Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 159,666 G→T 100% G359C (GGT→TGT)  hrpB → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273159,6660GT98.6% 570.5 / ‑1.6 183G359C (GGT→TGT) hrpBputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (102/77);  total (105/78)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 183 total aligned reads displayed.

ACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCG  >  CP009273/159521‑159809
                                                                                                                                                 |                                                                                                                                               
 cgcgTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGtt                                                                                                                                              >  2:2873095/1‑148 (MQ=255)
 cgcgTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGtt                                                                                                                                              >  2:2873001/1‑148 (MQ=255)
 cgcgTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGtt                                                                                                                                              <  1:1672795/148‑1 (MQ=255)
  gcgTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGttt                                                                                                                                             >  1:2990404/1‑148 (MQ=255)
   cgTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTg                                                                                                                                            <  1:2856869/148‑1 (MQ=255)
   cgTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTg                                                                                                                                            <  1:171238/148‑1 (MQ=255)
   cgTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTg                                                                                                                                            <  1:3265107/148‑1 (MQ=255)
      tAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTg                                                                                                                                         >  1:2944002/1‑148 (MQ=255)
        gCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGAt                                                                                                                                       >  2:632691/1‑148 (MQ=255)
            ggCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGaa                                                                                                                                   <  1:534841/148‑1 (MQ=255)
             gCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAAt                                                                                                                                  <  2:2496296/148‑1 (MQ=255)
             gCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAAt                                                                                                                                  >  1:845284/1‑148 (MQ=255)
                 ccATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACt                                                                                                                              >  2:1633039/1‑148 (MQ=255)
                 ccATGACGCAGCGTGCCGGGCGAGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACt                                                                                                                              >  2:2702393/1‑148 (MQ=255)
                   aTGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGTTGGAATTACTGc                                                                                                                            >  1:793023/1‑148 (MQ=255)
                     gACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCaa                                                                                                                          <  2:2176425/148‑1 (MQ=255)
                       cGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATg                                                                                                                        <  2:2093181/148‑1 (MQ=255)
                        gCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATgg                                                                                                                       <  1:2051796/148‑1 (MQ=255)
                         cAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATggg                                                                                                                      >  1:2126880/1‑148 (MQ=255)
                         cAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATggg                                                                                                                      <  1:2324026/148‑1 (MQ=255)
                          aGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATgggg                                                                                                                     <  2:2081467/148‑1 (MQ=255)
                           gCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGa                                                                                                                    <  2:3273009/148‑1 (MQ=255)
                           gCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGa                                                                                                                    >  1:1434614/1‑148 (MQ=255)
                             gTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATg                                                                                                                  <  2:186873/148‑1 (MQ=255)
                              tGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGc                                                                                                                 >  1:1390553/1‑148 (MQ=255)
                                   ggCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGa                                                                                                            >  2:472779/1‑148 (MQ=255)
                                   ggCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGa                                                                                                            >  2:472795/1‑148 (MQ=255)
                                    gcgcgcCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGAt                                                                                                           >  1:1572381/1‑148 (MQ=255)
                                                tCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGACCGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATg                                                                                               <  2:729741/148‑1 (MQ=255)
                                                tCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATg                                                                                               <  1:1852822/148‑1 (MQ=255)
                                                tCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATg                                                                                               <  1:1852843/148‑1 (MQ=255)
                                                tCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATg                                                                                               <  2:3208226/148‑1 (MQ=255)
                                                 cTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATgg                                                                                                                       <  2:267633/123‑1 (MQ=255)
                                                 cTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATgg                                                                                                                       >  1:267633/1‑123 (MQ=255)
                                                    gAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAgct                                                                                           <  2:2956337/148‑1 (MQ=255)
                                                    gAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAgct                                                                                           >  2:1674149/1‑148 (MQ=255)
                                                       ccGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAgctggc                                                                                        <  1:1132729/148‑1 (MQ=255)
                                                         gggTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAgctggctg                                                                                      <  2:1641544/148‑1 (MQ=255)
