Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 132,639 T→A 26.2% T105S (ACG→TCG)  speE ← spermidine synthase (putrescine aminopropyltransferase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273132,6390TA26.2% 439.9 / 203.7 244T105S (ACG→TCG) speEspermidine synthase (putrescine aminopropyltransferase)
Reads supporting (aligned to +/- strand):  ref base T (88/91);  new base A (34/30);  total (123/121)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.28e-01

Only 100 of 246 total aligned reads displayed.

GACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCGCTCGGTGG  >  CP009273/132490‑132788
                                                                                                                                                     |                                                                                                                                                     
   aTCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGAt                                                                                                                                                    <  1:3026397/150‑1 (MQ=255)
   aTCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGAt                                                                                                                                                    >  2:3615847/1‑150 (MQ=255)
    tCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGAtt                                                                                                                                                   <  2:611273/150‑1 (MQ=255)
    tCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGAtt                                                                                                                                                   <  2:723768/150‑1 (MQ=255)
     cAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTg                                                                                                                                                  <  2:2155891/150‑1 (MQ=255)
      aaaGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGa                                                                                                                                                 >  2:4089033/1‑150 (MQ=255)
       aaGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGAc                                                                                                                                                >  2:1435863/1‑150 (MQ=255)
          gTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCa                                                                                                                                             <  2:2635182/150‑1 (MQ=255)
            ctggctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAAc                                                                                                                                           >  2:3178974/1‑150 (MQ=255)
             tggctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATTGTTTGGTAGTTACTGACCGCAGAAAGATAAGACAACCGCATCGATTTACACCATCGTGATTTAATCAACg                                                                                                                                          >  1:4165218/1‑150 (MQ=255)
             tggctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACg                                                                                                                                          >  2:794889/1‑150 (MQ=255)
             tggctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACg                                                                                                                                          >  2:132785/1‑150 (MQ=255)
             tggctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGGAGATACAGACGGCACAACGATACGACACCCCCATCGATTTCCACCAACGTGATTGACTCCAcc                                                                                                                                          >  1:3050555/1‑149 (MQ=255)
                ctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGttt                                                                                                                                       >  2:893312/1‑150 (MQ=255)
                ctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGttt                                                                                                                                       >  1:2155158/1‑150 (MQ=255)
                ctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGttt                                                                                                                                       <  1:2339941/150‑1 (MQ=255)
                ctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATAGATTTACACCAACGAGATTTACTCAAAGttt                                                                                                                                       >  1:3061585/1‑150 (MQ=255)
                ctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTGATGGTTGGGTAGATACATACGGCAGAAACATACGACACCCGCATCGATTTCCACCAACGTGAATGACGCAACGttt                                                                                                                                       >  1:3793844/1‑150 (MQ=255)
                  ggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGttttt                                                                                                                                     >  1:1579571/1‑150 (MQ=255)
                   gtcggATTCACGAACTTGACGCCATCGTCCATCACCAGCTGACAGCGCGGAGCGTCGGAGCTACCGGCGTTAGGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCACCGTGATGGACTCAACGTTTTTa                                                                                                                                    <  2:449933/146‑1 (MQ=255)
                     ttGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATAAGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATg                                                                                                                                  >  1:3527227/1‑150 (MQ=255)
                       gATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTc                                                                                                                                <  1:193362/150‑1 (MQ=255)
                        aTTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCg                                                                                                                               >  2:1706184/1‑150 (MQ=255)
                        aTTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACAGACGGCAGAACGATAAGACACCCCCCACGATTTACACCCAAGTGATTGACAAAAACTTTTTAtttag                                                                                                                               >  1:4120392/1‑146 (MQ=255)
                           aaCGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGt                                                                                                                            <  1:3516425/150‑1 (MQ=255)
                            aCGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGtt                                                                                                                           <  2:15340/150‑1 (MQ=255)
                             cGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTa                                                                                                                          >  2:2240188/1‑150 (MQ=255)
                             cGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTACACCAACGTGCTTGACTCAACGTTTTTATGTCGGGTTa                                                                                                                          >  1:3411886/1‑150 (MQ=255)
                               aaaTTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACt                                                                                                                        <  2:1920683/150‑1 (MQ=255)
                                 aTTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTc                                                                                                                      >  2:925678/1‑150 (MQ=255)
                                  ttGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCa                                                                                                                     <  1:4323488/150‑1 (MQ=255)
