Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F534 I1 R1	1235	230.2	7611948	95.1%	7238962	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,722,763	C→A	intergenic (‑32/+40)	xylB ← / ← xylA	xylulokinase/D‑xylose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,722,763	0	C	A	100.0%	848.6 / NA	254	intergenic (‑32/+40)	xylB/xylA	xylulokinase/D‑xylose isomerase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (106/148); total (106/148)

Only 100 of 255 total aligned reads displayed.

AGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTG  >  CP009273/3722616‑3722909

  tGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTa                                                                                                                                                <  1:3123344/150‑1 (MQ=255)

  tGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTa                                                                                                                                                <  2:3274467/150‑1 (MQ=255)

  tGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTa                                                                                                                                                <  2:1819624/150‑1 (MQ=255)

    gATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTAcc                                                                                                                                              <  2:475448/150‑1 (MQ=255)

       gCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGAt                                                                                                                                           <  1:2042581/150‑1 (MQ=255)

        cGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATa                                                                                                                                          <  1:3255102/150‑1 (MQ=255)

        cGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATa                                                                                                                                          <  2:2325696/150‑1 (MQ=255)

         gggCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATaa                                                                                                                                         <  1:467777/150‑1 (MQ=255)

         gggCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATaa                                                                                                                                         <  1:2373187/150‑1 (MQ=255)

          ggCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAAc                                                                                                                                        <  2:2855475/150‑1 (MQ=255)

             gcgAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCgg                                                                                                                                     >  2:2739906/1‑150 (MQ=255)

               gAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGc                                                                                                                                   >  1:1544588/1‑150 (MQ=255)

               gAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGc                                                                                                                                   >  2:3552101/1‑150 (MQ=255)

                aaaCGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGcc                                                                                                                                  <  1:2848221/150‑1 (MQ=255)

                aaaCGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGcc                                                                                                                                  <  1:944572/150‑1 (MQ=255)

                 aaCGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCa                                                                                                                                 <  2:1918383/150‑1 (MQ=255)

                  aCGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCaa                                                                                                                                <  1:2964615/150‑1 (MQ=255)

                   cGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAAc                                                                                                                               >  1:434154/1‑150 (MQ=255)

                   cGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAAc                                                                                                                               <  2:1313590/150‑1 (MQ=255)

                        aGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACt                                                                                                                          <  2:1016942/150‑1 (MQ=255)

                         gCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTg                                                                                                                         >  1:1479389/1‑150 (MQ=255)

                             ttCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGcaca                                                                                                                     >  1:772469/1‑150 (MQ=255)

                              tCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAg                                                                                                                    <  2:2087390/150‑1 (MQ=255)

                              tCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAg                                                                                                                    >  2:1153692/1‑150 (MQ=255)

                                cGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGtt                                                                                                                  <  2:1113533/150‑1 (MQ=255)

                                cGTGTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGtt                                                                                                                  <  2:2017799/150‑1 (MQ=255)

                                   ttGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGc                                                                                                               <  2:914660/150‑1 (MQ=255)

                                       gcagcaACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGtt                                                                                                           <  1:3361997/150‑1 (MQ=255)

                                         agcaACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAt                                                                                                         <  2:731567/150‑1 (MQ=255)

                                           caACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAttt                                                                                                       <  1:2641829/150‑1 (MQ=255)

                                           caACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAttt                                                                                                       <  2:1532395/150‑1 (MQ=255)

                                            aaCCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTg                                                                                                      >  1:520811/1‑150 (MQ=255)

                                            aaCCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTg                                                                                                      <  2:1656271/150‑1 (MQ=255)

                                            aaCCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTCAGCCGTTATTTg                                                                                                      >  2:1491625/1‑150 (MQ=255)

                                              ccaccTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTc                                                                                                    <  1:1023943/150‑1 (MQ=255)

                                                   tCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACa                                                                                               >  2:2034559/1‑150 (MQ=255)

                                                    cACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAg                                                                                              <  1:3194384/150‑1 (MQ=255)

                                                       ccTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATa                                                                                           <  1:699359/150‑1 (MQ=255)

                                                         tGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAAt                                                                                         >  2:876838/1‑150 (MQ=255)

