Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,861,553 C→A 100% G373C (GGC→TGC)  yidR ← DUF3748 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,861,5530CA95.3% 635.9 / 20.7 214G373C (GGC→TGC) yidRDUF3748 family protein
Reads supporting (aligned to +/- strand):  ref base C (7/3);  new base A (102/102);  total (109/105)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 215 total aligned reads displayed.

CCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCAC  >  CP009273/3861409‑3861698
                                                                                                                                                |                                                                                                                                                 
ccTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCt                                                                                                                                                >  1:2137011/1‑148 (MQ=255)
ccTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCt                                                                                                                                                <  1:1990616/148‑1 (MQ=255)
  tGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCttt                                                                                                                                              >  2:648480/1‑148 (MQ=255)
   gATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTg                                                                                                                                             >  1:1141485/1‑148 (MQ=255)
    aTAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTgc                                                                                                                                            >  2:3199590/1‑148 (MQ=255)
    aTAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTgc                                                                                                                                            <  2:2526729/148‑1 (MQ=255)
     tAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTgcg                                                                                                                                           <  2:620484/148‑1 (MQ=255)
       aCTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGACTTGGCGTGGTGTTCGGTTAAAGACTCAACCTCGCAGCTTTgcgcc                                                                                                                                         >  2:2408175/1‑147 (MQ=255)
        cTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCAt                                                                                                                                        >  2:3552279/1‑148 (MQ=255)
        cTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCAt                                                                                                                                        >  1:248385/1‑148 (MQ=255)
             ttAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATTGGca                                                                                                                                   >  1:2393650/1‑148 (MQ=255)
             ttAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGca                                                                                                                                   >  1:2663611/1‑148 (MQ=255)
                gCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACaa                                                                                                                                >  2:877775/1‑148 (MQ=255)
                 cGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAg                                                                                                                               >  1:1174136/1‑148 (MQ=255)
                  gATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGc                                                                                                                              <  1:843150/148‑1 (MQ=255)
                  gATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGc                                                                                                                              <  1:201521/148‑1 (MQ=255)
                  gATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGc                                                                                                                              >  1:1623709/1‑148 (MQ=255)
                  gATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGc                                                                                                                              >  1:2201010/1‑148 (MQ=255)
                     cAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGTCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAt                                                                                                                           >  1:3063556/1‑148 (MQ=255)
                     cAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAt                                                                                                                           >  2:1608323/1‑148 (MQ=255)
                     cAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAt                                                                                                                           >  2:2851096/1‑148 (MQ=255)
                        tttCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCg                                                                                                                        <  1:1131701/148‑1 (MQ=255)
                        tttCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCg                                                                                                                        >  2:2949323/1‑148 (MQ=255)
                          tCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGAt                                                                                                                      <  2:1462232/148‑1 (MQ=255)
                                aTCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCa                                                                                                                >  1:1580604/1‑148 (MQ=255)
                                   cacaGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGca                                                                                                             >  1:3444460/1‑148 (MQ=255)
                                       gCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACaaa                                                                                                         >  2:2884439/1‑148 (MQ=255)
                                         tGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGc                                                                                                       <  2:1298369/148‑1 (MQ=255)
                                         tGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGc                                                                                                       <  2:431173/148‑1 (MQ=255)
                                          ggCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGcc                                                                                                      >  1:237201/1‑148 (MQ=255)
                                          ggCCGCCTTCCATCCACGCCAACCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGcc                                                                                                      <  1:573540/148‑1 (MQ=255)
                                             cgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCaa                                                                                                   >  1:85513/1‑148 (MQ=255)
                                              gccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCacc                                                                                                  <  2:1959362/148‑3 (MQ=255)
                                              gccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAAc                                                                                                  >  2:1151742/1‑148 (MQ=255)
                                              gccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAAc                                                                                                  >  2:1998850/1‑148 (MQ=255)
                                              gccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAAc                                                                                                  >  2:855391/1‑148 (MQ=255)
                                               ccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAAcc                                                                                                 >  2:3379951/1‑148 (MQ=255)
                                                cTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCa                                                                                                <  2:2961289/148‑1 (MQ=255)
                                                cTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCa                                                                                                >  1:3338269/1‑148 (MQ=255)
                                                cTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCa                                                                                                >  2:1537947/1‑148 (MQ=255)
                                                  tccatccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCAtt                                                                                              <  2:2742323/148‑1 (MQ=255)
                                                   ccatccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATtc                                                                                             >  2:1563255/1‑148 (MQ=255)
                                                      tccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTcc                                                                                          <  2:2379783/148‑1 (MQ=255)
