Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,783,727 C→A A327S (GCT→TCT)  tdh ← L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,783,7270CA100.0% 673.1 / NA 200A327S (GCT→TCT) tdhL‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (107/93);  total (107/93)

Only 100 of 200 total aligned reads displayed.

AGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAA  >  CP009273/3783584‑3783873
                                                                                                                                               |                                                                                                                                                  
aGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCaat                                                                                                                                              >  2:2906631/1‑149 (MQ=255)
aGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCaaa                                                                                                                                              >  2:2744620/1‑150 (MQ=255)
aGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCaaa                                                                                                                                              <  2:2724266/150‑1 (MQ=255)
   acaATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGcc                                                                                                                                           >  2:3373266/1‑150 (MQ=255)
   acaATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGcc                                                                                                                                           >  1:2843593/1‑150 (MQ=255)
   acaATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGcc                                                                                                                                           <  1:3485760/150‑1 (MQ=255)
    caATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGccc                                                                                                                                          <  2:904196/150‑1 (MQ=255)
     aaTTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCt                                                                                                                                         >  1:2003901/1‑150 (MQ=255)
     aaTTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCt                                                                                                                                         <  1:2504780/150‑1 (MQ=255)
          cGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTgg                                                                                                                                    >  2:731463/1‑150 (MQ=255)
           gAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGa                                                                                                                                   >  2:1639254/1‑150 (MQ=255)
            aaCTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGaa                                                                                                                                  >  1:1886543/1‑150 (MQ=255)
              ctctTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAat                                                                                                                                <  1:2723706/150‑1 (MQ=255)
               tctTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAatc                                                                                                                               <  1:1144424/150‑1 (MQ=255)
               tctTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAatc                                                                                                                               <  2:3474095/150‑1 (MQ=255)
                ctTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAatca                                                                                                                              <  1:2756244/150‑1 (MQ=255)
                     ttaCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGAt                                                                                                                         <  1:519929/150‑1 (MQ=255)
                      taCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATa                                                                                                                        >  1:345603/1‑150 (MQ=255)
                      taCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATa                                                                                                                        >  1:2856715/1‑150 (MQ=255)
                          cTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAgaga                                                                                                                    >  1:3694876/1‑150 (MQ=255)
                           tGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGaa                                                                                                                   >  1:3293082/1‑150 (MQ=255)
                           tGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGaa                                                                                                                   >  1:3291971/1‑150 (MQ=255)
                             aCAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAAc                                                                                                                 <  1:1671330/150‑1 (MQ=255)
                              cAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACg                                                                                                                >  2:982764/1‑150 (MQ=255)
                               aGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGa                                                                                                               >  1:1607417/1‑150 (MQ=255)
                               aGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGa                                                                                                               <  1:2951157/150‑1 (MQ=255)
                               aGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGa                                                                                                               >  1:3717987/1‑150 (MQ=255)
                                 gATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATg                                                                                                             >  2:1264240/1‑150 (MQ=255)
                                 gATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATg                                                                                                             <  2:2088507/150‑1 (MQ=255)
                                     ttACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTa                                                                                                         <  1:1207700/150‑1 (MQ=255)
                                      tACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTaa                                                                                                        <  2:1535190/150‑1 (MQ=255)
                                       aCATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGCTAGAGAAACGATGGGTAAt                                                                                                       >  2:1792234/1‑150 (MQ=255)
                                        cATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATg                                                                                                      <  1:3408747/150‑1 (MQ=255)
                                        cATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATg                                                                                                      <  2:1445848/150‑1 (MQ=255)
                                           atTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGACATCATCGATAGAGAAACGATGGGTAATGATc                                                                                                   >  2:1726052/1‑150 (MQ=255)
                                           atTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATc                                                                                                   <  1:879189/150‑1 (MQ=255)
                                           atTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATc                                                                                                   >  2:9439/1‑150 (MQ=255)
                                           atTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATc                                                                                                   >  2:9429/1‑150 (MQ=255)
                                             ttAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCgg                                                                                                 <  2:3742047/150‑1 (MQ=255)
                                             ttAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCgg                                                                                                 <  1:229474/150‑1 (MQ=255)
                                              tAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGc                                                                                                <  1:867687/150‑1 (MQ=255)
                                               aaCAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCg                                                                                               >  2:1299703/1‑150 (MQ=255)
                                               aaCAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCg                                                                                               <  2:2612220/150‑1 (MQ=255)
