Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 307,454 G→A 29.0% intergenic (‑407/‑369) ecpR ← / → ykgL putative transcriptional regulator for the ecp operon/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273307,4540GA29.0% 332.8 / 166.7 193intergenic (‑407/‑369)ecpR/ykgLputative transcriptional regulator for the ecp operon/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (81/56);  new base A (29/27);  total (110/83)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 197 total aligned reads displayed.

GTTATTGGCAATATAACGTTCAATTCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCGGTAAGCCCCCAAGAG  >  CP009273/307306‑307603
                                                                                                                                                    |                                                                                                                                                     
    ttGGCAATATAACGTTCAATTCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAg                                                                                                                                                  >  1:2773461/1‑150 (MQ=255)
      ggCAATATAACGTTCAATTCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCt                                                                                                                                                >  1:3982321/1‑150 (MQ=255)
      ggCAATATAACGTTCAATTCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCt                                                                                                                                                <  1:2243198/150‑1 (MQ=255)
      ggCAATATAACGTTCAATTCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCt                                                                                                                                                <  1:2243172/150‑1 (MQ=255)
             taACGTTCAATTCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTg                                                                                                                                         <  2:17959/150‑1 (MQ=255)
               aCGTTCAATTCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGaa                                                                                                                                       <  1:3048577/150‑1 (MQ=255)
                 gTTCAATTCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAga                                                                                                                                     >  2:2347137/1‑150 (MQ=255)
                 gTTCAATTCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAga                                                                                                                                     >  1:4040150/1‑150 (MQ=255)
                  ttCAATTCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAgat                                                                                                                                    <  2:2990598/150‑1 (MQ=255)
                  ttCAATTCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAgat                                                                                                                                    <  2:272757/150‑1 (MQ=255)
                  ttCAATTCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAgat                                                                                                                                    >  1:3840090/1‑150 (MQ=255)
                   tCAATTCTTACTTGCATTTTTTATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAgatg                                                                                                                                   <  2:1901911/150‑1 (MQ=255)
                    cAATTCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAgatga                                                                                                                                  >  2:1368789/1‑150 (MQ=255)
                       ttCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGAttt                                                                                                                               >  1:3585223/1‑150 (MQ=255)
                        tCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTg                                                                                                                              <  2:1276962/150‑1 (MQ=255)
                            aCTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCaa                                                                                                                          >  2:4056145/1‑150 (MQ=255)
                             cTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAAt                                                                                                                         <  2:83190/150‑1 (MQ=255)
                               tGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCa                                                                                                                       <  1:2669626/150‑1 (MQ=255)
                                gCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAg                                                                                                                      >  1:3945977/1‑150 (MQ=255)
                                  aTTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTa                                                                                                                    >  1:2332079/1‑150 (MQ=255)
                                  aTTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTa                                                                                                                    >  2:1640842/1‑150 (MQ=255)
                                    tttATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAg                                                                                                                  <  1:910790/150‑1 (MQ=255)
                                     ttATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGa                                                                                                                 >  1:1103436/1‑150 (MQ=255)
                                     ttATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGa                                                                                                                 >  1:412657/1‑150 (MQ=255)
                                      tataTTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGat                                                                                                                >  2:735472/1‑150 (MQ=255)
                                       atatTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGata                                                                                                               <  2:3862460/150‑1 (MQ=255)
                                       atatTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGata                                                                                                               <  2:1673428/150‑1 (MQ=255)
                                        tatTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGatat                                                                                                              <  2:1573367/150‑1 (MQ=255)
                                         atTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATAtt                                                                                                             <  1:278837/150‑1 (MQ=255)
                                               cAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATTAGCTGTCATTGAAGATGATTTTCCAATCAGTAAGATATTTTAATg                                                                                                       >  2:3261373/1‑150 (MQ=255)
                                               cAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATg                                                                                                       >  1:31326/1‑150 (MQ=255)
