Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,289,374 C→A 100% V142V (GTG→GTT)  ccmC ← heme exporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,289,3740CA99.0% 616.6 / ‑3.1 191V142V (GTG→GTT) ccmCheme exporter subunit
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (83/106);  total (84/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 193 total aligned reads displayed.

GCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCATCGC  >  CP009273/2289229‑2289517
                                                                                                                                                 |                                                                                                                                               
gcATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATaac                                                                                                                                               <  1:127948/148‑1 (MQ=255)
gcATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATaac                                                                                                                                               >  2:740139/1‑148 (MQ=255)
  aTCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAacac                                                                                                                                             >  1:3449421/1‑148 (MQ=255)
  aTCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAacac                                                                                                                                             <  2:1297480/148‑1 (MQ=255)
   tCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACAcc                                                                                                                                            <  1:895040/148‑1 (MQ=255)
       cgTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCcaca                                                                                                                                        <  2:3171940/148‑1 (MQ=255)
        gTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACAt                                                                                                                                       <  2:2081644/148‑1 (MQ=255)
          tGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATAc                                                                                                                                     >  1:1799464/1‑148 (MQ=255)
            aTCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACaa                                                                                                                                   <  1:749427/148‑1 (MQ=255)
             tCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACaaa                                                                                                                                  >  1:161372/1‑148 (MQ=255)
             tCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACaaa                                                                                                                                  >  2:2753428/1‑148 (MQ=255)
              cccTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACaaaa                                                                                                                                 <  1:467932/148‑1 (MQ=255)
                cTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAAc                                                                                                                               <  1:2586736/148‑1 (MQ=255)
                  gATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAAcag                                                                                                                             >  2:829735/1‑148 (MQ=255)
                   aTGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAAcagc                                                                                                                            >  2:2717990/1‑148 (MQ=255)
                   aTGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAAcagc                                                                                                                            >  2:386038/1‑148 (MQ=255)
                      cAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACagcagc                                                                                                                         <  2:1980610/148‑1 (MQ=255)
                      cAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACagcagc                                                                                                                         >  2:1087504/1‑148 (MQ=255)
                           tgtTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAg                                                                                                                    <  1:2085062/148‑1 (MQ=255)
                           tgtTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAg                                                                                                                    <  1:283127/148‑1 (MQ=255)
                            gtTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACCAAAACAGCAGCACCAGt                                                                                                                   >  2:2464236/1‑148 (MQ=255)
                            gtTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGt                                                                                                                   >  2:2465165/1‑148 (MQ=255)
                             ttCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGtt                                                                                                                  <  1:1097493/148‑1 (MQ=255)
                             ttCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGtt                                                                                                                  <  1:1258230/148‑1 (MQ=255)
                             ttCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGtt                                                                                                                  <  1:3034801/148‑1 (MQ=255)
                             ttCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGtt                                                                                                                  >  2:1635061/1‑148 (MQ=255)
                              tCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTc                                                                                                                 <  2:1552475/148‑1 (MQ=255)
                                 accacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGTATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGATGGCGTGCCACAGGGCAATAACACACACATACAAAAACAGCAGCACCAGTTCAGa                                                                                                              <  1:2397318/148‑1 (MQ=255)
                                   cacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAg                                                                                                            <  2:3047328/148‑1 (MQ=255)
                                    acTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGt                                                                                                           <  2:2806342/148‑1 (MQ=255)
                                     cTCCACTGAGTAATGAATTATCGGGAGATTCACCCGGCCAATCAGCTCCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGAGCCACAGCGCAATAACACCCACATACAAAAACAGCAGGACCAGTTCAGAAGTc                                                                                                          <  2:3470046/148‑1 (MQ=255)
                                          cGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACg                                                                                                     <  2:3273062/148‑1 (MQ=255)
                                           ggAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGt                                                                                                    <  1:1127172/148‑1 (MQ=255)
                                             aGTAATGAATACTCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGc                                                                                                  <  1:2455186/148‑1 (MQ=255)
                                             aGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGc                                                                                                  <  1:835308/148‑1 (MQ=255)
                                             aGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGc                                                                                                  >  1:2592305/1‑148 (MQ=255)
                                                aaTGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATc                                                                                               <  1:306264/148‑1 (MQ=255)
                                                aaTGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATc                                                                                               <  1:306129/148‑1 (MQ=255)
                                                  tGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATccc                                                                                             >  2:1145109/1‑148 (MQ=255)
