Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,002,354 C→A 94.7% L199L (CTC→CTA)  yedE → UPF0394 family sulfur transport domain‑containing inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,002,3540CA94.7% 405.8 / 7.9 163L199L (CTC→CTA) yedEUPF0394 family sulfur transport domain‑containing inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (7/4);  new base A (59/90);  total (69/94)
Fisher's exact test for biased strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

TGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCG  >  CP009273/2002210‑2002497
                                                                                                                                                |                                                                                                                                               
tGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAggg                                                                                                                                              <  2:3041217/148‑1 (MQ=255)
   cATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATg                                                                                                                                           >  1:2591764/1‑148 (MQ=255)
   cATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATg                                                                                                                                           <  2:1052081/148‑1 (MQ=255)
   cATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCACGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATg                                                                                                                                           <  1:267528/148‑1 (MQ=255)
    aTCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGc                                                                                                                                          >  2:551608/1‑148 (MQ=255)
    aTCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCGAGGGATgg                                                                                                                                          >  1:2272004/1‑147 (MQ=255)
        ccACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGt                                                                                                                                      <  1:423738/148‑1 (MQ=255)
            tGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCttt                                                                                                                                  >  2:402331/1‑148 (MQ=255)
              ccATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCttttt                                                                                                                                <  1:788793/148‑1 (MQ=255)
              ccATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCttttt                                                                                                                                <  2:2106401/148‑1 (MQ=255)
              ccATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCttttt                                                                                                                                <  1:789018/148‑1 (MQ=255)
                aTTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCg                                                                                                                              >  2:3042326/1‑148 (MQ=255)
                 ttGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCgg                                                                                                                             <  2:2986175/148‑1 (MQ=255)
                 ttGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCgg                                                                                                                             >  1:2072038/1‑148 (MQ=255)
                 ttGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGATGCTGGTCTTTTTCgg                                                                                                                             >  2:2039796/1‑148 (MQ=255)
                       cATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATgct                                                                                                                       <  1:1354899/148‑1 (MQ=255)
                       cATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATgct                                                                                                                       <  1:2592089/148‑1 (MQ=255)
                        aTGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATgctg                                                                                                                      <  1:1544985/148‑1 (MQ=255)
                        aTGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATgctg                                                                                                                      <  1:1547804/148‑1 (MQ=255)
                          ggTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATgctggg                                                                                                                    <  2:1603275/148‑1 (MQ=255)
                          ggTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATgctggg                                                                                                                    <  2:1603269/148‑1 (MQ=255)
                          ggTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTGTTTTTGGGGATgctggg                                                                                                                    >  1:1255606/1‑148 (MQ=255)
                               ggCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGctgggctggg                                                                                                               <  1:1725969/148‑1 (MQ=255)
                               ggCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCGGGTCTTTTTCGGCATGctgggctggg                                                                                                               <  1:2831242/148‑1 (MQ=255)
                                 cgcgcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGgcg                                                                                                             <  1:35701/148‑1 (MQ=255)
                                 cgcgcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGGCTAGGGGGGCGGGTCGTTTTCGGCATGCGGGGCTGGgcg                                                                                                             >  1:1726969/1‑148 (MQ=255)
                                  gcgcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGgcgc                                                                                                            <  2:78650/148‑1 (MQ=255)
                                    gcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCTTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCgctg                                                                                                          <  1:542980/148‑1 (MQ=255)
                                    gcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCTTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCgctg                                                                                                          <  1:542970/148‑1 (MQ=255)
                                    gcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCgctg                                                                                                          <  1:2748763/148‑1 (MQ=255)
                                      gcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCgctgct                                                                                                        <  2:1072725/148‑1 (MQ=255)
