Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,190,061 T→A 73.7% intergenic (+39/‑148) yjaG → / → hupA DUF416 domain protein/HU, DNA‑binding transcriptional regulator, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,190,0610TA73.7% 383.3 / 162.5 209intergenic (+39/‑148)yjaG/hupADUF416 domain protein/HU, DNA‑binding transcriptional regulator, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (23/32);  new base A (70/84);  total (93/116)
Fisher's exact test for biased strand distribution p-value = 7.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 209 total aligned reads displayed.

GACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAC  >  CP009273/4189916‑4190206
                                                                                                                                                 |                                                                                                                                                 
gACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTgg                                                                                                                                                 >  2:3000200/1‑148 (MQ=255)
 tcTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATATAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAAGATTGGTATTATTTTTCAGCAATAAGACCAGAAATCGTGATTTATCGCCTGATTTTTGGTACCAGGa                                                                                                                                                <  2:376816/147‑1 (MQ=255)
 aCTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGa                                                                                                                                                <  2:3173916/148‑1 (MQ=255)
  cTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAg                                                                                                                                               <  1:2783548/148‑1 (MQ=255)
  cTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAg                                                                                                                                               >  2:3259400/1‑148 (MQ=255)
  cTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAg                                                                                                                                               >  1:852950/1‑148 (MQ=255)
      tAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCtt                                                                                                                                           <  1:2530338/148‑1 (MQ=255)
       aGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTc                                                                                                                                          >  2:1036608/1‑148 (MQ=255)
        gCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTcc                                                                                                                                         <  1:1328971/148‑1 (MQ=255)
           gAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCtt                                                                                                                                      >  2:1234265/1‑148 (MQ=255)
              tGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCg                                                                                                                                   >  1:2658840/1‑148 (MQ=255)
              tGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCg                                                                                                                                   >  2:2539422/1‑148 (MQ=255)
              tGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCg                                                                                                                                   >  2:1133347/1‑148 (MQ=255)
                  aagaaCGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGcccc                                                                                                                               <  1:1434822/148‑1 (MQ=255)
                  aagaaCGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGcccc                                                                                                                               <  1:1433141/148‑1 (MQ=255)
                    gaaCGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGcccccc                                                                                                                             >  2:1504923/1‑148 (MQ=255)
                       cgcgATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTc                                                                                                                          <  1:1470278/148‑1 (MQ=255)
                         cgATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTg                                                                                                                        >  2:1463348/1‑148 (MQ=255)
                          gATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTgg                                                                                                                       <  1:3362399/148‑1 (MQ=255)
                            tatCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTc                                                                                                                     >  2:2790616/1‑148 (MQ=255)
                                  gCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACAtt                                                                                                               <  2:1399489/148‑1 (MQ=255)
                                  gCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACAtt                                                                                                               <  2:873171/148‑1 (MQ=255)
                                   cTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGGCTGGTCTACAttt                                                                                                              >  1:2400005/1‑148 (MQ=255)
                                    tGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTg                                                                                                             <  2:1759210/148‑1 (MQ=255)
                                    tGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTg                                                                                                             <  2:3521131/148‑1 (MQ=255)
                                     gATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTgg                                                                                                            <  2:2381558/148‑1 (MQ=255)
                                      aTAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTggg                                                                                                           <  2:231029/148‑1 (MQ=255)
                                        aaaaGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTggggg                                                                                                         >  2:1619372/1‑148 (MQ=255)
                                         aaaGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTgggggg                                                                                                        >  2:485830/1‑148 (MQ=255)
                                         aaaGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTgggggg                                                                                                        <  2:3113726/148‑1 (MQ=255)
                                           aGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCg                                                                                                      >  2:406507/1‑148 (MQ=255)
                                           aGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCg                                                                                                      >  1:764165/1‑148 (MQ=255)
                                              ccTTCGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaaa                                                                                                   <  2:3144712/148‑1 (MQ=255)
                                                  aGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaaaaaaa                                                                                               <  1:3046899/148‑1 (MQ=255)
                                                   gggCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAg                                                                                              >  1:1679495/1‑148 (MQ=255)
                                                    ggCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGt                                                                                             <  1:3150140/148‑1 (MQ=255)
                                                      cAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg                                                                                           <  1:2506295/148‑1 (MQ=255)
                                                       aGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGc                                                                                          <  1:1406990/148‑1 (MQ=255)
                                                           cTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATc                                                                                      <  1:1341386/148‑1 (MQ=255)
                                                               gTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTg                                                                                  <  2:1962679/148‑1 (MQ=255)