                                               aaCAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCg                                                                                               >  1:521566/1‑150 (MQ=255)
                                                    aTTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGa                                                                                          <  1:1765922/150‑1 (MQ=255)
                                                       aTCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCg                                                                                       >  1:1486609/1‑150 (MQ=255)
                                                         ccTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAg                                                                                     <  1:982764/150‑1 (MQ=255)
                                                          cTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAgg                                                                                    <  1:870105/150‑1 (MQ=255)
                                                          cTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAgg                                                                                    >  1:2819096/1‑150 (MQ=255)
                                                          cTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAgg                                                                                    >  1:2968361/1‑150 (MQ=255)
                                                             aGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCa                                                                                 >  2:2032228/1‑150 (MQ=255)
                                                             aGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCa                                                                                 >  2:3712544/1‑150 (MQ=255)
                                                               aTAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGa                                                                               >  2:1659333/1‑150 (MQ=255)
                                                                tAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGAc                                                                              >  1:724063/1‑150 (MQ=255)
                                                                tAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGAc                                                                              >  1:410022/1‑150 (MQ=255)
                                                                 aaaaGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACt                                                                             >  2:2419699/1‑150 (MQ=255)
                                                                 aaaaGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACt                                                                             <  1:1903123/150‑1 (MQ=255)
                                                                     gggCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAAt                                                                         <  1:3523025/150‑1 (MQ=255)
                                                                     gggCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTACCCGGACTGGCCCGAACGCATAGAGTCGAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAAt                                                                         <  1:2608540/150‑1 (MQ=255)
                                                                      ggCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATc                                                                        <  2:1886543/150‑1 (MQ=255)
                                                                        cTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAg                                                                      >  2:206688/1‑150 (MQ=255)
                                                                          ggAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAgcg                                                                    >  2:2921413/1‑150 (MQ=255)
                                                                                 cAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCAt                                                             >  1:3578913/1‑150 (MQ=255)
                                                                                     ccTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTg                                                         >  2:2212934/1‑150 (MQ=255)
                                                                                      cTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGt                                                        >  1:590822/1‑150 (MQ=255)
                                                                                      cTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGt                                                        >  1:2991803/1‑150 (MQ=255)
                                                                                      cTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCAGAACGAATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGt                                                        <  2:2394098/150‑1 (MQ=255)
                                                                                      cTTGTTCGTGTTAAGCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGt                                                        <  2:1299369/150‑1 (MQ=255)
                                                                                       ttGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTa                                                       >  1:1267395/1‑150 (MQ=255)
                                                                                       ttGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTa                                                       >  1:1646574/1‑150 (MQ=255)
                                                                                        tGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTAc                                                      >  2:2716754/1‑150 (MQ=255)
                                                                                         gTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTAcc                                                     <  1:2032649/150‑1 (MQ=255)
                                                                                          ttCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCa                                                    <  2:590883/150‑1 (MQ=255)
                                                                                             gtgtTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGt                                                 <  1:3669465/150‑1 (MQ=255)
                                                                                              tgtTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGtt                                                >  2:3042133/1‑150 (MQ=255)
                                                                                               gtTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGttt                                               <  2:711967/150‑1 (MQ=255)
                                                                                                   aTCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCaaa                                           >  1:1408205/1‑150 (MQ=255)
                                                                                                   aTCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCaaa                                           >  1:3757215/1‑150 (MQ=255)
                                                                                                       cAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACAtc                                       <  1:3373266/150‑1 (MQ=255)
                                                                                                             aGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGa                                 >  1:997965/1‑150 (MQ=255)
                                                                                                              gAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGAc                                >  2:3047265/1‑150 (MQ=255)
                                                                                                                    cTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTaaa                          <  2:3599942/150‑1 (MQ=255)
                                                                                                                        cccGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATAcc                      >  1:2628237/1‑150 (MQ=255)
                                                                                                                        cccGGACTGGCCCGAAAGCATAGAGTCAAAGCCCTTATGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATAcc                      <  1:2338857/150‑1 (MQ=255)
                                                                                                                           ggACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCttt                   <  2:2998210/150‑1 (MQ=255)
                                                                                                                            gACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTa                  <  1:3017296/150‑1 (MQ=255)
                                                                                                                             aCTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTaa                 <  2:2856563/150‑1 (MQ=255)
                                                                                                                             aCTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTaa                 >  1:1918374/1‑150 (MQ=255)