                                      gcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCgctgct                                                                                                        <  1:841400/148‑1 (MQ=255)
                                       cTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTc                                                                                                       >  1:2278899/1‑148 (MQ=255)
                                         ttACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTcac                                                                                                     >  1:1181832/1‑148 (MQ=255)
                                         ttACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCGGGGCGCTGCTcac                                                                                                     >  1:1859026/1‑148 (MQ=255)
                                           aCCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTAGGGATGCTGGTCTTTTTGGGCATGCTGGGCTGGGCGCTGCTCACAg                                                                                                   >  1:503312/1‑148 (MQ=255)
                                            cccTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGc                                                                                                  >  2:2299189/1‑148 (MQ=255)
                                            cccTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGc                                                                                                  >  2:143376/1‑148 (MQ=255)
                                                 ctgcCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGa                                                                                             <  2:41501/148‑1 (MQ=255)
                                                 ctgcCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGa                                                                                             <  2:41489/148‑1 (MQ=255)
                                                 ctgcCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGGGTTGGGTTGCTGGTGTTTTTCGGCATGCTGGGGTGGGCGCTGCTCACAGCGATGa                                                                                             >  1:2933641/1‑148 (MQ=255)
                                                   gcCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaac                                                                                           <  2:1136126/148‑1 (MQ=255)
                                                        tCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaaccaacc                                                                                      <  1:556930/148‑1 (MQ=255)
                                                        tCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaaccaacc                                                                                      <  1:693368/148‑1 (MQ=255)
                                                        tCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaaccaacc                                                                                      >  2:341089/1‑148 (MQ=255)
                                                           tCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCaa                                                                                   <  2:469048/148‑1 (MQ=255)
                                                            ccGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCaaa                                                                                  <  2:3483339/148‑1 (MQ=255)
                                                            ccGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCaaa                                                                                  <  2:2497031/148‑1 (MQ=255)
                                                            ccGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCaaa                                                                                  >  2:1766340/1‑148 (MQ=255)
                                                               tATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTg                                                                               >  2:2658094/1‑148 (MQ=255)
                                                                aTTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTGTCGGGATGCGGGTGTTTTTCGGCATGGTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACggg                                                                              >  2:642484/1‑148 (MQ=255)
                                                                  tCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTgggg                                                                            >  1:1764617/1‑148 (MQ=255)
                                                                   cccGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGc                                                                           <  1:2641629/148‑1 (MQ=255)
                                                                    ccGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCt                                                                          <  1:3344815/148‑1 (MQ=255)
                                                                      gTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTgg                                                                        <  2:1230129/148‑1 (MQ=255)
                                                                      gTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTgg                                                                        <  2:1230131/148‑1 (MQ=255)
                                                                        gAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGGGGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGGTGGCa                                                                      >  2:331997/1‑148 (MQ=255)
                                                                        gAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCa                                                                      >  1:2090262/1‑148 (MQ=255)
                                                                          aaaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAAt                                                                    >  2:2562007/1‑148 (MQ=255)
                                                                           aaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATg                                                                   >  2:2502718/1‑148 (MQ=255)
                                                                           aaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCGTGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGGGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCaaag                                                                   >  1:574279/1‑146 (MQ=255)
                                                                            aTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGc                                                                  >  2:2667307/1‑148 (MQ=255)
                                                                             tGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTGTAGGGGTGCTGGTCTTTTTAGGGATGCTGGGGTGGGGGCTGCTCACAGCGATGAACCAACCCCAAAGGgggcgggccaaggt                                                                 >  1:1049000/1‑142 (MQ=255)
                                                                             tGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCt                                                                 >  2:1680842/1‑148 (MQ=255)
                                                                               cAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGt                                                               <  2:713805/148‑1 (MQ=255)