                                                      tccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTcc                                                                                          <  2:1055687/148‑1 (MQ=255)
                                                       ccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCt                                                                                         >  2:3072756/1‑148 (MQ=255)
                                                         aCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGa                                                                                       >  1:3152369/1‑148 (MQ=255)
                                                            ccagccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACgg                                                                                    <  1:1273353/148‑1 (MQ=255)
                                                            ccagccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACgg                                                                                    <  1:1910224/148‑1 (MQ=255)
                                                            ccagccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTGTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACgg                                                                                    <  1:35266/148‑1 (MQ=255)
                                                              agccaCTGACCATCCGGCGAGAAGACCACGGCGTCCTCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGAGCATGGGCACACGCACTTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGAt                                                                                  >  1:2728864/1‑148 (MQ=255)
                                                               gccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAATGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTAGCCAGCACAAAGCCCAACCATTCTCCTGACGGATg                                                                                 <  1:2645247/148‑1 (MQ=255)
                                                               gccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATg                                                                                 >  1:972968/1‑148 (MQ=255)
                                                               gccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATg                                                                                 <  2:2645724/148‑1 (MQ=255)
                                                                    tGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGt                                                                            <  1:3067168/148‑1 (MQ=255)
                                                                          tCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATg                                                                      >  1:485620/1‑148 (MQ=255)
                                                                          tCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATg                                                                      >  1:1262185/1‑148 (MQ=255)
                                                                           ccGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGc                                                                     >  1:884036/1‑148 (MQ=255)
                                                                           ccGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGc                                                                     <  2:305044/148‑1 (MQ=255)
                                                                           ccGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGc                                                                     <  2:1976328/148‑1 (MQ=255)
                                                                            cGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCa                                                                    >  1:1967574/1‑148 (MQ=255)
                                                                             ggCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAg                                                                   <  2:573130/148‑1 (MQ=255)
                                                                              gCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGa                                                                  <  1:1438259/148‑1 (MQ=255)
                                                                                 agaagaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTg                                                               >  2:706724/1‑148 (MQ=255)
                                                                                  gaagaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGa                                                              >  1:1572381/1‑148 (MQ=255)
                                                                                      aCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAatat                                                          >  2:3182719/1‑148 (MQ=255)
                                                                                       ccACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATc                                                         <  1:3036406/148‑1 (MQ=255)
                                                                                       ccACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATc                                                         <  2:3444460/148‑1 (MQ=255)
                                                                                           ggCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGtt                                                     <  2:462055/148‑1 (MQ=255)
                                                                                           ggCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGtt                                                     <  2:3338269/148‑1 (MQ=255)
                                                                                           ggCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGtt                                                     <  2:1096605/148‑1 (MQ=255)
                                                                                            gCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGttt                                                    >  1:945471/1‑148 (MQ=255)
                                                                                             cGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGtttt                                                   <  1:713879/148‑1 (MQ=255)
                                                                                              gTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTg                                                  <  1:3379951/148‑1 (MQ=255)
                                                                                                   cAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATg                                             >  1:1641547/1‑148 (MQ=255)
                                                                                                   cAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATg                                             <  2:1170487/148‑1 (MQ=255)
                                                                                                      aaGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGt                                          >  1:176637/1‑148 (MQ=255)
                                                                                                          tGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAAc                                      >  1:2922008/1‑148 (MQ=255)
                                                                                                          tGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAAc                                      <  2:2266269/148‑1 (MQ=255)
                                                                                                            aaTTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTg                                    <  2:300738/148‑1 (MQ=255)
                                                                                                             atgggCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATGATCCAGCACAAAGCCCAACCAGTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTgg                                   <  2:2573661/145‑1 (MQ=255)
                                                                                                              ttGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATTGGTTAACTGgc                                  >  2:3550371/1‑148 (MQ=255)
                                                                                                              ttGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGgc                                  >  2:3546910/1‑148 (MQ=255)
                                                                                                                ggCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGgcgc                                >  1:2154468/1‑148 (MQ=255)
                                                                                                                  cGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCgg                              <  2:1410582/148‑1 (MQ=255)
                                                                                                                    tggtgTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCt                            >  1:2362912/1‑148 (MQ=255)
                                                                                                                    tggtgTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCt                            <  1:2265081/148‑1 (MQ=255)
                                                                                                                    tggtgTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCt                            >  2:1905714/1‑148 (MQ=255)
                                                                                                                    tggtgTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCt                            >  1:206282/1‑148 (MQ=255)
