Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,780,573 C→A R126S (CGT→AGT)  envC → activator of AmiB,C murein hydrolases, septal ring factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,780,5730CA99.6% 760.1 / NA 231R126S (CGT→AGT) envCactivator of AmiB,C murein hydrolases, septal ring factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (125/105);  minor base T (0/1);  total (125/106)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

AAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCC  >  CP009273/3780427‑3780719
                                                                                                                                                  |                                                                                                                                                  
 aGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCGTTCAGTCa                                                                                                                                                >  2:841503/1‑150 (MQ=255)
 aGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCa                                                                                                                                                >  1:854477/1‑150 (MQ=255)
 aGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCa                                                                                                                                                >  1:3801587/1‑150 (MQ=255)
  gCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATtcagtcag                                                                                                                                               <  1:56130/150‑1 (MQ=255)
    cACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAggg                                                                                                                                             >  2:2945310/1‑150 (MQ=255)
    cACCCGTAAGCAGCGCGAAACGCAAAACACCCACAATCAACACAAAAAACAGAATGATGAGATGAACGCGTCGAATGCCAAACTCGAGCAGCAAAAAGCCGCCCAGGAACACAGCCTCGCCGCACAACAGGATGCCGCATTCAGTCaaag                                                                                                                                             >  1:785014/1‑147 (MQ=255)
       ccGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGa                                                                                                                                          <  1:2424135/150‑1 (MQ=255)
        cGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAg                                                                                                                                         >  2:2501020/1‑150 (MQ=255)
           aaGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCAt                                                                                                                                      >  1:3499657/1‑150 (MQ=255)
            aGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATa                                                                                                                                     >  1:709055/1‑150 (MQ=255)
              cTGCGCGAAACGCAATACACGCTAATTCTACTTTATATTCAGATTGATGAGTTGAACGCGTCGATTGCCATAATGGAGCAGCATAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATAcc                                                                                                                                   <  1:3658241/150‑1 (MQ=255)
                 cgcgAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGt                                                                                                                                >  1:1374876/1‑150 (MQ=255)
                  gcgAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTa                                                                                                                               <  2:827886/150‑1 (MQ=255)
                   cgAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTAt                                                                                                                              <  2:488642/150‑1 (MQ=255)
                    gAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTAtt                                                                                                                             >  1:687095/1‑150 (MQ=255)
                     aaaCGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTc                                                                                                                            <  2:3515270/150‑1 (MQ=255)
                     aaaCGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTc                                                                                                                            <  2:3328689/150‑1 (MQ=255)
                      aaCGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCa                                                                                                                           <  1:577639/150‑1 (MQ=255)
                       aCGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAg                                                                                                                          <  1:1868055/150‑1 (MQ=255)
                        cGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGc                                                                                                                         >  2:1681787/1‑150 (MQ=255)
                         gCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCCTACCGGAATTCAGCt                                                                                                                        >  1:3410132/1‑150 (MQ=255)
                         gCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCt                                                                                                                        <  2:2606059/150‑1 (MQ=255)
                           aaaaCACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGa                                                                                                                      >  2:1932814/1‑150 (MQ=255)
                            aaaCACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGAt                                                                                                                     <  2:1465091/150‑1 (MQ=255)
                             aaCACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGAtt                                                                                                                    >  2:1980646/1‑150 (MQ=255)
                             aaCACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGAtt                                                                                                                    <  1:1466818/150‑1 (MQ=255)
                                 cGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCa                                                                                                                >  1:3235651/1‑150 (MQ=255)
                                  gCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAg                                                                                                               <  1:3597547/150‑1 (MQ=255)
                                     caatcaaCTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCgg                                                                                                            <  1:896159/150‑1 (MQ=255)
                                      aatcaaCTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGt                                                                                                           >  1:2268802/1‑150 (MQ=255)
                                          aaCTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTgaag                                                                                                       >  2:92923/1‑150 (MQ=255)
                                            cTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTgaagaa                                                                                                     >  2:1222021/1‑150 (MQ=255)
                                             tCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGaaa                                                                                                    <  2:151641/150‑1 (MQ=255)
                                             tCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGaaa                                                                                                    <  2:1021452/150‑1 (MQ=255)