                                                   aaCTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGAtt                                                                                                   <  2:607572/150‑1 (MQ=255)
                                                      tttGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAAc                                                                                                >  1:2113575/1‑150 (MQ=255)
                                                        tGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACAt                                                                                              >  1:1449922/1‑150 (MQ=255)
                                                         gCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATg                                                                                             <  1:1269214/150‑1 (MQ=255)
                                                          cccTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGc                                                                                            <  2:1861503/150‑1 (MQ=255)
                                                           ccTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGcc                                                                                           >  1:1626263/1‑150 (MQ=255)
                                                            cTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCg                                                                                          >  1:819314/1‑150 (MQ=255)
                                                              gAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGca                                                                                        >  1:1619893/1‑150 (MQ=255)
                                                                        gAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTATTGGATTAACATGCCGCACAAAATGGtt                                                                              <  2:1313020/150‑1 (MQ=255)
                                                                        gAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAACTGGtt                                                                              <  2:1313184/150‑1 (MQ=255)
                                                                         aaTAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTa                                                                             >  1:1923787/1‑150 (MQ=255)
                                                                         aaTAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTa                                                                             >  1:2189860/1‑150 (MQ=255)
                                                                         aaTAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTa                                                                             <  2:2575527/150‑1 (MQ=255)
                                                                         aaTAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTa                                                                             <  1:616422/150‑1 (MQ=255)
                                                                          aTAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATAATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTAc                                                                            <  2:3480759/150‑1 (MQ=255)
                                                                                ttAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGgtgt                                                                      <  1:4293010/150‑1 (MQ=255)
                                                                                  aaTGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTc                                                                    >  2:108236/1‑150 (MQ=255)
                                                                                       gcTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTcc                                                               <  2:1209193/150‑1 (MQ=255)
                                                                                        cTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCa                                                              <  2:2540861/150‑1 (MQ=255)
                                                                                         tgtgtgATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCaa                                                             >  2:418440/1‑150 (MQ=255)
                                                                                         tgtgtgATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCaa                                                             <  1:1730054/150‑1 (MQ=255)
                                                                                          gtgtgATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAAt                                                            <  1:4346599/150‑1 (MQ=255)
                                                                                             tgATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAg                                                         <  2:1500017/150‑1 (MQ=255)
                                                                                              gATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGt                                                        >  2:3832280/1‑150 (MQ=255)
                                                                                                 ggACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAAt                                                     >  2:2094670/1‑150 (MQ=255)
                                                                                                 ggACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAAt                                                     >  2:2626573/1‑150 (MQ=255)
                                                                                                 ggACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAAt                                                     >  2:2626549/1‑150 (MQ=255)
                                                                                                  gACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATg                                                    >  1:99827/1‑150 (MQ=255)
                                                                                                    cTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGa                                                  >  1:2580185/1‑150 (MQ=255)
                                                                                                    cTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGa                                                  >  1:40629/1‑150 (MQ=255)
                                                                                                      aGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGaaa                                                >  1:2208066/1‑150 (MQ=255)
                                                                                                        tAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTTAAGATGATTTGACAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCAAATAAGTCCTGGACAAt                                              >  2:902214/1‑150 (MQ=255)
                                                                                                         aaaaaaaCAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAAtt                                             >  2:4159660/1‑150 (MQ=255)
                                                                                                            aaaaCAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGaa                                          >  1:3523389/1‑150 (MQ=255)
                                                                                                            aaaaCAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGaa                                          >  2:3363716/1‑150 (MQ=255)
                                                                                                            aaaaCAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGaa                                          >  1:3885276/1‑150 (MQ=255)