                                                                                                                          tCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTcgccgc                      <  1:216863/148‑1 (MQ=255)
                                                                                                                           cGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCgccgcc                     <  2:3284359/148‑1 (MQ=255)
                                                                                                                              ttAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtg                  <  1:138688/148‑1 (MQ=255)
                                                                                                                                  aTACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGGTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGc              >  2:2827798/1‑148 (MQ=255)
                                                                                                                                     cTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCgag           >  1:2001812/1‑148 (MQ=255)
                                                                                                                                          ccTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAg      <  1:125953/148‑1 (MQ=255)
                                                                                                                                          ccTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAg      >  2:125953/1‑148 (MQ=255)
                                                                                                                                           cTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGc     <  2:3063561/148‑1 (MQ=255)
                                                                                                                                            tCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGcc    <  1:2528186/148‑1 (MQ=255)
                                                                                                                                            tCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGcc    <  1:2770477/148‑1 (MQ=255)
                                                                                                                                            tCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGcc    >  1:3138710/1‑148 (MQ=255)
                                                                                                                                              gccgcTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCcac  <  1:121906/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCAC  >  CP009273/3861409‑3861698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGT  >  CP009273/3861404‑3861701
                                                                                                                                                     |                                                                                                                                                    
GAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCA                                                                                                                                                      >  SRR19973066.2838350/1‑150 (MQ=60)
   TGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCT                                                                                                                                                   >  SRR19973066.2151837/1‑150 (MQ=60)
   TGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCT                                                                                                                                                   <  SRR19973066.2240352/150‑1 (MQ=60)
   TGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCT                                                                                                                                                   <  SRR19973066.2300562/150‑1 (MQ=60)
     CCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTT                                                                                                                                                 <  SRR19973066.2004632/150‑1 (MQ=60)
      CTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTG                                                                                                                                                >  SRR19973066.1149479/1‑150 (MQ=60)
      CTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTG                                                                                                                                                >  SRR19973066.1149644/1‑150 (MQ=60)
      CTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTG                                                                                                                                                >  SRR19973066.2395839/1‑150 (MQ=60)
      CTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTG                                                                                                                                                >  SRR19973066.1284807/1‑150 (MQ=60)
           AACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCAT                                                                                                                                           >  SRR19973066.2088449/1‑150 (MQ=60)
           AACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCAT                                                                                                                                           <  SRR19973066.2091560/150‑1 (MQ=60)
           AACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCAT                                                                                                                                           <  SRR19973066.1706658/150‑1 (MQ=60)
           AACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCAT                                                                                                                                           >  SRR19973066.250303/1‑150 (MQ=60)
             CTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGG                                                                                                                                         <  SRR19973066.2743714/150‑1 (MQ=60)
              TGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGG                                                                                                                                        <  SRR19973066.1186643/150‑1 (MQ=60)
              TGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGAGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGG                                                                                                                                        >  SRR19973066.3588121/1‑150 (MQ=60)
                ATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATTGGCA                                                                                                                                      >  SRR19973066.2409769/1‑150 (MQ=60)
                ATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCA                                                                                                                                      >  SRR19973066.2681449/1‑150 (MQ=60)
                   TAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAA                                                                                                                                   >  SRR19973066.496370/1‑150 (MQ=60)
                    AGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAG                                                                                                                                  <  SRR19973066.2750893/150‑1 (MQ=60)
                    AGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAG                                                                                                                                  >  SRR19973066.1182307/1‑150 (MQ=60)
                    AGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAG                                                                                                                                  >  SRR19973066.1182416/1‑150 (MQ=60)
                     GCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGC                                                                                                                                 >  SRR19973066.1635480/1‑150 (MQ=60)
                     GCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGC                                                                                                                                 >  SRR19973066.2216271/1‑150 (MQ=60)
                      CGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCA                                                                                                                                <  SRR19973066.1850316/150‑1 (MQ=60)
                      CGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCA                                                                                                                                <  SRR19973066.2542395/150‑1 (MQ=60)
                       GATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAA                                                                                                                               <  SRR19973066.849501/150‑1 (MQ=60)
                       GATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAA                                                                                                                               <  SRR19973066.849272/150‑1 (MQ=60)
                       GATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAA                                                                                                                               <  SRR19973066.203226/150‑1 (MQ=60)
                        ATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGTCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAT                                                                                                                              >  SRR19973066.3084045/1‑150 (MQ=60)
                        ATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGTCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAT                                                                                                                              >  SRR19973066.3084050/1‑150 (MQ=60)