                                                                                                                               tGGCCCGAACGCATAGAGTCAATGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATg               >  2:1077936/1‑150 (MQ=255)
                                                                                                                                   ccGAACGCATAGAGTCAAAGCCATTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACa           <  1:731619/150‑1 (MQ=255)
                                                                                                                                    cGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACaa          >  1:2020460/1‑150 (MQ=255)
                                                                                                                                     gAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAg         >  2:3190514/1‑150 (MQ=255)
                                                                                                                                     gAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAg         >  2:3630161/1‑150 (MQ=255)
                                                                                                                                       aCGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGcc       <  2:2843549/150‑1 (MQ=255)
                                                                                                                                        cGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCt      >  2:3350292/1‑150 (MQ=255)
                                                                                                                                         gCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCtt     <  2:438384/150‑1 (MQ=255)
                                                                                                                                         gCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCtt     <  2:2952127/150‑1 (MQ=255)
                                                                                                                                          cATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCttt    >  1:2464955/1‑150 (MQ=255)
                                                                                                                                           aTAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTa   >  1:1006252/1‑150 (MQ=255)
                                                                                                                                            tAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTaa  >  1:470502/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
AGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAA  >  CP009273/3783584‑3783873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGA  >  CP009273/3783579‑3783876
                                                                                                                                                    |                                                                                                                                                     
AGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAG                                                                                                                                                      >  SRR19973061.1539014/1‑150 (MQ=60)
        ACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCC                                                                                                                                              >  SRR19973061.2850835/1‑150 (MQ=60)
        ACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCC                                                                                                                                              >  SRR19973061.2850879/1‑150 (MQ=60)
        ACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCC                                                                                                                                              <  SRR19973061.3494740/150‑1 (MQ=60)
        ACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCC                                                                                                                                              <  SRR19973061.3494673/150‑1 (MQ=60)
          AATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCT                                                                                                                                            >  SRR19973061.2008922/1‑150 (MQ=60)
          AATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCT                                                                                                                                            <  SRR19973061.2511158/150‑1 (MQ=60)
             TACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCT                                                                                                                                         >  SRR19973061.3005945/1‑150 (MQ=60)
                 AACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAA                                                                                                                                     >  SRR19973061.1891229/1‑150 (MQ=60)
                   CTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAAT                                                                                                                                   <  SRR19973061.2730714/150‑1 (MQ=60)
                    TCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATC                                                                                                                                  <  SRR19973061.1147333/150‑1 (MQ=60)
                     CTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCA                                                                                                                                 <  SRR19973061.2763341/150‑1 (MQ=60)
                          TTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGAT                                                                                                                            <  SRR19973061.521298/150‑1 (MQ=60)
                          TTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGACTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGAT                                                                                                                            <  SRR19973061.979257/150‑1 (MQ=60)
                           TACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATA                                                                                                                           >  SRR19973061.346524/1‑150 (MQ=60)
                           TACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATA                                                                                                                           >  SRR19973061.2864029/1‑150 (MQ=60)
                           TACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATA                                                                                                                           >  SRR19973061.2863877/1‑150 (MQ=60)
                               CTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGA                                                                                                                       >  SRR19973061.3704319/1‑150 (MQ=60)
                               CTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGA                                                                                                                       >  SRR19973061.3704335/1‑150 (MQ=60)
                                TGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAA                                                                                                                      >  SRR19973061.3301561/1‑150 (MQ=60)
                                TGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAA                                                                                                                      >  SRR19973061.3300448/1‑150 (MQ=60)
                                TGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAA                                                                                                                      >  SRR19973061.3300505/1‑150 (MQ=60)
                                 GACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAA                                                                                                                     >  SRR19973061.1651546/1‑150 (MQ=60)
                                  ACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAAC                                                                                                                    <  SRR19973061.1675529/150‑1 (MQ=60)
                                    AGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGA                                                                                                                  <  SRR19973061.2958764/150‑1 (MQ=60)
                                    AGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGA                                                                                                                  >  SRR19973061.1611416/1‑150 (MQ=60)
                                    AGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGA                                                                                                                  >  SRR19973061.3727486/1‑150 (MQ=60)
                                     GGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGAT                                                                                                                 >  SRR19973061.3010720/1‑150 (MQ=60)
                                        TTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGG                                                                                                              <  SRR19973061.258502/150‑1 (MQ=60)
                                          TTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTA                                                                                                            <  SRR19973061.1210731/150‑1 (MQ=60)