                                                           gTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGa                                                                                    >  2:3368153/1‑148 (MQ=255)
                                                            tATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGAt                                                                                   <  1:3400359/148‑1 (MQ=255)
                                                            tATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGAt                                                                                   <  2:2219962/148‑1 (MQ=255)
                                                             aTCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATc                                                                                  >  2:3508565/1‑148 (MQ=255)
                                                              tCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCa                                                                                 >  2:1943469/1‑148 (MQ=255)
                                                               cAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCaa                                                                                >  2:494773/1‑148 (MQ=255)
                                                               cAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCaa                                                                                >  1:969994/1‑148 (MQ=255)
                                                                  ccTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCg                                                                             >  2:353399/1‑148 (MQ=255)
                                                                  ccTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCg                                                                             >  2:2856269/1‑148 (MQ=255)
                                                                  ccTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCg                                                                             >  1:3331581/1‑148 (MQ=255)
                                                                  ccTGCATTTAAGCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCg                                                                             <  2:1839581/148‑1 (MQ=255)
                                                                    tGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGcc                                                                           >  2:886684/1‑148 (MQ=255)
                                                                     gCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCa                                                                          <  1:2460167/148‑1 (MQ=255)
                                                                     gCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCa                                                                          <  1:3026623/148‑1 (MQ=255)
                                                                       aTTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCagt                                                                        >  2:1391629/1‑148 (MQ=255)
                                                                        tttAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCagta                                                                       <  1:556452/148‑1 (MQ=255)
                                                                          tAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCagtagt                                                                     <  1:1900129/148‑1 (MQ=255)
                                                                           aaTCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTg                                                                    <  1:1331126/148‑1 (MQ=255)
                                                                            aTCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGa                                                                   <  1:2960109/148‑1 (MQ=255)
                                                                              agcaaaaGAAAAATCATAACTAGCCGCTGCGCAAAGTGAACCTCATATCATAAAAATCGATCTTACCTCTTTGCTTAAGGAATTACTGCAATGGGGATGCCGAGATCCGGCGCAGATGAGCTGTATGGATAAACAGCCAGTACTGaag                                                                 >  2:1183221/5‑147 (MQ=255)
                                                                                     gAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTgg                                                          >  2:2538500/1‑148 (MQ=255)
                                                                                      aaCAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGc                                                         >  1:2525960/1‑148 (MQ=255)
                                                                                       aCAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGcc                                                        >  2:757822/1‑148 (MQ=255)
                                                                                        cAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCACGGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCcg                                                       >  1:1936473/1‑148 (MQ=255)
                                                                                        cAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCcg                                                       >  2:1411011/1‑148 (MQ=255)
                                                                                          aGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCcgcg                                                     >  1:1124601/1‑148 (MQ=255)
                                                                                           gCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGa                                                    >  2:2196640/1‑148 (MQ=255)
                                                                                           gCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGa                                                    >  1:529409/1‑148 (MQ=255)
                                                                                           gCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGa                                                    >  2:1084534/1‑148 (MQ=255)
                                                                                            cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGaa                                                   <  2:2759579/148‑1 (MQ=255)
                                                                                            cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGaa                                                   <  2:1226054/148‑1 (MQ=255)
                                                                                            cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGaa                                                   <  1:1192255/148‑1 (MQ=255)
                                                                                              gAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAAc                                                 >  2:1824895/1‑148 (MQ=255)
                                                                                                aCGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGt                                               <  1:1355349/148‑1 (MQ=255)
                                                                                                aCGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGt                                               <  1:2322400/148‑1 (MQ=255)
                                                                                                aCGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGt                                               <  1:2322615/148‑1 (MQ=255)
                                                                                                   cgccgcGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTg                                            >  2:2782738/1‑148 (MQ=255)
                                                                                                          gcgcAAAGTGACCCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACaaa                                     <  1:1230013/148‑1 (MQ=255)
                                                                                                          gcgcAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACaaa                                     >  1:2141903/1‑148 (MQ=255)
                                                                                                          gcgcAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACaaa                                     >  1:2141662/1‑148 (MQ=255)
                                                                                                          gcgcAAAGTGAACCGGAGATCTTACAAAGCGATCCTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACaaa                                     <  1:350046/148‑1 (MQ=255)
                                                                                                           cgcAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAAt                                    >  1:1723179/1‑148 (MQ=255)