                                                           cTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATgg                                                                                       >  2:1856202/1‑150 (MQ=255)

                                                            tCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGt                                                                                      <  1:880735/150‑1 (MQ=255)

                                                             cGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGtt                                                                                     <  2:1363220/150‑1 (MQ=255)

                                                               ttGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTa                                                                                   >  1:169913/1‑150 (MQ=255)

                                                                 gAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTAcc                                                                                 <  1:2428002/150‑1 (MQ=255)

                                                                 gAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTAcc                                                                                 <  1:2739906/150‑1 (MQ=255)

                                                                  aGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCa                                                                                >  1:1337588/1‑150 (MQ=255)

                                                                  aGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCa                                                                                <  1:1299778/150‑1 (MQ=255)

                                                                    cAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGa                                                                              >  1:3507352/1‑150 (MQ=255)

                                                                       aaaaaCTATTACGCCCGAGGTTCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATATGTCGAACAGATAATGGTTTAACAGAttt                                                                           >  2:2710769/4‑150 (MQ=255)

                                                                        aTAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGAtttt                                                                          <  1:3322347/150‑1 (MQ=255)

                                                                        aTAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGAtttt                                                                          <  1:3534/150‑1 (MQ=255)

                                                                        aTAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGAtttt                                                                          <  1:3641/150‑1 (MQ=255)

                                                                            cTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAg                                                                      >  1:2495799/1‑150 (MQ=255)

                                                                            cTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAg                                                                      >  1:2495069/1‑150 (MQ=255)

                                                                               ttACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAgttg                                                                   <  2:379153/150‑1 (MQ=255)

                                                                                tACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAgttgt                                                                  <  2:3315018/150‑1 (MQ=255)

                                                                                 aCGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAgttgtt                                                                 <  1:2666705/150‑1 (MQ=255)

                                                                                   gCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTcc                                                               <  1:1155822/150‑1 (MQ=255)

                                                                                    cccGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCt                                                              <  1:1471215/150‑1 (MQ=255)

                                                                                       gAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGc                                                           >  1:838480/1‑150 (MQ=255)

                                                                                         ggTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGa                                                         >  2:2495586/1‑150 (MQ=255)

                                                                                         ggTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGa                                                         >  1:1603218/1‑150 (MQ=255)

                                                                                              cAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCAct                                                    >  1:384959/1‑150 (MQ=255)

                                                                                              cAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCAct                                                    <  1:1361630/150‑1 (MQ=255)

                                                                                                 gATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTg                                                 >  1:897965/1‑150 (MQ=255)

                                                                                                 gATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTg                                                 >  1:3345615/1‑150 (MQ=255)

                                                                                                    cTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATg                                              <  1:1424192/150‑1 (MQ=255)

                                                                                                      aTCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCa                                            <  2:232098/150‑1 (MQ=255)

                                                                                                        cccGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCAcc                                          >  2:571328/1‑150 (MQ=255)

                                                                                                         ccGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACAGGGCCAACGGACTGCACAGTTAGCCGTTTTTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCg                                         <  2:632196/150‑1 (MQ=255)

                                                                                                            atttaCATATTGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGTTTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGgag                                      <  1:1013745/147‑1 (MQ=255)

                                                                                                            atatACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGgag                                      >  1:2684200/1‑150 (MQ=255)

                                                                                                              ataCATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGAc                                    <  2:1108200/150‑1 (MQ=255)

                                                                                                              ataCATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGAc                                    <  2:1108310/150‑1 (MQ=255)

                                                                                                               taCATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACa                                   >  1:157633/1‑150 (MQ=255)

                                                                                                                 cATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACaaa                                 >  2:1276130/1‑150 (MQ=255)

                                                                                                                     tcgatcgTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAatgat                             >  2:1587957/1‑150 (MQ=255)

                                                                                                                      cgatcgTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAatgatg                            <  1:3392030/150‑1 (MQ=255)

                                                                                                                      cgatcgTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAatgatg                            <  1:2132981/150‑1 (MQ=255)

                                                                                                                       gatcgTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGt                           >  1:2949654/1‑150 (MQ=255)

                                                                                                                       gatcgTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGt                           >  1:1772800/1‑150 (MQ=255)

