Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 250,909 A→T 100% L432Q (CTG→CAG)  pepD ← aminoacyl‑histidine dipeptidase (peptidase D)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273250,9090AT98.2% 681.2 / 1.8 219L432Q (CTG→CAG) pepDaminoacyl‑histidine dipeptidase (peptidase D)
Reads supporting (aligned to +/- strand):  ref base A (4/0);  new base T (117/97);  total (121/98)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 219 total aligned reads displayed.

TTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTT  >  CP009273/250765‑251053
                                                                                                                                                |                                                                                                                                                
ttcAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGc                                                                                                                                               >  1:2726302/1‑148 (MQ=255)
    gcagTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCcgc                                                                                                                                           >  1:139022/1‑148 (MQ=255)
     cagTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCcgcg                                                                                                                                          <  2:1255028/148‑1 (MQ=255)
          cagtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGAt                                                                                                                                     <  2:3130522/148‑1 (MQ=255)
          cagtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGACCGCGTGGAt                                                                                                                                     <  1:1820849/148‑1 (MQ=255)
           agtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATa                                                                                                                                    >  2:3425591/1‑148 (MQ=255)
            gtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATaa                                                                                                                                   <  2:1014201/148‑1 (MQ=255)
             tcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAAt                                                                                                                                  >  2:368290/1‑148 (MQ=255)
               agcagTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCt                                                                                                                                >  1:1228456/1‑148 (MQ=255)
                gcagTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTg                                                                                                                               <  1:1924412/148‑1 (MQ=255)
                      tCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGtt                                                                                                                         >  1:711679/1‑148 (MQ=255)
                      tCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGtt                                                                                                                         <  1:30546/148‑1 (MQ=255)
                       ccAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTc                                                                                                                        >  2:2295065/1‑148 (MQ=255)
                       ccAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTc                                                                                                                        <  1:2560663/148‑1 (MQ=255)
                        cAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCg                                                                                                                       <  2:3403369/148‑1 (MQ=255)
                            aaTGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGt                                                                                                                   <  2:941908/148‑1 (MQ=255)
                             aTGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTc                                                                                                                  >  1:3304338/1‑148 (MQ=255)
                             aTGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTc                                                                                                                  >  1:1563343/1‑148 (MQ=255)
                              tGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCt                                                                                                                 >  1:2004128/1‑148 (MQ=255)
                              tGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCt                                                                                                                 <  2:8651/148‑1 (MQ=255)
                                 ccTACGCTTTCTATTAGAACTTGCTCATACGGAGACTGTGGACCGATTAAAGTTGGACCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCAAATTCCTCGCACGAGAGGATAATCAGGATGTTCGGCGTCttgt                                                                                                              >  2:1948537/1‑148 (MQ=255)
                                   tACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCttgttg                                                                                                            <  1:392709/148‑1 (MQ=255)
                                    aCGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGa                                                                                                           >  1:602206/1‑148 (MQ=255)
                                      gCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAAc                                                                                                         >  1:3476617/1‑148 (MQ=255)
                                      gCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAAc                                                                                                         <  1:1870205/148‑1 (MQ=255)
                                      gCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAAc                                                                                                         >  1:129270/1‑148 (MQ=255)
                                       cTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACa                                                                                                        <  2:2566804/148‑1 (MQ=255)
                                       cTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACa                                                                                                        <  2:2683200/148‑1 (MQ=255)
                                        tttCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAg                                                                                                       >  2:2009914/1‑148 (MQ=255)
                                         ttCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAgg                                                                                                      >  2:752834/1‑148 (MQ=255)
                                              tgtgAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATGTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTg                                                                                                 <  2:2591235/148‑1 (MQ=255)
                                                tgAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGAt                                                                                               <  2:502804/148‑1 (MQ=255)
                                                   aCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAgg                                                                                            >  2:2867609/1‑148 (MQ=255)
                                                   aCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAgg                                                                                            >  2:2867500/1‑148 (MQ=255)
                                                     ttGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGtt                                                                                          >  2:345576/1‑148 (MQ=255)
                                                      tGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGttt                                                                                         >  2:645742/1‑148 (MQ=255)
