Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 158,287 C→A 100% D78Y (GAC→TAC)  ligT ← 2'‑5' RNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273158,2870CA100.0% 650.7 / ‑6.7 196D78Y (GAC→TAC) ligT2'‑5' RNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (101/94);  minor base T (0/1);  total (101/95)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 201 total aligned reads displayed.

ATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGG  >  CP009273/158142‑158431
                                                                                                                                                 |                                                                                                                                                
atatGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGtaat                                                                                                                                                >  1:652142/1‑148 (MQ=255)
  atGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGtaatca                                                                                                                                              >  2:201549/1‑148 (MQ=255)
    gtgGATGAACCGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAg                                                                                                                                            <  2:1299130/148‑1 (MQ=255)
       gATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGt                                                                                                                                         >  2:111711/1‑148 (MQ=255)
        aTGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTg                                                                                                                                        >  1:1393624/1‑148 (MQ=255)
        aTGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTg                                                                                                                                        >  1:3175688/1‑148 (MQ=255)
         tGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGa                                                                                                                                       >  2:2601724/1‑148 (MQ=255)
         tGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGa                                                                                                                                       >  2:2601599/1‑148 (MQ=255)
           aaaCGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAgt                                                                                                                                     <  1:3063749/148‑1 (MQ=255)
           aaaCGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAgt                                                                                                                                     >  2:3474669/1‑148 (MQ=255)
           aaaCGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGCCCGGCGTAATCAAGCGTGAgt                                                                                                                                     <  1:2910333/148‑1 (MQ=255)
                gACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGaa                                                                                                                                >  1:2023987/1‑148 (MQ=255)
                    aTTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCa                                                                                                                            >  2:22348/1‑148 (MQ=255)
                     ttGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCGAGCGTGAGTGTGACACCAg                                                                                                                           >  2:3428861/1‑148 (MQ=255)
                     ttGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAg                                                                                                                           >  2:2585317/1‑148 (MQ=255)
                     ttGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAg                                                                                                                           >  1:2468920/1‑148 (MQ=255)
                     ttGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAg                                                                                                                           >  2:1480528/1‑148 (MQ=255)
                      tGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAgg                                                                                                                          >  2:2536338/1‑148 (MQ=255)
                       gCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGt                                                                                                                         <  1:1817029/148‑1 (MQ=255)
                       gCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGt                                                                                                                         <  1:923945/148‑1 (MQ=255)
                       gCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGt                                                                                                                         <  1:1532797/148‑1 (MQ=255)
                        cTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGtt                                                                                                                        >  1:54256/1‑148 (MQ=255)
                           tGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGAc                                                                                                                     >  1:579515/1‑148 (MQ=255)
                                acaacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtc                                                                                                                >  2:916850/1‑148 (MQ=255)
                                acaacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtc                                                                                                                <  1:2985710/148‑1 (MQ=255)
                                 caacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtcc                                                                                                               >  1:2031893/1‑148 (MQ=255)
                                   acCGCTGCGGGCAGACTGTGAACTGAGCATATTCGACAGCTGTATTAAGCCGCGTGGCGGCTGACGCATCACTAACCACACCAGACGCGAACGCAGCCATTGTCCGGCGTTATCAATCGTGATTGTGAAACCAGTTTGACGAAtccgt                                                                                                             <  2:2384718/148‑1 (MQ=255)
                                    ccGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgtc                                                                                                            <  2:2031141/148‑1 (MQ=255)
                                    ccGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgtc                                                                                                            <  1:285970/148‑1 (MQ=255)
                                    ccGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgtc                                                                                                            <  1:679145/148‑1 (MQ=255)
                                     cGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgtcc                                                                                                           >  1:164396/1‑148 (MQ=255)
                                         gCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCt                                                                                                       >  1:2286580/1‑148 (MQ=255)
                                          cGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTa                                                                                                      >  2:1663088/1‑148 (MQ=255)
                                          cGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTa                                                                                                      <  2:3259499/148‑1 (MQ=255)
                                            ggCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTaaa                                                                                                    <  2:3175688/148‑1 (MQ=255)
                                             gCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTaaaa                                                                                                   <  1:324010/148‑1 (MQ=255)
                                             gCAGCCTGTGAACGGAGCATAGTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTaaaa                                                                                                   <  1:1243712/148‑1 (MQ=255)
                                              cAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAag                                                                                                  >  2:311375/1‑148 (MQ=255)
                                              cAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAAACAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAag                                                                                                  >  2:111044/1‑148 (MQ=255)