                                                                                                                        atcgTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGtt                          >  1:287897/1‑150 (MQ=255)

                                                                                                                        atcgTTCCTTAAAAAAATGCCCGGTATAGATACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGtt                          <  2:3093417/150‑1 (MQ=255)

                                                                                                                          cgTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTcc                        >  2:3652870/1‑150 (MQ=255)

                                                                                                                          cgTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTcc                        >  2:2719542/1‑150 (MQ=255)

                                                                                                                          cgTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTcc                        <  1:174921/150‑1 (MQ=255)

                                                                                                                          cgTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTcc                        <  2:2571281/150‑1 (MQ=255)

                                                                                                                          cgTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTCAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTcc                        <  1:1491625/150‑1 (MQ=255)

                                                                                                                           gTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCt                       <  1:2197037/150‑1 (MQ=255)

                                                                                                                            ttCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTg                      >  1:3427160/1‑150 (MQ=255)

                                                                                                                             tCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGa                     <  2:838480/150‑1 (MQ=255)

                                                                                                                              ccTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAg                    >  2:3165982/1‑150 (MQ=255)

                                                                                                                              ccTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAg                    <  2:3543992/150‑1 (MQ=255)

                                                                                                                                   aaaaaaTGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCatat               >  1:1940487/1‑150 (MQ=255)

                                                                                                                                     aaaaTGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATAttt             >  1:1858947/1‑150 (MQ=255)

                                                                                                                                     aaaaTGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATAttt             <  2:520811/150‑1 (MQ=255)

                                                                                                                                      aaaTGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTg            >  2:2085323/1‑150 (MQ=255)

                                                                                                                                            ccGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTaaa      >  2:3051201/1‑150 (MQ=255)

                                                                                                                                             cGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAAt     <  1:1113420/150‑1 (MQ=255)

                                                                                                                                               gTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCt   >  1:564723/1‑150 (MQ=255)

AGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTG  >  CP009273/3722616‑3722909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTG  >  CP009273/3722616‑3722909

AGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGC                                                                                                                                                  <  SRR19973061.916729/150‑1 (MQ=60)

 GTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCT                                                                                                                                                 <  SRR19973061.922377/150‑1 (MQ=60)

 GTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCT                                                                                                                                                 <  SRR19973061.2832262/150‑1 (MQ=60)

  TGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTA                                                                                                                                                <  SRR19973061.3131395/150‑1 (MQ=60)

   GGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTAC                                                                                                                                               >  SRR19973061.884253/1‑150 (MQ=60)

       GCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGAT                                                                                                                                           <  SRR19973061.2047714/150‑1 (MQ=60)

       GCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGAT                                                                                                                                           <  SRR19973061.426941/150‑1 (MQ=60)

        CGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATA                                                                                                                                          >  SRR19973061.1522499/1‑150 (MQ=60)

        CGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATA                                                                                                                                          <  SRR19973061.3263475/150‑1 (MQ=60)

         GGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAA                                                                                                                                         <  SRR19973061.469046/150‑1 (MQ=60)

         GGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAA                                                                                                                                         <  SRR19973061.2379232/150‑1 (MQ=60)

          GGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAAC                                                                                                                                        <  SRR19973061.61318/150‑1 (MQ=60)

          GGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAAC                                                                                                                                        <  SRR19973061.2187969/150‑1 (MQ=60)

               GAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGC                                                                                                                                   >  SRR19973061.1548433/1‑150 (MQ=60)

                AAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCC                                                                                                                                  <  SRR19973061.2855509/150‑1 (MQ=60)

                AAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCC                                                                                                                                  <  SRR19973061.1788249/150‑1 (MQ=60)

                AAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCC                                                                                                                                  <  SRR19973061.2855517/150‑1 (MQ=60)

                AAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCC                                                                                                                                  <  SRR19973061.946967/150‑1 (MQ=60)

                AAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCC                                                                                                                                  <  SRR19973061.946978/150‑1 (MQ=60)

                 AACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCC                                                                                                                                 >  SRR19973061.1445242/1‑150 (MQ=60)

                  ACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAA                                                                                                                                <  SRR19973061.2972240/150‑1 (MQ=60)

                  ACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAA                                                                                                                                <  SRR19973061.2972256/150‑1 (MQ=60)