                                               aaTAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGc                                                                                                  >  1:2382494/1‑150 (MQ=255)
                                               aaTAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGc                                                                                                  >  2:2236339/1‑150 (MQ=255)
                                                   aaCAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGc                                                                                              >  1:1083914/1‑150 (MQ=255)
                                                    aCAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCg                                                                                             <  2:737679/150‑1 (MQ=255)
                                                     cAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGt                                                                                            >  2:2031066/1‑150 (MQ=255)
                                                      aGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCCTACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTg                                                                                           >  2:2117369/1‑150 (MQ=255)
                                                        aTTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGa                                                                                         <  1:3728701/150‑1 (MQ=255)
                                                        aTTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGa                                                                                         >  2:1118739/1‑150 (MQ=255)
                                                        aTTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGa                                                                                         >  1:1732059/1‑150 (MQ=255)
                                                          tgatgaGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACa                                                                                       <  1:988705/150‑1 (MQ=255)
                                                           gatgagatgaACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAg                                                                                      >  2:975053/1‑150 (MQ=255)
                                                           gatgagatgaACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAg                                                                                      >  2:975025/1‑150 (MQ=255)
                                                           gatgagatgaACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAg                                                                                      >  1:1398931/1‑150 (MQ=255)
                                                               agatgaACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGtt                                                                                  <  2:3708261/150‑1 (MQ=255)
                                                                       gcgTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCt                                                                          <  2:69014/150‑1 (MQ=255)
                                                                        cgTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCtt                                                                         <  2:1730529/150‑1 (MQ=255)
                                                                         gTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTa                                                                        <  2:1851320/150‑1 (MQ=255)
                                                                         gTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCAGTCAGTCAGGGCGAGCCTACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTa                                                                        >  2:2573560/1‑150 (MQ=255)
                                                                          tCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTAt                                                                       <  2:867321/150‑1 (MQ=255)
                                                                            gATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTAttt                                                                     >  1:1498577/1‑150 (MQ=255)
                                                                            gATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTAttt                                                                     <  2:2488181/150‑1 (MQ=255)
                                                                                  cAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTa                                                               <  1:1253119/150‑1 (MQ=255)
                                                                                     aCTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTAtct                                                            <  2:664302/150‑1 (MQ=255)
                                                                                      cTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTAtctc                                                           <  1:3350723/150‑1 (MQ=255)
                                                                                       tGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCa                                                          >  2:526327/1‑150 (MQ=255)
                                                                                       tGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCa                                                          >  2:526376/1‑150 (MQ=255)
                                                                                        ggAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCTTACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCaa                                                         <  2:2362544/150‑1 (MQ=255)
                                                                                         gAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAAc                                                        <  1:2501020/150‑1 (MQ=255)
                                                                                          agcagcAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAAcc                                                       <  1:2856603/150‑1 (MQ=255)
                                                                                           gcagcaAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCa                                                      <  1:3299000/150‑1 (MQ=255)
                                                                                            cagcaAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAg                                                     >  2:1351595/1‑150 (MQ=255)
                                                                                            cagcaAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAg                                                     <  1:661964/150‑1 (MQ=255)
                                                                                                 aaaaGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGcgcg                                                >  2:2828659/1‑150 (MQ=255)
                                                                                                   aaGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGAc                                              >  2:1297210/1‑150 (MQ=255)
                                                                                                      ccgccCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAg                                           >  1:1570506/1‑150 (MQ=255)
                                                                                                        gccCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGaa                                         >  1:430628/1‑150 (MQ=255)
                                                                                                           cAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAAcc                                      <  1:3782229/150‑1 (MQ=255)
                                                                                                           cAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAAcc                                      <  1:3297239/150‑1 (MQ=255)
