Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 925,957 A→T 15.4% I154I (ATT→ATA)  cydD ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273925,9570AT15.4% 832.7 / 125.8 290I154I (ATT→ATA) cydDfused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base A (117/128);  new base T (13/32);  total (130/160)
Fisher's exact test for biased strand distribution p-value = 2.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 296 total aligned reads displayed.

CCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAATCCACGCTGGCCCTGCTTGTTGCAGACG  >  CP009273/925810‑926106
                                                                                                                                                   |                                                                                                                                                     
cccACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAAt                                                                                                                                                     >  1:4166824/1‑150 (MQ=255)
  cACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCa                                                                                                                                                   <  1:2904244/150‑1 (MQ=255)
  cACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCa                                                                                                                                                   <  1:458060/150‑1 (MQ=255)
      tAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCaa                                                                                                                                               >  2:1543733/1‑150 (MQ=255)
      tAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCaa                                                                                                                                               <  1:1419550/150‑1 (MQ=255)
      tAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCaa                                                                                                                                               >  2:556212/1‑150 (MQ=255)
       aaGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAAc                                                                                                                                              >  2:3753330/1‑150 (MQ=255)
        aGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACg                                                                                                                                             <  1:26653/150‑1 (MQ=255)
        aGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACg                                                                                                                                             <  2:1864326/150‑1 (MQ=255)
          agAGCAAGATCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAAAAACGGAATAAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACTATCAGCAACGGc                                                                                                                                           >  2:659346/2‑150 (MQ=255)
           gAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCa                                                                                                                                          >  1:1178181/1‑150 (MQ=255)
           gAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCa                                                                                                                                          <  2:279027/150‑1 (MQ=255)
             gCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACTATCAGCAACGGCAcc                                                                                                                                        <  1:1873911/150‑1 (MQ=255)
             gCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCAcc                                                                                                                                        <  1:253003/150‑1 (MQ=255)
              cAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCg                                                                                                                                       <  1:593956/150‑1 (MQ=255)
              cAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCg                                                                                                                                       >  2:2415980/1‑150 (MQ=255)
               aaGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGa                                                                                                                                      >  2:2053674/1‑150 (MQ=255)
                agagCGATAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGac                                                                                                                                     <  1:2660694/150‑1 (MQ=255)
                     gagaAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGc                                                                                                                                >  2:757154/1‑150 (MQ=255)
                     gagaAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGc                                                                                                                                >  1:2386784/1‑150 (MQ=255)
                     gagaAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGc                                                                                                                                >  2:4261099/1‑150 (MQ=255)
                       gaAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCACCCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCa                                                                                                                              <  1:3314243/150‑1 (MQ=255)
                       gaAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCa                                                                                                                              <  2:4163871/150‑1 (MQ=255)
                       gaAAGGTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCa                                                                                                                              <  2:3143101/150‑1 (MQ=255)
                             tACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCa                                                                                                                        <  2:4215592/150‑1 (MQ=255)
                               cGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCAtt                                                                                                                      >  1:259810/1‑150 (MQ=255)
                                  cGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGc                                                                                                                   >  2:4037869/1‑150 (MQ=255)
                                        gCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGa                                                                                                             <  2:903350/150‑1 (MQ=255)
                                        gCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGa                                                                                                             <  2:862335/150‑1 (MQ=255)
                                         cATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCCTTTGCGGCAGAt                                                                                                            >  2:4325526/1‑150 (MQ=255)
                                              gCAGCCCCCAGTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGt                                                                                                       <  1:2708581/150‑1 (MQ=255)
                                                aGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACTATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGc                                                                                                     >  1:2517482/1‑150 (MQ=255)
                                                 gCCCACATTCCAACCAGCGCCATAACCATCGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACTCTCAGCACCGGCACCGACACTGACAGCGCCATTTGCGGCAGATAGCGTGCa                                                                                                    <  2:2154136/150‑1 (MQ=255)
                                                   ccccATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATa                                                                                                  >  1:3499536/1‑150 (MQ=255)
                                                    cccATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAg                                                                                                 >  2:1912802/1‑150 (MQ=255)
                                                     ccATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACTATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGt                                                                                                <  1:555162/150‑1 (MQ=255)
                                                     ccATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGt                                                                                                >  2:326598/1‑150 (MQ=255)
                                                        ttCCAACCAGCGCCATAAACAACGTAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAAt                                                                                             <  1:1896259/150‑1 (MQ=255)
                                                         tCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATc                                                                                            >  1:3050624/1‑150 (MQ=255)
                                                         tCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATc                                                                                            <  1:2824670/150‑1 (MQ=255)
                                                          ccAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCa                                                                                           <  2:1770473/150‑1 (MQ=255)
                                                              ccAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCa                                                                                       >  1:1806948/1‑150 (MQ=255)
                                                                 gcgcCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCatat                                                                                    <  2:1980669/150‑1 (MQ=255)
                                                                  cgcCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATc                                                                                   >  2:3628756/1‑150 (MQ=255)
                                                                     cATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTc                                                                                <  2:3429033/150‑1 (MQ=255)
                                                                       tAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACTATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCaa                                                                              <  1:1065492/150‑1 (MQ=255)
                                                                       tAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCaa                                                                              >  2:600830/1‑150 (MQ=255)
                                                                         aacaacGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAAtt                                                                            <  1:1642717/150‑1 (MQ=255)
                                                                          acaatGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACTATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAAttt                                                                           <  2:1812392/150‑1 (MQ=255)