                                                   gAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCa                                                                                            <  2:2277851/148‑1 (MQ=255)
                                                    aataatCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCAt                                                                                           >  2:1236804/1‑148 (MQ=255)
                                                      taatCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATAc                                                                                         >  2:510364/1‑148 (MQ=255)
                                                       actCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATAcc                                                                                        <  2:3462897/146‑1 (MQ=255)
                                                          cGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACccac                                                                                     <  2:2858611/148‑1 (MQ=255)
                                                           ggCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACccacc                                                                                    <  1:1873125/148‑1 (MQ=255)
                                                              aGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAgg                                                                                 <  1:1800428/148‑1 (MQ=255)
                                                              aGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAgg                                                                                 <  1:201452/148‑1 (MQ=255)
                                                              aGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAgg                                                                                 <  1:1800018/148‑1 (MQ=255)
                                                               gATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGt                                                                                >  2:3412989/1‑148 (MQ=255)
                                                               gATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGt                                                                                >  2:2130957/1‑148 (MQ=255)
                                                                  tCACCACGCCAATCAGCTCCAGGATACCTGCCGCTCGGCCCGCCAGACGGCGGTCGTCGAAGGAGTGCCACAGTTCAATAACTCCCACATACTAAATCATCAGCTCCATTTCAGATGTCAGACGTGCATCCAATACCCACAAGTTgac                                                                             <  2:1769740/148‑3 (MQ=255)
                                                                  tCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGcc                                                                             >  1:1693274/1‑148 (MQ=255)
                                                                  tCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGcc                                                                             <  2:2339392/148‑1 (MQ=255)
                                                                  tCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGcc                                                                             <  2:1927714/148‑1 (MQ=255)
                                                                   caccacGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGccc                                                                            <  2:3081829/148‑1 (MQ=255)
                                                                     ccacGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCca                                                                          >  2:1039072/1‑148 (MQ=255)
                                                                            aaTCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGt                                                                   >  1:3314/1‑148 (MQ=255)
                                                                             aTCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGtt                                                                  >  2:3148141/1‑148 (MQ=255)
                                                                              tCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGttt                                                                 >  2:2342845/1‑148 (MQ=255)
                                                                               cAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCCGGTGCCCCACATCGGtttt                                                                <  2:3396610/148‑1 (MQ=255)
                                                                                aGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTc                                                               <  2:852577/148‑1 (MQ=255)
                                                                                 gCAACAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGAGCCAAAGGGCAATAACACCCAAATACAAAAACAGCAGCACCAGATCAGAAGTCAGACGTGCATCACATACCCACCAGGTTCCCCACATCGGATTTcc                                                              >  2:2967299/1‑148 (MQ=255)
                                                                                  cACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTccc                                                             >  2:947928/1‑148 (MQ=255)
                                                                                     cAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCAt                                                          >  1:1567276/1‑148 (MQ=255)
                                                                                     cAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCAt                                                          >  1:662189/1‑148 (MQ=255)
                                                                                        gATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCa                                                       >  2:478919/1‑148 (MQ=255)
                                                                                          tACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCaga                                                     <  2:855103/148‑1 (MQ=255)
                                                                                           aCCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCagag                                                    >  1:2478481/1‑148 (MQ=255)
                                                                                           aCCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCagag                                                    >  1:3353576/1‑148 (MQ=255)
                                                                                           aCCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCagag                                                    <  1:2516759/148‑1 (MQ=255)
                                                                                           aCCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCagag                                                    >  1:2478770/1‑148 (MQ=255)
                                                                                              tGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCg                                                 <  1:3152362/148‑1 (MQ=255)
                                                                                              tGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCg                                                 >  1:799748/1‑148 (MQ=255)
                                                                                              tGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCg                                                 <  1:455371/148‑1 (MQ=255)
                                                                                                ccGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGt                                               >  2:780122/1‑148 (MQ=255)
                                                                                                  gCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTaa                                             <  1:2209763/148‑1 (MQ=255)
                                                                                                    aCGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAAcc                                           <  1:480997/148‑1 (MQ=255)
                                                                                                    aCGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAAcc                                           <  1:3419154/148‑1 (MQ=255)
                                                                                                    aCGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAAcc                                           >  2:2216726/1‑148 (MQ=255)
                                                                                                     cGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCa                                          >  1:2123974/1‑148 (MQ=255)
                                                                                                          cGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCa                                     <  1:2412985/148‑1 (MQ=255)
                                                                                                              aGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATaa                                 <  1:2678917/148‑1 (MQ=255)
                                                                                                              aGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATaa                                 <  1:2678930/148‑1 (MQ=255)