                                                                    gCGGGTGAGAGCAATATTGGTATAATTTTTCAGAAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCgtgt                                                                             <  1:3255900/148‑1 (MQ=255)
                                                                     cGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTa                                                                            <  2:3364421/148‑1 (MQ=255)
                                                                         tGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCa                                                                        >  2:2586312/1‑148 (MQ=255)
                                                                          gagaGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAg                                                                       >  2:764358/1‑148 (MQ=255)
                                                                            gagCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGga                                                                     >  1:1122463/1‑148 (MQ=255)
                                                                                aaTATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGt                                                                 <  1:3291690/148‑1 (MQ=255)
                                                                                 atatTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTg                                                                <  1:2310577/148‑1 (MQ=255)
                                                                                  tatTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGc                                                               >  1:3208799/1‑148 (MQ=255)
                                                                                  tatTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGc                                                               >  1:2438094/1‑148 (MQ=255)
                                                                                  tatTGGTATAATTTTTCAGAAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGc                                                               >  2:1541555/1‑148 (MQ=255)
                                                                                    ttGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTa                                                             >  1:595666/1‑148 (MQ=255)
                                                                                     tGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTAt                                                            >  1:356811/1‑148 (MQ=255)
                                                                                        tataATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCt                                                         >  1:1813307/1‑148 (MQ=255)
                                                                                        tataATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCt                                                         >  1:3159678/1‑148 (MQ=255)
                                                                                         ataATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGCTTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCGGGGCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTAATCTg                                                        >  1:1878854/1‑148 (MQ=255)
                                                                                            aTTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCa                                                     <  2:1799759/148‑1 (MQ=255)
                                                                                             tttttCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCAt                                                    <  2:542704/148‑1 (MQ=255)
                                                                                             tttttCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCAt                                                    >  1:3460488/1‑148 (MQ=255)
                                                                                             tttttCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCAt                                                    >  1:2937960/1‑148 (MQ=255)
                                                                                              ttttCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCAtt                                                   >  1:3090760/1‑148 (MQ=255)
                                                                                               tttCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCAttt                                                  <  2:1692318/148‑1 (MQ=255)
                                                                                                ttCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTc                                                 <  1:2649998/148‑1 (MQ=255)
                                                                                                   aGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGt                                              <  2:2147902/148‑1 (MQ=255)
                                                                                                       aTAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTCTTCTGGCATTTCCGTCGCa                                          >  2:2069610/1‑148 (MQ=255)
                                                                                                        tAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCAc                                         >  1:1635493/1‑148 (MQ=255)
                                                                                                        tAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCAc                                         <  2:788561/148‑1 (MQ=255)
                                                                                                        tAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCAc                                         <  1:255574/148‑1 (MQ=255)
                                                                                                         aaGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACt                                        <  1:1959091/148‑1 (MQ=255)
                                                                                                         aaGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACt                                        <  1:3448919/148‑1 (MQ=255)
                                                                                                         aaGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACt                                        >  2:2507968/1‑148 (MQ=255)
                                                                                                          aGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTc                                       <  2:2412608/148‑1 (MQ=255)
                                                                                                           gACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCg                                      >  1:1676490/1‑148 (MQ=255)
                                                                                                            aCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGa                                     >  2:2818444/1‑148 (MQ=255)
                                                                                                             ccAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCCTTTCCGTCGCACTCGAt                                    >  2:1199782/1‑148 (MQ=255)
                                                                                                             ccAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGAt                                    <  1:22848/148‑1 (MQ=255)
                                                                                                             ccAGAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGAt                                    <  1:2719184/148‑1 (MQ=255)
                                                                                                               aGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGc                                  <  1:2346333/148‑1 (MQ=255)
                                                                                                               aGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGc                                  <  2:1122463/148‑1 (MQ=255)
                                                                                                                gAAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCt                                 >  2:307948/1‑148 (MQ=255)
                                                                                                                 aaaaCGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCGTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCtt                                <  2:2355484/148‑1 (MQ=255)
                                                                                                                     cGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCa                            <  2:1772567/148‑1 (MQ=255)
                                                                                                                     cGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCa                            <  2:706652/148‑1 (MQ=255)
                                                                                                                       tGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAg                          <  1:477564/148‑1 (MQ=255)
                                                                                                                        gATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGc                         >  2:672081/1‑148 (MQ=255)
