Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,783,857 A→T 44.9% I283I (ATT→ATA)  tdh ← L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,783,8570AT44.9% 76.7 / 315.4 216I283I (ATT→ATA) tdhL‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (49/70);  new base T (48/49);  total (97/119)
Fisher's exact test for biased strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 222 total aligned reads displayed.

CTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCG  >  CP009273/3783710‑3784001
                                                                                                                                                   |                                                                                                                                                
cTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTa                                                                                                                                                  <  1:3194508/148‑1 (MQ=255)
   gCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATg                                                                                                                                               >  1:2995545/1‑148 (MQ=255)
   gCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATg                                                                                                                                               >  1:1843870/1‑148 (MQ=255)
       gAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACa                                                                                                                                           >  2:2088732/1‑148 (MQ=255)
         aCGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAg                                                                                                                                         >  1:2376932/1‑148 (MQ=255)
           gCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGcc                                                                                                                                       <  2:3397353/148‑1 (MQ=255)
            cATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCt                                                                                                                                      <  2:3442158/148‑1 (MQ=255)
             aTAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCtt                                                                                                                                     <  2:167814/148‑1 (MQ=255)
             aTAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCtt                                                                                                                                     <  2:1809654/148‑1 (MQ=255)
              tAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCttt                                                                                                                                    >  2:2124274/1‑148 (MQ=255)
                gCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTaa                                                                                                                                  <  1:1944544/148‑1 (MQ=255)
                 cGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGTTGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTaaa                                                                                                                                 <  2:905909/148‑1 (MQ=255)
                 cGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTaaa                                                                                                                                 <  2:2952618/148‑1 (MQ=255)
                  gaaaaaGCCCTTGTGTAAATCATCTATATAGAACCGTTGGGTATTTATGGGCTAAATATCGCGGCCAGACTTAATCAGCGCCGCCATCTTGTACCTGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAg                                                                                                                                <  1:17723/145‑1 (MQ=255)
                  gTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAg                                                                                                                                <  1:2613856/148‑1 (MQ=255)
                  gTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAg                                                                                                                                <  2:2224149/148‑1 (MQ=255)
                  gTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAg                                                                                                                                <  2:2224646/148‑1 (MQ=255)
                   tCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGa                                                                                                                               <  2:852583/148‑1 (MQ=255)
                   tCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGa                                                                                                                               <  2:1136368/148‑1 (MQ=255)
                    aaaaGCCCTTCTGTAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGAt                                                                                                                              <  1:731825/147‑1 (MQ=255)
                     aaaGCCCTTGTGTAAATCTTCGATAGAGAAACTATGGGTATTGATGGGCGAAAGATCGAGGCCAGACTTAATCAGCGCCGCCATCTTGTACCTGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATc                                                                                                                             <  1:1309194/148‑1 (MQ=255)
                     aaaGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAAACAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATc                                                                                                                             <  2:2276803/148‑1 (MQ=255)
                      aaGCCCTTATGTAAATCATCGATAGAGAAACGTTGGGTAATGATCGGCTAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCTGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCa                                                                                                                            <  1:2116763/148‑1 (MQ=255)
                        gCCCTTCTGGAAATAATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACt                                                                                                                          <  2:2547602/148‑1 (MQ=255)
                         cccTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACtt                                                                                                                         <  1:3335662/148‑1 (MQ=255)
                         ccattCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTAAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACtt                                                                                                                         <  1:3308258/145‑1 (MQ=255)
                               tGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTcc                                                                                                                   <  2:1228674/148‑1 (MQ=255)
                                      catcGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGAt                                                                                                            <  2:3228113/148‑1 (MQ=255)
                                       atcGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATa                                                                                                           <  2:1734827/148‑1 (MQ=255)
                                       atcGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATa                                                                                                           <  2:3263974/148‑1 (MQ=255)
                                       atcGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATATTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATa                                                                                                           <  2:1278607/148‑1 (MQ=255)
                                        tctaTAGATAAACTATGGGTTATGATGGTCGAAAGATCGTGTCCAGAGTGAATCTGCGCCGCCATCTTGTACCTGTTTTAAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAg                                                                                                          <  1:2163654/145‑1 (MQ=255)
                                        tcGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGCCCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAg                                                                                                          <  2:3523907/148‑1 (MQ=255)
                                        tcGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAg                                                                                                          <  2:2497017/148‑1 (MQ=255)
                                        tcGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAg                                                                                                          <  1:1115167/148‑1 (MQ=255)
                                          gATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGAc                                                                                                        <  2:2330576/148‑1 (MQ=255)
                                            tAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACat                                                                                                      >  2:696301/1‑148 (MQ=255)
                                            tAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACat                                                                                                      >  2:1601545/1‑148 (MQ=255)
                                             agagAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACata                                                                                                     >  1:3432442/1‑148 (MQ=255)
                                              gagaAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACatat                                                                                                    <  2:455469/148‑1 (MQ=255)
                                               agaAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATc                                                                                                   <  1:1059872/148‑1 (MQ=255)
                                                gaAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCa                                                                                                  >  1:2057986/1‑148 (MQ=255)
                                                caaaCGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCa                                                                                                  <  1:966703/147‑1 (MQ=255)
                                                    cGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGAcg                                                                                              >  2:1942917/1‑148 (MQ=255)
                                                      aTGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGAcggc                                                                                            <  2:1890559/148‑1 (MQ=255)
                                                       tGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGAcggcg                                                                                           >  1:2789351/1‑148 (MQ=255)
                                                         gttAATGATCGGCGAATGATCGAGGCCAGACTTATTCATCGACGCAATTTTTTACCATGTTTAATACATCTCACGACAGTAAATACCTTTAATTAACAAGACTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGa                                                                                         <  1:1949213/146‑1 (MQ=255)
                                                          gTATTGATCGGCTAAAGATCGTGGCATGATTGAATCAGCGCCGCCTTCTTGTACCAGTTTTCAAACTTCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGaa                                                                                        <  1:664008/148‑1 (MQ=255)