                                                                                                                    aaCCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGc                           >  1:2153735/1‑148 (MQ=255)
                                                                                                                    aaCCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGc                           >  1:77788/1‑148 (MQ=255)
                                                                                                                    aaCCGGAGATCGTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGc                           <  2:1255646/148‑1 (MQ=255)
                                                                                                                     aCCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCa                          >  1:724101/1‑148 (MQ=255)
                                                                                                                      ccGGAGATCTTACAAAGCGATCTTTCCTGTGGGCGGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCGGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCAc                         <  2:1113378/148‑1 (MQ=255)
                                                                                                                          agaTCTTACAAAGCGCTCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGa                     <  2:3123281/148‑1 (MQ=255)
                                                                                                                          agaTCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGa                     >  2:2123702/1‑148 (MQ=255)
                                                                                                                            aTCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAgg                   >  1:1581680/1‑148 (MQ=255)
                                                                                                                            aTCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAgg                   >  1:1428758/1‑148 (MQ=255)
                                                                                                                             tCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAggg                  >  2:2687903/1‑148 (MQ=255)
                                                                                                                              cTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGt                 >  2:1850264/1‑148 (MQ=255)
                                                                                                                              cTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGt                 >  2:3414565/1‑148 (MQ=255)
                                                                                                                              cTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGt                 >  1:1605564/1‑148 (MQ=255)
                                                                                                                              cTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGt                 >  2:1850275/1‑148 (MQ=255)
                                                                                                                                       cGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCtt        >  2:544158/1‑148 (MQ=255)
                                                                                                                                          tCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGt     >  2:1625274/1‑148 (MQ=255)
                                                                                                                                           cTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTg    >  1:222983/1‑148 (MQ=255)
                                                                                                                                             ttCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTgcg  >  1:1295152/1‑148 (MQ=255)
                                                                                                                                             ttCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTgcg  >  1:1293910/1‑148 (MQ=255)
                                                                                                                                             ttCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTgcg  >  1:1907102/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCG  >  CP009273/159521‑159809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCGCAAGGG  >  CP009273/159518‑159815
                                                                                                                                                    |                                                                                                                                                     
TCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTG                                                                                                                                                      <  SRR19973065.3429370/150‑1 (MQ=60)
TCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTG                                                                                                                                                      <  SRR19973065.3428066/150‑1 (MQ=60)
 CAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGT                                                                                                                                                     >  SRR19973065.1563108/1‑150 (MQ=60)
  AACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTT                                                                                                                                                    >  SRR19973065.774348/1‑150 (MQ=60)
   ACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTT                                                                                                                                                   >  SRR19973065.3008710/1‑150 (MQ=60)
    CGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTG                                                                                                                                                  <  SRR19973065.1683982/150‑1 (MQ=60)
      CGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCT                                                                                                                                                <  SRR19973065.172609/150‑1 (MQ=60)
      CGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCT                                                                                                                                                <  SRR19973065.2874420/150‑1 (MQ=60)
      CGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCT                                                                                                                                                <  SRR19973065.3284905/150‑1 (MQ=60)
       GTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTG                                                                                                                                               >  SRR19973065.2961887/1‑150 (MQ=60)
         TAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGAT                                                                                                                                             <  SRR19973065.1730084/150‑1 (MQ=60)
              AGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAAT                                                                                                                                        >  SRR19973065.850770/1‑150 (MQ=60)
               GGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATT                                                                                                                                       <  SRR19973065.538328/150‑1 (MQ=60)
               GGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATT                                                                                                                                       >  SRR19973065.2626496/1‑150 (MQ=60)
                    CCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGTTGGAATTACTGC                                                                                                                                  >  SRR19973065.798272/1‑150 (MQ=60)
                       TGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAAT                                                                                                                               <  SRR19973065.2155462/150‑1 (MQ=60)
                       TGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAATTACTGCAAT                                                                                                                               >  SRR19973065.2100544/1‑150 (MQ=60)
                          CGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGG                                                                                                                            >  SRR19973065.2140566/1‑150 (MQ=60)