                                                                                                                        gATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGAGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGc                         >  1:1528734/1‑148 (MQ=255)
                                                                                                                          tttAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGa                       >  1:1863924/1‑148 (MQ=255)
                                                                                                                           ttAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGAt                      >  1:2207483/1‑148 (MQ=255)
                                                                                                                             aaCGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATaa                    >  2:2278555/1‑148 (MQ=255)
                                                                                                                               cGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAac                  >  2:719997/1‑148 (MQ=255)
                                                                                                                               cGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAac                  <  2:236167/148‑1 (MQ=255)
                                                                                                                                 ccTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAacac                >  1:2060464/1‑148 (MQ=255)
                                                                                                                                   tGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACAt              >  2:1921914/1‑148 (MQ=255)
                                                                                                                                   tGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACAGTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGATTAGCAAGCGATAAACACAt              >  1:1040764/1‑148 (MQ=255)
                                                                                                                                     aTTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTg            <  2:798255/148‑1 (MQ=255)
                                                                                                                                           cGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGa      <  1:2880542/148‑1 (MQ=255)
                                                                                                                                            gTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGAt     <  2:2937960/148‑1 (MQ=255)
                                                                                                                                             tACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATa    <  1:1056000/148‑1 (MQ=255)
                                                                                                                                             tACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATa    <  1:609278/148‑1 (MQ=255)
                                                                                                                                              aCCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCgtgt                                                                             >  1:1488722/1‑74 (MQ=255)
                                                                                                                                               ccTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAc  >  1:526001/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAC  >  CP009273/4189916‑4190206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTA  >  CP009273/4189916‑4190209
                                                                                                                                                  |                                                                                                                                                    
GACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAG                                                                                                                                                  <  SRR19973066.932611/150‑1 (MQ=60)
GACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAG                                                                                                                                                  >  SRR19973066.859113/1‑150 (MQ=60)
GACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAG                                                                                                                                                  <  SRR19973066.2272720/150‑1 (MQ=60)
  CTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTC                                                                                                                                                <  SRR19973066.2802290/150‑1 (MQ=60)
      TAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCC                                                                                                                                            <  SRR19973066.2547353/150‑1 (MQ=60)
      TAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCC                                                                                                                                            <  SRR19973066.2547543/150‑1 (MQ=60)
       AGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCC                                                                                                                                           >  SRR19973066.3479538/1‑150 (MQ=60)
       AGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCC                                                                                                                                           >  SRR19973066.3275785/1‑150 (MQ=60)
        GCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCT                                                                                                                                          <  SRR19973066.1338606/150‑1 (MQ=60)
          TGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTT                                                                                                                                        <  SRR19973066.1662126/150‑1 (MQ=60)
          TGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTT                                                                                                                                        >  SRR19973066.1871951/1‑150 (MQ=60)
          TGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTT                                                                                                                                        <  SRR19973066.3440216/150‑1 (MQ=60)
            AGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG                                                                                                                                      >  SRR19973066.2676665/1‑150 (MQ=60)
            AGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG                                                                                                                                      >  SRR19973066.2703592/1‑150 (MQ=60)
               GCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCC                                                                                                                                   >  SRR19973066.2396752/1‑150 (MQ=60)
                  AAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCC                                                                                                                                <  SRR19973066.1443364/150‑1 (MQ=60)
                  AAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCC                                                                                                                                <  SRR19973066.1445002/150‑1 (MQ=60)
                  AAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCC                                                                                                                                <  SRR19973066.1445053/150‑1 (MQ=60)
                       CGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTG                                                                                                                           <  SRR19973066.1480646/150‑1 (MQ=60)
                       CGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTG                                                                                                                           <  SRR19973066.1480698/150‑1 (MQ=60)
                          GATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTC                                                                                                                        <  SRR19973066.3384817/150‑1 (MQ=60)
                            TATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTA                                                                                                                      >  SRR19973066.2372369/1‑150 (MQ=60)
                                GAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGTTTTAACGCCTGTTTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATT                                                                                                                  <  SRR19973066.3521915/150‑1 (MQ=60)
                                 AGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTT                                                                                                                 <  SRR19973066.1339944/150‑1 (MQ=60)
                                 AGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGGCTGGTCTACATTT                                                                                                                 >  SRR19973066.2416200/1‑150 (MQ=60)