                                                        cTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCa                                                                                       >  1:1130763/1‑148 (MQ=255)
                                                        cTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCa                                                                                       <  1:3425591/148‑1 (MQ=255)
                                                         tCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCAc                                                                                      >  1:922120/1‑148 (MQ=255)
                                                          cATCCGTAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACg                                                                                     <  2:2260860/148‑1 (MQ=255)
                                                            tCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTa                                                                                   <  1:3132055/148‑1 (MQ=255)
                                                             ccGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTAc                                                                                  <  1:2230809/148‑1 (MQ=255)
                                                               ggAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCa                                                                                >  2:604440/1‑148 (MQ=255)
                                                                gagaGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAg                                                                               >  1:606242/1‑148 (MQ=255)
                                                                gagaGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAg                                                                               >  1:606286/1‑148 (MQ=255)
                                                                gagaGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAg                                                                               >  1:147560/1‑148 (MQ=255)
                                                                  gagTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGAt                                                                             >  1:2900451/1‑148 (MQ=255)
                                                                  gagTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGAt                                                                             <  1:1137116/148‑1 (MQ=255)
                                                                   agTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATg                                                                            >  2:2143202/1‑148 (MQ=255)
                                                                    gtgtGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGc                                                                           <  1:618917/148‑1 (MQ=255)
                                                                     tgtgGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCa                                                                          >  2:1460035/1‑148 (MQ=255)
                                                                      gtgGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCAt                                                                         <  2:2797070/148‑1 (MQ=255)
                                                                         gACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCAc                                                                      >  2:612334/1‑148 (MQ=255)
                                                                          aCCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCAcc                                                                     >  1:839460/1‑148 (MQ=255)
                                                                               tGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGgaga                                                                <  1:650607/148‑1 (MQ=255)
                                                                                  tAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACACTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAAtt                                                             <  1:1733070/148‑1 (MQ=255)
                                                                                   aGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTa                                                            >  2:3063635/1‑148 (MQ=255)
                                                                                     ttGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGc                                                          >  2:2105371/1‑148 (MQ=255)
                                                                                      tGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCg                                                         <  1:1013653/148‑1 (MQ=255)
                                                                                       ggCCCGATAGACACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGt                                                        <  2:3091010/148‑1 (MQ=255)
                                                                                         cccGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTcc                                                      >  1:2475517/1‑148 (MQ=255)
                                                                                             aTAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCt                                                  >  1:2502217/1‑148 (MQ=255)
                                                                                              tAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTg                                                 >  1:2991043/1‑148 (MQ=255)
                                                                                                gAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgcc                                               >  1:2063739/1‑148 (MQ=255)
                                                                                                 aaaCCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgcca                                              >  2:2807769/1‑148 (MQ=255)
                                                                                                     cATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGTTTGTTCGGCGTCTTGTTGAAAAGGGGCTGATAGGTTTCAGGTACCAGTTGCATCACCGGAGAATTAGCGTCCGGCTgccagcca                                          <  2:3011788/148‑1 (MQ=255)
                                                                                                      aTGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGTATAATCTGGATGTTCGGCGTCTTGTTGAACACGCGCAGATAGGATTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGccagccag                                         >  2:3464698/1‑148 (MQ=255)
                                                                                                       tGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAgg                                        >  1:1560565/1‑148 (MQ=255)
                                                                                                        gTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGa                                       <  2:1050274/148‑1 (MQ=255)
                                                                                                        gTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGa                                       >  2:2434972/1‑148 (MQ=255)
                                                                                                        gTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGa                                       >  1:3461109/1‑148 (MQ=255)
                                                                                                         tCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGat                                      <  1:2438694/148‑1 (MQ=255)
                                                                                                           cATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGatat                                    <  1:824624/148‑1 (MQ=255)
                                                                                                            aTTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTAACAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATg                                   >  2:2477534/1‑148 (MQ=255)