                                    gACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACg                                                                                                                   <  1:1003619/150‑1 (MQ=255)
                                        ccATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACgcagc                                                                                                               <  1:1858550/150‑1 (MQ=255)
                                        ccATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACgcagc                                                                                                               <  2:3921082/150‑1 (MQ=255)
                                        ccATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACgcagc                                                                                                               <  1:4048317/150‑1 (MQ=255)
                                          aTCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCAt                                                                                                             <  1:203234/150‑1 (MQ=255)
                                            cgtcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTGTTGGTTTGGTAGATAATGAAGGCAGAACGATACGACACCCCCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCAAgg                                                                                                           >  1:1177334/1‑150 (MQ=255)
                                              tcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCa                                                                                                         <  1:503742/150‑1 (MQ=255)
                                                    aCCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTcg                                                                                                   >  1:3131913/1‑150 (MQ=255)
                                                            aaaGCGCGGATCGTCGTAGCTACCGGCGTTATTGTTGGGTAGATAATGACGGCAGAACGATAAGACACCCGCATCGATTTACACCAACGAGATTGTCTCAACGGTTTTATTTGGGGTTAATTCACGCAGCATTGCACCCTCgccgccccc                                                                                           >  1:3707913/1‑150 (MQ=255)
                                                              aGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGa                                                                                         <  1:667620/150‑1 (MQ=255)
                                                              aGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGa                                                                                         <  1:252494/150‑1 (MQ=255)
                                                               gcgcGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGAt                                                                                        <  2:2876485/150‑1 (MQ=255)
                                                               gcgcGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGAt                                                                                        <  2:3181976/150‑1 (MQ=255)
                                                               gcgcGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGAt                                                                                        <  2:3305727/150‑1 (MQ=255)
                                                                cgcgGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATa                                                                                       <  1:1627554/150‑1 (MQ=255)
                                                                cgcgGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATa                                                                                       <  1:2554659/150‑1 (MQ=255)
                                                                cgcgGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCAACGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATa                                                                                       >  1:1765881/1‑150 (MQ=255)
                                                                  cgGATCGTCGTAGCTACCGGCGTTTTGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACGCAACGTTTTTATGTAGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAAt                                                                                     >  1:3775134/1‑150 (MQ=255)
                                                                  cgGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAAt                                                                                     >  2:1044564/1‑150 (MQ=255)
                                                                   ggATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATATGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATc                                                                                    <  2:2690993/150‑1 (MQ=255)
                                                                              gCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGttt                                                                         >  2:1368241/1‑150 (MQ=255)
                                                                              gCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACAAGttt                                                                         >  2:2783653/1‑150 (MQ=255)
                                                                                tACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTTACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACAAGtttag                                                                       >  2:1842316/1‑148 (MQ=255)
                                                                                    ggCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACTCCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTg                                                                   <  1:682731/150‑1 (MQ=255)
                                                                                    ggCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTg                                                                   >  1:2201507/1‑150 (MQ=255)
                                                                                       gTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGAcc                                                                >  2:367857/1‑150 (MQ=255)
                                                                                       gTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGAcc                                                                >  1:3911568/1‑150 (MQ=255)
                                                                                           tGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATgg                                                            <  1:2014443/150‑1 (MQ=255)
                                                                                              ttGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGcc                                                         >  1:3672679/1‑150 (MQ=255)
                                                                                                gggTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAg                                                       >  1:4264637/1‑150 (MQ=255)
                                                                                                 ggTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGt                                                      <  1:930840/150‑1 (MQ=255)
                                                                                                   tAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAg                                                    >  2:2702749/1‑150 (MQ=255)
                                                                                                   tAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGAGTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAg                                                    >  2:530580/1‑150 (MQ=255)
                                                                                                    aGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGc                                                   >  2:3251767/1‑150 (MQ=255)
