Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,204,284 C→A 15.3% D98Y (GAT→TAT)  tfaE ← e14 prophage; putative tail fiber assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,204,2840CA15.3% 355.2 / 39.5 116D98Y (GAT→TAT) tfaEe14 prophage; putative tail fiber assembly protein
Reads supporting (aligned to +/- strand):  ref base C (44/54);  new base A (7/11);  total (51/65)
Fisher's exact test for biased strand distribution p-value = 7.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

GCAGCATCAATCAGTGACTGGCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAGGGGCATCTAAACAGG  >  CP009273/1204139‑1204433
                                                                                                                                                 |                                                                                                                                                     
   gcaTCAATCAGTGACTGGCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTGGTATTTTCAGGGTAATCACCCGGa                                                                                                                                                >  1:1534568/1‑150 (MQ=255)
      tcaatcaGTGACTGGCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCt                                                                                                                                             >  1:4156288/1‑150 (MQ=255)
        aatcaGTGACTGGCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCttt                                                                                                                                           >  1:2102323/1‑150 (MQ=255)
            aGTGACTGGCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGAtt                                                                                                                                       >  1:1269643/1‑150 (MQ=255)
            aGTGACTGGCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGAtt                                                                                                                                       >  1:1270159/1‑150 (MQ=255)
                 cTGGCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCttt                                                                                                                                  <  2:20362/150‑1 (MQ=255)
                 cTGGCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCttt                                                                                                                                  <  2:2453503/150‑1 (MQ=255)
                   ggCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTg                                                                                                                                >  1:227957/1‑150 (MQ=255)
                   ggCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTg                                                                                                                                >  1:227787/1‑150 (MQ=255)
                   ggCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTg                                                                                                                                >  1:227701/1‑150 (MQ=255)
                   ggCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTg                                                                                                                                >  2:960619/1‑150 (MQ=255)
                     cgcTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGAt                                                                                                                              >  2:3583278/1‑150 (MQ=255)
                         gtgCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATtctc                                                                                                                          >  1:2492730/1‑150 (MQ=255)
                         gtgCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATtctc                                                                                                                          >  1:2492133/1‑150 (MQ=255)
                            cTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAg                                                                                                                       <  2:3892176/150‑1 (MQ=255)
                            cTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAg                                                                                                                       >  1:3181158/1‑150 (MQ=255)
                            cTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAg                                                                                                                       <  2:3149629/150‑1 (MQ=255)
                            cTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAg                                                                                                                       <  2:501122/150‑1 (MQ=255)
                            cTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAg                                                                                                                       <  2:503423/150‑1 (MQ=255)
                            cTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGAGAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAg                                                                                                                       >  1:990270/1‑150 (MQ=255)
                             ttCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGt                                                                                                                      >  1:682274/1‑150 (MQ=255)
                                tGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTGTGATTTCTTTTGATTATCCAGtttt                                                                                                                   >  1:421549/1‑150 (MQ=255)
                                tGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCGTTGATTTCTTTTGATTCTCCAGtttt                                                                                                                   >  1:421504/1‑150 (MQ=255)
                                  ccGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTgg                                                                                                                 <  2:930147/150‑1 (MQ=255)
                                  ccGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTgg                                                                                                                 <  2:930344/150‑1 (MQ=255)
                                   cgcgTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGt                                                                                                                <  1:4200818/150‑1 (MQ=255)
                                     cgTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATTGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGc                                                                                                              <  2:1680406/150‑1 (MQ=255)
                                       tCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCta                                                                                                            >  1:1583370/1‑150 (MQ=255)
                                       tCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCta                                                                                                            >  2:3022523/1‑150 (MQ=255)
                                          aCTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAg                                                                                                         >  2:2875226/1‑150 (MQ=255)
                                                 cgcTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGttt                                                                                                  <  2:3495122/150‑1 (MQ=255)
                                                 cgcTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGttt                                                                                                  >  1:2279829/1‑150 (MQ=255)
                                                  gcTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTc                                                                                                 <  1:2236100/150‑1 (MQ=255)
                                                    tATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCAc                                                                                               >  1:3533534/1‑150 (MQ=255)
                                                       gCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACcgc                                                                                            <  1:3223981/150‑1 (MQ=255)
                                                        cTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACcgcg                                                                                           >  1:3602818/1‑150 (MQ=255)
                                                         tgtgCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGa                                                                                          >  2:2668455/1‑150 (MQ=255)
                                                          gtgCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGAt                                                                                         <  1:1720173/150‑1 (MQ=255)
                                                           tgCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATg                                                                                        <  1:3849877/150‑1 (MQ=255)
                                                           tgCTTCAGTATCGGTCACCCATTTCTCACCATCCCATGTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATg                                                                                        <  1:765100/150‑1 (MQ=255)
                                                            gCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATgg                                                                                       <  1:3583278/150‑1 (MQ=255)
                                                             cgtCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGt                                                                                      >  2:3135806/3‑150 (MQ=255)
                                                             cTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGt                                                                                      >  2:3135816/1‑150 (MQ=255)
                                                               tCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTCATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCt                                                                                    <  2:3749727/150‑1 (MQ=255)
                                                               tCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCt                                                                                    <  1:2965000/150‑1 (MQ=255)
                                                               tCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCt                                                                                    <  1:1506902/150‑1 (MQ=255)
                                                                cAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTg                                                                                   <  2:4331873/150‑1 (MQ=255)
                                                                cAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTg                                                                                   <  2:467473/150‑1 (MQ=255)