                                                                                                             tttCCGTATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATgc                                  >  1:174261/1‑148 (MQ=255)
                                                                                                              ttCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATgcg                                 >  2:2190376/1‑148 (MQ=255)
                                                                                                              ttCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATgcg                                 >  2:422239/1‑148 (MQ=255)
                                                                                                               tCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATgcgc                                <  1:3370493/148‑1 (MQ=255)
                                                                                                                  ggATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCtt                             <  2:1826058/148‑1 (MQ=255)
                                                                                                                    aTACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTc                           >  1:705731/1‑148 (MQ=255)
                                                                                                                    aTACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTc                           >  2:1077971/1‑148 (MQ=255)
                                                                                                                     tACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACTTACCAGATGCATCACAGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCg                          >  1:1066192/1‑148 (MQ=255)
                                                                                                                     tACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCg                          >  2:1510117/1‑148 (MQ=255)
                                                                                                                         gTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTc                      >  2:963312/1‑148 (MQ=255)
                                                                                                                          ttttttGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCg                     <  1:851818/148‑1 (MQ=255)
                                                                                                                          ttttttGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCg                     <  1:2692142/148‑1 (MQ=255)
                                                                                                                          ttttttGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCg                     >  1:2423448/1‑148 (MQ=255)
                                                                                                                                    aGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCgcgc           >  2:1932760/1‑148 (MQ=255)
                                                                                                                                     gACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgcc          >  2:3270718/1‑148 (MQ=255)
                                                                                                                                     gACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgcc          <  1:1023653/148‑1 (MQ=255)
                                                                                                                                          caTTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgccagcca     >  1:2377776/1‑148 (MQ=255)
                                                                                                                                           aTTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGccagccag    >  1:2113895/1‑148 (MQ=255)
                                                                                                                                            ttCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGt   <  2:2552362/148‑1 (MQ=255)
                                                                                                                                            ttCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGt   <  2:1883824/148‑1 (MQ=255)
                                                                                                                                            ttCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGt   <  2:1790682/148‑1 (MQ=255)
                                                                                                                                            ttCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGt   <  2:1312319/148‑1 (MQ=255)
                                                                                                                                            ttCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGt   <  2:1312167/148‑1 (MQ=255)
                                                                                                                                            ttCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGt   >  1:2286971/1‑148 (MQ=255)
                                                                                                                                             tCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGtt  >  2:3111487/1‑148 (MQ=255)
                                                                                                                                             tCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGtt  >  2:839629/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTT  >  CP009273/250765‑251053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACC  >  CP009273/250760‑251057
                                                                                                                                                     |                                                                                                                                                    
TTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCT                                                                                                                                                      >  SRR19973065.3030335/1‑150 (MQ=60)
TTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCT                                                                                                                                                      <  SRR19973065.685141/150‑1 (MQ=60)
TTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCCGAGAGTGTGGACCGGTGATAGTTGGCACGATAGAAACCATGTCCATTTCCGGATAAGGTTTTTTGAACAGACCACATTCCT                                                                                                                                                      >  SRR19973065.3030229/1‑150 (MQ=60)
 TTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTG                                                                                                                                                     <  SRR19973065.563692/150‑1 (MQ=60)
 TTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTG                                                                                                                                                     <  SRR19973065.563876/150‑1 (MQ=60)
 TTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTG                                                                                                                                                     <  SRR19973065.1692380/150‑1 (MQ=60)
 TTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTG                                                                                                                                                     >  SRR19973065.1353339/1‑150 (MQ=60)
   CTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGC                                                                                                                                                   >  SRR19973065.2743258/1‑150 (MQ=60)
   CTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGC                                                                                                                                                   <  SRR19973065.1358931/150‑1 (MQ=60)
      TCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCG                                                                                                                                                >  SRR19973065.2568335/1‑150 (MQ=60)
      TCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCG                                                                                                                                                >  SRR19973065.1802423/1‑150 (MQ=60)
       CAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGC                                                                                                                                               >  SRR19973065.140210/1‑150 (MQ=60)
        AGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCG                                                                                                                                              >  SRR19973065.3401252/1‑150 (MQ=60)
               CAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGACCGCGTGGATAA                                                                                                                                       <  SRR19973065.1832837/150‑1 (MQ=60)
                 GTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATC                                                                                                                                     <  SRR19973065.1837483/150‑1 (MQ=60)
                 GTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATC                                                                                                                                     <  SRR19973065.1138805/150‑1 (MQ=60)
                  TCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCT                                                                                                                                    >  SRR19973065.1236474/1‑150 (MQ=60)
                     GCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGA                                                                                                                                 <  SRR19973065.1937025/150‑1 (MQ=60)
                      CAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGAT                                                                                                                                >  SRR19973065.3477558/1‑150 (MQ=60)
                         TGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTT                                                                                                                             >  SRR19973065.716439/1‑150 (MQ=60)
                           TCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCG                                                                                                                           <  SRR19973065.30935/150‑1 (MQ=60)
                            CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGG                                                                                                                          <  SRR19973065.2576675/150‑1 (MQ=60)
                              AGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCG                                                                                                                        <  SRR19973065.3088828/150‑1 (MQ=60)
                                TAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTC                                                                                                                      >  SRR19973065.3324467/1‑150 (MQ=60)
                                TAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTC                                                                                                                      >  SRR19973065.1573733/1‑150 (MQ=60)
                                 AATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCT                                                                                                                     >  SRR19973065.2017084/1‑150 (MQ=60)
                                 AATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCT                                                                                                                     >  SRR19973065.233405/1‑150 (MQ=60)
                                 AATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCT                                                                                                                     >  SRR19973065.652430/1‑150 (MQ=60)
                                       CTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGA                                                                                                               >  SRR19973065.605982/1‑150 (MQ=60)
                                       CTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGA                                                                                                               >  SRR19973065.605987/1‑150 (MQ=60)
                                        TACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAA                                                                                                              <  SRR19973065.395451/150‑1 (MQ=60)
                                         ACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAAC                                                                                                             >  SRR19973065.3497772/1‑150 (MQ=60)
                                         ACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAAC                                                                                                             >  SRR19973065.3114987/1‑150 (MQ=60)
                                         ACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAAC                                                                                                             >  SRR19973065.130381/1‑150 (MQ=60)
                                           GCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAG                                                                                                           <  SRR19973065.1882514/150‑1 (MQ=60)
                                                CGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCT                                                                                                      <  SRR19973065.753473/150‑1 (MQ=60)
                                                CGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCT                                                                                                      <  SRR19973065.207749/150‑1 (MQ=60)
                                                 GATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTG                                                                                                     <  SRR19973065.2186865/150‑1 (MQ=60)
                                                   TGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGAT                                                                                                   <  SRR19973065.3303247/150‑1 (MQ=60)
                                                           TGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCA                                                                                           >  SRR19973065.1138109/1‑150 (MQ=60)
                                                            GCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCAC                                                                                          >  SRR19973065.928164/1‑150 (MQ=60)
                                                             CTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACG                                                                                         <  SRR19973065.3446497/150‑1 (MQ=60)
                                                               CATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTA                                                                                       <  SRR19973065.880962/150‑1 (MQ=60)
                                                                 TCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACC                                                                                     <  SRR19973065.3151107/150‑1 (MQ=60)
                                                                  CCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCA                                                                                    <  SRR19973065.2245006/150‑1 (MQ=60)
                                                                  CCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCA                                                                                    <  SRR19973065.1937091/150‑1 (MQ=60)
                                                                   CGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAG                                                                                   >  SRR19973065.610128/1‑150 (MQ=60)
                                                                   CGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAG                                                                                   >  SRR19973065.610132/1‑150 (MQ=60)
                                                                   CGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAG                                                                                   >  SRR19973065.610088/1‑150 (MQ=60)
