Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F142 I0 R1	859	274.6	8723680	96.4%	8409627	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,194,194	C→A	32.9%	S15* (TCA→TAA)	lit →	e14 prophage; cell death peptidase, inhibitor of T4 late gene expression

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,194,194	0	C	A	32.9%	156.4 / 83.4	94	S15* (TCA→TAA)	lit	e14 prophage; cell death peptidase, inhibitor of T4 late gene expression
Reads supporting (aligned to +/- strand): ref base C (32/31); new base A (21/10); total (53/41)
Fisher's exact test for biased strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATAATTCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAGTTCTAACA  >  CP009273/1194050‑1194342

cGATAATTCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCAt                                                                                                                                                 >  2:1105310/1‑150 (MQ=255)

   tAATTCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTa                                                                                                                                              <  2:4044213/150‑1 (MQ=255)

   tAATTCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTa                                                                                                                                              >  1:688765/1‑150 (MQ=255)

    aaTTCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTaa                                                                                                                                             >  2:82536/1‑150 (MQ=255)

    aaTTCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAAACATTTaa                                                                                                                                             >  2:83256/1‑150 (MQ=255)

           tACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGTTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACAATTTAAGATTGCa                                                                                                                                      >  1:2689570/1‑150 (MQ=255)

           tACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCa                                                                                                                                      >  1:2689748/1‑150 (MQ=255)

             cGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATCCATCACCATTTAAGATTGCAcc                                                                                                                                    <  2:3330232/150‑1 (MQ=255)

               cATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCag                                                                                                                                  >  1:2923062/1‑150 (MQ=255)

                 tataGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCagag                                                                                                                                >  1:3566529/1‑150 (MQ=255)

                       aTCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAg                                                                                                                          >  2:2317695/1‑150 (MQ=255)

                       aTCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAg                                                                                                                          >  2:143162/1‑150 (MQ=255)

                         cGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCa                                                                                                                        <  2:2966077/150‑1 (MQ=255)

                         cGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCa                                                                                                                        <  2:2966761/150‑1 (MQ=255)

                         cGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCa                                                                                                                        <  2:2966734/150‑1 (MQ=255)

                         cGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTAAGCAATTAAATCATAAACATTTAAGATAGCACCAGAGAATGAgaa                                                                                                                        >  1:3271360/1‑148 (MQ=255)

                         cGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAAGATGTCATCTTTTCTCAGCAATTAAATCATAAACATTTAAGATTGCACCAGAGAAGGAGCa                                                                                                                        >  1:1442933/1‑150 (MQ=255)

                          gAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCaa                                                                                                                       <  2:491211/150‑1 (MQ=255)

                               tAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCt                                                                                                                  <  2:608100/150‑1 (MQ=255)

                                 gAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCACGATCTTa                                                                                                                >  1:770349/1‑150 (MQ=255)

                                  aaaaaaCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTaa                                                                                                               >  1:1695666/1‑150 (MQ=255)

                                     aaaCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAAc                                                                                                            <  2:2923062/150‑1 (MQ=255)

                                      aaCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACg                                                                                                           >  2:3255326/1‑150 (MQ=255)

                                      aaCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACg                                                                                                           <  1:2957071/150‑1 (MQ=255)

                                         tGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATa                                                                                                        <  2:1232846/150‑1 (MQ=255)

                                         tGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATa                                                                                                        <  2:3916652/150‑1 (MQ=255)

                                         tGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATa                                                                                                        <  2:3916737/150‑1 (MQ=255)

                                         tGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATa                                                                                                        <  2:69069/150‑1 (MQ=255)

                                           aaGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGt                                                                                                      <  1:1009819/150‑1 (MQ=255)

                                           aaGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGt                                                                                                      >  2:1056384/1‑150 (MQ=255)

                                            aGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGtt                                                                                                     <  1:1527528/150‑1 (MQ=255)

                                             gATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTg                                                                                                    <  2:2203449/150‑1 (MQ=255)

                                              aTTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTga                                                                                                   >  2:2024170/1‑150 (MQ=255)

                                                 cATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTgacga                                                                                                >  2:3891427/1‑150 (MQ=255)

                                                 cATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTgacga                                                                                                >  2:3891561/1‑150 (MQ=255)

                                                      tATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACaaaa                                                                                           <  2:3574883/150‑1 (MQ=255)

                                                       aTTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACaaaaa                                                                                          >  1:1353314/1‑150 (MQ=255)

                                                          ttttatataCTACCTAGCCCAACACTGTAGAGGTTAACGAAAAATGCGCTCAACAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAAt                                                                                       <  1:2220374/147‑1 (MQ=255)

                                                          ttGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAAt                                                                                       <  1:2220640/150‑1 (MQ=255)

                                                          ttGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAAt                                                                                       <  1:2220304/150‑1 (MQ=255)

                                                               tataCTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATaa                                                                                  >  1:2750832/1‑150 (MQ=255)

                                                               tataCTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATaa                                                                                  >  1:2750844/1‑150 (MQ=255)

                                                                  actacCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAAttt                                                                               <  2:3775176/150‑1 (MQ=255)

                                                                   ctacCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTc                                                                              >  2:973300/1‑150 (MQ=255)

                                                                        tAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAACAAATTATAATTTCAGTtg                                                                         >  1:3345322/1‑150 (MQ=255)