                          CGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATATTTCCTGTTTGCTGATGGAATTACTGCAATGGG                                                                                                                            >  SRR19973065.701023/1‑150 (MQ=60)
                           GCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGG                                                                                                                           <  SRR19973065.2064900/150‑1 (MQ=60)
                            CAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGA                                                                                                                          <  SRR19973065.2338578/150‑1 (MQ=60)
                            CAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGA                                                                                                                          >  SRR19973065.1443933/1‑150 (MQ=60)
                               CGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCCGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCGGATGGAATTACTGCAATGGGGATGC                                                                                                                       >  SRR19973065.1638646/1‑150 (MQ=60)
                               CGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGC                                                                                                                       >  SRR19973065.3388687/1‑150 (MQ=60)
                               CGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGC                                                                                                                       >  SRR19973065.1399949/1‑150 (MQ=60)
                               CGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGC                                                                                                                       >  SRR19973065.1399685/1‑150 (MQ=60)
                                   CCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCG                                                                                                                   <  SRR19973065.1297798/150‑1 (MQ=60)
                                   CCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCG                                                                                                                   <  SRR19973065.3295958/150‑1 (MQ=60)
                                     GGGCGCGCCGGGCGTCTGGAGCCGGTTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGAT                                                                                                                 <  SRR19973065.121549/150‑1 (MQ=60)
                                     GGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGAT                                                                                                                 >  SRR19973065.1582847/1‑150 (MQ=60)
                                                GCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGAT                                                                                                      <  SRR19973065.2186896/150‑1 (MQ=60)
                                                  GTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGAgatcggaagagcacacgtctga                                                                                                    >  SRR19973065.269455/1‑128 (MQ=60)
                                                  GTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGA                                                                                                    <  SRR19973065.486446/150‑1 (MQ=60)
                                                   TCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAG                                                                                                   <  SRR19973065.2763125/150‑1 (MQ=60)
                                                   TCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAG                                                                                                   <  SRR19973065.1865025/150‑1 (MQ=60)
                                                   TCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAG                                                                                                   <  SRR19973065.1865046/150‑1 (MQ=60)
                                                       GAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGG                                                                                               <  SRR19973065.3211056/150‑1 (MQ=60)
                                                          CCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTG                                                                                            <  SRR19973065.1140079/150‑1 (MQ=60)
                                                               TATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCA                                                                                       <  SRR19973065.3421143/150‑1 (MQ=60)
                                                                ATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAA                                                                                      >  SRR19973065.976433/1‑150 (MQ=60)
                                                                   AGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCG                                                                                   >  SRR19973065.3351917/1‑150 (MQ=60)
                                                                    GCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGC                                                                                  >  SRR19973065.2059564/1‑150 (MQ=60)
                                                                        GCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGT                                                                              <  SRR19973065.3045171/150‑1 (MQ=60)
                                                                        GCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGT                                                                              <  SRR19973065.2475671/150‑1 (MQ=60)
                                                                           TTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGT                                                                           <  SRR19973065.560005/150‑1 (MQ=60)
                                                                             TAATCGCCAAAGAACAAGCAGAACGCGGCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGA                                                                         <  SRR19973065.1912736/150‑1 (MQ=60)
                                                                             TAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGA                                                                         <  SRR19973065.1912614/150‑1 (MQ=60)
                                                                             TAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGA                                                                         <  SRR19973065.1878139/150‑1 (MQ=60)
                                                                              AATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAA                                                                        <  SRR19973065.1339932/150‑1 (MQ=60)
                                                                               ATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAAT                                                                       <  SRR19973065.2978194/150‑1 (MQ=60)
                                                                               ATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAAT                                                                       <  SRR19973065.2978186/150‑1 (MQ=60)
                                                                                 CGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCT                                                                     <  SRR19973065.2413515/150‑1 (MQ=60)
                                                                                      AAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGG                                                                >  SRR19973065.802541/1‑150 (MQ=60)
                                                                                      AAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGG                                                                >  SRR19973065.551534/1‑150 (MQ=60)
                                                                                       AGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGC                                                               >  SRR19973065.2541816/1‑150 (MQ=60)
                                                                                         AACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCACGGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCG                                                             >  SRR19973065.1949149/1‑150 (MQ=60)
                                                                                         AACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCG                                                             >  SRR19973065.1949130/1‑150 (MQ=60)