                                                gCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAagag                                                                                                <  2:1494023/148‑1 (MQ=255)
                                                gCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAagag                                                                                                <  2:1493978/148‑1 (MQ=255)
                                                  cTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGaa                                                                                              <  1:1330816/148‑1 (MQ=255)
                                                       aaCGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAgcgc                                                                                         <  1:2342845/148‑1 (MQ=255)
                                                        aCGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGcc                                                                                        >  1:3231783/1‑148 (MQ=255)
                                                         cGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCt                                                                                       <  2:2975889/148‑1 (MQ=255)
                                                         cGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCt                                                                                       <  2:530690/148‑1 (MQ=255)
                                                          ggAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCtt                                                                                      <  1:79418/148‑1 (MQ=255)
                                                          ggAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCtt                                                                                      <  2:313072/148‑1 (MQ=255)
                                                           gAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTtc                                                                                     <  1:3485117/148‑1 (MQ=255)
                                                             gCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTtctc                                                                                   <  1:2011369/148‑1 (MQ=255)
                                                              cATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTctct                                                                                  >  2:1742982/1‑148 (MQ=255)
                                                              cATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTctct                                                                                  <  2:1567285/148‑1 (MQ=255)
                                                                tatTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCttt                                                                                <  2:2072050/148‑1 (MQ=255)
                                                                 atTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtc                                                                               <  2:2006119/148‑1 (MQ=255)
                                                                 atTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtc                                                                               >  2:1864954/1‑148 (MQ=255)
                                                                 atTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtc                                                                               <  1:2759772/148‑1 (MQ=255)
                                                                 atTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtc                                                                               >  1:817081/1‑148 (MQ=255)
                                                                  ttCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtct                                                                              <  2:1170746/148‑1 (MQ=255)
                                                                   tCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtctc                                                                             >  2:2141230/1‑148 (MQ=255)
                                                                     gCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCgg                                                                           >  1:1125104/1‑148 (MQ=255)
                                                                     gCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCgg                                                                           <  1:1857606/148‑1 (MQ=255)
                                                                       cAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGgcg                                                                         <  2:2381131/148‑1 (MQ=255)
                                                                        aGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGgcgc                                                                        <  2:87854/148‑1 (MQ=255)
                                                                         gCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCt                                                                       >  2:750801/1‑148 (MQ=255)
                                                                            ggATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCAc                                                                    <  1:393231/148‑1 (MQ=255)
                                                                                tAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCg                                                                >  2:2447031/1‑148 (MQ=255)
                                                                                 aaGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGc                                                               <  1:602000/148‑1 (MQ=255)
                                                                                  aGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGcc                                                              >  2:663830/1‑148 (MQ=255)
                                                                                   gCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCt                                                             >  2:412650/1‑148 (MQ=255)
                                                                                              gcTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCag                                                  >  1:598327/1‑148 (MQ=255)
                                                                                               cTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGACTCCGTCCGGCTAAAAGAGAAAGCGCCGTCTCTGTCTCGGCGCTCACTTCGCCTAAAAATGCCaga                                                 >  1:3254617/1‑148 (MQ=255)
                                                                                                 gACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGt                                               <  2:329443/148‑1 (MQ=255)
                                                                                                  aCGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTc                                              <  2:2286580/148‑1 (MQ=255)
                                                                                                     cATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGa                                           <  2:2468920/148‑1 (MQ=255)
                                                                                                        cccTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGc                                        >  2:1965733/1‑148 (MQ=255)
                                                                                                         ccTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCa                                       >  2:1804442/1‑148 (MQ=255)
                                                                                                          cTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCaa                                      <  2:3085736/148‑1 (MQ=255)
                                                                                                            caccacaccacACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAAt                                    <  1:386579/147‑1 (MQ=255)
                                                                                                            aaCCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAAt                                    <  1:45342/148‑1 (MQ=255)
                                                                                                            aaCCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAAt                                    <  1:2863359/148‑1 (MQ=255)
                                                                                                             accacaccacACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAAtt                                   >  2:1891821/1‑148 (MQ=255)
                                                                                                              ccacaccacaCGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTa                                  >  1:534976/1‑148 (MQ=255)
