Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,116,548 T→A 100% intergenic (‑137/+150) bssS ← / ← dinI biofilm regulator/DNA damage‑inducible protein I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,116,5480TA96.8% 740.1 / 9.1 230intergenic (‑137/+150)bssS/dinIbiofilm regulator/DNA damage‑inducible protein I
Reads supporting (aligned to +/- strand):  ref base T (1/7);  new base A (111/111);  total (112/118)
Fisher's exact test for biased strand distribution p-value = 6.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 233 total aligned reads displayed.

TTTTTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACT  >  CP009273/1116403‑1116692
                                                                                                                                                 |                                                                                                                                                
tttgtCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTaaa                                                                                                                                                <  1:1268511/148‑1 (MQ=255)
   ttCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTTAAGAt                                                                                                                                             <  1:1224932/148‑1 (MQ=255)
    tCCATAATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTAAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGAtg                                                                                                                                            >  2:2211164/1‑147 (MQ=255)
      catcatGGCCCCCTAATTCGTGTTACTAGACAACGTTACTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTta                                                                                                                                          <  1:2119230/148‑1 (MQ=255)
       atcatGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTtat                                                                                                                                         <  2:3302942/148‑1 (MQ=255)
       atcatGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTtat                                                                                                                                         <  1:2974440/148‑1 (MQ=255)
           tGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGa                                                                                                                                     >  2:3519806/1‑148 (MQ=255)
           tGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGa                                                                                                                                     <  1:3084028/148‑1 (MQ=255)
           tGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTAACCGAATTTTTCATGTGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGa                                                                                                                                     >  2:3519570/1‑148 (MQ=255)
            ggCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGaa                                                                                                                                    <  2:2060473/148‑1 (MQ=255)
             gCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAAc                                                                                                                                   >  2:1317779/1‑148 (MQ=255)
              cccccTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACa                                                                                                                                  <  2:3113337/148‑1 (MQ=255)
                cccTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAAt                                                                                                                                >  1:3219376/1‑148 (MQ=255)
                cccTAATTCGTCTGACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAAt                                                                                                                                <  1:1847910/148‑1 (MQ=255)
                 ccTAATTCGTGTTACTAGACAACGTTATTCGAGTTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGATTTATAGATTTATATGAACAATa                                                                                                                               >  1:3036023/1‑148 (MQ=255)
                  atAATACGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGTGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATaa                                                                                                                              <  2:3385700/147‑1 (MQ=255)
                   tAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATaaa                                                                                                                             <  2:2418093/148‑1 (MQ=255)
                    aaTTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATaaaa                                                                                                                            <  2:2407400/148‑1 (MQ=255)
                     aTTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTTAAGATTTATATGAACAATAAAAc                                                                                                                           <  2:952067/148‑1 (MQ=255)
                     aTTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAAc                                                                                                                           >  2:1618066/1‑148 (MQ=255)
                     aTTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAAc                                                                                                                           >  2:1617417/1‑148 (MQ=255)
                       tCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAg                                                                                                                         <  1:603904/148‑1 (MQ=255)
                           gtTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTTAAGATTTATATGAACAATAAAACAGCATg                                                                                                                     >  2:418963/1‑148 (MQ=255)
                           gtTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATg                                                                                                                     >  1:459871/1‑148 (MQ=255)
                           gtTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATg                                                                                                                     >  1:3261614/1‑148 (MQ=255)
                             tACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGtc                                                                                                                   >  2:3010668/1‑148 (MQ=255)
                              aCTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGtca                                                                                                                  <  1:2716770/148‑1 (MQ=255)
                              aCTAGAAAACGTTATTAGAGGTTAAATGCGTTTTTATCTGAAGCATCTTCAGTATACCAATTAGTAAGGGTATTCACCTTATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACTATAAAACAGCATGtca                                                                                                                  <  2:2376731/148‑1 (MQ=255)
                               cTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGtcat                                                                                                                 <  1:1568221/148‑1 (MQ=255)
                                    cAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCata                                                                                                            <  2:1083496/148‑1 (MQ=255)
                                    cAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCata                                                                                                            <  2:3166707/148‑1 (MQ=255)
                                     aaCGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCatat                                                                                                           <  1:2036271/148‑1 (MQ=255)
                                      aCGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATAtt                                                                                                          >  2:2755764/1‑148 (MQ=255)
                                       cGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAAc                                                                                                                           >  1:3433494/1‑130 (MQ=255)