                                                                        tAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtg                                                                         >  1:924476/1‑150 (MQ=255)

                                                                        tAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtg                                                                         >  1:557022/1‑150 (MQ=255)

                                                                        tAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtg                                                                         >  1:556902/1‑150 (MQ=255)

                                                                        tAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtg                                                                         >  1:1150252/1‑150 (MQ=255)

                                                                        tAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtg                                                                         <  1:2084837/150‑1 (MQ=255)

                                                                        tAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtg                                                                         >  1:556900/1‑150 (MQ=255)

                                                                        tAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtg                                                                         >  1:556989/1‑150 (MQ=255)

                                                                        tAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtg                                                                         >  1:583760/1‑150 (MQ=255)

                                                                        tAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtg                                                                         >  1:957618/1‑150 (MQ=255)

                                                                        tAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtg                                                                         >  1:583710/1‑150 (MQ=255)

                                                                         aGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtgt                                                                        >  1:3250637/1‑150 (MQ=255)

                                                                         aGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtgt                                                                        >  1:3251552/1‑150 (MQ=255)

                                                                          gCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtgtg                                                                       <  1:742834/150‑1 (MQ=255)

                                                                          gCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtgtg                                                                       <  1:1385706/150‑1 (MQ=255)

                                                                          gCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTtgtg                                                                       <  1:3077418/150‑1 (MQ=255)

                                                                            acaacaaTTTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGAAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTgtgag                                                                     <  2:1543824/149‑1 (MQ=255)

                                                                              aacaaTGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTgtgagtg                                                                   >  2:1669303/1‑150 (MQ=255)

                                                                               acaaTGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTgtgagtga                                                                  <  1:5098/150‑1 (MQ=255)

                                                                               acaaTGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTgtgagtga                                                                  <  1:1521471/150‑1 (MQ=255)

                                                                                 aaTGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAAc                                                                >  1:463857/1‑150 (MQ=255)

                                                                                  aTGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTAATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATAGCACCAGAGAAGGAGCAAGAACTTAAAACGATAGTTTACGACAAAAAAATTATAATTTCAGTTGTGAGTGAAcc                                                               >  2:2901496/1‑150 (MQ=255)

                                                                                   tGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCt                                                              <  2:116826/150‑1 (MQ=255)

                                                                                           ttAACGAAAAATGCGCTCACCAAGTTGTCATCGTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGAGAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTaa                                                      <  1:3636844/150‑1 (MQ=255)

                                                                                               cGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATc                                                  >  1:3889950/1‑150 (MQ=255)

                                                                                               cGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATc                                                  <  1:3033953/150‑1 (MQ=255)

                                                                                                      tGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCa                                           <  1:973300/150‑1 (MQ=255)

                                                                                                         gcTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGga                                        >  2:1857790/1‑150 (MQ=255)

                                                                                                           tCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGgaag                                      <  2:957809/150‑1 (MQ=255)

                                                                                                           tCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGgaag                                      <  2:957806/150‑1 (MQ=255)

                                                                                                           tCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGgaag                                      <  2:477552/150‑1 (MQ=255)

                                                                                                           tCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGgaag                                      <  1:1983400/150‑1 (MQ=255)

                                                                                                           tCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGgaag                                      <  1:1983011/150‑1 (MQ=255)

                                                                                                                 aTTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATgag                                >  1:1286340/1‑150 (MQ=255)

                                                                                                                 aTTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATgag                                >  1:1286730/1‑150 (MQ=255)

                                                                                                                  tttGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATgaga                               <  1:3644309/150‑1 (MQ=255)

                                                                                                                    tGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGt                             >  2:1533474/1‑150 (MQ=255)

                                                                                                                       cATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGtaat                          >  1:3363377/1‑150 (MQ=255)

                                                                                                                       cATCTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGtaat                          >  1:1218886/1‑150 (MQ=255)

                                                                                                                        aTCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGtaata                         >  1:4050563/1‑150 (MQ=255)

                                                                                                                          cTTTTCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTaataat                       >  2:1117766/1‑150 (MQ=255)

                                                                                                                             ttCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTca                    >  1:1736991/1‑150 (MQ=255)

                                                                                                                             ttCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTca                    >  1:1736975/1‑150 (MQ=255)

                                                                                                                             ttCTCAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTca                    >  1:3385052/1‑150 (MQ=255)

                                                                                                                              tctcAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTcac                   <  1:3242465/150‑1 (MQ=255)

                                                                                                                               ctcAGCAATTAATTCATAACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTcaca                  >  1:2415597/1‑150 (MQ=255)

                                                                                                                                  aGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTGGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGa               >  2:280485/1‑150 (MQ=255)

                                                                                                                                     aattaattCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAAt            >  1:2870561/1‑150 (MQ=255)

                                                                                                                                       ttaattCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAg          <  1:2317695/150‑1 (MQ=255)

                                                                                                                                       ttaattCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAg          <  1:143162/150‑1 (MQ=255)

                                                                                                                                              atcaCCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAGTTCTAAc   <  2:1019416/150‑1 (MQ=255)

                                                                                                                                               tcaCCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAGTTCTAACa  <  2:1287491/150‑1 (MQ=255)

CGATAATTCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAGTTCTAACA  >  CP009273/1194050‑1194342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A