                  ACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAA                                                                                                                                <  SRR19973061.2972265/150‑1 (MQ=60)

                   CGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAAC                                                                                                                               >  SRR19973061.435339/1‑150 (MQ=60)

                         GCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTG                                                                                                                         <  SRR19973061.2285400/150‑1 (MQ=60)

                         GCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTG                                                                                                                         >  SRR19973061.1483118/1‑150 (MQ=60)

                         GCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTG                                                                                                                         >  SRR19973061.1483101/1‑150 (MQ=60)

                             TTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACA                                                                                                                     >  SRR19973061.774503/1‑150 (MQ=60)

                                     GCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCG                                                                                                             <  SRR19973061.1540338/150‑1 (MQ=60)

                                       GCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTT                                                                                                           <  SRR19973061.3370620/150‑1 (MQ=60)

                                       GCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTT                                                                                                           <  SRR19973061.3370623/150‑1 (MQ=60)

                                       GCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTT                                                                                                           <  SRR19973061.3370642/150‑1 (MQ=60)

                                           CAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTT                                                                                                       <  SRR19973061.2649011/150‑1 (MQ=60)

                                           CAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTT                                                                                                       <  SRR19973061.2648614/150‑1 (MQ=60)

                                            AACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTG                                                                                                      <  SRR19973061.238337/150‑1 (MQ=60)

                                            AACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTG                                                                                                      >  SRR19973061.522184/1‑150 (MQ=60)

                                              CCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTC                                                                                                    <  SRR19973061.2867761/150‑1 (MQ=60)

                                              CCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTC                                                                                                    <  SRR19973061.1026558/150‑1 (MQ=60)

                                               CACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCG                                                                                                   >  SRR19973061.1569949/1‑150 (MQ=60)

                                                   TCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACA                                                                                               <  SRR19973061.3233624/150‑1 (MQ=60)

                                                    CACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAG                                                                                              >  SRR19973061.3397414/1‑150 (MQ=60)

                                                    CACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAG                                                                                              <  SRR19973061.3202585/150‑1 (MQ=60)

                                                      CCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGAT                                                                                            <  SRR19973061.3190927/150‑1 (MQ=60)

                                                       CCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATA                                                                                           <  SRR19973061.701182/150‑1 (MQ=60)

                                                       CCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATA                                                                                           <  SRR19973061.2611740/150‑1 (MQ=60)

                                                           CTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGG                                                                                       <  SRR19973061.1341534/150‑1 (MQ=60)

                                                           CTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGG                                                                                       <  SRR19973061.1341542/150‑1 (MQ=60)

                                                           CTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGG                                                                                       <  SRR19973061.2682811/150‑1 (MQ=60)

                                                           CTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGG                                                                                       <  SRR19973061.772466/150‑1 (MQ=60)

                                                           CTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGG                                                                                       <  SRR19973061.347664/150‑1 (MQ=60)

                                                            TCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGT                                                                                      <  SRR19973061.3008022/150‑1 (MQ=60)

                                                            TCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGT                                                                                      <  SRR19973061.883021/150‑1 (MQ=60)

                                                             CGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTT                                                                                     >  SRR19973061.414144/1‑150 (MQ=60)

                                                              GTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTT                                                                                    <  SRR19973061.110178/150‑1 (MQ=60)

                                                               TTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTA                                                                                   >  SRR19973061.1141717/1‑150 (MQ=60)

                                                               TTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTA                                                                                   >  SRR19973061.170437/1‑150 (MQ=60)

                                                               TTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTA                                                                                   >  SRR19973061.170570/1‑150 (MQ=60)

                                                                 GAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACC                                                                                 <  SRR19973061.2434198/150‑1 (MQ=60)

                                                                 GAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACC                                                                                 <  SRR19973061.2746955/150‑1 (MQ=60)

                                                                 GAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACC                                                                                 <  SRR19973061.2434411/150‑1 (MQ=60)

                                                                  AGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCA                                                                                <  SRR19973061.1303016/150‑1 (MQ=60)

                                                                  AGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCA                                                                                >  SRR19973061.1340954/1‑150 (MQ=60)

                                                                  AGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCA                                                                                >  SRR19973061.864755/1‑150 (MQ=60)