                                                                                                            aGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCa                                     >  2:303302/1‑150 (MQ=255)
                                                                                                             ggAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCAt                                    <  2:3767311/150‑1 (MQ=255)
                                                                                                              gAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCAtt                                   <  1:3684465/150‑1 (MQ=255)
                                                                                                              gAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCAtt                                   <  2:3240390/150‑1 (MQ=255)
                                                                                                              gAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCAtt                                   <  1:2227688/150‑1 (MQ=255)
                                                                                                                   cAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCa                              <  1:2301754/150‑1 (MQ=255)
                                                                                                                      ccTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGtt                           >  2:3141113/1‑150 (MQ=255)
                                                                                                                       cTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTg                          >  2:2652893/1‑150 (MQ=255)
                                                                                                                       cTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTg                          >  2:1588063/1‑150 (MQ=255)
                                                                                                                        tCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGa                         <  2:1806369/150‑1 (MQ=255)
                                                                                                                         cgccgcACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGaa                        <  2:2358093/150‑1 (MQ=255)
                                                                                                                           ccgcACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGc                      <  1:2794842/150‑1 (MQ=255)
                                                                                                                            cgcACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCa                     >  2:1393505/1‑150 (MQ=255)
                                                                                                                              cacaACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCaaa                   <  2:3786869/150‑1 (MQ=255)
                                                                                                                              cacaACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCaaa                   >  1:2777374/1‑150 (MQ=255)
                                                                                                                              cacaACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCaaa                   >  2:3479288/1‑150 (MQ=255)
                                                                                                                              cacaACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCaaa                   >  2:591816/1‑150 (MQ=255)
                                                                                                                               acaACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAAc                  <  1:2993755/150‑1 (MQ=255)
                                                                                                                               acaACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAAc                  <  1:2588322/150‑1 (MQ=255)
                                                                                                                                 aaCTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAAcgc                <  1:2597334/150‑1 (MQ=255)
                                                                                                                                  aCTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAAcgcg               >  1:543696/1‑150 (MQ=255)
                                                                                                                                   cTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGt              <  1:3200978/150‑1 (MQ=255)
                                                                                                                                   cTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGt              <  1:2174407/150‑1 (MQ=255)
                                                                                                                                       aTGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTgaag          <  2:243512/150‑1 (MQ=255)
                                                                                                                                           cGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGt      <  1:1532758/150‑1 (MQ=255)
                                                                                                                                            gCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTc     <  1:2031066/150‑1 (MQ=255)
                                                                                                                                             cATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCg    >  1:2485513/1‑150 (MQ=255)
                                                                                                                                               ttCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGcc  <  1:1758924/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCC  >  CP009273/3780427‑3780719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATG  >  CP009273/3780425‑3780722
                                                                                                                                                    |                                                                                                                                                     
TGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACACCTGGATGCCGCATTCAG                                                                                                                                                      >  SRR19973061.2101620/1‑150 (MQ=60)
TGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAG                                                                                                                                                      >  SRR19973061.2102099/1‑150 (MQ=60)
   AGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCA                                                                                                                                                   >  SRR19973061.1148798/1‑150 (MQ=60)
   AGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCA                                                                                                                                                   >  SRR19973061.3811275/1‑150 (MQ=60)
   AGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCA                                                                                                                                                   >  SRR19973061.3811389/1‑150 (MQ=60)
   AGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCA                                                                                                                                                   >  SRR19973061.856699/1‑150 (MQ=60)
    GCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAG                                                                                                                                                  <  SRR19973061.979676/150‑1 (MQ=60)
    GCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAG                                                                                                                                                  <  SRR19973061.56352/150‑1 (MQ=60)
      CACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGG                                                                                                                                                <  SRR19973061.3121751/150‑1 (MQ=60)
       ACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGC                                                                                                                                               >  SRR19973061.244205/1‑150 (MQ=60)
         CCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGA                                                                                                                                             <  SRR19973061.2429315/150‑1 (MQ=60)
         CCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGA                                                                                                                                             <  SRR19973061.2430326/150‑1 (MQ=60)
          CGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAG                                                                                                                                            >  SRR19973061.569676/1‑150 (MQ=60)
             AAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCAT                                                                                                                                         >  SRR19973061.2328521/1‑150 (MQ=60)
             AAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCAT                                                                                                                                         >  SRR19973061.3508602/1‑150 (MQ=60)
              AGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATA                                                                                                                                        >  SRR19973061.710904/1‑150 (MQ=60)
                CTGCGCGAAACGCAATACACGCTAATTCTACTTTATATTCAGATTGATGAGTTGAACGCGTCGATTGCCATAATGGAGCAGCATAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACC                                                                                                                                      <  SRR19973061.3667591/150‑1 (MQ=60)
                 TGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATCCCG                                                                                                                                     >  SRR19973061.1369606/1‑150 (MQ=60)
                 TGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCG                                                                                                                                     <  SRR19973061.3514912/150‑1 (MQ=60)
                 TGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCG                                                                                                                                     >  SRR19973061.1379502/1‑150 (MQ=60)
                 TGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCG                                                                                                                                     <  SRR19973061.3330664/150‑1 (MQ=60)
                  GCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGG                                                                                                                                    >  SRR19973061.1734875/1‑150 (MQ=60)
                   CGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGT                                                                                                                                   >  SRR19973061.1378350/1‑150 (MQ=60)
                      GAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATT                                                                                                                                >  SRR19973061.688875/1‑150 (MQ=60)
                        AACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCA                                                                                                                              <  SRR19973061.2506656/150‑1 (MQ=60)
                        AACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCA                                                                                                                              <  SRR19973061.579145/150‑1 (MQ=60)
                         ACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAG                                                                                                                             <  SRR19973061.1872711/150‑1 (MQ=60)
                           GCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCACAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCT                                                                                                                           >  SRR19973061.3418869/1‑150 (MQ=60)
                           GCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCCTACCGGAATTCAGCT                                                                                                                           >  SRR19973061.3418890/1‑150 (MQ=60)
                           GCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCT                                                                                                                           >  SRR19973061.1839263/1‑150 (MQ=60)
                             AAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGA                                                                                                                         >  SRR19973061.1530687/1‑150 (MQ=60)
                              AAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGAT                                                                                                                        <  SRR19973061.1549829/150‑1 (MQ=60)
                               AACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATT                                                                                                                       >  SRR19973061.2608052/1‑150 (MQ=60)
                               AACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATT                                                                                                                       <  SRR19973061.1470509/150‑1 (MQ=60)
                               AACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATT                                                                                                                       >  SRR19973061.695839/1‑150 (MQ=60)
                                   CGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCA                                                                                                                   >  SRR19973061.550100/1‑150 (MQ=60)
                                   CGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCA                                                                                                                   >  SRR19973061.3243969/1‑150 (MQ=60)
                                    GCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAG                                                                                                                  <  SRR19973061.3606762/150‑1 (MQ=60)
                                       CAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGG                                                                                                               <  SRR19973061.2602106/150‑1 (MQ=60)
                                       CAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGG                                                                                                               <  SRR19973061.898470/150‑1 (MQ=60)
                                       CAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGG                                                                                                               <  SRR19973061.3643451/150‑1 (MQ=60)
                                        AATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGT                                                                                                              >  SRR19973061.2274523/1‑150 (MQ=60)
                                           CAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAA                                                                                                           <  SRR19973061.2795974/150‑1 (MQ=60)
                                            AACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAG                                                                                                          >  SRR19973061.3776935/1‑150 (MQ=60)
                                              CTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAA                                                                                                        >  SRR19973061.2494519/1‑150 (MQ=60)