                                                                                                              ccacaccacaCGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTa                                  >  2:2634961/1‑148 (MQ=255)
                                                                                                               cacaccacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTAt                                 <  1:1023927/148‑1 (MQ=255)
                                                                                                               cacaccacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTAt                                 >  1:3428800/1‑148 (MQ=255)
                                                                                                                   ccacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATcggc                             >  2:1354344/1‑148 (MQ=255)
                                                                                                                       acGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGgcggcg                         <  1:3126401/148‑1 (MQ=255)
                                                                                                                            aaCGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCgg                    >  2:3106392/1‑148 (MQ=255)
                                                                                                                            aaCGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCgg                    <  1:2178680/148‑1 (MQ=255)
                                                                                                                            aaCGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCgg                    <  2:2931359/148‑1 (MQ=255)
                                                                                                                                     aTTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGc           <  1:3224183/148‑1 (MQ=255)
                                                                                                                                     aTTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGc           <  1:1480528/148‑1 (MQ=255)
                                                                                                                                        gTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTc        <  2:2221665/148‑1 (MQ=255)
                                                                                                                                          ccGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAg      <  2:64786/148‑1 (MQ=255)
                                                                                                                                           cGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCACATTATCGGCGGCGACCGGACGTCCCGCCTCAgg     >  1:1152358/1‑148 (MQ=255)
                                                                                                                                            ggCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGt    <  2:3374862/148‑1 (MQ=255)
                                                                                                                                            ggCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGt    <  2:2773423/148‑1 (MQ=255)
                                                                                                                                            ggCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGt    <  2:1390558/148‑1 (MQ=255)
                                                                                                                                             gCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTg   <  1:2296433/148‑1 (MQ=255)
                                                                                                                                              cGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTgg  >  2:3343466/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGG  >  CP009273/158142‑158431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGAAGT  >  CP009273/158138‑158436
                                                                                                                                                     |                                                                                                                                                     
GGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTA                                                                                                                                                       >  SRR19973065.2783154/1‑150 (MQ=60)
 GTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAA                                                                                                                                                      >  SRR19973065.472354/1‑150 (MQ=60)
 GTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAA                                                                                                                                                      >  SRR19973065.3232204/1‑150 (MQ=60)
 GTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAA                                                                                                                                                      >  SRR19973065.783550/1‑150 (MQ=60)
  TAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAAT                                                                                                                                                     <  SRR19973065.2140546/150‑1 (MQ=60)
  TAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAAT                                                                                                                                                     >  SRR19973065.656436/1‑150 (MQ=60)
  TAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCGGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAAT                                                                                                                                                     <  SRR19973065.1212427/150‑1 (MQ=60)
   AATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATC                                                                                                                                                    >  SRR19973065.672234/1‑150 (MQ=60)
          GGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTG                                                                                                                                             >  SRR19973065.3194887/1‑150 (MQ=60)
          GGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTG                                                                                                                                             >  SRR19973065.1402770/1‑150 (MQ=60)
          GGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTG                                                                                                                                             >  SRR19973065.2339213/1‑150 (MQ=60)
               AAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGT                                                                                                                                        <  SRR19973065.3082483/150‑1 (MQ=60)
               AAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGCCCGGCGTAATCAAGCGTGAGTGT                                                                                                                                        <  SRR19973065.2928051/150‑1 (MQ=60)
                  CGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAA                                                                                                                                     >  SRR19973065.2037001/1‑150 (MQ=60)
                  CGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAA                                                                                                                                     >  SRR19973065.2036978/1‑150 (MQ=60)
                  CGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAA                                                                                                                                     >  SRR19973065.3256670/1‑150 (MQ=60)
                   GGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAA                                                                                                                                    >  SRR19973065.3158217/1‑150 (MQ=60)
                       GATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAG                                                                                                                                >  SRR19973065.2484470/1‑150 (MQ=60)
                       GATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAG                                                                                                                                >  SRR19973065.2484407/1‑150 (MQ=60)
                        ATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGG                                                                                                                               >  SRR19973065.2578828/1‑150 (MQ=60)
                        ATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGG                                                                                                                               >  SRR19973065.1655133/1‑150 (MQ=60)