                                                                                                              aGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATaa                                 >  1:1019189/1‑148 (MQ=255)
                                                                                                               gACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATaaa                                >  1:491162/1‑148 (MQ=255)
                                                                                                               gACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATaaa                                >  1:1212427/1‑148 (MQ=255)
                                                                                                                    cggTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTaa                           <  2:970912/148‑1 (MQ=255)
                                                                                                                     ggTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTaaa                          <  1:3512917/148‑1 (MQ=255)
                                                                                                                       tcgtcgAAGGCGTGCCACAGGGCAATCACACCCCCATACAAAAACAGCAGCACCCGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGGGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGGAAaca                        <  1:64832/148‑1 (MQ=255)
                                                                                                                         gtcgAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACg                      <  2:2253099/148‑1 (MQ=255)
                                                                                                                           cgAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGc                    >  1:2515790/1‑148 (MQ=255)
                                                                                                                           cgAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGc                    <  1:740139/148‑1 (MQ=255)
                                                                                                                             aaGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCAc                  >  2:2573152/1‑148 (MQ=255)
                                                                                                                              aGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCAcc                 >  1:558105/1‑148 (MQ=255)
                                                                                                                                gCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCaa               >  1:2148108/1‑148 (MQ=255)
                                                                                                                                 cGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAAt              >  2:1449948/1‑148 (MQ=255)
                                                                                                                                  gTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATg             <  2:1467441/148‑1 (MQ=255)
                                                                                                                                   tGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATgg            <  2:2550139/148‑1 (MQ=255)
                                                                                                                                       acaGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGc        <  2:54009/148‑1 (MQ=255)
                                                                                                                                          gggCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCAt     >  1:260782/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCATCGC  >  CP009273/2289229‑2289517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCATCGCCG  >  CP009273/2289227‑2289519
                                                                                                                                                   |                                                                                                                                                 
CTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAAC                                                                                                                                                 <  SRR19973066.3304062/150‑1 (MQ=60)
CTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAAC                                                                                                                                                 <  SRR19973066.3302570/150‑1 (MQ=60)
 TGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACA                                                                                                                                                <  SRR19973066.1348490/150‑1 (MQ=60)
  GCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACAC                                                                                                                                               <  SRR19973066.129212/150‑1 (MQ=60)
  GCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACAC                                                                                                                                               >  SRR19973066.3472483/1‑150 (MQ=60)
    ATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGGAGGCGTGCCACAGGGCAATAACACCC                                                                                                                                             >  SRR19973066.823584/1‑150 (MQ=60)
     TCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCA                                                                                                                                            <  SRR19973066.901371/150‑1 (MQ=60)
         CGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGNACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATA                                                                                                                                        >  SRR19973066.1825190/1‑150 (MQ=60)
          GTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATAC                                                                                                                                       <  SRR19973066.874444/150‑1 (MQ=60)
          GTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATAC                                                                                                                                       >  SRR19973066.1812368/1‑150 (MQ=60)
          GTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATAC                                                                                                                                       >  SRR19973066.1812252/1‑150 (MQ=60)
          GTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATAC                                                                                                                                       >  SRR19973066.2429013/1‑150 (MQ=60)
             GATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAA                                                                                                                                    >  SRR19973066.162843/1‑150 (MQ=60)
             GATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAA                                                                                                                                    <  SRR19973066.2443037/150‑1 (MQ=60)
              ATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAA                                                                                                                                   <  SRR19973066.755391/150‑1 (MQ=60)
              ATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAA                                                                                                                                   <  SRR19973066.754986/150‑1 (MQ=60)
                CCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAAC                                                                                                                                 <  SRR19973066.3058004/150‑1 (MQ=60)
                CCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAAC                                                                                                                                 <  SRR19973066.471492/150‑1 (MQ=60)
                CCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAAC                                                                                                                                 <  SRR19973066.1405881/150‑1 (MQ=60)
                  CTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAG                                                                                                                               <  SRR19973066.2604579/150‑1 (MQ=60)
                  CTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAG                                                                                                                               >  SRR19973066.3104939/1‑150 (MQ=60)
                  CTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAG                                                                                                                               <  SRR19973066.2604267/150‑1 (MQ=60)
                        CAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCAC                                                                                                                         >  SRR19973066.3299442/1‑150 (MQ=60)