                                                                                                            aaaaCAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGaa                                          >  1:604874/1‑150 (MQ=255)
                                                                                                            aaaaCAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATAGaa                                          >  1:1568425/1‑150 (MQ=255)
                                                                                                             aaaCAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAAc                                         >  2:3080152/1‑150 (MQ=255)
                                                                                                             aaaCAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAAc                                         <  1:2043234/150‑1 (MQ=255)
                                                                                                             aaaCAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAAc                                         <  1:4229337/150‑1 (MQ=255)
                                                                                                              aaCAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACg                                        >  2:1243945/1‑150 (MQ=255)
                                                                                                                 aaGTTTTAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGc                                     >  2:4288333/1‑150 (MQ=255)
                                                                                                                  aGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATGTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCg                                    <  2:3063477/150‑1 (MQ=255)
                                                                                                                    ttttAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTa                                  <  1:1640842/150‑1 (MQ=255)
                                                                                                                    ttttAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTa                                  <  2:3427839/150‑1 (MQ=255)
                                                                                                                     tttAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTAt                                 <  2:4183108/150‑1 (MQ=255)
                                                                                                                      ttAAGGGTAAGGGATAGAATATAAAACTATAATGAGCTGTAATTGAAGATTATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATg                                <  1:3700517/150‑1 (MQ=255)
                                                                                                                         agggtaagggATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTAc                             >  2:226053/1‑150 (MQ=255)
                                                                                                                          gggtaagggATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACg                            <  1:124339/150‑1 (MQ=255)
                                                                                                                          gggtaagggATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACg                            >  2:1443251/1‑150 (MQ=255)
                                                                                                                             taagggATAGAATATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCa                         <  2:4215945/150‑1 (MQ=255)
                                                                                                                              aagggATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGTACGGGCGTATGTAGGTCAt                        >  2:2649545/1‑150 (MQ=255)
                                                                                                                              aagggATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCAt                        <  2:2196081/150‑1 (MQ=255)
                                                                                                                               aggaATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATc                       >  1:3732177/1‑150 (MQ=255)
                                                                                                                                    tAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTc                  <  1:735472/150‑1 (MQ=255)
                                                                                                                                    tAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTc                  <  1:860179/150‑1 (MQ=255)
                                                                                                                                     aGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCg                 <  2:2211396/150‑1 (MQ=255)
                                                                                                                                      gAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCgg                <  1:3083954/150‑1 (MQ=255)
                                                                                                                                       aaTATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCGGt               >  1:1184746/1‑150 (MQ=255)
                                                                                                                                       aaTATAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCGGt               >  2:3105858/1‑150 (MQ=255)
                                                                                                                                        atatAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCGGTa              >  1:579145/1‑150 (MQ=255)
                                                                                                                                        atatAAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCGGTa              >  2:1037795/1‑150 (MQ=255)
                                                                                                                                         tataAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCGGTaa             <  2:2168119/150‑1 (MQ=255)
                                                                                                                                           taAAACTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCGGTAAGc           <  2:4040150/150‑1 (MQ=255)
                                                                                                                                             aaaCTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCGGTAAGccc         >  2:2872036/1‑150 (MQ=255)
                                                                                                                                               aCTATAATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCGGTAAGccccc       >  2:1423147/1‑150 (MQ=255)
                                                                                                                                                cTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCGGTAAGCCCCCa      >  1:1795486/1‑150 (MQ=255)
                                                                                                                                                    gatgaGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCGGTAAGCCCCCAagag  <  2:3585223/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTTATTGGCAATATAACGTTCAATTCTTACTTGCATTTTATATTGGTCAGGAACTTTGCCCTGAAGTGTTAGGAATAGTCTTAATGCGCTGTGTGATGGACTAGTAAAAAAACAAGTTTTAAGGGTAAGGGATAGAATATAAAACTATGATGAGCTGTCATTGAAGATGATTTGCCAATCAGTAAGATATTTTAATGGATTAACATGCCGCACAAAATGGTTACAGGTGTTCATTCCAATAAGTAATGGAAAATTGAACGGGCGTATGTACGTCATCTGTTCGGTAAGCCCCCAAGAG  >  CP009273/307306‑307603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A