Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,844,024 T→A 48.3% C7S (TGC→AGC)  hypA → protein involved in nickel insertion into hydrogenases 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,844,0240TA48.3% 20.9 / inf 207C7S (TGC→AGC) hypAprotein involved in nickel insertion into hydrogenases 3
Reads supporting (aligned to +/- strand):  ref base T (47/60);  new base A (49/51);  total (96/111)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

TTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCTTTTGTTTTGATCTGGTTTGCCGCGGCAGC  >  CP009273/2843877‑2844170
                                                                                                                                                   |                                                                                                                                                  
ttAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCctct                                                                                                                                                    >  1:2455213/1‑148 (MQ=255)
ttAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCctct                                                                                                                                                    >  1:665757/1‑148 (MQ=255)
    gCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCa                                                                                                                                                >  2:3404229/1‑148 (MQ=255)
     cTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGTCaa                                                                                                                                               <  1:1016045/148‑1 (MQ=255)
     cTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCaa                                                                                                                                               <  2:573300/148‑1 (MQ=255)
        aaGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACgg                                                                                                                                            >  1:1803653/1‑148 (MQ=255)
         aGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACggg                                                                                                                                           >  2:3533235/1‑148 (MQ=255)
         aGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACggg                                                                                                                                           >  2:643968/1‑148 (MQ=255)
                  tcgtcgCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAAt                                                                                                                                  >  1:1331003/1‑148 (MQ=255)
                     tcgCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGa                                                                                                                               >  2:3151182/1‑148 (MQ=255)
                        ccGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCg                                                                                                                            <  1:1271098/148‑1 (MQ=255)
                           tgtCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAAc                                                                                                                         >  2:1567226/1‑148 (MQ=255)
                            gtCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAAca                                                                                                                        >  2:3158858/1‑148 (MQ=255)
                              cGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAAcagc                                                                                                                      <  2:2345143/148‑1 (MQ=255)
                              cGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAAcagc                                                                                                                      <  1:3338326/148‑1 (MQ=255)
                              cGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAAcagc                                                                                                                      >  2:733744/1‑148 (MQ=255)
                               gACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAAcagca                                                                                                                     <  2:3461534/148‑1 (MQ=255)
                                 cGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAgg                                                                                                                   >  1:2612480/1‑148 (MQ=255)
                                 cGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAgg                                                                                                                   >  1:997165/1‑148 (MQ=255)
                                 cGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAgg                                                                                                                   >  2:2462507/1‑148 (MQ=255)
                                 cGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAgg                                                                                                                   <  2:817656/148‑1 (MQ=255)
                                 cGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAgg                                                                                                                   <  2:1944692/148‑1 (MQ=255)
                                   tgtCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGcc                                                                                                                 >  2:2590886/1‑148 (MQ=255)
                                    gtCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCg                                                                                                                >  1:2309177/1‑148 (MQ=255)
                                        tttCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTCATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCaaa                                                                                                            >  1:3483841/1‑148 (MQ=255)
                                        tttCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCaaa                                                                                                            >  2:2227861/1‑148 (MQ=255)
                                        tttCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCaaa                                                                                                            >  1:1404524/1‑148 (MQ=255)
                                          tCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAac                                                                                                          <  1:3137061/148‑1 (MQ=255)
                                              catcatCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACgg                                                                                                      >  2:467608/1‑148 (MQ=255)
                                              catcatCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACgg                                                                                                      <  1:1705668/148‑1 (MQ=255)
                                              catcatCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACgg                                                                                                      >  2:2727703/1‑148 (MQ=255)
                                                tcatcGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGgcg                                                                                                    >  2:1489633/1‑148 (MQ=255)
                                                    cGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCaaa                                                                                                <  1:638506/148‑1 (MQ=255)
                                                     gACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCaaaa                                                                                               <  1:279364/148‑1 (MQ=255)
                                                      aCATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAAc                                                                                              >  1:2963595/1‑148 (MQ=255)
                                                      aCATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAAc                                                                                              >  1:17489/1‑148 (MQ=255)
                                                       cATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAAcg                                                                                             >  1:2367270/1‑148 (MQ=255)
                                                           attCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTa                                                                                         <  2:338858/148‑1 (MQ=255)
                                                                ccGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTgg                                                                                    >  1:1303417/1‑148 (MQ=255)
                                                                ccGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTgg                                                                                    >  2:2038239/1‑148 (MQ=255)
                                                                   cAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGt                                                                                 <  2:2816660/148‑1 (MQ=255)
                                                                    aGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTc                                                                                <  1:2152032/148‑1 (MQ=255)
                                                                    aGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTc                                                                                <  1:1685461/148‑1 (MQ=255)
                                                                     gggATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCt                                                                               <  1:8827/148‑1 (MQ=255)
                                                                     gggATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCt                                                                               <  1:1853645/148‑1 (MQ=255)
                                                                      ggATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTg                                                                              >  1:808669/1‑148 (MQ=255)
                                                                       gATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTgg                                                                             >  2:3541544/1‑148 (MQ=255)
                                                                        aTAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGc                                                                            <  1:3404229/148‑1 (MQ=255)
                                                                            tCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCaa                                                                        >  2:3029097/1‑148 (MQ=255)