                                                                                  aaaGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTGTGGTCGCGGGATGCTGGTCTTTTTCGGCATGCGGGGCGGGGCGCGGCTCACAGCCATGAACCCACCCCAACAGGGGCTGGCCATGCtgtgtg                                                            >  1:399448/1‑148 (MQ=255)
                                                                                   aaGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTgg                                                           <  1:1049170/148‑1 (MQ=255)
                                                                                      tttCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTGTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGt                                                        >  1:2976568/1‑148 (MQ=255)
                                                                                               ccTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTgg                                               <  1:2818559/148‑1 (MQ=255)
                                                                                                cTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGt                                              <  2:1311075/148‑1 (MQ=255)
                                                                                                cTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGt                                              <  2:1310900/148‑1 (MQ=255)
                                                                                                    ccGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTAc                                          <  2:524017/148‑1 (MQ=255)
                                                                                                      gCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTg                                        <  2:2715310/148‑1 (MQ=255)
                                                                                                      gCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTg                                        <  2:2715309/148‑1 (MQ=255)
                                                                                                       cTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTGGAGGGCTGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGa                                       >  2:2023816/1‑148 (MQ=255)
                                                                                                       cTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGTCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGa                                       <  2:3414195/148‑1 (MQ=255)
                                                                                                        tGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTCTCGGGCTGGTGGTCTTTTTGGGCATGGTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGAt                                      >  2:363669/1‑148 (MQ=255)
                                                                                                          aCGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTg                                    <  1:1379040/148‑1 (MQ=255)
                                                                                                          aCGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTg                                    <  1:2796518/148‑1 (MQ=255)
                                                                                                           cGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGa                                   <  2:2261706/148‑1 (MQ=255)
                                                                                                                aaaCCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTg                              <  2:2686656/148‑1 (MQ=255)
                                                                                                                aaaCCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGCTGCGGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGGTTGGCGTCGGCTTTGGTTTACTGATTGAACGTg                              >  2:747961/1‑148 (MQ=255)
                                                                                                                 aaCCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTgc                             <  1:1374113/148‑1 (MQ=255)
                                                                                                                   ccGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTgcgc                           >  2:288357/1‑148 (MQ=255)
                                                                                                                     ggATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCaa                         <  1:2232964/148‑1 (MQ=255)
                                                                                                                       aTCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAAt                       <  1:335276/148‑1 (MQ=255)
                                                                                                                         cAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATTAACCAACCCCAACTGGGGCTGGCAAAGCTGGTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATca                     >  1:3268232/1‑147 (MQ=255)
                                                                                                                          aGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTg                    <  1:3201299/148‑1 (MQ=255)
                                                                                                                           ggCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGc                   <  2:2278899/148‑1 (MQ=255)
                                                                                                                           ggCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACGGGGGCTGGCAAAGCTGTTTGGCGTCGGCTTTTGTTTACTGATTGAACGTGCGCAAATATGc                   >  2:2214080/1‑148 (MQ=255)
                                                                                                                            gcgcGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAAATCTGTTTTGCGTCGGCTTTTGTTTAATTATTTAAACTTCGCAAAtaatct                  >  1:2353953/1‑143 (MQ=255)
                                                                                                                                  cgtcgtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACtt            <  1:1963188/148‑1 (MQ=255)
                                                                                                                                    tcgtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCa          <  1:1884793/148‑1 (MQ=255)
                                                                                                                                       ttttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCg       >  2:1761803/1‑148 (MQ=255)
                                                                                                                                         ttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGtt     <  2:2310432/148‑1 (MQ=255)
                                                                                                                                         ttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGtt     <  1:3248938/148‑1 (MQ=255)
                                                                                                                                         ttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGtt     <  1:2299189/148‑1 (MQ=255)
                                                                                                                                         ttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGtt     <  1:2299093/148‑1 (MQ=255)