                                                                   CGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAG                                                                                   >  SRR19973065.148807/1‑150 (MQ=60)
                                                                   CGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAG                                                                                   >  SRR19973065.610941/1‑150 (MQ=60)
                                                                     GAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGAT                                                                                 >  SRR19973065.2918143/1‑150 (MQ=60)
                                                                       GAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGC                                                                               <  SRR19973065.85201/150‑1 (MQ=60)
                                                                       GAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGC                                                                               <  SRR19973065.85243/150‑1 (MQ=60)
                                                                       GAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGC                                                                               <  SRR19973065.1144484/150‑1 (MQ=60)
                                                                         GTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCAT                                                                             <  SRR19973065.622897/150‑1 (MQ=60)
                                                                         GTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCAT                                                                             <  SRR19973065.1021016/150‑1 (MQ=60)
                                                                             GGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACC                                                                         >  SRR19973065.844926/1‑150 (MQ=60)
                                                                             GGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACC                                                                         >  SRR19973065.844573/1‑150 (MQ=60)
                                                                               ACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGG                                                                       >  SRR19973065.117356/1‑150 (MQ=60)
                                                                                  GGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGA                                                                    >  SRR19973065.1426989/1‑150 (MQ=60)
                                                                                    TGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAAT                                                                  <  SRR19973065.654889/150‑1 (MQ=60)
                                                                                      ATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTA                                                                <  SRR19973065.1506609/150‑1 (MQ=60)
                                                                                       TAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACACTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAG                                                               <  SRR19973065.1744538/150‑1 (MQ=60)
                                                                                        AGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGC                                                              <  SRR19973065.1577161/150‑1 (MQ=60)
                                                                                           TGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTC                                                           <  SRR19973065.1020369/150‑1 (MQ=60)
                                                                                            GGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGTCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCC                                                          >  SRR19973065.2490822/1‑150 (MQ=60)
                                                                                            GGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCC                                                          >  SRR19973065.2491103/1‑150 (MQ=60)
                                                                                                CGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCT                                                      >  SRR19973065.2517943/1‑150 (MQ=60)
                                                                                                 GATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTG                                                     >  SRR19973065.3009353/1‑150 (MQ=60)
                                                                                                  ATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGC                                                    <  SRR19973065.3040575/150‑1 (MQ=60)
                                                                                                   TAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCC                                                   >  SRR19973065.2076899/1‑150 (MQ=60)
                                                                                                    AGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCA                                                  <  SRR19973065.2516607/150‑1 (MQ=60)
                                                                                                    AGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCA                                                  >  SRR19973065.3512862/1‑150 (MQ=60)
                                                                                                     GAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAG                                                 >  SRR19973065.1903557/1‑150 (MQ=60)
                                                                                                        ACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCAACACCGGAGAATTAGCGTCCGGCTGCCAGCCA                                              <  SRR19973065.423435/150‑1 (MQ=60)
                                                                                                          CATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGG                                            >  SRR19973065.1570932/1‑150 (MQ=60)
                                                                                                          CATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGG                                            >  SRR19973065.1572363/1‑150 (MQ=60)
                                                                                                          CATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGG                                            >  SRR19973065.1589946/1‑150 (MQ=60)
                                                                                                           ATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGA                                           >  SRR19973065.3484260/1‑150 (MQ=60)
                                                                                                           ATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGA                                           >  SRR19973065.3482196/1‑150 (MQ=60)
                                                                                                              TCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATAT                                        <  SRR19973065.2454070/150‑1 (MQ=60)
                                                                                                              TCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATAT                                        <  SRR19973065.2453676/150‑1 (MQ=60)
                                                                                                                CATTTCCGTATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGC                                      >  SRR19973065.175649/1‑150 (MQ=60)
                                                                                                                CATTTCCGTATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGC                                      >  SRR19973065.175617/1‑150 (MQ=60)
                                                                                                                CATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGC                                      <  SRR19973065.2646106/150‑1 (MQ=60)