                        ATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGG                                                                                                                               <  SRR19973065.548014/150‑1 (MQ=60)
                          TGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTT                                                                                                                             >  SRR19973065.54847/1‑150 (MQ=60)
                          TGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTT                                                                                                                             >  SRR19973065.54352/1‑150 (MQ=60)
                           GCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTG                                                                                                                            <  SRR19973065.602264/150‑1 (MQ=60)
                           GCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTG                                                                                                                            <  SRR19973065.930006/150‑1 (MQ=60)
                           GCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTG                                                                                                                            <  SRR19973065.1542899/150‑1 (MQ=60)
                           GCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTG                                                                                                                            <  SRR19973065.1828997/150‑1 (MQ=60)
                             TTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGAC                                                                                                                          >  SRR19973065.583141/1‑150 (MQ=60)
                                 AAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAT                                                                                                                      >  SRR19973065.411869/1‑150 (MQ=60)
                                   AACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCC                                                                                                                    >  SRR19973065.2044934/1‑150 (MQ=60)
                                   AACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCC                                                                                                                    >  SRR19973065.2044176/1‑150 (MQ=60)
                                    ACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCG                                                                                                                   <  SRR19973065.3003986/150‑1 (MQ=60)
                                       ACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCC                                                                                                                >  SRR19973065.165742/1‑150 (MQ=60)
                                        CCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCG                                                                                                               <  SRR19973065.683668/150‑1 (MQ=60)
                                        CCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCG                                                                                                               <  SRR19973065.2204345/150‑1 (MQ=60)
                                        CCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCG                                                                                                               <  SRR19973065.287851/150‑1 (MQ=60)
                                           CTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCT                                                                                                            >  SRR19973065.2300993/1‑150 (MQ=60)
                                                 GCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGA                                                                                                      <  SRR19973065.1251892/150‑1 (MQ=60)
                                                 GCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGA                                                                                                      <  SRR19973065.326261/150‑1 (MQ=60)
                                                 GCAGCCTGTGAACGGAGCATAGTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGA                                                                                                      <  SRR19973065.1251893/150‑1 (MQ=60)
                                                      CTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAG                                                                                                 <  SRR19973065.1339616/150‑1 (MQ=60)
                                                      CTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAG                                                                                                 >  SRR19973065.290635/1‑150 (MQ=60)
                                                       TGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGC                                                                                                <  SRR19973065.1452440/150‑1 (MQ=60)
                                                          GAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCC                                                                                             >  SRR19973065.3251207/1‑150 (MQ=60)
                                                          GAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCC                                                                                             >  SRR19973065.3250889/1‑150 (MQ=60)
                                                           AACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCT                                                                                            <  SRR19973065.2357467/150‑1 (MQ=60)
                                                            ACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTTAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTT                                                                                           <  SRR19973065.1272962/150‑1 (MQ=60)
                                                              GGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCT                                                                                         <  SRR19973065.80218/150‑1 (MQ=60)
                                                               GAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTC                                                                                        <  SRR19973065.3506318/150‑1 (MQ=60)
                                                               GAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTC                                                                                        <  SRR19973065.3506337/150‑1 (MQ=60)
                                                                 GCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTT                                                                                      <  SRR19973065.2024343/150‑1 (MQ=60)
                                                                  CATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTT                                                                                     >  SRR19973065.1383516/1‑150 (MQ=60)
                                                                   ATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTC                                                                                    >  SRR19973065.822454/1‑150 (MQ=60)
                                                                   ATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTC                                                                                    <  SRR19973065.769106/150‑1 (MQ=60)
                                                                   ATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAACCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTC                                                                                    >  SRR19973065.823210/1‑150 (MQ=60)
                                                                    TATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCT                                                                                   >  SRR19973065.1539790/1‑150 (MQ=60)
                                                                     ATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTC                                                                                  <  SRR19973065.2776930/150‑1 (MQ=60)
                                                                       TCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGG                                                                                >  SRR19973065.1132427/1‑150 (MQ=60)
                                                                         GCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCG                                                                              >  SRR19973065.3395502/1‑150 (MQ=60)
                                                                         GCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCG                                                                              <  SRR19973065.1869826/150‑1 (MQ=60)