                                                                                                                                          tCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTc    <  1:1212866/148‑1 (MQ=255)
                                                                                                                                          tCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTc    <  2:2789265/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCG  >  CP009273/2002210‑2002497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATAT  >  CP009273/2002205‑2002503
                                                                                                                                                     |                                                                                                                                                     
TTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTA                                                                                                                                                       <  SRR19973066.877640/150‑1 (MQ=60)
TTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTA                                                                                                                                                       <  SRR19973066.877633/150‑1 (MQ=60)
TTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTA                                                                                                                                                       <  SRR19973066.2748529/150‑1 (MQ=60)
TTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTA                                                                                                                                                       <  SRR19973066.1754324/150‑1 (MQ=60)
   TTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGG                                                                                                                                                    >  SRR19973066.2292574/1‑150 (MQ=60)
      GCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATG                                                                                                                                                 >  SRR19973066.2609322/1‑150 (MQ=60)
        CATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCTAGGGCTGCT                                                                                                                                               >  SRR19973066.1681449/1‑150 (MQ=60)
        CATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCACGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCT                                                                                                                                               <  SRR19973066.269521/150‑1 (MQ=60)
          TCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTAGTGTAGGGCTGCTGG                                                                                                                                             >  SRR19973066.1259713/1‑150 (MQ=60)
             CCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCT                                                                                                                                          <  SRR19973066.427029/150‑1 (MQ=60)
                   CCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCG                                                                                                                                    <  SRR19973066.794829/150‑1 (MQ=60)
                   CCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCG                                                                                                                                    <  SRR19973066.794604/150‑1 (MQ=60)
                    CATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGG                                                                                                                                   >  SRR19973066.2086373/1‑150 (MQ=60)
                      TTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCA                                                                                                                                 <  SRR19973066.466253/150‑1 (MQ=60)
                            CATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGG                                                                                                                           <  SRR19973066.1364710/150‑1 (MQ=60)
                            CATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGG                                                                                                                           <  SRR19973066.2609650/150‑1 (MQ=60)
                             ATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTGTTTTTGGGGATGCTGGG                                                                                                                          >  SRR19973066.1264648/1‑150 (MQ=60)
                             ATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGG                                                                                                                          <  SRR19973066.1558889/150‑1 (MQ=60)
                             ATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGG                                                                                                                          <  SRR19973066.1556042/150‑1 (MQ=60)
                                    GGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCG                                                                                                                   <  SRR19973066.1738451/150‑1 (MQ=60)
                                    GGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCGGGTCTTTTTCGGCATGCTGGGCTGGGCG                                                                                                                   <  SRR19973066.2850269/150‑1 (MQ=60)
                                    GGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCTAGGGTGGCTGGTCTTTTTAGGCATGCTGGGCTGGGCG                                                                                                                   >  SRR19973066.1739484/1‑150 (MQ=60)
                                    GGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGGCTAGGGGGGCGGGTCGTTTTCGGCATGCGGGGCTGGGCG                                                                                                                   >  SRR19973066.1739457/1‑150 (MQ=60)
                                      CGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCT                                                                                                                 <  SRR19973066.36186/150‑1 (MQ=60)
                                         GCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCTTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCT                                                                                                              <  SRR19973066.546910/150‑1 (MQ=60)
                                         GCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCT                                                                                                              <  SRR19973066.2566118/150‑1 (MQ=60)
                                         GCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCT                                                                                                              <  SRR19973066.2767263/150‑1 (MQ=60)
                                         GCGCTTTACCCTGCTGCCCATCTTCCGTAGTCCCTTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCT                                                                                                              <  SRR19973066.546900/150‑1 (MQ=60)
                                          CGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGTTGGTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTC                                                                                                             >  SRR19973066.2294554/1‑150 (MQ=60)