                         TCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATT                                                                                                                             <  SRR19973066.594233/150‑1 (MQ=60)
                             TTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGAT                                                                                                                         <  SRR19973066.1139818/150‑1 (MQ=60)
                               TCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTA                                                                                                                       <  SRR19973066.864990/150‑1 (MQ=60)
                                CGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTAT                                                                                                                      <  SRR19973066.2495501/150‑1 (MQ=60)
                                   TGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCA                                                                                                                   >  SRR19973066.1592018/1‑150 (MQ=60)
                                   TGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCA                                                                                                                   >  SRR19973066.1591983/1‑150 (MQ=60)
                                      TCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCA                                                                                                                >  SRR19973066.3467502/1‑150 (MQ=60)
                                        CACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCACTTCGATTATCCAGCACA                                                                                                              >  SRR19973066.307583/1‑150 (MQ=60)
                                             CTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCC                                                                                                         >  SRR19973066.239064/1‑150 (MQ=60)
                                              TGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCC                                                                                                        >  SRR19973066.3305977/1‑150 (MQ=60)
                                               GGCCGCCTTCCATCCACGCCAACCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCA                                                                                                       <  SRR19973066.2380189/150‑1 (MQ=60)
                                               GGCCGCCTTCCATCCACGCCAACCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCA                                                                                                       <  SRR19973066.577586/150‑1 (MQ=60)
                                                GCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAA                                                                                                      >  SRR19973066.86458/1‑150 (MQ=60)
                                                GCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAA                                                                                                      >  SRR19973066.3426178/1‑150 (MQ=60)
                                                   GCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCA                                                                                                   >  SRR19973066.3360489/1‑150 (MQ=60)
                                                         CATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCACTTCGATTATCCAGCACAAAGCCCAACCATTCTCC                                                                                             >  SRR19973066.3351539/1‑150 (MQ=60)
                                                         CATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCC                                                                                             >  SRR19973066.922958/1‑150 (MQ=60)
                                                            CCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGA                                                                                          >  SRR19973066.3173383/1‑150 (MQ=60)
                                                                 CCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCTCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGAGCATGGGCACACGCACTTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGAT                                                                                     >  SRR19973066.2747217/1‑150 (MQ=60)
                                                                 CCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGAT                                                                                     <  SRR19973066.35744/150‑1 (MQ=60)
                                                                 CCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGAT                                                                                     <  SRR19973066.1282546/150‑1 (MQ=60)
                                                                 CCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGAT                                                                                     <  SRR19973066.1282480/150‑1 (MQ=60)
                                                                 CCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGAT                                                                                     <  SRR19973066.1923828/150‑1 (MQ=60)
                                                                 CCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGAT                                                                                     <  SRR19973066.2881658/150‑1 (MQ=60)
                                                                 CCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTGTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGAT                                                                                     <  SRR19973066.35746/150‑1 (MQ=60)
                                                                  CAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATG                                                                                    >  SRR19973066.980121/1‑150 (MQ=60)
                                                                   AGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGC                                                                                   <  SRR19973066.2165602/150‑1 (MQ=60)
                                                                    GCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAATGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTAGCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCC                                                                                  <  SRR19973066.2663022/150‑1 (MQ=60)
                                                                         TGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTA                                                                             <  SRR19973066.2216430/150‑1 (MQ=60)
                                                                         TGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTA                                                                             <  SRR19973066.3087700/150‑1 (MQ=60)
                                                                         TGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTA                                                                             <  SRR19973066.3087677/150‑1 (MQ=60)
                                                                          GACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAA                                                                            >  SRR19973066.2028012/1‑150 (MQ=60)
                                                                            CCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAAT                                                                          >  SRR19973066.204683/1‑150 (MQ=60)
                                                                             CATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATG                                                                         >  SRR19973066.1271294/1‑150 (MQ=60)
                                                                             CATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATG                                                                         >  SRR19973066.1271296/1‑150 (MQ=60)
                                                                             CATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATG                                                                         >  SRR19973066.489266/1‑150 (MQ=60)
                                                                              ATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGC                                                                        >  SRR19973066.890322/1‑150 (MQ=60)
                                                                               TCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCA                                                                       >  SRR19973066.1981478/1‑150 (MQ=60)
                                                                                CCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAG                                                                      <  SRR19973066.97381/150‑1 (MQ=60)
                                                                                   GCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACT                                                                   <  SRR19973066.1448503/150‑1 (MQ=60)
                                                                                   GCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACT                                                                   <  SRR19973066.1448486/150‑1 (MQ=60)
                                                                                     GAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGA                                                                 >  SRR19973066.1583718/1‑150 (MQ=60)