                                                                            tCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCaa                                                                        >  2:1243812/1‑148 (MQ=255)
                                                                             cAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCaac                                                                       >  2:3244412/1‑147 (MQ=255)
                                                                             cAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCaaa                                                                       >  2:863801/1‑148 (MQ=255)
                                                                             cAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCaaa                                                                       <  1:3468085/148‑1 (MQ=255)
                                                                              aaCACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCaaaa                                                                      <  2:2455213/148‑1 (MQ=255)
                                                                              aaCACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCaaaa                                                                      <  2:665945/148‑1 (MQ=255)
                                                                                 acTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTg                                                                   <  2:3339246/148‑1 (MQ=255)
                                                                                    ggCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGgcg                                                                <  2:2504993/148‑1 (MQ=255)
                                                                                     gCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGgcgc                                                               <  1:2681577/148‑1 (MQ=255)
                                                                                     gCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGgcgc                                                               <  2:1888463/148‑1 (MQ=255)
                                                                                      cacaATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCa                                                              <  1:683208/148‑1 (MQ=255)
                                                                                       acaATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCAt                                                             >  1:866584/1‑148 (MQ=255)
                                                                                        caATTATTGCTTGTAGCTGGCAAGAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAAGTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCAtt                                                            <  2:2308418/148‑1 (MQ=255)
                                                                                         aaTTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCAttt                                                           >  1:1204734/1‑148 (MQ=255)
                                                                                           ttattGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTc                                                         >  1:2641843/1‑148 (MQ=255)
                                                                                           ttattGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTc                                                         <  1:3029097/148‑1 (MQ=255)
                                                                                           ttattGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTc                                                         <  2:1331003/148‑1 (MQ=255)
                                                                                            tattGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCt                                                        >  2:3238947/1‑148 (MQ=255)
                                                                                              ttGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTtg                                                      >  1:2411050/1‑148 (MQ=255)
                                                                                              ttGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTtg                                                      <  1:409114/148‑1 (MQ=255)
                                                                                               tGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTtgt                                                     <  1:3029124/148‑1 (MQ=255)
                                                                                                 cTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGATATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTgtgt                                                   <  2:37543/148‑1 (MQ=255)
                                                                                                 cTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTgtgt                                                   <  1:1965697/148‑1 (MQ=255)
                                                                                                   tGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCg                                                 >  2:409995/1‑148 (MQ=255)
                                                                                                           gCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGc                                         <  1:2549604/148‑1 (MQ=255)
                                                                                                           gCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGc                                         >  2:3431006/1‑148 (MQ=255)
                                                                                                            cAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGct                                        <  1:3191274/148‑1 (MQ=255)
                                                                                                            cAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGct                                        <  1:2717163/148‑1 (MQ=255)
                                                                                                            cAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGct                                        <  1:1450369/148‑1 (MQ=255)
                                                                                                            cAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGct                                        <  2:211792/148‑1 (MQ=255)
                                                                                                            cAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGct                                        <  2:1771351/148‑1 (MQ=255)
                                                                                                            aaaTAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGct                                        <  1:3223574/147‑1 (MQ=255)
                                                                                                              aTAGTTAATGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGctct                                      <  1:385854/148‑1 (MQ=255)
                                                                                                                aGTTACTGGGAGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGctctct                                    <  2:2312465/148‑1 (MQ=255)
                                                                                                                  ttAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTg                                  <  2:1573360/148‑1 (MQ=255)
                                                                                                                     aTGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCt                               >  1:1053127/1‑148 (MQ=255)
                                                                                                                      tGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCtt                              >  1:2334701/1‑148 (MQ=255)
                                                                                                                      tGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCtt                              >  1:2902253/1‑148 (MQ=255)
                                                                                                                        ggAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCtttt                            <  2:1112476/148‑1 (MQ=255)
                                                                                                                         gAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCttttg                           <  2:14424/148‑1 (MQ=255)
                                                                                                                          aGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCttttgt                          <  2:1881999/148‑1 (MQ=255)
                                                                                                                          aGGCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCttttgt                          <  1:733744/148‑1 (MQ=255)
                                                                                                                            gCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCttttgttt                        >  2:1206685/1‑148 (MQ=255)
                                                                                                                            gCGATATGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCttttgttt                        >  2:2836251/1‑148 (MQ=255)
                                                                                                                                 atGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCTTTTGTTTTGATc                   <  2:537869/148‑1 (MQ=255)
                                                                                                                                 atGCACGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCTTTTGTTTTGATc                   >  2:2959528/1‑148 (MQ=255)
                                                                                                                                      cGAAATAACCCTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCTTTTGTTTTGATCTGGtt              <  2:2055278/148‑1 (MQ=255)
                                                                                                                                        aaaTAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCTTTTGTTTTGATCTGGTTTg            <  1:2590886/148‑1 (MQ=255)
                                                                                                                                              cccTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCTTTTGTTTTGATCTGGTTTGCCGCgg      >  1:1667759/1‑148 (MQ=255)
                                                                                                                                              cccTCAGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCTTTTGTTTTGATCTGGTTTGCCGCgg      >  1:523669/1‑148 (MQ=255)
                                                                                                                                                  ctGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCTTTTGTTTTGATCTGGTTTGCCGCGGCAGc  >  1:1135463/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGCAATAGTTAATGGGAGGCGATATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAACTGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCTTTTGTTTTGATCTGGTTTGCCGCGGCAGC  >  CP009273/2843877‑2844170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A