                         gAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTcg                                         >  1:1730753/1‑148 (MQ=255)
                                                                                                         gAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTcg                                         >  1:2693890/1‑148 (MQ=255)
                                                                                                         gAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTcg                                         <  2:2096626/148‑1 (MQ=255)
                                                                                                         gAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTcg                                         >  1:812683/1‑148 (MQ=255)
                                                                                                          aaTGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTCCGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTcgc                                        >  2:584992/1‑148 (MQ=255)
                                                                                                            tGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGa                                      <  1:51119/148‑1 (MQ=255)
                                                                                                               cTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACgg                                   <  1:1596555/148‑1 (MQ=255)
                                                                                                                 ggAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCTACGGAt                                 <  2:1041738/148‑1 (MQ=255)
                                                                                                                 ggAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCTACGGAt                                 <  2:1418997/148‑1 (MQ=255)
                                                                                                                  gAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATa                                >  2:2418242/1‑148 (MQ=255)
                                                                                                                   aaGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATaa                               <  2:626200/148‑1 (MQ=255)
                                                                                                                    aGCCTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAg                              <  2:395608/148‑1 (MQ=255)
                                                                                                                     gCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAgg                             >  1:2198219/1‑148 (MQ=255)
                                                                                                                      ccTTCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGt                            <  2:3461324/148‑1 (MQ=255)
                                                                                                                      ccTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGt                            >  1:1488164/1‑148 (MQ=255)
                                                                                                                       cTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGtt                           <  2:3341480/148‑1 (MQ=255)
                                                                                                                        ttCTCGCTGTAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGttt                          >  1:2582698/1‑148 (MQ=255)
                                                                                                                            cGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGTTtgctg                      >  2:3087508/1‑148 (MQ=255)
                                                                                                                                gTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGTTTGCTGCGcc                  >  2:806928/1‑148 (MQ=255)
                                                                                                                                 tAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGTTTGCTGCGCCa                 >  1:3089541/1‑148 (MQ=255)
                                                                                                                                 tAGGCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGTTTGCTGCGCCa                 >  1:2232642/1‑148 (MQ=255)
                                                                                                                                 tAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGTTTGCTGCGCCa                 >  1:3147781/1‑148 (MQ=255)
                                                                                                                                 tAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGTTTGCTGCGCCa                 >  1:2669842/1‑148 (MQ=255)
                                                                                                                                    gCTGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGTTTGCTGCGCCAAAg              >  1:1099291/1‑148 (MQ=255)
                                                                                                                                    gCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGTTTGCTGCGCCAAAg              <  1:361006/148‑1 (MQ=255)
                                                                                                                                      tGTCGAGCAGGGATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGTTTGCTGCGCCAAAgcg            <  2:789937/148‑1 (MQ=255)
                                                                                                                                                ggATATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGTTTGCTGCGCCAAAGCGCGAAGCCAGa  >  1:1100731/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TTCCGGCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTGACAGGCAAAGAATGGCTGGAAGCCTTCTCGCTGTAGGCTGTCGAGCAGGGAGATGGTGGGTATATAGGTATAACGCTCACTACGGGACTCGTGTTTGTCCTCACTGAATACACTGGGCACTACGCGAAACAGCTCTTCACGGGTTAACGGACGGTCGCGACGGATAAGGTTTGCTGCGCCAAAGCGCGAAGCCAGA  >  CP009273/263417‑263708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A