                                           TACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGCAATCATCGATAGAGAAACGATGGGTAA                                                                                                           >  SRR19973061.3444832/1‑150 (MQ=60)
                                            ACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAAT                                                                                                          <  SRR19973061.3351239/150‑1 (MQ=60)
                                            ACATATTTAACAGATTATCCTCAGATAAAAGGGATGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACATTCCCGGACAGGCCCGAACGCATAGAATCAAACACCTACAGGAAATCATCGATATATAAACAATAGGTAAT                                                                                                          >  SRR19973061.3386204/1‑150 (MQ=60)
                                             CATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATG                                                                                                         <  SRR19973061.3417501/150‑1 (MQ=60)
                                                ATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATC                                                                                                      <  SRR19973061.1929746/150‑1 (MQ=60)
                                                ATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATC                                                                                                      <  SRR19973061.881470/150‑1 (MQ=60)
                                                 TTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCG                                                                                                     >  SRR19973061.19161/1‑150 (MQ=60)
                                                  TTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGG                                                                                                    <  SRR19973061.230145/150‑1 (MQ=60)
                                                  TTAACAGATTATCCTAAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGG                                                                                                    <  SRR19973061.230765/150‑1 (MQ=60)
                                                   TAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGC                                                                                                   <  SRR19973061.869935/150‑1 (MQ=60)
                                                    AACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCG                                                                                                  >  SRR19973061.522155/1‑150 (MQ=60)
                                                    AACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCG                                                                                                  >  SRR19973061.522943/1‑150 (MQ=60)
                                                    AACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCG                                                                                                  >  SRR19973061.1744366/1‑150 (MQ=60)
                                                         ATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGA                                                                                             <  SRR19973061.1770347/150‑1 (MQ=60)
                                                          TTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGAT                                                                                            <  SRR19973061.2256051/150‑1 (MQ=60)
                                                          TTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGAT                                                                                            >  SRR19973061.1545538/1‑150 (MQ=60)
                                                            ATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCG                                                                                          >  SRR19973061.3639440/1‑150 (MQ=60)
                                                            ATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCG                                                                                          >  SRR19973061.1490338/1‑150 (MQ=60)
                                                              CCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAG                                                                                        <  SRR19973061.985267/150‑1 (MQ=60)
                                                               CTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGG                                                                                       <  SRR19973061.878702/150‑1 (MQ=60)
                                                               CTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGG                                                                                       >  SRR19973061.2826325/1‑150 (MQ=60)
                                                               CTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGG                                                                                       <  SRR19973061.872365/150‑1 (MQ=60)
                                                               CTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGG                                                                                       >  SRR19973061.2975470/1‑150 (MQ=60)
                                                               CTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGG                                                                                       >  SRR19973061.2976017/1‑150 (MQ=60)
                                                                  AGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCA                                                                                    <  SRR19973061.516067/150‑1 (MQ=60)
                                                                  AGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCA                                                                                    >  SRR19973061.409936/1‑150 (MQ=60)
                                                                     TAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGAC                                                                                 >  SRR19973061.725954/1‑150 (MQ=60)
                                                                     TAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGAC                                                                                 >  SRR19973061.411141/1‑150 (MQ=60)
                                                                      AAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACT                                                                                <  SRR19973061.1907839/150‑1 (MQ=60)
                                                                      AAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACT                                                                                >  SRR19973061.2393398/1‑150 (MQ=60)
                                                                          GGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAAT                                                                            <  SRR19973061.3532051/150‑1 (MQ=60)
                                                                          GGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTACCCGGACTGGCCCGAACGCATAGAGTCGAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAAT                                                                            <  SRR19973061.2615244/150‑1 (MQ=60)
                                                                               GGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCG                                                                       >  SRR19973061.1231617/1‑150 (MQ=60)
                                                                                 AATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCC                                                                     <  SRR19973061.761925/150‑1 (MQ=60)
                                                                                  ATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCG                                                                    >  SRR19973061.3694780/1‑150 (MQ=60)
                                                                                  ATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCG                                                                    <  SRR19973061.3211369/150‑1 (MQ=60)
                                                                                      CAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCAT                                                                >  SRR19973061.3588089/1‑150 (MQ=60)
                                                                                      CAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATAATCTGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCAT                                                                >  SRR19973061.2653310/1‑150 (MQ=60)
                                                                                         CCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTT                                                             <  SRR19973061.1899183/150‑1 (MQ=60)
                                                                                         CCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTT                                                             <  SRR19973061.3403302/150‑1 (MQ=60)
                                                                                           CTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGT                                                           >  SRR19973061.592361/1‑150 (MQ=60)
                                                                                           CTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGT                                                           >  SRR19973061.2999527/1‑150 (MQ=60)