                                       cGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATAttt                                                                                                         <  1:1112699/148‑1 (MQ=255)
                                       cGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTAAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATAttt                                                                                                         >  2:3368993/1‑148 (MQ=255)
                                              cGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGc                                                                                                  <  1:1561254/148‑1 (MQ=255)
                                                  gTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCatat                                                                                              <  1:3300304/148‑1 (MQ=255)
                                                  gTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCatat                                                                                              >  2:2268143/1‑148 (MQ=255)
                                                   ttCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATAtt                                                                                             >  2:552034/1‑148 (MQ=255)
                                                    tCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATtg                                                                                            >  1:828659/1‑148 (MQ=255)
                                                    tCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATtg                                                                                            <  2:2499811/148‑1 (MQ=255)
                                                      aaTGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATtgtg                                                                                          <  1:1124266/148‑1 (MQ=255)
                                                        tGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCa                                                                                        >  1:1040393/1‑148 (MQ=255)
                                                        tGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCa                                                                                        >  2:1062236/1‑148 (MQ=255)
                                                         gCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCaa                                                                                       >  1:1711168/1‑148 (MQ=255)
                                                         gCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCaa                                                                                       >  1:1912850/1‑148 (MQ=255)
                                                         gCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCaa                                                                                       >  1:1368423/1‑148 (MQ=255)
                                                         gCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCaa                                                                                       >  2:1901777/1‑148 (MQ=255)
                                                         gCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCaa                                                                                       >  1:3556522/1‑148 (MQ=255)
                                                          cGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAat                                                                                      <  2:1214526/148‑1 (MQ=255)
                                                             ttttATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAattat                                                                                   <  2:1098227/148‑1 (MQ=255)
                                                              tttATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAattatt                                                                                  >  1:1583448/1‑148 (MQ=255)
                                                                 aTCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTg                                                                               <  1:571346/148‑1 (MQ=255)
                                                                   cTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAg                                                                             <  1:3397337/148‑1 (MQ=255)
                                                                    tGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAgg                                                                            >  1:2683226/1‑148 (MQ=255)
                                                                     gAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGa                                                                           >  1:1739106/1‑148 (MQ=255)
                                                                       aGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAg                                                                         <  1:1827017/148‑1 (MQ=255)
                                                                        gCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAgt                                                                        >  1:724747/1‑148 (MQ=255)
                                                                              tCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGaa                                                                  <  1:666530/148‑1 (MQ=255)
                                                                                 gTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAAtt                                                               >  2:2744472/1‑148 (MQ=255)
                                                                                    taCCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTg                                                            <  2:3261614/148‑1 (MQ=255)
                                                                                     aCCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGt                                                           >  1:383223/1‑148 (MQ=255)
                                                                                     aCCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGt                                                           >  2:1666889/1‑148 (MQ=255)
                                                                                         aTAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTc                                                       >  1:2236665/1‑148 (MQ=255)
                                                                                          taagtaagGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCa                                                      <  1:454624/148‑1 (MQ=255)
                                                                                            agtaagGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCaaa                                                    >  2:554468/1‑148 (MQ=255)
                                                                                             gtaagGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCaaaa                                                   <  2:1574957/148‑1 (MQ=255)
                                                                                                 gggTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTATAAATTTTGTACTCAAAAttag                                               >  2:2617956/1‑146 (MQ=255)
                                                                                                    tATTCACCGAATTTTTACAGGGTGAAACAGATAAATTTATGCTTTAAAGATTTATATGAACAATAACACATCCTGTCATTCATATTTTTTTAGCATATGGTGCAATTATTTTGAGGACGTGTAGAAATTTTGTACTCAAAATTCgtaa                                            <  2:3036023/148‑1 (MQ=255)
                                                                                                      ttCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCgtaagt                                          >  2:3415722/1‑148 (MQ=255)
                                                                                                      ttCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCgtaagt                                          <  1:390042/148‑1 (MQ=255)
                                                                                                       tCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCgtaagta                                         <  2:3327656/148‑1 (MQ=255)
                                                                                                       tCACAGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCgtaagta                                         >  2:1539532/1‑148 (MQ=255)