                           GGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAG                                                                                                                      >  SRR19973066.2567462/1‑150 (MQ=60)
                             TGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTT                                                                                                                    <  SRR19973066.2099442/150‑1 (MQ=60)
                             TGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTT                                                                                                                    <  SRR19973066.285179/150‑1 (MQ=60)
                               TTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCA                                                                                                                  <  SRR19973066.1105470/150‑1 (MQ=60)
                               TTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCA                                                                                                                  <  SRR19973066.3055080/150‑1 (MQ=60)
                               TTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCA                                                                                                                  <  SRR19973066.1267397/150‑1 (MQ=60)
                               TTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCA                                                                                                                  <  SRR19973066.1267301/150‑1 (MQ=60)
                               TTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCA                                                                                                                  <  SRR19973066.1105488/150‑1 (MQ=60)
                                   ACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGTATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGATGGCGTGCCACAGGGCAATAACACACACATACAAAAACAGCAGCACCAGTTCAGAAG                                                                                                              <  SRR19973066.2413480/150‑1 (MQ=60)
                                    CCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGT                                                                                                             >  SRR19973066.3503997/1‑150 (MQ=60)
                                             GGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTTAGACGTGC                                                                                                    >  SRR19973066.2609878/1‑150 (MQ=60)
                                             GGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGC                                                                                                    <  SRR19973066.463407/150‑1 (MQ=60)
                                             GGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGC                                                                                                    >  SRR19973066.2609866/1‑150 (MQ=60)
                                             GGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGC                                                                                                    <  SRR19973066.1135271/150‑1 (MQ=60)
                                               AGTAATGAATACTCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCAT                                                                                                  <  SRR19973066.2471915/150‑1 (MQ=60)
                                               AGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCAT                                                                                                  <  SRR19973066.672121/150‑1 (MQ=60)
                                               AGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCAT                                                                                                  <  SRR19973066.2063136/150‑1 (MQ=60)
                                               AGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCAT                                                                                                  <  SRR19973066.841397/150‑1 (MQ=60)
                                                  AATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCC                                                                                               <  SRR19973066.308462/150‑1 (MQ=60)
                                                  AATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCC                                                                                               <  SRR19973066.578901/150‑1 (MQ=60)
                                                  AATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCC                                                                                               <  SRR19973066.308324/150‑1 (MQ=60)
                                                  AATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCC                                                                                               <  SRR19973066.308461/150‑1 (MQ=60)
                                                        TAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCC                                                                                         >  SRR19973066.964672/1‑150 (MQ=60)
                                                             GGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAG                                                                                    <  SRR19973066.1886500/150‑1 (MQ=60)
                                                                AGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTG                                                                                 <  SRR19973066.1813222/150‑1 (MQ=60)
                                                                AGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTG                                                                                 <  SRR19973066.1813221/150‑1 (MQ=60)
                                                                AGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTG                                                                                 <  SRR19973066.1812811/150‑1 (MQ=60)
                                                                AGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTG                                                                                 <  SRR19973066.203157/150‑1 (MQ=60)
                                                                AGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTG                                                                                 >  SRR19973066.1265319/1‑150 (MQ=60)
                                                                AGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTG                                                                                 <  SRR19973066.3413142/150‑1 (MQ=60)
                                                                  ATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCC                                                                               >  SRR19973066.1705586/1‑150 (MQ=60)
                                                                        CACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACAT                                                                         >  SRR19973066.2303912/1‑150 (MQ=60)
                                                                            CCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGT                                                                     >  SRR19973066.3366/1‑150 (MQ=60)
                                                                              AATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTT                                                                   >  SRR19973066.2152331/1‑150 (MQ=60)
                                                                              AATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTT                                                                   >  SRR19973066.1756792/1‑150 (MQ=60)
                                                                               ataagcaactgtATACCTGCCGCACGGCCCGCCAGACTGCGGTCGTCGATGGCGTGACAAAGGGCAATAACACACAAATACAACAACAGCAGCACCAGTTCAGAAGTAAGACTTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTT                                                                  <  SRR19973066.1361652/138‑1 (MQ=60)
                                                                               ATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTT                                                                  >  SRR19973066.2476660/1‑150 (MQ=60)
                                                                                     ACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCAT                                                            >  SRR19973066.667025/1‑150 (MQ=60)
                                                                                     ACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCAT                                                            >  SRR19973066.667021/1‑150 (MQ=60)
                                                                                     ACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCAT                                                            >  SRR19973066.1578566/1‑150 (MQ=60)
                                                                                     ACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCAT                                                            <  SRR19973066.2628805/150‑1 (MQ=60)