                                                                                            TTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTA                                                          >  SRR19973061.1270523/1‑150 (MQ=60)
                                                                                            TTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTA                                                          >  SRR19973061.1650710/1‑150 (MQ=60)
                                                                                              GTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACC                                                        <  SRR19973061.2037331/150‑1 (MQ=60)
                                                                                              GTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACC                                                        <  SRR19973061.2037752/150‑1 (MQ=60)
                                                                                                  GTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGT                                                    <  SRR19973061.3678849/150‑1 (MQ=60)
                                                                                                   TGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTT                                                   >  SRR19973061.439576/1‑150 (MQ=60)
                                                                                                   TGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTT                                                   >  SRR19973061.2810682/1‑150 (MQ=60)
                                                                                                     TTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTC                                                 <  SRR19973061.1201037/150‑1 (MQ=60)
                                                                                                        ATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAA                                              >  SRR19973061.3765584/1‑150 (MQ=60)
                                                                                                        ATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAA                                              >  SRR19973061.3766826/1‑150 (MQ=60)
                                                                                                        ATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAA                                              >  SRR19973061.1411757/1‑150 (MQ=60)
                                                                                                            CAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATC                                          <  SRR19973061.2940220/150‑1 (MQ=60)
                                                                                                            CAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATC                                          <  SRR19973061.3381935/150‑1 (MQ=60)
                                                                                                             AGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCT                                         <  SRR19973061.2751682/150‑1 (MQ=60)
                                                                                                              GCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTC                                        <  SRR19973061.994296/150‑1 (MQ=60)
                                                                                                                 CAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACG                                     <  SRR19973061.3481544/150‑1 (MQ=60)
                                                                                                                  AGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGA                                    >  SRR19973061.1000509/1‑150 (MQ=60)
                                                                                                                  AGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGA                                    >  SRR19973061.1000550/1‑150 (MQ=60)
                                                                                                                  AGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGA                                    >  SRR19973061.1026359/1‑150 (MQ=60)
                                                                                                                     ATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCG                                 >  SRR19973061.1629635/1‑150 (MQ=60)
                                                                                                                       AACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTA                               <  SRR19973061.2472018/150‑1 (MQ=60)
                                                                                                                        ACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAA                              >  SRR19973061.1787153/1‑150 (MQ=60)
                                                                                                                            TCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATAC                          <  SRR19973061.1405578/150‑1 (MQ=60)
                                                                                                                             CCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACC                         >  SRR19973061.2634990/1‑150 (MQ=60)
                                                                                                                             CCCGGACTGGCCCGAAAGCATAGAGTCAAAGCCCTTATGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACC                         <  SRR19973061.2344794/150‑1 (MQ=60)
                                                                                                                              CCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCT                        <  SRR19973061.872206/150‑1 (MQ=60)
                                                                                                                              CCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCT                        >  SRR19973061.653593/1‑150 (MQ=60)
                                                                                                                                 GACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTA                     <  SRR19973061.3025086/150‑1 (MQ=60)
                                                                                                                                  ACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGACAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGATTCAAACATCTCACGACCGTAAATACCTTTAA                    >  SRR19973061.578243/1‑150 (MQ=60)
                                                                                                                                  ACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAA                    >  SRR19973061.1923130/1‑150 (MQ=60)
                                                                                                                                    TGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATG                  <  SRR19973061.1428719/150‑1 (MQ=60)
                                                                                                                                     GGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGA                 <  SRR19973061.2618720/150‑1 (MQ=60)
                                                                                                                                        CCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACA              <  SRR19973061.733379/150‑1 (MQ=60)
                                                                                                                                        CCGAACGCATAGAGTCAAAGCCATTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACA              <  SRR19973061.733537/150‑1 (MQ=60)
                                                                                                                                         CGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAA             >  SRR19973061.2024314/1‑150 (MQ=60)
                                                                                                                                         CGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAA             >  SRR19973061.2025535/1‑150 (MQ=60)
                                                                                                                                         CGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAA             >  SRR19973061.3536559/1‑150 (MQ=60)
                                                                                                                                               CATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTT       >  SRR19973061.2471232/1‑150 (MQ=60)
                                                                                                                                                ATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTA      >  SRR19973061.1008816/1‑150 (MQ=60)
                                                                                                                                                 TAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAA     >  SRR19973061.471772/1‑150 (MQ=60)
                                                                                                                                                   GAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAG   <  SRR19973061.2378217/150‑1 (MQ=60)
                                                                                                                                                    AGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGA  >  SRR19973061.3472073/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
AGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGA  >  CP009273/3783579‑3783876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