                                                                   GCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAG                                                                               >  SRR19973061.1726486/1‑150 (MQ=60)

                                                                    CAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGA                                                                              >  SRR19973061.2001029/1‑150 (MQ=60)

                                                                    CAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGA                                                                              >  SRR19973061.3516330/1‑150 (MQ=60)

                                                                        ATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTT                                                                          <  SRR19973061.3330912/150‑1 (MQ=60)

                                                                        ATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTT                                                                          <  SRR19973061.3542/150‑1 (MQ=60)

                                                                        ATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTT                                                                          <  SRR19973061.3649/150‑1 (MQ=60)

                                                                        ATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTT                                                                          <  SRR19973061.502873/150‑1 (MQ=60)

                                                                            CTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAG                                                                      >  SRR19973061.799571/1‑150 (MQ=60)

                                                                            CTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAG                                                                      >  SRR19973061.2502155/1‑150 (MQ=60)

                                                                            CTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAG                                                                      >  SRR19973061.2501423/1‑150 (MQ=60)

                                                                             TTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGT                                                                     >  SRR19973061.73927/1‑150 (MQ=60)

                                                                             TTTTACGCCCGAGGTGACAAGATCTATCTCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGT                                                                     <  SRR19973061.2100621/150‑1 (MQ=60)

                                                                                TACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGT                                                                  >  SRR19973061.1588991/1‑150 (MQ=60)

                                                                                 ACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTT                                                                 <  SRR19973061.2673563/150‑1 (MQ=60)

                                                                                 ACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTT                                                                 <  SRR19973061.2673575/150‑1 (MQ=60)

                                                                                  CGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTC                                                                >  SRR19973061.3346515/1‑150 (MQ=60)

                                                                                   GCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCC                                                               <  SRR19973061.1158755/150‑1 (MQ=60)

                                                                                    CCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCT                                                              <  SRR19973061.1475401/150‑1 (MQ=60)

                                                                                    CCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCT                                                              <  SRR19973061.1474911/150‑1 (MQ=60)

                                                                                     CCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTG                                                             <  SRR19973061.2483735/150‑1 (MQ=60)

                                                                                     CCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTG                                                             <  SRR19973061.1484090/150‑1 (MQ=60)

                                                                                       GAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGC                                                           >  SRR19973061.840660/1‑150 (MQ=60)

                                                                                         GGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGA                                                         >  SRR19973061.1607206/1‑150 (MQ=60)

                                                                                            GCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCA                                                      >  SRR19973061.3405232/1‑150 (MQ=60)

                                                                                              CAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACT                                                    >  SRR19973061.1805349/1‑150 (MQ=60)

                                                                                              CAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACT                                                    <  SRR19973061.1365065/150‑1 (MQ=60)

                                                                                              CAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACT                                                    >  SRR19973061.386010/1‑150 (MQ=60)

                                                                                                AGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCT                                                  <  SRR19973061.449360/150‑1 (MQ=60)

                                                                                                AGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCT                                                  >  SRR19973061.3632844/1‑150 (MQ=60)

                                                                                                 GATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTG                                                 >  SRR19973061.900281/1‑150 (MQ=60)

                                                                                                 GATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTG                                                 >  SRR19973061.3354226/1‑150 (MQ=60)

                                                                                                    CTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATG                                              <  SRR19973061.1427780/150‑1 (MQ=60)

                                                                                                    CTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTAGAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATG                                              <  SRR19973061.2726972/150‑1 (MQ=60)

                                                                                                      ATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCA                                            >  SRR19973061.2864418/1‑150 (MQ=60)

                                                                                                       TCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCAC                                           >  SRR19973061.3444972/1‑150 (MQ=60)

                                                                                                        CCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACC                                          <  SRR19973061.2545377/150‑1 (MQ=60)

                                                                                                            ATTTACATATTGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGTTTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAG                                      <  SRR19973061.1016328/150‑1 (MQ=60)

                                                                                                            ATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAG                                      >  SRR19973061.2691108/1‑150 (MQ=60)

                                                                                                            ATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAG                                      <  SRR19973061.1016350/150‑1 (MQ=60)

                                                                                                               TACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACA                                   >  SRR19973061.158125/1‑150 (MQ=60)