                                           GCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCA                                                                                                            <  SRR19973066.847513/150‑1 (MQ=60)
                                            CTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTGTAGGGATGCTGGTCTTTTTCGGCCTGCTGGGCGGGGCGCTGCTCAC                                                                                                           >  SRR19973066.54962/1‑150 (MQ=60)
                                            CTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCAC                                                                                                           >  SRR19973066.1190136/1‑150 (MQ=60)
                                            CTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCGGGGCGCTGCTCAC                                                                                                           >  SRR19973066.1872314/1‑150 (MQ=60)
                                              TTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTAGGGATGCTGGTCTTTTTGGGCATGCTGGGCTGGGCGCTGCTCACAG                                                                                                         >  SRR19973066.507050/1‑150 (MQ=60)
                                                ACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCG                                                                                                       >  SRR19973066.2112077/1‑150 (MQ=60)
                                                    TGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGGGTTGGGTTGCTGGTGTTTTTCGGCATGCTGGGGTGGGCGCTGCTCACAGCGATGA                                                                                                   >  SRR19973066.2953162/1‑150 (MQ=60)
                                                             TCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCA                                                                                          <  SRR19973066.560919/150‑1 (MQ=60)
                                                             TCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCA                                                                                          <  SRR19973066.698480/150‑1 (MQ=60)
                                                                    TATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCTTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGG                                                                                   >  SRR19973066.2895835/1‑150 (MQ=60)
                                                                    TATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGG                                                                                   <  SRR19973066.1928122/150‑1 (MQ=60)
                                                                     ATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGG                                                                                  >  SRR19973066.1777272/1‑150 (MQ=60)
                                                                        CCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTG                                                                               <  SRR19973066.2659395/150‑1 (MQ=60)
                                                                         CCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGG                                                                              <  SRR19973066.3367084/150‑1 (MQ=60)
                                                                           GTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCA                                                                            >  SRR19973066.2104666/1‑150 (MQ=60)
                                                                              AAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCGTGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGGGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAAAG                                                                         >  SRR19973066.578327/1‑150 (MQ=60)
                                                                                AATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTGTAGGGGTGCTGGTCTTTTTAGGGATGCTGGGGTGGGGGCTGCTCACAGCGATGAACCAACCCcaaagggggcgggccaaggt                                                                       >  SRR19973066.1056710/1‑130 (MQ=60)
                                                                                 ATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTG                                                                      <  SRR19973066.2302589/150‑1 (MQ=60)
                                                                                  TGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTGTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGT                                                                     >  SRR19973066.2346730/1‑150 (MQ=60)
                                                                                   GCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTT                                                                    <  SRR19973066.826206/150‑1 (MQ=60)
                                                                                    CAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTT                                                                   <  SRR19973066.772598/150‑1 (MQ=60)
                                                                                     AGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTCTAGGGATGCTGGTCTTTTTCGGCATGGTGGGGGGGGCGCTGCGGACAGCGATGAACCAACCCACACTGGGGCTGGCAAAGCTGGTTG                                                                  >  SRR19973066.264724/1‑150 (MQ=60)
                                                                                     AGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTGTGGTCGCGGGATGCTGGTCTTTTTCGGCATGCGGGGCGGGGCGCGGCTCACAGCCATGAACCCACCCCAACAGGGGCTGGCCATGCTGTGTG                                                                  >  SRR19973066.402592/1‑150 (MQ=60)
                                                                                        AAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCG                                                               <  SRR19973066.1056883/150‑1 (MQ=60)
                                                                                         AGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTGTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGT                                                              >  SRR19973066.2996239/1‑150 (MQ=60)
                                                                                          GTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTAGGGATGGGGGTCTTTTTGGGCATGCGGGGGGGGGCGCTGCTCACAGCGATGAACCAACCCAAACAGGGGCgggccaagctggttggggtc                                                             >  SRR19973066.2254619/1‑130 (MQ=60)
                                                                                            TTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGG                                                           <  SRR19973066.3385323/150‑1 (MQ=60)
                                                                                              CTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTGGAGGGGTGGTGGTGTTTTTGGGGATGCGGGGCGGGGCGCTGCTCACAGCGCTGAACCAACCCAAACAGGGGCTGGgcctgcggtgtggggtgggcg                                                         >  SRR19973066.369777/1‑129 (MQ=60)
                                                                                              CTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCT                                                         <  SRR19973066.134879/150‑1 (MQ=60)
                                                                                              CTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCT                                                         <  SRR19973066.144750/150‑1 (MQ=60)