                                                                                                         cTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAAc                                              >  2:1739196/1‑150 (MQ=255)
                                                                                                         cTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAAc                                              >  1:3235033/1‑150 (MQ=255)
                                                                                                           gACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACAt                                            <  2:1472371/150‑1 (MQ=255)
                                                                                                           gACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACAt                                            <  1:542803/150‑1 (MQ=255)
                                                                                                            aCGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATg                                           <  1:1468199/150‑1 (MQ=255)
                                                                                                            aCGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATg                                           <  2:2962563/150‑1 (MQ=255)
                                                                                                             cGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATgg                                          <  1:2959249/150‑1 (MQ=255)
                                                                                                               gCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGt                                        <  2:2419181/150‑1 (MQ=255)
                                                                                                                cAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGtc                                       <  2:3038220/150‑1 (MQ=255)
                                                                                                                cAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGtc                                       >  1:2292476/1‑150 (MQ=255)
                                                                                                                  gAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGtcat                                     <  1:302387/150‑1 (MQ=255)
                                                                                                                     cGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCCCATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTcatcat                                  >  1:1121781/1‑150 (MQ=255)
                                                                                                                        tACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCAtctc                               >  1:3328160/1‑150 (MQ=255)
                                                                                                                        tACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCAtctc                               <  1:4055190/150‑1 (MQ=255)
                                                                                                                         aCGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCa                              <  1:2927370/150‑1 (MQ=255)
                                                                                                                         aCGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCa                              <  2:3670509/150‑1 (MQ=255)
                                                                                                                          cGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCAt                             >  2:3462695/1‑150 (MQ=255)
                                                                                                                          cGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGATTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCAGGGGCCAGTAGCGGAACATGGGTCATCATCTCAt                             <  2:1163576/150‑1 (MQ=255)
                                                                                                                          cGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCAt                             >  2:3498269/1‑150 (MQ=255)
                                                                                                                           gACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATg                            <  2:3536167/150‑1 (MQ=255)
                                                                                                                               cccGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATgatag                        <  1:271472/150‑1 (MQ=255)
                                                                                                                               cccGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATgatag                        <  1:3898525/150‑1 (MQ=255)
                                                                                                                                ccGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTGTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATgataga                       >  1:2781646/1‑150 (MQ=255)
                                                                                                                                 cGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATgatagat                      <  2:94634/150‑1 (MQ=255)
                                                                                                                                     tCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAAc                  <  1:1702050/150‑1 (MQ=255)
                                                                                                                                     tCGATGTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaacc                  <  1:3403842/150‑3 (MQ=255)
                                                                                                                                      cGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACt                 >  1:732863/1‑150 (MQ=255)
                                                                                                                                       gATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACtc                >  1:2091115/1‑150 (MQ=255)
                                                                                                                                        aTTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACtcg               >  1:953165/1‑150 (MQ=255)
                                                                                                                                         tttCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACtcgt              <  2:1579571/150‑1 (MQ=255)
                                                                                                                                               ccaTCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGAGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCGCt        <  1:1293753/150‑1 (MQ=255)
                                                                                                                                                 aTCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCGCTCg      <  2:3860378/150‑1 (MQ=255)
                                                                                                                                                   cGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCGCTCggt    <  1:400439/150‑1 (MQ=255)
                                                                                                                                                    gTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCGCTCggtg   <  2:1981933/150‑1 (MQ=255)
                                                                                                                                                     tGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCGCTCggtgg  <  2:2434524/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCGCTCGGTGG  >  CP009273/132490‑132788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A