                                                 AATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGC                                                                                                     >  SRR19973061.2388296/1‑150 (MQ=60)
                                                 AATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGC                                                                                                     >  SRR19973061.2388565/1‑150 (MQ=60)
                                                 AATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGC                                                                                                     >  SRR19973061.2699133/1‑150 (MQ=60)
                                                     AACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGC                                                                                                 <  SRR19973061.1552521/150‑1 (MQ=60)
                                                     AACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGC                                                                                                 >  SRR19973061.1086693/1‑150 (MQ=60)
                                                      ACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCG                                                                                                <  SRR19973061.3783392/150‑1 (MQ=60)
                                                        AGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTG                                                                                              <  SRR19973061.2280302/150‑1 (MQ=60)
                                                          ATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGA                                                                                            >  SRR19973061.1737893/1‑150 (MQ=60)
                                                          ATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGA                                                                                            >  SRR19973061.1736408/1‑150 (MQ=60)
                                                          ATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGA                                                                                            <  SRR19973061.3738238/150‑1 (MQ=60)
                                                           TTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGAC                                                                                           <  SRR19973061.3246802/150‑1 (MQ=60)
                                                            TGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACA                                                                                          <  SRR19973061.991229/150‑1 (MQ=60)
                                                             GATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAG                                                                                         >  SRR19973061.1402463/1‑150 (MQ=60)
                                                                       ACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGG                                                                               <  SRR19973061.2858375/150‑1 (MQ=60)
                                                                              GATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTT                                                                        <  SRR19973061.763597/150‑1 (MQ=60)
                                                                              GATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTT                                                                        >  SRR19973061.1502297/1‑150 (MQ=60)
                                                                              GATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTT                                                                        >  SRR19973061.1502330/1‑150 (MQ=60)
                                                                              GATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATAACGGTATTCAGATGATTCTAAGCGGTGAAGAAATCCAGCGTGGACAGCGTTTACAGGATTATTT                                                                        <  SRR19973061.2518299/150‑1 (MQ=60)
                                                                               ATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTC                                                                       <  SRR19973061.758241/150‑1 (MQ=60)
                                                                                TTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCG                                                                      >  SRR19973061.1416952/1‑150 (MQ=60)
                                                                                    CAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTA                                                                  <  SRR19973061.1256223/150‑1 (MQ=60)
                                                                                        CTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTC                                                              <  SRR19973061.3359349/150‑1 (MQ=60)
                                                                                         TGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCA                                                             >  SRR19973061.917288/1‑150 (MQ=60)
                                                                                           GAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAAC                                                           <  SRR19973061.2507388/150‑1 (MQ=60)
                                                                                            AGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACC                                                          <  SRR19973061.2863917/150‑1 (MQ=60)
                                                                                            AGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACC                                                          <  SRR19973061.3211890/150‑1 (MQ=60)
                                                                                             GCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCA                                                         <  SRR19973061.3307475/150‑1 (MQ=60)
                                                                                             GCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCA                                                         <  SRR19973061.3307491/150‑1 (MQ=60)
                                                                                              CAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAG                                                        <  SRR19973061.663665/150‑1 (MQ=60)
                                                                                              CAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAG                                                        <  SRR19973061.439000/150‑1 (MQ=60)
                                                                                                  AAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGC                                                    >  SRR19973061.1426570/1‑150 (MQ=60)
                                                                                                  AAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGC                                                    <  SRR19973061.1768130/150‑1 (MQ=60)
                                                                                                  AAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGC                                                    >  SRR19973061.2081831/1‑150 (MQ=60)
                                                                                                     AAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGAC                                                 >  SRR19973061.2882290/1‑150 (MQ=60)
                                                                                                        CCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAG                                              <  SRR19973061.3490883/150‑1 (MQ=60)
                                                                                                        CCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAG                                              >  SRR19973061.1574406/1‑150 (MQ=60)