                                                                                                GCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTT                                                       <  SRR19973066.2767878/150‑1 (MQ=60)
                                                                                                GCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTT                                                       <  SRR19973066.1865503/150‑1 (MQ=60)
                                                                                                    CCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTT                                                   <  SRR19973066.2837502/150‑1 (MQ=60)
                                                                                                    CCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTT                                                   <  SRR19973066.2837681/150‑1 (MQ=60)
                                                                                                       CACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTAC                                                <  SRR19973066.3005914/150‑1 (MQ=60)
                                                                                                            CTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGANT                                           <  SRR19973066.1386829/150‑1 (MQ=60)
                                                                                                               ACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAA                                        <  SRR19973066.2815343/150‑1 (MQ=60)
                                                                                                               ACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAA                                        <  SRR19973066.1389013/150‑1 (MQ=60)
                                                                                                                 GCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGGAGGGGTGCTGGTCTTTTTAGGCATGAGGGGCGGGGCGCTGCGCAAACCGAAGAACCAACCCCAACAGGGGCTGACAAAGCTGTTTGTCGTGGGCTTTGGgtttatgatttaaag                                      >  SRR19973066.363627/1‑135 (MQ=19)
                                                                                                                      AACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGC                                 <  SRR19973066.1384053/150‑1 (MQ=60)
                                                                                                                          GGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAAT                             <  SRR19973066.2248376/150‑1 (MQ=60)
                                                                                                                            ATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATTAACCAACCCCAACTGGGGCTGGCAAAGCTGGTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCA                           >  SRR19973066.3289698/1‑150 (MQ=60)
                                                                                                                            ATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCT                           <  SRR19973066.2083884/150‑1 (MQ=60)
                                                                                                                            ATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCT                           <  SRR19973066.337846/150‑1 (MQ=60)
                                                                                                                              CAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTAGGCATTCTGGGCTGGGCGCTGCTCCCACCCAAGAACCAACCCAAACAGGGGCTGGCAAATCTGTTTTGCGTCGGCGTTGGTTTAATGATTTAACATGCGCAAAaaagc                         >  SRR19973066.2662388/1‑145 (MQ=60)
                                                                                                                               AGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCT                        <  SRR19973066.3222550/150‑1 (MQ=60)
                                                                                                                               AGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCT                        <  SRR19973066.3222718/150‑1 (MQ=60)
                                                                                                                               AGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAAATCTGTTTTGCGTCGGCTTTTGTTTAattatttaaacttcgcaaataatct                        >  SRR19973066.2369938/1‑125 (MQ=60)
                                                                                                                                     GGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTT                  <  SRR19973066.1057985/150‑1 (MQ=60)
                                                                                                                                       CGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCA                <  SRR19973066.1977068/150‑1 (MQ=60)
                                                                                                                                         TCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGC              <  SRR19973066.1898236/150‑1 (MQ=60)
                                                                                                                                         TCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGC              <  SRR19973066.555571/150‑1 (MQ=60)
                                                                                                                                              TTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCC         <  SRR19973066.3270347/150‑1 (MQ=60)
                                                                                                                                              TTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCC         <  SRR19973066.2314948/150‑1 (MQ=60)
                                                                                                                                              TTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCC         <  SRR19973066.2314851/150‑1 (MQ=60)
                                                                                                                                               TCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCG        >  SRR19973066.3355682/1‑150 (MQ=60)
                                                                                                                                               TCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCG        <  SRR19973066.1221379/150‑1 (MQ=60)
                                                                                                                                                     AGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATAT  >  SRR19973066.2234965/1‑150 (MQ=60)
                                                                                                                                                     AGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATAT  >  SRR19973066.2235381/1‑150 (MQ=60)
                                                                                                                                                     AGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATAT  <  SRR19973066.343720/150‑1 (MQ=60)
                                                                                                                                                     AGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATAG  >  SRR19973066.1397955/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TTCTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATAT  >  CP009273/2002205‑2002503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