                                                                                                                CATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGC                                      <  SRR19973065.830033/150‑1 (MQ=60)
                                                                                                                CATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGC                                      <  SRR19973065.2645982/150‑1 (MQ=60)
                                                                                                                CATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGC                                      <  SRR19973065.1322884/150‑1 (MQ=60)
                                                                                                                CATTTCCGGATACGGTTTTTTGAACAGACCACATTCCGGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGC                                      <  SRR19973065.2970661/150‑1 (MQ=60)
                                                                                                                    TCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCT                                  <  SRR19973065.3391107/150‑1 (MQ=60)
                                                                                                                    TCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCT                                  <  SRR19973065.3390544/150‑1 (MQ=60)
                                                                                                                       GGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTC                               >  SRR19973065.646285/1‑150 (MQ=60)
                                                                                                                       GGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTC                               >  SRR19973065.710554/1‑150 (MQ=60)
                                                                                                                       GGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTC                               >  SRR19973065.710435/1‑150 (MQ=60)
                                                                                                                       GGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTC                               >  SRR19973065.2407698/1‑150 (MQ=60)
                                                                                                                        GATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACTTACCAGATGCATCACAGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCG                              >  SRR19973065.1073254/1‑150 (MQ=60)
                                                                                                                        GATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCG                              >  SRR19973065.1947486/1‑150 (MQ=60)
                                                                                                                        GATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCG                              >  SRR19973065.1074551/1‑150 (MQ=60)
                                                                                                                             GGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCG                         >  SRR19973065.2438731/1‑150 (MQ=60)
                                                                                                                              GTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGG                        >  SRR19973065.694244/1‑150 (MQ=60)
                                                                                                                               TTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGT                       <  SRR19973065.2711627/150‑1 (MQ=60)
                                                                                                                               TTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGT                       <  SRR19973065.2708859/150‑1 (MQ=60)
                                                                                                                               TTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGT                       <  SRR19973065.857331/150‑1 (MQ=60)
                                                                                                                                TTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGATAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTT                      <  SRR19973065.2277955/150‑1 (MQ=60)
                                                                                                                                TTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTT                      <  SRR19973065.3492781/150‑1 (MQ=60)
                                                                                                                                      AACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCG                >  SRR19973065.1238253/1‑150 (MQ=60)
                                                                                                                                      AACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCG                >  SRR19973065.1034190/1‑150 (MQ=60)
                                                                                                                                          GACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAG            <  SRR19973065.1030431/150‑1 (MQ=60)
                                                                                                                                           ACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGC           <  SRR19973065.2816197/150‑1 (MQ=60)
                                                                                                                                             CACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCA         >  SRR19973065.2394692/1‑150 (MQ=60)
                                                                                                                                             CACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCA         >  SRR19973065.2392637/1‑150 (MQ=60)
                                                                                                                                              ACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAG        >  SRR19973065.2127327/1‑150 (MQ=60)
                                                                                                                                              ACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAG        >  SRR19973065.2127501/1‑150 (MQ=60)
                                                                                                                                               CATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGT       >  SRR19973065.2301385/1‑150 (MQ=60)
                                                                                                                                                ATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTT      >  SRR19973065.1249482/1‑150 (MQ=60)
                                                                                                                                                   CCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTAC   <  SRR19973065.2728375/150‑1 (MQ=60)
                                                                                                                                                   CCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTAC   <  SRR19973065.2532090/150‑1 (MQ=60)
                                                                                                                                                   CCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTAC   <  SRR19973065.2532070/150‑1 (MQ=60)
                                                                                                                                                    CTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAGCAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACC  >  SRR19973065.3275125/1‑150 (MQ=60)
                                                                                                                                                    CTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACC  >  SRR19973065.2031473/1‑150 (MQ=60)
                                                                                                                                                    CTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACC  >  SRR19973065.2031484/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACC  >  CP009273/250760‑251057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