                                                                cAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTg                                                                                   >  2:4103080/1‑150 (MQ=255)
                                                                     tCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGcaca                                                                              <  2:1150756/150‑1 (MQ=255)
                                                                      cGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACat                                                                             <  2:1075585/150‑1 (MQ=255)
                                                                        gTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACatat                                                                           <  1:3569524/150‑1 (MQ=255)
                                                                        gTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACatat                                                                           <  2:2399296/150‑1 (MQ=255)
                                                                         tCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATAtt                                                                          >  2:3927724/1‑150 (MQ=255)
                                                                              cATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCAt                                                                     <  1:2872887/150‑1 (MQ=255)
                                                                               aTTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATg                                                                    <  2:345467/150‑1 (MQ=255)
                                                                                 ttCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAg                                                                  <  1:1634497/150‑1 (MQ=255)
                                                                                   ctcACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGtt                                                                <  1:2424261/150‑1 (MQ=255)
                                                                                    tcACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTa                                                               <  1:4240603/150‑1 (MQ=255)
                                                                                      aCCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTaaa                                                             >  1:815161/1‑150 (MQ=255)
                                                                                        aaTCCCATTTATCGTTTGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGTGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAAt                                                           <  2:3746155/149‑1 (MQ=255)
                                                                                         aTCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATc                                                          <  2:4335727/150‑1 (MQ=255)
                                                                                          tCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCt                                                         >  1:3085963/1‑150 (MQ=255)
                                                                                          tCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCt                                                         <  1:514111/150‑1 (MQ=255)
                                                                                          tCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCt                                                         >  1:3407680/1‑150 (MQ=255)
                                                                                             cATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCa                                                      <  1:3508847/150‑1 (MQ=255)
                                                                                              aTTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAg                                                     <  1:1322694/150‑1 (MQ=255)
                                                                                              aTTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAg                                                     >  2:3037635/1‑150 (MQ=255)
                                                                                                  aTCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACg                                                 <  2:1767586/150‑1 (MQ=255)
                                                                                                    cGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGc                                               <  2:1129632/150‑1 (MQ=255)
                                                                                                      tATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAg                                             >  2:879646/1‑150 (MQ=255)
                                                                                                        tGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGAt                                           >  1:4075196/1‑150 (MQ=255)
                                                                                                         ggAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGAtt                                          <  1:1812737/150‑1 (MQ=255)
                                                                                                         ggAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGAtt                                          <  1:1812761/150‑1 (MQ=255)
                                                                                                          gagaTAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTg                                         >  2:3437891/1‑150 (MQ=255)
                                                                                                                aaGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAAc                                   <  2:190842/150‑1 (MQ=255)
                                                                                                                  ggggCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCa                                 >  1:2118066/1‑150 (MQ=255)
                                                                                                                   gggCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAg                                <  1:713983/150‑1 (MQ=255)
                                                                                                                   gggCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAg                                <  1:714012/150‑1 (MQ=255)
                                                                                                                    ggCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGc                               <  2:2568404/150‑1 (MQ=255)
                                                                                                                          aGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTAtg                         >  2:594109/1‑150 (MQ=255)
                                                                                                                          aGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAAACAGCCTTAtg                         >  1:1238291/1‑150 (MQ=255)
                                                                                                                          aGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTATCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTAtg                         >  1:1238279/1‑150 (MQ=255)
                                                                                                                                tGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTAcc                   <  2:1775724/150‑1 (MQ=255)
                                                                                                                                 gTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCa                  <  2:387583/150‑1 (MQ=255)
                                                                                                                                 gTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCa                  <  2:387588/150‑1 (MQ=255)
                                                                                                                                 gTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCa                  <  2:3356843/150‑1 (MQ=255)
                                                                                                                                 gTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCa                  <  2:3356824/150‑1 (MQ=255)
                                                                                                                                 gTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCa                  >  1:1665364/1‑150 (MQ=255)
                                                                                                                                  tATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAg                 <  2:4191638/150‑1 (MQ=255)
                                                                                                                                  tATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAg                 >  2:3819778/1‑150 (MQ=255)
                                                                                                                                  tATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAg                 <  1:960619/150‑1 (MQ=255)
                                                                                                                                   aTTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAgg                >  2:1106251/1‑150 (MQ=255)
                                                                                                                                    ttttCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAggg               <  2:351785/150‑1 (MQ=255)
                                                                                                                                         aGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAGGGGCATc          >  2:2271941/1‑150 (MQ=255)
                                                                                                                                         aGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAAACAGCCTTATGTGTACCAGGGGCATc          >  2:2271956/1‑150 (MQ=255)
                                                                                                                                           ggTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAGGGGCATCTa        <  2:196627/150‑1 (MQ=255)
                                                                                                                                              aaTCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAGGGGCATCTAAAc     >  2:1828888/1‑150 (MQ=255)
                                                                                                                                               aTCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAGGGGCATCTAAACa    <  1:1179015/150‑1 (MQ=255)
                                                                                                                                                 cACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAGGGGCATCTAAACAgg  <  1:4103080/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GCAGCATCAATCAGTGACTGGCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAGGGGCATCTAAACAGG  >  CP009273/1204139‑1204433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A