                                                                                                          GCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAA                                            >  SRR19973061.431800/1‑150 (MQ=60)
                                                                                                            CCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAAC                                          >  SRR19973061.3278321/1‑150 (MQ=60)
                                                                                                             CAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACC                                         <  SRR19973061.3791873/150‑1 (MQ=60)
                                                                                                             CAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACC                                         <  SRR19973061.3791929/150‑1 (MQ=60)
                                                                                                             CAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACC                                         <  SRR19973061.3305728/150‑1 (MQ=60)
                                                                                                                GAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATT                                      <  SRR19973061.3693884/150‑1 (MQ=60)
                                                                                                                GAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATT                                      <  SRR19973061.2233212/150‑1 (MQ=60)
                                                                                                                GAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATT                                      <  SRR19973061.2233168/150‑1 (MQ=60)
                                                                                                                GAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATT                                      <  SRR19973061.2233275/150‑1 (MQ=60)
                                                                                                                GAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATT                                      <  SRR19973061.872198/150‑1 (MQ=60)
                                                                                                                GAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATT                                      <  SRR19973061.2240455/150‑1 (MQ=60)
                                                                                                                     CAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCA                                 <  SRR19973061.2307584/150‑1 (MQ=60)
                                                                                                                     CAGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCA                                 <  SRR19973061.1995013/150‑1 (MQ=60)
                                                                                                                      AGCCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCTGGCGCGACAAGAAACCATTGCCCAG                                <  SRR19973061.3691815/150‑1 (MQ=60)
                                                                                                                        CCTCGCCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTT                              >  SRR19973061.3379970/1‑150 (MQ=60)
                                                                                                                             CCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGC                         <  SRR19973061.2802022/150‑1 (MQ=60)
                                                                                                                             CCGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGC                         >  SRR19973061.627622/1‑150 (MQ=60)
                                                                                                                              CGCACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCA                        >  SRR19973061.2903319/1‑150 (MQ=60)
                                                                                                                                CACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAA                      >  SRR19973061.2784518/1‑150 (MQ=60)
                                                                                                                                 ACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAAC                     <  SRR19973061.3001481/150‑1 (MQ=60)
                                                                                                                                 ACAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAAC                     <  SRR19973061.2594951/150‑1 (MQ=60)
                                                                                                                                  CAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACG                    >  SRR19973061.677591/1‑150 (MQ=60)
                                                                                                                                  CAACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACG                    <  SRR19973061.3610844/150‑1 (MQ=60)
                                                                                                                                   AACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGAGTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGC                   <  SRR19973061.2603999/150‑1 (MQ=60)
                                                                                                                                    ACTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCG                  >  SRR19973061.545125/1‑150 (MQ=60)
                                                                                                                                     CTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGT                 <  SRR19973061.3209203/150‑1 (MQ=60)
                                                                                                                                     CTGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGT                 <  SRR19973061.2179872/150‑1 (MQ=60)
                                                                                                                                      TGGATGCCGCATTCAGTCAGGGCGAGCATACCGGTAGTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTG                <  SRR19973061.3694667/150‑1 (MQ=60)
                                                                                                                                       GGATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGA               <  SRR19973061.644358/150‑1 (MQ=60)
                                                                                                                                        GATGCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAA              <  SRR19973061.527990/150‑1 (MQ=60)
                                                                                                                                           GCCGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAA           <  SRR19973061.832188/150‑1 (MQ=60)
                                                                                                                                             CGCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGT         <  SRR19973061.1536579/150‑1 (MQ=60)
                                                                                                                                              GCATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTC        <  SRR19973061.2036163/150‑1 (MQ=60)
                                                                                                                                               CATTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCG       >  SRR19973061.2491840/1‑150 (MQ=60)
                                                                                                                                                 TTCAGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCC     <  SRR19973061.1763329/150‑1 (MQ=60)
                                                                                                                                                    AGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATA  <  SRR19973061.1937618/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATG  >  CP009273/3780425‑3780722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