                                         AAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCG                                                                                                         >  SRR19973066.1704624/1‑150 (MQ=60)
                                         AAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCG                                                                                                         >  SRR19973066.769830/1‑150 (MQ=60)
                                           AGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAA                                                                                                       <  SRR19973066.605925/150‑1 (MQ=60)
                                                 TAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAG                                                                                                 >  SRR19973066.1691719/1‑150 (MQ=60)
                                                 TAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAG                                                                                                 <  SRR19973066.1473689/150‑1 (MQ=60)
                                                  AGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGT                                                                                                <  SRR19973066.3067264/150‑1 (MQ=60)
                                                    GGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGG                                                                                              <  SRR19973066.3171144/150‑1 (MQ=60)
                                                      CAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCT                                                                                            <  SRR19973066.2523361/150‑1 (MQ=60)
                                                      CAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCT                                                                                            >  SRR19973066.804114/1‑150 (MQ=60)
                                                      CAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCT                                                                                            <  SRR19973066.2080119/150‑1 (MQ=60)
                                                      CAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCT                                                                                            <  SRR19973066.1683468/150‑1 (MQ=60)
                                                      CAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCT                                                                                            <  SRR19973066.3175258/150‑1 (MQ=60)
                                                       AGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTA                                                                                           <  SRR19973066.1417105/150‑1 (MQ=60)
                                                          CCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAAACGTGATTTAACGCCTGATTTTTCGTACCAGGAGTCTTCCATTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAATTGGATATC                                                                                         >  SRR19973066.1992569/1‑150 (MQ=60)
                                                           CTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGG                                                                                       <  SRR19973066.1351123/150‑1 (MQ=60)
                                                               GTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCG                                                                                   <  SRR19973066.2656469/150‑1 (MQ=60)
                                                                    GCGGGTGAGAGCAATATTGGTATAATTTTTCAGAAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTAT                                                                              <  SRR19973066.3277328/150‑1 (MQ=60)
                                                                      GGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGC                                                                            <  SRR19973066.2446517/150‑1 (MQ=60)
                                                                        GTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAG                                                                          <  SRR19973066.54484/150‑1 (MQ=60)
                                                                        GTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAG                                                                          <  SRR19973066.2517317/150‑1 (MQ=60)
                                                                          GAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGA                                                                        >  SRR19973066.1130551/1‑150 (MQ=60)
                                                                           AGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAG                                                                       <  SRR19973066.3290636/150‑1 (MQ=60)
                                                                             AGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAG                                                                     <  SRR19973066.2671186/150‑1 (MQ=60)
                                                                                AATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGC                                                                  >  SRR19973066.3230066/1‑150 (MQ=60)
                                                                                AATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGC                                                                  <  SRR19973066.3313409/150‑1 (MQ=60)
                                                                                AATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGC                                                                  >  SRR19973066.3230074/1‑150 (MQ=60)
                                                                                AATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGC                                                                  >  SRR19973066.2454701/1‑150 (MQ=60)
                                                                                 ATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCT                                                                 <  SRR19973066.2326391/150‑1 (MQ=60)
                                                                                  TATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTA                                                                >  SRR19973066.599787/1‑150 (MQ=60)
                                                                                  TATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTA                                                                <  SRR19973066.1117348/150‑1 (MQ=60)
                                                                                   ATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTAT                                                               >  SRR19973066.359593/1‑150 (MQ=60)
                                                                                    TTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATT                                                              <  SRR19973066.2772663/150‑1 (MQ=60)
                                                                                      GGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCT                                                            >  SRR19973066.1826212/1‑150 (MQ=60)
                                                                                      GGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCT                                                            >  SRR19973066.3180733/1‑150 (MQ=60)
                                                                                      GGTAGAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCT                                                            <  SRR19973066.3151836/150‑1 (MQ=60)
                                                                                       GTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGCTTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCGGGGCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTAATCTG                                                           >  SRR19973066.1892269/1‑150 (MQ=60)
                                                                                           AATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCAT                                                       >  SRR19973066.3483627/1‑150 (MQ=60)
                                                                                           AATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCAT                                                       >  SRR19973066.2957497/1‑150 (MQ=60)
                                                                                           AATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCAT                                                       >  SRR19973066.2957385/1‑150 (MQ=60)
                                                                                           AATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGCCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCAT                                                       >  SRR19973066.1425238/1‑150 (MQ=60)
                                                                                            ATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATT                                                      >  SRR19973066.3111407/1‑150 (MQ=60)
                                                                                            ATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATT                                                      <  SRR19973066.2321604/150‑1 (MQ=60)