                                                                          acaacGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAAttt                                                                           <  2:2271061/150‑1 (MQ=255)
                                                                          acaacGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAAttt                                                                           <  1:3409329/150‑1 (MQ=255)
                                                                          acaacGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAAttt                                                                           <  1:3913568/150‑1 (MQ=255)
                                                                           caacGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACTATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTg                                                                          <  1:895773/150‑1 (MQ=255)
                                                                           caacGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTg                                                                          >  2:4262485/1‑150 (MQ=255)
                                                                               ggAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACTATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCg                                                                      <  1:2484127/150‑1 (MQ=255)
                                                                                 aaTTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACTATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAg                                                                    >  1:3205425/1‑150 (MQ=255)
                                                                                 aaTTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAg                                                                    <  1:2537062/150‑1 (MQ=255)
                                                                                  aTTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGt                                                                   <  2:2512011/150‑1 (MQ=255)
                                                                                  aTTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGt                                                                   >  2:3474699/1‑150 (MQ=255)
                                                                                   ttAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACTATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTa                                                                  <  1:2234135/150‑1 (MQ=255)
                                                                                    tAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTAc                                                                 >  2:3154838/1‑150 (MQ=255)
                                                                                     aaCGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTAcc                                                                >  1:1259935/1‑150 (MQ=255)
                                                                                      aCGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCa                                                               >  1:1568039/1‑150 (MQ=255)
                                                                                      aCGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCa                                                               <  1:4087317/150‑1 (MQ=255)
                                                                                         gTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCg                                                            >  2:113425/1‑150 (MQ=255)
                                                                                             aGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGc                                                        >  2:1729929/1‑150 (MQ=255)
                                                                                             aGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGc                                                        <  1:2151642/150‑1 (MQ=255)
                                                                                               tGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGccc                                                      >  1:3556776/1‑150 (MQ=255)
                                                                                                gCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCa                                                     <  2:3589817/150‑1 (MQ=255)
                                                                                                gCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCa                                                     <  1:84031/150‑1 (MQ=255)
                                                                                                    agcagAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTc                                                 >  2:1688676/1‑150 (MQ=255)
                                                                                                      cagAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTccc                                               <  1:3320415/150‑1 (MQ=255)
                                                                                                       agAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTcccc                                              >  1:3749338/1‑150 (MQ=255)
                                                                                                        gAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCg                                             <  1:1020829/150‑1 (MQ=255)
                                                                                                           tGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAg                                          >  2:724740/1‑150 (MQ=255)
                                                                                                            gAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACTATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAgg                                         <  2:3312695/150‑1 (MQ=255)
                                                                                                            gAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAgg                                         <  1:205780/150‑1 (MQ=255)
                                                                                                               cgccgcAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGttt                                      <  1:3814422/150‑1 (MQ=255)
                                                                                                                gccgcAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTa                                     >  1:1327164/1‑150 (MQ=255)
                                                                                                                 ccgcAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTAc                                    <  1:1048380/150‑1 (MQ=255)
                                                                                                                  cgcAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTAcc                                   >  2:2936905/1‑150 (MQ=255)
                                                                                                                   gcAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTAccc                                  <  2:3092072/150‑1 (MQ=255)
                                                                                                                   gcAGCCCAGATAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTAccc                                  <  2:2370087/150‑1 (MQ=255)
                                                                                                                    cAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCt                                 <  1:1015091/150‑1 (MQ=255)
                                                                                                                     aGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTg                                >  1:750588/1‑150 (MQ=255)
                                                                                                                     aGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTg                                >  1:1122/1‑150 (MQ=255)
                                                                                                                        ccAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAAt                             >  2:1232109/1‑150 (MQ=255)
                                                                                                                          aGTTAGAGGGGAAGATTGCCACCACTATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAATcc                           <  2:170438/150‑1 (MQ=255)
                                                                                                                          aGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAATcc                           <  2:2118586/150‑1 (MQ=255)
                                                                                                                             tAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAATCCACg                        <  1:1517060/150‑1 (MQ=255)
                                                                                                                               gagGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAATCCACGCt                      >  2:1498971/1‑150 (MQ=255)
                                                                                                                                agGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAATCCACGCTg                     <  1:4037869/150‑1 (MQ=255)
                                                                                                                                agGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAATCCACGCTg                     >  1:915432/1‑150 (MQ=255)
                                                                                                                                  gggAAGATTGCCACCACTATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAATCCACGCTGGc                   <  2:2599099/150‑1 (MQ=255)
                                                                                                                                      aGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAATCCACGCTGGCCCTg               >  1:2737159/1‑150 (MQ=255)
                                                                                                                                         ttGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGACAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAATCCACGCTGGCCCTGCtt            <  2:1185656/150‑1 (MQ=255)
                                                                                                                                              accacTATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAATCCACGCTGGCCCTGCTTGTTGc       <  2:2565437/150‑1 (MQ=255)
                                                                                                                                               ccacTATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAATCCACGCTGGCCCTGCTTGTTGCa      <  1:4223483/150‑1 (MQ=255)
                                                                                                                                                cacaATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAATCCACGCTGGCCCTGCTTGTTGCAg     <  1:1375707/150‑1 (MQ=255)
                                                                                                                                                   aaTCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAATCCACGCTGGCCCTGCTTGTTGCAGACg  <  1:1928374/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTGAATCCACGCTGGCCCTGCTTGTTGCAGACG  >  CP009273/925810‑926106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A