                                                          gTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGaa                                                                                        <  1:1387528/148‑1 (MQ=255)
                                                           tAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAAt                                                                                       >  1:3451361/1‑148 (MQ=255)
                                                            aaTGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATa                                                                                      >  2:1656592/1‑148 (MQ=255)
                                                               gATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATAccc                                                                                   >  2:2527309/1‑148 (MQ=255)
                                                                aTCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCa                                                                                  >  2:3316702/1‑148 (MQ=255)
                                                                 tCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAg                                                                                 >  2:3332971/1‑148 (MQ=255)
                                                                  cGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGc                                                                                >  1:1185936/1‑148 (MQ=255)
                                                                    gCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCAt                                                                              <  2:1047255/148‑1 (MQ=255)
                                                                       aaaGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGc                                                                           >  2:3029845/1‑148 (MQ=255)
                                                                         aGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCaa                                                                         <  2:1492718/148‑1 (MQ=255)
                                                                         aGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCaa                                                                         >  1:1442327/1‑148 (MQ=255)
                                                                          gATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAAt                                                                        >  1:1513049/1‑148 (MQ=255)
                                                                               aGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGgc                                                                   <  1:1053227/148‑1 (MQ=255)
                                                                                      aCTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTg                                                            <  1:1886831/148‑1 (MQ=255)
                                                                                      aCTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTg                                                            >  2:1089231/1‑148 (MQ=255)
                                                                                      aCTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTg                                                            >  2:1087195/1‑148 (MQ=255)
                                                                                      aCTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTg                                                            <  1:82732/148‑1 (MQ=255)
                                                                                      aCTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTg                                                            <  1:3353010/148‑1 (MQ=255)
                                                                                        tGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACAGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGAt                                                          >  1:497059/1‑148 (MQ=255)
                                                                                         gAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGAtt                                                         <  1:2157322/148‑1 (MQ=255)
                                                                                            tCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAATTACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCACTCGATAGACATATCAGACCGCGGAATACCCAGCAGCGCCAAACGGCAGCCGTGATTCag                                                      >  1:121213/1‑147 (MQ=255)
                                                                                            tCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTAACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCAt                                                      >  1:819518/1‑148 (MQ=255)
                                                                                             cAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGTCCGCCGTGATTCATg                                                     >  1:3253900/1‑148 (MQ=255)
                                                                                               gcgcCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGgt                                                   <  1:246709/148‑1 (MQ=255)
                                                                                                cgccgcCATATTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGgc                                                                   >  2:606711/1‑131 (MQ=255)
                                                                                                cgccgcCATATTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGgc                                                                   <  1:606711/131‑1 (MQ=255)
                                                                                                 gccgccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGgtgt                                                 <  2:2376932/148‑1 (MQ=255)
                                                                                                 gccgccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGgtgt                                                 <  2:425788/148‑1 (MQ=255)
                                                                                                    gccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATGCATGGTGTCaa                                              >  2:3240654/1‑148 (MQ=255)
                                                                                                      cATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGc                                            <  2:1815436/148‑1 (MQ=255)
                                                                                                        tCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCAt                                          >  1:555423/1‑148 (MQ=255)
                                                                                                        tCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCAt                                          >  2:2594072/1‑148 (MQ=255)
                                                                                                         cTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATg                                         >  2:1503698/1‑148 (MQ=255)
                                                                                                          ttGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATgg                                        <  1:2574093/148‑1 (MQ=255)
                                                                                                            gTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTa                                      <  2:2907386/148‑1 (MQ=255)
                                                                                                                cAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGaa                                  <  1:75595/148‑1 (MQ=255)
                                                                                                                cAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGaa                                  <  1:1744338/148‑1 (MQ=255)
                                                                                                                     ttCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCt                             <  2:3358325/148‑1 (MQ=255)
                                                                                                                     ttCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCt                             <  1:2914342/148‑1 (MQ=255)
                                                                                                                     ttCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCt                             >  1:416939/1‑148 (MQ=255)
                                                                                                                      tCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTg                            <  1:363530/148‑1 (MQ=255)
                                                                                                                         aaCATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTggcg                         >  1:470071/1‑148 (MQ=255)
                                                                                                                          aCATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTCCGAAACGCTggcgg                        >  2:1510684/1‑148 (MQ=255)
                                                                                                                           cATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTggcggc                       >  2:51890/1‑148 (MQ=255)
                                                                                                                                cACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCAcc                  >  2:154408/1‑148 (MQ=255)
                                                                                                                                cACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCAcc                  >  2:1956180/1‑148 (MQ=255)
                                                                                                                                    aCCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGAc              >  1:965346/1‑148 (MQ=255)
                                                                                                                                      cGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACAt            >  2:2412117/1‑148 (MQ=255)
                                                                                                                                      cGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACAt            >  1:1941973/1‑148 (MQ=255)
                                                                                                                                          aaTACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTcc        <  1:2245548/148‑1 (MQ=255)
                                                                                                                                            tACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAg      >  1:27421/1‑148 (MQ=255)
                                                                                                                                                ttttATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCg  <  1:1799229/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCG  >  CP009273/3783710‑3784001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A