Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,952,658 C→T 15.8% D443D (GAC→GAT)  ilvC → ketol‑acid reductoisomerase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,952,6580CT15.8% 837.7 / 122.2 298D443D (GAC→GAT) ilvCketol‑acid reductoisomerase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (126/125);  new base T (23/24);  total (149/149)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 305 total aligned reads displayed.

TGCCGCTGATTGCCAACACCATCGCCCGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGT  >  CP009273/3952510‑3952807
                                                                                                                                                    |                                                                                                                                                     
tGCCGCTGATTGCCAACACCATCGCCCGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGAcc                                                                                                                                                      >  1:933268/1‑150 (MQ=255)
tGCCGCTGATTGCCAACACCATCGCCCGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGAcc                                                                                                                                                      >  2:1062635/1‑150 (MQ=255)
tGCCGCTGATTGCCAACACCATCGCCCGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGAcc                                                                                                                                                      >  2:1602777/1‑150 (MQ=255)
tGCCGCTGATTGCCAACACCATCGCCCGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGAcc                                                                                                                                                      >  1:1861107/1‑150 (MQ=255)
  ccGCTGATTGCCAACACCATCGCCCGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTg                                                                                                                                                    >  2:3866986/1‑150 (MQ=255)
      tGATTGCCAACACCATCGCCCGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTa                                                                                                                                                >  2:27220/1‑150 (MQ=255)
        aTTGCCAACACCATCGCCCGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTaaa                                                                                                                                              >  1:439315/1‑150 (MQ=255)
        aTTGCCAACACCATCGCCCGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTaaa                                                                                                                                              >  1:3763253/1‑150 (MQ=255)
         ttGCCAACACCATCGCCCGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAg                                                                                                                                             >  2:3299574/1‑150 (MQ=255)
          tGCCAACACCATCGCCCGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGc                                                                                                                                            >  2:3708074/1‑150 (MQ=255)
              aaCACCCTCGCCCGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTAtt                                                                                                                                        <  1:2405164/150‑1 (MQ=255)
               acacCATCGCCCGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTc                                                                                                                                       <  2:505915/150‑1 (MQ=255)
                   cATCGCCCGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTGTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTCTTCCGGa                                                                                                                                   >  1:778223/1‑150 (MQ=255)
                        cccGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGgcg                                                                                                                              >  1:2135063/1‑150 (MQ=255)
                          cGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGcgcg                                                                                                                            <  2:3435775/150‑1 (MQ=255)
                                   cTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAAc                                                                                                                   <  1:3344917/150‑1 (MQ=255)
                                    tGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACg                                                                                                                  <  2:147244/150‑1 (MQ=255)
                                      tACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACggg                                                                                                                <  1:521500/150‑1 (MQ=255)
                                      tACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACggg                                                                                                                <  1:1143236/150‑1 (MQ=255)
                                        cGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCGTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCa                                                                                                              >  1:3636095/1‑150 (MQ=255)
                                         gAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCaa                                                                                                             >  2:610418/1‑150 (MQ=255)
                                           aaTGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACt                                                                                                           >  1:3221396/1‑150 (MQ=255)
                                              gAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCg                                                                                                        >  2:2169933/1‑150 (MQ=255)
                                              gAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCg                                                                                                        >  2:2824724/1‑150 (MQ=255)
                                               aaCGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGt                                                                                                       >  2:4341627/1‑150 (MQ=255)
                                               aaCGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGt                                                                                                       >  1:3398720/1‑150 (MQ=255)
                                                aCGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTg                                                                                                      <  2:4214524/150‑1 (MQ=255)
                                                 cGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGa                                                                                                     >  2:2960499/1‑150 (MQ=255)
                                                 cGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTCTTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGa                                                                                                     >  1:1090842/1‑150 (MQ=255)
                                                    ggTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGAtgt                                                                                                  >  1:2949959/1‑150 (MQ=255)
                                                     gTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGAtgtg                                                                                                 >  2:135457/1‑150 (MQ=255)
                                                     gTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGAtgtg                                                                                                 >  2:3087844/1‑150 (MQ=255)
                                                           tctGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTCTTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGaa                                                                                           >  2:86932/1‑150 (MQ=255)
                                                             tGATTCCGCTGAGTTCGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAATCCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGc                                                                                         <  1:2139532/150‑1 (MQ=255)
                                                              gATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCg                                                                                        >  2:689243/1‑150 (MQ=255)
                                                              gATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCg                                                                                        >  2:2859731/1‑150 (MQ=255)
                                                               aTACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGACCGAAGCGa                                                                                       >  1:1343709/1‑150 (MQ=255)
                                                               aTACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGa                                                                                       <  1:3380571/150‑1 (MQ=255)
                                                                  ccGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTc                                                                                    <  2:3067004/150‑1 (MQ=255)
                                                                       gAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGc                                                                               >  2:3022393/1‑150 (MQ=255)
                                                                          tACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCAt                                                                            >  2:1869408/1‑150 (MQ=255)
                                                                            cGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGc                                                                          >  2:3191306/1‑150 (MQ=255)
                                                                             ggTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCg                                                                         >  1:2048330/1‑150 (MQ=255)
                                                                             ggTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCg                                                                         >  2:4309330/1‑150 (MQ=255)
                                                                                 aCTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTg                                                                     >  1:4043436/1‑150 (MQ=255)
                                                                                  cTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGa                                                                    >  2:2615521/1‑150 (MQ=255)
                                                                                       tGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCagg                                                               >  2:2290501/1‑150 (MQ=255)
                                                                                          tctcTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCaggtag                                                            <  2:2332767/150‑1 (MQ=255)