                                                                                                        cACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCgtaagtaa                                        >  1:2057449/1‑148 (MQ=255)
                                                                                                            gAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGtaaaata                                    <  2:1778646/148‑1 (MQ=255)
                                                                                                             aaTTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGtaaaataa                                   <  1:418963/148‑1 (MQ=255)
                                                                                                             aaTTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGtaaaataa                                   <  1:1640099/148‑1 (MQ=255)
                                                                                                              aTTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGtaaaataaa                                  >  1:2776951/1‑148 (MQ=255)
                                                                                                                ttttAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTACGTAACAGaaaaa                                >  2:3103419/1‑148 (MQ=255)
                                                                                                                  ttAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAg                              <  1:796516/148‑1 (MQ=255)
                                                                                                                     aaGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCg                           <  2:724747/148‑1 (MQ=255)
                                                                                                                      aGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCgg                          >  2:2943185/1‑148 (MQ=255)
                                                                                                                       gggggAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCggg                         >  1:196863/1‑148 (MQ=255)
                                                                                                                       gggggAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCggg                         >  2:2256176/1‑148 (MQ=255)
                                                                                                                        ggggAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCgggg                        >  2:1356342/1‑148 (MQ=255)
                                                                                                                           tatctataTAATTTTCTGCTTTAAATATTTATATTAACAAAAAAACAGAATGTAATTAATTTTTTTTTAGCATTTTGTGAAATTATTTTGAGGACGTGTAGAAATTTTTTACTAAAATTTCGTAAGTAAAATAAAAAAGCCGGGGCGa                     <  1:2189243/141‑1 (MQ=255)
                                                                                                                           gAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGa                     >  1:3345119/1‑148 (MQ=255)
                                                                                                                              aCAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGAccc                  >  1:2318261/1‑148 (MQ=255)
                                                                                                                               cAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCg                 <  2:833707/148‑1 (MQ=255)
                                                                                                                                  aTAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGAccagatc              >  2:2189243/1‑143 (MQ=255)
                                                                                                                                  aTAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCa              >  1:1160311/1‑148 (MQ=255)
                                                                                                                                   tAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCaa             >  2:621082/1‑148 (MQ=255)
                                                                                                                                          cTGCTTTAAAGATTTATATGAACAATAAAAAAGCATTTAATTAATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAAt      <  2:1367944/148‑1 (MQ=255)
                                                                                                                                           tGCTTTAAAGATTTATATGAACGATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATc     >  2:1418222/1‑148 (MQ=255)
                                                                                                                                           tGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGACGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATc     <  1:1982974/148‑1 (MQ=255)
                                                                                                                                           tGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATc     >  2:2157007/1‑148 (MQ=255)
                                                                                                                                            gCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCa    >  2:1253787/1‑148 (MQ=255)
                                                                                                                                             cTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAACATCAc   >  2:1879683/1‑148 (MQ=255)
                                                                                                                                             cTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCAc   >  1:207176/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTTTTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACT  >  CP009273/1116403‑1116692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTTTTTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATA  >  CP009273/1116400‑1116697
                                                                                                                                                    |                                                                                                                                                     
ATTTTTTTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAA                                                                                                                                                      <  SRR19973066.1127260/150‑1 (MQ=60)
ATTTTTTTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATATCTGCTTTAA                                                                                                                                                      >  SRR19973066.2288450/1‑150 (MQ=60)
  TTTTTTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAG                                                                                                                                                    <  SRR19973066.2139774/150‑1 (MQ=60)
  TTTTTTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAG                                                                                                                                                    <  SRR19973066.331801/150‑1 (MQ=60)
   TTTGTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGA                                                                                                                                                   <  SRR19973066.1277671/150‑1 (MQ=60)
     tccgatctATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATT                                                                                                                                                 <  SRR19973066.70425/142‑1 (MQ=60)
      TTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTTAAGATTT                                                                                                                                                <  SRR19973066.1231822/150‑1 (MQ=60)
      TTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTTAAGATTT                                                                                                                                                <  SRR19973066.1233604/150‑1 (MQ=60)
       TCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTA                                                                                                                                               <  SRR19973066.3501337/150‑1 (MQ=60)
         CATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTACTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATA                                                                                                                                             <  SRR19973066.2133877/150‑1 (MQ=60)
          ATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATAT                                                                                                                                            <  SRR19973066.2994098/150‑1 (MQ=60)
             ATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAA                                                                                                                                         >  SRR19973066.142579/1‑150 (MQ=60)
              TGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAAC                                                                                                                                        <  SRR19973066.1764053/150‑1 (MQ=60)
              TGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAAC                                                                                                                                        <  SRR19973066.3104633/150‑1 (MQ=60)
              TGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAAC                                                                                                                                        <  SRR19973066.3536492/150‑1 (MQ=60)
                 CCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAAT                                                                                                                                     >  SRR19973066.3240680/1‑150 (MQ=60)
                 CCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAAT                                                                                                                                     >  SRR19973066.3240643/1‑150 (MQ=60)
                 CCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAAT                                                                                                                                     >  SRR19973066.3073332/1‑150 (MQ=60)
                 CCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAAT                                                                                                                                     >  SRR19973066.427763/1‑150 (MQ=60)
                 CCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGATTTAAAGATTTATATGAACAAT                                                                                                                                     >  SRR19973066.3240243/1‑150 (MQ=60)
                  CCCCTAATTCGTGTTACTAGACAACGTTATTCGAGTTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGATTTATAGATTTATATGAACAATA                                                                                                                                    >  SRR19973066.3056314/1‑150 (MQ=60)
                   CCCTAATTCGTCTGACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAA                                                                                                                                   <  SRR19973066.1861136/150‑1 (MQ=60)
                      TAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAAC                                                                                                                                <  SRR19973066.1955568/150‑1 (MQ=60)
                       AATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACA                                                                                                                               <  SRR19973066.1645639/150‑1 (MQ=60)
                       AATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACA                                                                                                                               >  SRR19973066.1791359/1‑150 (MQ=60)
                        ATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAG                                                                                                                              <  SRR19973066.419189/150‑1 (MQ=60)
                          TCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCA                                                                                                                            <  SRR19973066.608067/150‑1 (MQ=60)
                          TCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCA                                                                                                                            <  SRR19973066.608091/150‑1 (MQ=60)
                            GTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATG                                                                                                                          >  SRR19973066.463153/1‑150 (MQ=60)
                            GTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATG                                                                                                                          >  SRR19973066.463393/1‑150 (MQ=60)
                            GTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATG                                                                                                                          >  SRR19973066.3283062/1‑150 (MQ=60)
                                 ACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATT                                                                                                                     <  SRR19973066.2735028/150‑1 (MQ=60)
                                 ACTAGACAACGTTATTCGAGGTTCAATGAGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATT                                                                                                                     <  SRR19973066.2869913/150‑1 (MQ=60)
                                  CTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTC                                                                                                                    <  SRR19973066.1579520/150‑1 (MQ=60)
                                  CTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTC                                                                                                                    <  SRR19973066.1578887/150‑1 (MQ=60)
                                   TAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCA                                                                                                                   >  SRR19973066.398151/1‑150 (MQ=60)
                                        AACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGagatcggaagagcaca                                                                                                              >  SRR19973066.3456467/1‑134 (MQ=60)
                                        AACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTT                                                                                                              <  SRR19973066.2050473/150‑1 (MQ=60)
                                          CGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTT                                                                                                            <  SRR19973066.1120739/150‑1 (MQ=60)
                                          CGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTT                                                                                                            <  SRR19973066.20740/150‑1 (MQ=60)
                                          CGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTT                                                                                                            <  SRR19973066.20737/150‑1 (MQ=60)
                                           GTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTT                                                                                                           <  SRR19973066.1327328/150‑1 (MQ=60)
                                             TATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTA                                                                                                         >  SRR19973066.93415/1‑150 (MQ=60)
                                                 CGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCAT                                                                                                     <  SRR19973066.1572492/150‑1 (MQ=60)
                                                  GATGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAATTAAGTTTATTCAGCGAATTTTTACAGGGGGAAACAGATAAATTTCTGCTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATA                                                                                                    <  SRR19973066.758708/150‑1 (MQ=60)
                                                   AGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATAT                                                                                                   <  SRR19973066.2338665/150‑1 (MQ=60)
                                                   AGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATAT                                                                                                   >  SRR19973066.501418/1‑150 (MQ=60)
                                                     GTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTG                                                                                                 <  SRR19973066.1125283/150‑1 (MQ=60)