                                                                                           ATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAG                                                      >  SRR19973066.2495718/1‑150 (MQ=60)
                                                                                           ATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAG                                                      >  SRR19973066.3375921/1‑150 (MQ=60)
                                                                                           ATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAG                                                      >  SRR19973066.2495394/1‑150 (MQ=60)
                                                                                           ATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAG                                                      >  SRR19973066.2495517/1‑150 (MQ=60)
                                                                                           ATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAG                                                      >  SRR19973066.2495683/1‑150 (MQ=60)
                                                                                             ACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCC                                                    <  SRR19973066.2533847/150‑1 (MQ=60)
                                                                                             ACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCC                                                    <  SRR19973066.2533880/150‑1 (MQ=60)
                                                                                              CCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCG                                                   >  SRR19973066.805620/1‑150 (MQ=60)
                                                                                                TGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGT                                                 <  SRR19973066.3173376/150‑1 (MQ=60)
                                                                                                TGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGT                                                 <  SRR19973066.458871/150‑1 (MQ=60)
                                                                                                    GCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACC                                             <  SRR19973066.2225070/150‑1 (MQ=60)
                                                                                                    GCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACC                                             <  SRR19973066.2225056/150‑1 (MQ=60)
                                                                                                     CACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCA                                            >  SRR19973066.2138678/1‑150 (MQ=60)
                                                                                                      ACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAG                                           <  SRR19973066.3442043/150‑1 (MQ=60)
                                                                                                      ACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAG                                           <  SRR19973066.482530/150‑1 (MQ=60)
                                                                                                      ACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAG                                           <  SRR19973066.484617/150‑1 (MQ=60)
                                                                                                      ACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAG                                           <  SRR19973066.3442027/150‑1 (MQ=60)
                                                                                                        GGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGG                                         <  SRR19973066.1200701/150‑1 (MQ=60)
                                                                                                         GCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGC                                        <  SRR19973066.1600712/150‑1 (MQ=60)
                                                                                                            CGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAAT                                     <  SRR19973066.2429329/150‑1 (MQ=60)
                                                                                                              CCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAA                                   >  SRR19973066.1026688/1‑150 (MQ=60)
                                                                                                               CAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAA                                  >  SRR19973066.1220933/1‑150 (MQ=60)
                                                                                                               CAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAA                                  >  SRR19973066.494839/1‑150 (MQ=60)
                                                                                                                AGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAG                                 <  SRR19973066.2696797/150‑1 (MQ=60)
                                                                                                                AGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAG                                 <  SRR19973066.2696810/150‑1 (MQ=60)
                                                                                                                       GGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACA                          <  SRR19973066.3536317/150‑1 (MQ=60)
                                                                                                                         TCGTCGAAGGCGTGCCACAGGGCAATCACACCCCCATACAAAAACAGCAGCACCCGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGGGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGGAAACACC                        <  SRR19973066.65624/150‑1 (MQ=60)
                                                                                                                          CGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGG                       >  SRR19973066.1917885/1‑150 (MQ=60)
                                                                                                                           GTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGNC                      <  SRR19973066.3344574/150‑1 (MQ=60)
                                                                                                                           GTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGC                      >  SRR19973066.2532906/1‑150 (MQ=60)
                                                                                                                             CGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCAC                    <  SRR19973066.745636/150‑1 (MQ=60)
                                                                                                                              GAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACC                   >  SRR19973066.562098/1‑150 (MQ=60)
                                                                                                                              GAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACC                   >  SRR19973066.562084/1‑150 (MQ=60)
                                                                                                                                AGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAA                 >  SRR19973066.2163016/1‑150 (MQ=60)
                                                                                                                                  GCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATG               <  SRR19973066.389096/150‑1 (MQ=60)
                                                                                                                                        CACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCC         >  SRR19973066.2931246/1‑150 (MQ=60)
                                                                                                                                         ACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCA        >  SRR19973066.2698897/1‑150 (MQ=60)
                                                                                                                                          CAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCAT       >  SRR19973066.262742/1‑150 (MQ=60)
                                                                                                                                          CAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCAT       >  SRR19973066.1695982/1‑150 (MQ=60)
                                                                                                                                             GGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCATCGC    >  SRR19973066.753604/1‑150 (MQ=60)
                                                                                                                                               CAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCATCGCCG  >  SRR19973066.982901/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                 
CTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCATCGCCG  >  CP009273/2289227‑2289519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