                                                                                         AGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATAT                                                             >  SRR19973066.2796285/1‑150 (MQ=60)
                                                                                           ACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCC                                                           <  SRR19973066.1617273/150‑1 (MQ=60)
                                                                                            CCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCG                                                          <  SRR19973066.2994346/150‑1 (MQ=60)
                                                                                            CCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCG                                                          <  SRR19973066.3056698/150‑1 (MQ=60)
                                                                                             CACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGACTATCCGT                                                         >  SRR19973066.1580885/1‑150 (MQ=60)
                                                                                               CGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTT                                                       >  SRR19973066.952372/1‑150 (MQ=60)
                                                                                                GGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTT                                                      <  SRR19973066.1794032/150‑1 (MQ=60)
                                                                                                  CGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGT                                                    <  SRR19973066.719168/150‑1 (MQ=60)
                                                                                                   GTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTT                                                   <  SRR19973066.3402497/150‑1 (MQ=60)
                                                                                                      CGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATG                                                >  SRR19973066.1653487/1‑150 (MQ=60)
                                                                                                      CGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATG                                                >  SRR19973066.1653467/1‑150 (MQ=60)
                                                                                                         AGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGT                                             >  SRR19973066.178195/1‑150 (MQ=60)
                                                                                                         AGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGT                                             >  SRR19973066.1992319/1‑150 (MQ=60)
                                                                                                           AAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTA                                           <  SRR19973066.2198183/150‑1 (MQ=60)
                                                                                                             GGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAAC                                         >  SRR19973066.2941486/1‑150 (MQ=60)
                                                                                                                   TTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGC                                   >  SRR19973066.2169428/1‑150 (MQ=60)
                                                                                                                       CGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCT                               >  SRR19973066.2378922/1‑150 (MQ=60)
                                                                                                                       CGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCT                               >  SRR19973066.208015/1‑150 (MQ=60)
                                                                                                                         TGGTGTTCGGTTAAATACTCACCCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAACGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCG                             <  SRR19973066.3167972/150‑1 (MQ=60)
                                                                                                                         TGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCG                             <  SRR19973066.2280668/150‑1 (MQ=60)
                                                                                                                          GGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGC                            <  SRR19973066.2316092/150‑1 (MQ=60)
                                                                                                                             GTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGC                         >  SRR19973066.3376291/1‑150 (MQ=60)
                                                                                                                               TCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCC                       <  SRR19973066.218643/150‑1 (MQ=60)
                                                                                                                               TCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCC                       <  SRR19973066.218638/150‑1 (MQ=60)
                                                                                                                                   TTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTG                   <  SRR19973066.140024/150‑1 (MQ=60)
                                                                                                                                   TTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTG                   <  SRR19973066.139973/150‑1 (MQ=60)
                                                                                                                                   TTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTG                   <  SRR19973066.1954565/150‑1 (MQ=60)
                                                                                                                                    TAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGG                  >  SRR19973066.266875/1‑150 (MQ=60)
                                                                                                                                      AATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCG                <  SRR19973066.2573091/150‑1 (MQ=60)
                                                                                                                                      AATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCG                <  SRR19973066.773694/150‑1 (MQ=60)
                                                                                                                                        TACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAG              >  SRR19973066.2015099/1‑150 (MQ=60)
                                                                                                                                        TACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAG              >  SRR19973066.2015873/1‑150 (MQ=60)
                                                                                                                                               CCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCC       <  SRR19973066.127202/150‑1 (MQ=60)
                                                                                                                                               CCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCC       >  SRR19973066.3159653/1‑150 (MQ=60)
                                                                                                                                               CCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCC       >  SRR19973066.2388480/1‑150 (MQ=60)
                                                                                                                                               CCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCC       <  SRR19973066.127113/150‑1 (MQ=60)
                                                                                                                                                 TCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCAC     <  SRR19973066.744946/150‑1 (MQ=60)
                                                                                                                                                 TCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCAC     <  SRR19973066.2789132/150‑1 (MQ=60)
                                                                                                                                                 TCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCAC     >  SRR19973066.3077916/1‑150 (MQ=60)
                                                                                                                                                 TCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCAC     <  SRR19973066.2545377/150‑1 (MQ=60)
                                                                                                                                                  CGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACA    >  SRR19973066.2633506/1‑150 (MQ=60)
                                                                                                                                                   GCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACAG   <  SRR19973066.123137/150‑1 (MQ=60)
                                                                                                                                                    CCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGT  >  SRR19973066.949716/1‑150 (MQ=60)
                                                                                                                                                    CCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGT  >  SRR19973066.3575828/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGT  >  CP009273/3861404‑3861701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