                                                                                             TTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTT                                                     <  SRR19973066.1543421/150‑1 (MQ=60)
                                                                                               TTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCC                                                   <  SRR19973066.256648/150‑1 (MQ=60)
                                                                                               TTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCC                                                   <  SRR19973066.715804/150‑1 (MQ=60)
                                                                                                TTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCG                                                  <  SRR19973066.2667787/150‑1 (MQ=60)
                                                                                                   AGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCG                                               >  SRR19973066.398859/1‑150 (MQ=60)
                                                                                                      AATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCAC                                            >  SRR19973066.1647383/1‑150 (MQ=60)
                                                                                                        TAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTC                                          <  SRR19973066.257511/150‑1 (MQ=60)
                                                                                                         AAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTNG                                         <  SRR19973066.461759/150‑1 (MQ=60)
                                                                                                         AAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCG                                         <  SRR19973066.1972946/150‑1 (MQ=60)
                                                                                                         AAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGTCGAAAAAAAGTGGCTATCTGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCG                                         >  SRR19973066.1688702/1‑150 (MQ=60)
                                                                                                         AAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCG                                         >  SRR19973066.1688700/1‑150 (MQ=60)
                                                                                                         AAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCG                                         <  SRR19973066.3471979/150‑1 (MQ=60)
                                                                                                             CCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGC                                     <  SRR19973066.2737469/150‑1 (MQ=60)
                                                                                                             CCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGC                                     <  SRR19973066.23182/150‑1 (MQ=60)
                                                                                                               AG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTT                                   <  SRR19973066.2362293/150‑1 (MQ=60)
                                                                                                                    AACGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCA                               <  SRR19973066.1515403/150‑1 (MQ=60)
                                                                                                                      CGTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAG                             <  SRR19973066.3380168/150‑1 (MQ=60)
                                                                                                                       GTGATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGAGAAAAAAAGTGGCTATCGGTGCGTGTATGAAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGC                            >  SRR19973066.1539582/1‑150 (MQ=60)
                                                                                                                        TGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCG                           <  SRR19973066.481166/150‑1 (MQ=60)
                                                                                                                         GATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGA                          >  SRR19973066.1877631/1‑150 (MQ=60)
                                                                                                                         GATTTAACGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGA                          >  SRR19973066.1877241/1‑150 (MQ=60)
                                                                                                                          ATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGAT                         >  SRR19973066.2222777/1‑150 (MQ=60)
                                                                                                                                CGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACAC                   >  SRR19973066.2074750/1‑150 (MQ=60)
                                                                                                                                CGCCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACAC                   >  SRR19973066.139877/1‑150 (MQ=60)
                                                                                                                                  CCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACAT                 >  SRR19973066.1048521/1‑150 (MQ=60)
                                                                                                                                  CCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACAT                 >  SRR19973066.3022700/1‑150 (MQ=60)
                                                                                                                                  CCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACAT                 <  SRR19973066.1141472/150‑1 (MQ=60)
                                                                                                                                  CCTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACAGTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGATTAGCAAGCGATAAACACAT                 >  SRR19973066.1048421/1‑150 (MQ=60)
                                                                                                                                   CTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATT                <  SRR19973066.3020123/150‑1 (MQ=60)
                                                                                                                                   CTGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATT                >  SRR19973066.3091233/1‑150 (MQ=60)
                                                                                                                                    TGATTTGTCGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTG               <  SRR19973066.1926491/150‑1 (MQ=60)
                                                                                                                                            CGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATA       <  SRR19973066.2899823/150‑1 (MQ=60)
                                                                                                                                            CGTACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATA       <  SRR19973066.2899835/150‑1 (MQ=60)
                                                                                                                                             GTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATagatcggaagagcacacgtctgaactccagtcactaccaatgcgatctcgttttccgtgttgtgggtgtaaa      >  SRR19973066.1499153/1‑78 (MQ=60)
                                                                                                                                              TACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAC     >  SRR19973066.529735/1‑150 (MQ=60)
                                                                                                                                              TACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAC     >  SRR19973066.529855/1‑150 (MQ=60)
                                                                                                                                              TACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAC     <  SRR19973066.1063761/150‑1 (MQ=60)
                                                                                                                                              TACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAC     >  SRR19973066.2216347/1‑150 (MQ=60)
                                                                                                                                              TACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAC     <  SRR19973066.613515/150‑1 (MQ=60)
                                                                                                                                              TACCAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTAACGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAC     <  SRR19973066.2337063/150‑1 (MQ=60)
                                                                                                                                                 CAGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTA  <  SRR19973066.781452/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
GACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAG‑AAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTA  >  CP009273/4189916‑4190209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