                                                                                           CAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCG                                                           >  SRR19973065.1131923/1‑150 (MQ=60)
                                                                                            AAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGA                                                          >  SRR19973065.532882/1‑150 (MQ=60)
                                                                                            AAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGA                                                          >  SRR19973065.532876/1‑150 (MQ=60)
                                                                                               CAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAAC                                                       >  SRR19973065.1770499/1‑150 (MQ=60)
                                                                                               CAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAAC                                                       <  SRR19973065.1199820/150‑1 (MQ=60)
                                                                                               CAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAAC                                                       >  SRR19973065.2879619/1‑150 (MQ=60)
                                                                                                AGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACG                                                      >  SRR19973065.924993/1‑150 (MQ=60)
                                                                                                AGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACG                                                      >  SRR19973065.1190784/1‑150 (MQ=60)
                                                                                                AGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACG                                                      >  SRR19973065.3018443/1‑150 (MQ=60)
                                                                                                   ACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCT                                                   <  SRR19973065.2337165/150‑1 (MQ=60)
                                                                                                   ACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCT                                                   <  SRR19973065.1364035/150‑1 (MQ=60)
                                                                                                   ACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCT                                                   <  SRR19973065.1364330/150‑1 (MQ=60)
                                                                                                   ACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCT                                                   <  SRR19973065.1364356/150‑1 (MQ=60)
                                                                                                   ACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCT                                                   <  SRR19973065.2337049/150‑1 (MQ=60)
                                                                                                   ACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCT                                                   <  SRR19973065.2336949/150‑1 (MQ=60)
                                                                                                           CGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAA                                           >  SRR19973065.2155438/1‑150 (MQ=60)
                                                                                                           CGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAA                                           >  SRR19973065.2155681/1‑150 (MQ=60)
                                                                                                            GGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAAT                                          >  SRR19973065.1734619/1‑150 (MQ=60)
                                                                                                             GCGCAAAGTGACCCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATG                                         <  SRR19973065.1238057/150‑1 (MQ=60)
                                                                                                             GCGCAAAGTGAACCGGAGATCTTACAAAGCGATCCTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATG                                         <  SRR19973065.352496/150‑1 (MQ=60)
                                                                                                              CGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGC                                        <  SRR19973065.1685348/150‑1 (MQ=60)
                                                                                                                     TGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGC                                 >  SRR19973065.2167512/1‑150 (MQ=60)
                                                                                                                     TGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGC                                 >  SRR19973065.78575/1‑150 (MQ=60)
                                                                                                                     TGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGC                                 >  SRR19973065.2167587/1‑150 (MQ=60)
                                                                                                                      GAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCA                                >  SRR19973065.728949/1‑150 (MQ=60)
                                                                                                                             AGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGG                         >  SRR19973065.1592228/1‑150 (MQ=60)
                                                                                                                             AGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGG                         >  SRR19973065.1438056/1‑150 (MQ=60)
                                                                                                                               ATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGT                       >  SRR19973065.1614790/1‑150 (MQ=60)
                                                                                                                               ATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGT                       >  SRR19973065.1616307/1‑150 (MQ=60)
                                                                                                                                   TACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAAC                   <  SRR19973065.1438680/150‑1 (MQ=60)
                                                                                                                                            ATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTG          >  SRR19973065.225145/1‑150 (MQ=60)
                                                                                                                                            ATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTG          >  SRR19973065.224638/1‑150 (MQ=60)
                                                                                                                                              CTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCG        >  SRR19973065.1302437/1‑150 (MQ=60)
                                                                                                                                              CTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCG        >  SRR19973065.1302452/1‑150 (MQ=60)
                                                                                                                                              CTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCG        >  SRR19973065.1303686/1‑150 (MQ=60)
                                                                                                                                              CTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCG        >  SRR19973065.1919630/1‑150 (MQ=60)
                                                                                                                                                  CCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCGCAAG    <  SRR19973065.892317/150‑1 (MQ=60)
                                                                                                                                                    TGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCGCAAGGG  <  SRR19973065.2299144/150‑1 (MQ=60)
                                                                                                                                                    TGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCGCAAGGG  <  SRR19973065.3361986/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCGCAAGGG  >  CP009273/159518‑159815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