                                                                                                               TACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACA                                   <  SRR19973061.3521655/150‑1 (MQ=60)

                                                                                                                ACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAA                                  >  SRR19973061.3329534/1‑150 (MQ=60)

                                                                                                                ACATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAA                                  >  SRR19973061.3329533/1‑150 (MQ=60)

                                                                                                                 CATATCGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAA                                 >  SRR19973061.3809156/1‑150 (MQ=60)

                                                                                                                      CGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATG                            <  SRR19973061.3400743/150‑1 (MQ=60)

                                                                                                                      CGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATG                            <  SRR19973061.2138339/150‑1 (MQ=60)

                                                                                                                      CGATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATG                            <  SRR19973061.3619065/150‑1 (MQ=60)

                                                                                                                       GATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGT                           >  SRR19973061.2957256/1‑150 (MQ=60)

                                                                                                                       GATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGT                           >  SRR19973061.1776079/1‑150 (MQ=60)

                                                                                                                       GATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGT                           >  SRR19973061.1777240/1‑150 (MQ=60)

                                                                                                                        ATCGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTT                          >  SRR19973061.288670/1‑150 (MQ=60)

                                                                                                                          CGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCC                        <  SRR19973061.648557/150‑1 (MQ=60)

                                                                                                                          CGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCC                        <  SRR19973061.175457/150‑1 (MQ=60)

                                                                                                                          CGTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTCAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCC                        <  SRR19973061.1495368/150‑1 (MQ=60)

                                                                                                                           GTTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCT                       <  SRR19973061.2202559/150‑1 (MQ=60)

                                                                                                                           GTTCCTTAAAAAAATGCCCGGTAGAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCT                       <  SRR19973061.145413/150‑1 (MQ=60)

                                                                                                                            TTCCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTG                      >  SRR19973061.3435950/1‑150 (MQ=60)

                                                                                                                              CCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAG                    <  SRR19973061.1306107/150‑1 (MQ=60)

                                                                                                                              CCTTAAAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAG                    <  SRR19973061.879114/150‑1 (MQ=60)

                                                                                                                                   AAAAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATAT               >  SRR19973061.1945316/1‑150 (MQ=60)

                                                                                                                                     AAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTT             >  SRR19973061.1863585/1‑150 (MQ=60)

                                                                                                                                     AAAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTT             >  SRR19973061.2933895/1‑150 (MQ=60)

                                                                                                                                      AAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTG            >  SRR19973061.1199026/1‑150 (MQ=60)

                                                                                                                                      AAATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTG            >  SRR19973061.1199226/1‑150 (MQ=60)

                                                                                                                                       AATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGG           <  SRR19973061.1991322/150‑1 (MQ=60)

                                                                                                                                       AATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGG           <  SRR19973061.3161697/150‑1 (MQ=60)

                                                                                                                                       AATGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGG           <  SRR19973061.1395864/150‑1 (MQ=60)

                                                                                                                                        ATGCCCGGGATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGC          <  SRR19973061.2126876/150‑1 (MQ=60)

                                                                                                                                         TGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCT         <  SRR19973061.3392538/150‑1 (MQ=60)

                                                                                                                                         TGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCT         >  SRR19973061.163233/1‑150 (MQ=60)

                                                                                                                                         TGCCCGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCT         <  SRR19973061.2297276/150‑1 (MQ=60)

                                                                                                                                             CGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAAT     >  SRR19973061.1591991/1‑150 (MQ=60)

                                                                                                                                             CGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAAT     <  SRR19973061.1116269/150‑1 (MQ=60)

                                                                                                                                             CGGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAAT     <  SRR19973061.1039121/150‑1 (MQ=60)

                                                                                                                                              GGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATC    <  SRR19973061.2501941/150‑1 (MQ=60)

                                                                                                                                              GGTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATC    >  SRR19973061.1918754/1‑150 (MQ=60)

                                                                                                                                               GTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCT   >  SRR19973061.566200/1‑150 (MQ=60)

                                                                                                                                               GTATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCT   >  SRR19973061.566097/1‑150 (MQ=60)

                                                                                                                                                TATAGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACACTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTG  >  SRR19973061.520592/1‑150 (MQ=60)

AGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTG  >  CP009273/3722616‑3722909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`