                                                     GTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTG                                                                                                 >  SRR19973066.834706/1‑150 (MQ=60)
                                                     GTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTG                                                                                                 <  SRR19973066.3322124/150‑1 (MQ=60)
                                                       TCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTG                                                                                               <  SRR19973066.1174612/150‑1 (MQ=60)
                                                        CAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGC                                                                                              >  SRR19973066.1093389/1‑150 (MQ=60)
                                                         AATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCA                                                                                             >  SRR19973066.1048048/1‑150 (MQ=60)
                                                         AATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCA                                                                                             <  SRR19973066.1132358/150‑1 (MQ=60)
                                                          ATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAA                                                                                            >  SRR19973066.1378324/1‑150 (MQ=60)
                                                          ATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAA                                                                                            >  SRR19973066.3580112/1‑150 (MQ=60)
                                                          ATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAA                                                                                            >  SRR19973066.1723584/1‑150 (MQ=60)
                                                          ATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAA                                                                                            >  SRR19973066.1724231/1‑150 (MQ=60)
                                                          ATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAA                                                                                            >  SRR19973066.1926472/1‑150 (MQ=60)
                                                              GTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTAT                                                                                        >  SRR19973066.3364670/1‑150 (MQ=60)
                                                               TTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATT                                                                                       >  SRR19973066.1594893/1‑150 (MQ=60)
                                                                 TTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTT                                                                                     <  SRR19973066.2676388/150‑1 (MQ=60)
                                                                    ATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAG                                                                                  <  SRR19973066.575491/150‑1 (MQ=60)
                                                                    ATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAG                                                                                  <  SRR19973066.575381/150‑1 (MQ=60)
                                                                    ATCTGAACCAACTTCAGTATACCAATAACTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAG                                                                                  <  SRR19973066.1928044/150‑1 (MQ=60)
                                                                     TCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGG                                                                                 >  SRR19973066.2701132/1‑150 (MQ=60)
                                                                      CTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGA                                                                                <  SRR19973066.3420004/150‑1 (MQ=60)
                                                                      CTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGA                                                                                >  SRR19973066.1751655/1‑150 (MQ=60)
                                                                       TGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAA                                                                               <  SRR19973066.220361/150‑1 (MQ=60)
                                                                         AAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGT                                                                             >  SRR19973066.730121/1‑150 (MQ=60)
                                                                          AGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTG                                                                            <  SRR19973066.2137662/150‑1 (MQ=60)
                                                                          AGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTG                                                                            <  SRR19973066.1902989/150‑1 (MQ=60)
                                                                          AGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTG                                                                            <  SRR19973066.1840050/150‑1 (MQ=60)
                                                                          AGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTG                                                                            <  SRR19973066.1846094/150‑1 (MQ=60)
                                                                               CTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAA                                                                       >  SRR19973066.2951699/1‑150 (MQ=60)
                                                                                 TCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAAT                                                                     <  SRR19973066.671397/150‑1 (MQ=60)
                                                                                 TCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAAT                                                                     <  SRR19973066.671453/150‑1 (MQ=60)
                                                                                      ATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGT                                                                >  SRR19973066.2253480/1‑150 (MQ=60)
                                                                                      ATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGT                                                                >  SRR19973066.386387/1‑150 (MQ=60)
                                                                                      ATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGT                                                                >  SRR19973066.384499/1‑150 (MQ=60)
                                                                                      ATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGT                                                                >  SRR19973066.386248/1‑150 (MQ=60)
                                                                                         CCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACT                                                             <  SRR19973066.3435348/150‑1 (MQ=60)
                                                                                          CAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTC                                                            >  SRR19973066.2251205/1‑150 (MQ=60)
                                                                                          CAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTC                                                            >  SRR19973066.2252094/1‑150 (MQ=60)
                                                                                           AATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCA                                                           <  SRR19973066.3403904/150‑1 (MQ=60)
                                                                                             TAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAA                                                         <  SRR19973066.458119/150‑1 (MQ=60)