                                                                                           ctctTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCaggtagg                                                           >  2:3129008/1‑150 (MQ=255)
                                                                                           ctctTACGCTTGTGTGCCGGTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCaggtagg                                                           <  1:4135973/150‑1 (MQ=255)
                                                                                            tctTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCaggtaggt                                                          <  2:3628192/150‑1 (MQ=255)
                                                                                            tctTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCaggtaggt                                                          <  2:933268/150‑1 (MQ=255)
                                                                                              ttACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTaa                                                        <  1:2831606/150‑1 (MQ=255)
                                                                                              ttACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTaa                                                        <  1:1545890/150‑1 (MQ=255)
                                                                                              ttACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTaa                                                        >  2:1821115/1‑150 (MQ=255)
                                                                                              ttACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTaa                                                        <  1:2189137/150‑1 (MQ=255)
                                                                                                aCGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGa                                                      >  1:3426625/1‑150 (MQ=255)
                                                                                                aCGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGa                                                      >  1:3427624/1‑150 (MQ=255)
                                                                                                 cGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGaa                                                     >  1:449585/1‑150 (MQ=255)
                                                                                                    ttGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACt                                                  <  1:4152158/150‑1 (MQ=255)
                                                                                                    ttGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACt                                                  <  1:2713389/150‑1 (MQ=255)
                                                                                                      gtgtgCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTgc                                                >  2:202250/1‑150 (MQ=255)
                                                                                                       tgtgCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTgcg                                               <  2:2654920/150‑1 (MQ=255)
                                                                                                         tgCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGcgcg                                             <  2:4111220/150‑1 (MQ=255)
                                                                                                           ccGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGc                                           <  2:3340311/150‑1 (MQ=255)
                                                                                                             gTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCta                                         >  2:2435857/1‑150 (MQ=255)
                                                                                                               tGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCtata                                       >  2:565939/1‑150 (MQ=255)
                                                                                                               tGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCtata                                       <  1:4291049/150‑1 (MQ=255)
                                                                                                                gCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTatat                                      <  1:1697887/150‑1 (MQ=255)
                                                                                                                  tGAAACCGTTTCTGGCAGAGCTGCAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGa                                    <  1:1164220/150‑1 (MQ=255)
                                                                                                                  tGAAACCGTTTATGGCAGAGCTGCAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGa                                    <  1:2891165/150‑1 (MQ=255)
                                                                                                                   gAAACCGTTTATGGCAGAGCTGCAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGAc                                   <  2:1988207/150‑1 (MQ=255)
                                                                                                                     aaCCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAg                                 >  1:1025810/1‑150 (MQ=255)
                                                                                                                     aaCCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAg                                 >  1:2327572/1‑150 (MQ=255)
                                                                                                                       ccGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGat                               <  1:23022/150‑1 (MQ=255)
                                                                                                                         gTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGatat                             >  1:1649801/1‑150 (MQ=255)
                                                                                                                         gTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGatat                             <  2:3763253/150‑1 (MQ=255)
                                                                                                                             aTGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGaaa                         <  1:797222/150‑1 (MQ=255)
                                                                                                                             aTGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGaaa                         >  1:1108146/1‑150 (MQ=255)
                                                                                                                             aTGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGaaa                         <  1:3440997/150‑1 (MQ=255)
                                                                                                                              tGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAAc                        <  1:2496329/150‑1 (MQ=255)
                                                                                                                               ggCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACg                       <  1:4292736/150‑1 (MQ=255)
                                                                                                                                 cAGAGCTGCAACCGGGCGATCTGGGCAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTa                     <  2:3986479/150‑1 (MQ=255)
                                                                                                                                  agagCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTAt                    <  1:1586842/150‑1 (MQ=255)
                                                                                                                                  agagCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTAt                    <  1:2564367/150‑1 (MQ=255)
                                                                                                                                  agagCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTAt                    <  2:1010702/150‑1 (MQ=255)
                                                                                                                                      cTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCt                >  1:3145534/1‑150 (MQ=255)
                                                                                                                                      cTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCt                <  2:3545945/150‑1 (MQ=255)
                                                                                                                                       tGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTg               <  1:3386530/150‑1 (MQ=255)
                                                                                                                                         cAACCGGGCGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTCGGTAAGAAACTGCGCGGCTAGATGACAGATATGAAACGTATTGCTGtt             >  2:2348956/1‑150 (MQ=255)
                                                                                                                                         cAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGtt             <  1:3992935/150‑1 (MQ=255)
                                                                                                                                          aaCCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTg            <  1:3964276/150‑1 (MQ=255)
                                                                                                                                          aaCCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTg            <  1:3802610/150‑1 (MQ=255)
                                                                                                                                             cGGGCGACCTGGGTAAAGCTCTTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCgg         >  1:269206/1‑150 (MQ=255)
                                                                                                                                               ggCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGt       <  1:3293924/150‑1 (MQ=255)
                                                                                                                                                 cGATCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTa     <  1:734695/150‑1 (MQ=255)
                                                                                                                                                 cGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTa     <  1:1428920/150‑1 (MQ=255)
                                                                                                                                                  gACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTaa    <  1:1213895/150‑1 (MQ=255)
                                                                                                                                                  gACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTaa    <  2:861189/150‑1 (MQ=255)
                                                                                                                                                   aCCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAg   >  1:4221917/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGCCGCTGATTGCCAACACCATCGCCCGTAAGCGTCTGTACGAAATGAACGTGGTTATCTCTGATACCGCTGAGTACGGTAACTATCTGTTCTCTTACGCTTGTGTGCCGTTGCTGAAACCGTTTATGGCAGAGCTGCAACCGGGCGACCTGGGTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGT  >  CP009273/3952510‑3952807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A