                                                                         GCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCG                                                                              >  SRR19973065.2790647/1‑150 (MQ=60)
                                                                               TGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACT                                                                        >  SRR19973065.3046504/1‑150 (MQ=60)
                                                                               TGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACT                                                                        >  SRR19973065.1596114/1‑150 (MQ=60)
                                                                                GGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTT                                                                       <  SRR19973065.395975/150‑1 (MQ=60)
                                                                                GGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTT                                                                       <  SRR19973065.395734/150‑1 (MQ=60)
                                                                                 GATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTC                                                                      >  SRR19973065.634054/1‑150 (MQ=60)
                                                                                     AAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCT                                                                  >  SRR19973065.3083598/1‑150 (MQ=60)
                                                                                     AAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCT                                                                  <  SRR19973065.605776/150‑1 (MQ=60)
                                                                                      AGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTA                                                                 <  SRR19973065.347235/150‑1 (MQ=60)
                                                                                                CGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAG                                                       >  SRR19973065.602058/1‑150 (MQ=60)
                                                                                                 GGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGACTCCGTCCGGCTAAAAGAGAAAGCGCCGTCTCTGTCTCGGCGCTCACTTCGCCTAAAAATGCCAGA                                                      >  SRR19973065.3274296/1‑150 (MQ=60)
                                                                                                 GGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGA                                                      <  SRR19973065.523731/150‑1 (MQ=60)
                                                                                                         CATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATG                                              >  SRR19973065.2879718/1‑150 (MQ=60)
                                                                                                         CATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATG                                              >  SRR19973065.1992507/1‑150 (MQ=60)
                                                                                                             CCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAA                                          >  SRR19973065.2949147/1‑150 (MQ=60)
                                                                                                             CCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAA                                          <  SRR19973065.2082066/150‑1 (MQ=60)
                                                                                                                CACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTA                                       <  SRR19973065.389276/150‑1 (MQ=60)
                                                                                                                AACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTA                                       <  SRR19973065.591200/150‑1 (MQ=60)
                                                                                                                AACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTA                                       >  SRR19973065.966506/1‑150 (MQ=60)
                                                                                                                AACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTA                                       <  SRR19973065.45873/150‑1 (MQ=60)
                                                                                                                AACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTA                                       >  SRR19973065.3272718/1‑150 (MQ=60)
                                                                                                                AACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTA                                       >  SRR19973065.538464/1‑150 (MQ=60)
                                                                                                                AACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTA                                       <  SRR19973065.2880933/150‑1 (MQ=60)
                                                                                                                 ACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTAT                                      >  SRR19973065.3449722/1‑150 (MQ=60)
                                                                                                                   CACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCG                                    <  SRR19973065.1030706/150‑1 (MQ=60)
                                                                                                                   CACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCG                                    <  SRR19973065.1030767/150‑1 (MQ=60)
                                                                                                                           ACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGAC                            <  SRR19973065.3145435/150‑1 (MQ=60)
                                                                                                                                AACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGAC                       <  SRR19973065.2192651/150‑1 (MQ=60)
                                                                                                                                    CAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCC                   <  SRR19973065.3225915/150‑1 (MQ=60)
                                                                                                                                       CCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGC                >  SRR19973065.1842380/1‑150 (MQ=60)
                                                                                                                                         ATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCT              <  SRR19973065.2301162/150‑1 (MQ=60)
                                                                                                                                         ATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCT              <  SRR19973065.1489987/150‑1 (MQ=60)
                                                                                                                                         ATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCT              <  SRR19973065.804601/150‑1 (MQ=60)
                                                                                                                                         ATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCT              <  SRR19973065.3243546/150‑1 (MQ=60)
                                                                                                                                             TCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCACATTATCGGCGGCGACCGGACGTCCCGCCTCAGG          >  SRR19973065.1159772/1‑150 (MQ=60)
                                                                                                                                             TCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGG          >  SRR19973065.1159685/1‑150 (MQ=60)
                                                                                                                                                 GCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGG      <  SRR19973065.2310894/150‑1 (MQ=60)
                                                                                                                                                  CGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGA     >  SRR19973065.276606/1‑150 (MQ=60)
                                                                                                                                                    TAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCGCGCCTCAGGTGGGAAG   <  SRR19973065.2301300/150‑1 (MQ=60)
                                                                                                                                                     AATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGAAGT  >  SRR19973065.2480973/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGAAGT  >  CP009273/158138‑158436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