                                                                                                     GGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAA                                                 >  SRR19973066.2723816/1‑150 (MQ=60)
                                                                                                      GTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAG                                                <  SRR19973066.2774326/150‑1 (MQ=60)
                                                                                                      GTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAG                                                <  SRR19973066.2009613/150‑1 (MQ=60)
                                                                                                       TATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGT                                               >  SRR19973066.2090393/1‑150 (MQ=60)
                                                                                                         TTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAA                                             <  SRR19973066.1702037/150‑1 (MQ=60)
                                                                                                         TTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAA                                             <  SRR19973066.393120/150‑1 (MQ=60)
                                                                                                         TTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAA                                             <  SRR19973066.1606511/150‑1 (MQ=60)
                                                                                                         TTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAA                                             >  SRR19973066.2071723/1‑150 (MQ=60)
                                                                                                         TTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAA                                             <  SRR19973066.52242/150‑1 (MQ=60)
                                                                                                               GAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAA                                       >  SRR19973066.1004408/1‑150 (MQ=60)
                                                                                                               GAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAA                                       >  SRR19973066.2795645/1‑150 (MQ=60)
                                                                                                                AATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAA                                      <  SRR19973066.422228/150‑1 (MQ=60)
                                                                                                                AATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAA                                      <  SRR19973066.1652028/150‑1 (MQ=60)
                                                                                                                     TTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCC                                 <  SRR19973066.802546/150‑1 (MQ=60)
                                                                                                                     TTAAAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCC                                 <  SRR19973066.802364/150‑1 (MQ=60)
                                                                                                                        AAGGGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGG                              >  SRR19973066.198550/1‑150 (MQ=60)
                                                                                                                           GGGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCG                           >  SRR19973066.1450378/1‑150 (MQ=60)
                                                                                                                            GGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGA                          >  SRR19973066.3367389/1‑150 (MQ=60)
                                                                                                                            GGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGA                          >  SRR19973066.3366756/1‑150 (MQ=60)
                                                                                                                            GGGAAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGA                          >  SRR19973066.1588032/1‑150 (MQ=60)
                                                                                                                              tatctatataatTTTCTGCTTTAAATATTTATATTAACAAAAAAACAGAATGTAATTAATTTTTTTTTAGCATTTTGTGAAATTATTTTGAGGACGTGTAGAAATTTTTTACTAAAATTTCGTAAGTAAAATAAAAAAGCCGGGGCGACC                        <  SRR19973066.2204429/138‑1 (MQ=60)
                                                                                                                               AAACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCC                       >  SRR19973066.2334111/1‑150 (MQ=60)
                                                                                                                                AACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCG                      >  SRR19973066.1272781/1‑150 (MQ=60)
                                                                                                                                 ACAGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGG                     >  SRR19973066.3307416/1‑150 (MQ=60)
                                                                                                                                   AGATAAATTTCTGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCA                   >  SRR19973066.1168543/1‑150 (MQ=60)
                                                                                                                                              TGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGACGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCAC        <  SRR19973066.1996965/150‑1 (MQ=60)
                                                                                                                                              TGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCAC        >  SRR19973066.208901/1‑150 (MQ=60)
                                                                                                                                              TGCTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCAC        >  SRR19973066.208912/1‑150 (MQ=60)
                                                                                                                                              TGCTTTAAAGATTTATATGAACAAAAAAACAGCATGTCATTCATATTTTTTTAGAATTTTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCTTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCAC        <  SRR19973066.327044/150‑1 (MQ=60)
                                                                                                                                                CTTTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTG      <  SRR19973066.2997820/150‑1 (MQ=60)
                                                                                                                                                 TTTAAAGATTTATATGAACAATAAAACAGCATGTCATTAATTTTTTTTTAGCATTTTGTGCAATTATTTTGAGGAAGTGTACAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGC     <  SRR19973066.1133387/150‑1 (MQ=60)
                                                                                                                                                  TTAAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCA    >  SRR19973066.3356473/1‑150 (MQ=60)
                                                                                                                                                   TAAAGATTTATATCAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGTAAAAAAAATCACTGCAT   <  SRR19973066.1070026/150‑1 (MQ=60)
                                                                                                                                                   TAAAGATTAATATCAACAATAAAACAGCATGTCATTAATATTTTTTTAGCATATTGTGCATTTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCAT   <  SRR19973066.2848384/150‑1 (MQ=60)
                                                                                                                                                    AAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATA  >  SRR19973066.3569529/1‑150 (MQ=60)
                                                                                                                                                    AAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATA  >  SRR19973066.3569457/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ATTTTTTTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATA  >  CP009273/1116400‑1116697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