Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 279,334 C→A 100% I141I (ATC→ATA)  yagF → CP4‑6 prophage; dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273279,3340CA99.4% 609.5 / ‑4.2 193I141I (ATC→ATA) yagFCP4‑6 prophage; dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (86/105);  total (87/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 193 total aligned reads displayed.

GCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACG  >  CP009273/279189‑279478
                                                                                                                                                 |                                                                                                                                                
 cATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgtg                                                                                                                                               >  2:3247246/1‑148 (MQ=255)
 cATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgtg                                                                                                                                               >  2:1448907/1‑148 (MQ=255)
  atgcagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgtgt                                                                                                                                              <  2:2770481/148‑1 (MQ=255)
  atgcagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgtgt                                                                                                                                              <  2:1758124/148‑1 (MQ=255)
  atgcagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgtgt                                                                                                                                              <  2:1757930/148‑1 (MQ=255)
     cagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTc                                                                                                                                           >  1:2484344/1‑148 (MQ=255)
       gatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcgc                                                                                                                                         <  1:1132630/148‑1 (MQ=255)
       gatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcgc                                                                                                                                         >  1:1600002/1‑148 (MQ=255)
        atgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTcgcc                                                                                                                                        >  2:973923/1‑148 (MQ=255)
         tgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcgccg                                                                                                                                       <  1:1243586/148‑1 (MQ=255)
          gcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcgccgc                                                                                                                                      >  2:2062732/1‑148 (MQ=255)
           cagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCgccgcc                                                                                                                                     <  1:2669600/148‑1 (MQ=255)
             ggcggcggcGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTg                                                                                                                                   >  2:3229590/1‑148 (MQ=255)
              gcggcggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGa                                                                                                                                  <  2:625774/148‑1 (MQ=255)
                ggcggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATc                                                                                                                                <  2:2453161/148‑1 (MQ=255)
                ggcggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATc                                                                                                                                <  2:2450350/148‑1 (MQ=255)
                  cggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCg                                                                                                                              <  1:2183088/148‑1 (MQ=255)
                     cgAAGGAGATCACCCGCAATGGCGGGATCCCGGTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTc                                                                                                                           <  2:2148583/148‑1 (MQ=255)
                       aaGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTccc                                                                                                                         <  2:68882/148‑1 (MQ=255)
                       aaGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTccc                                                                                                                         >  1:2201577/1‑148 (MQ=255)
                       aaGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTccc                                                                                                                         >  1:431978/1‑148 (MQ=255)
                         ggAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTg                                                                                                                       >  1:755762/1‑148 (MQ=255)
                             aTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGa                                                                                                                   >  1:730404/1‑148 (MQ=255)
                             aTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGa                                                                                                                   >  1:2805093/1‑148 (MQ=255)
                               cACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGAcg                                                                                                                 >  2:2962544/1‑148 (MQ=255)
                               cACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGAcg                                                                                                                 >  1:2576199/1‑148 (MQ=255)
                               cACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGAcg                                                                                                                 >  2:1536202/1‑148 (MQ=255)
                                 cccGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTCTGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGAcgcg                                                                                                               >  2:294530/1‑148 (MQ=255)
                                 cccGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGAcgcg                                                                                                               <  2:712640/148‑1 (MQ=255)
                                      aaTGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCggcggg                                                                                                          >  1:619621/1‑148 (MQ=255)
                                          gCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGt                                                                                                      <  1:2973499/148‑1 (MQ=255)
                                             ggATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGAt                                                                                                   >  2:2559657/1‑148 (MQ=255)
                                             ggATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGAt                                                                                                   <  1:3314690/148‑1 (MQ=255)
                                              gATCCCGGTCTCGTCAGTCTTCAGCGATCCGTCCGAAGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGGCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATc                                                                                                  <  2:1247003/148‑1 (MQ=255)
                                                tCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCgg                                                                                                <  1:1917706/148‑1 (MQ=255)
                                                tCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCgg                                                                                                >  2:1166628/1‑148 (MQ=255)
                                                tCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCgg                                                                                                >  2:209700/1‑148 (MQ=255)
                                                 cccGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCACAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGc                                                                                               <  1:2011102/148‑1 (MQ=255)
                                                  ccGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCg                                                                                              <  2:2286965/148‑1 (MQ=255)
                                                   cGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGt                                                                                             <  1:248748/148‑1 (MQ=255)
                                                    gTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTa                                                                                            >  1:2589858/1‑148 (MQ=255)
                                                     ttCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAg                                                                                           >  1:349891/1‑148 (MQ=255)
                                                      tCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGc                                                                                          >  1:1836202/1‑148 (MQ=255)
                                                      tCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGc                                                                                          >  1:3391092/1‑148 (MQ=255)
                                                         cgGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGAc                                                                                       <  2:3367823/148‑1 (MQ=255)
                                                          ggCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGAcc                                                                                      <  2:1700786/148‑1 (MQ=255)
                                                            ccTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTg                                                                                    >  2:1584162/1‑148 (MQ=255)
                                                            ccTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTg                                                                                    >  2:1584178/1‑148 (MQ=255)
                                                            ccTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTg                                                                                    >  1:2700865/1‑148 (MQ=255)
                                                             cTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTCGCGACCTGc                                                                                   >  2:2976487/1‑148 (MQ=255)
                                                             cTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGACGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACATGc                                                                                   >  2:619589/1‑148 (MQ=255)
                                                               tCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGa                                                                                 <  1:1440965/148‑1 (MQ=255)
                                                                 gTCAGCGATCCGTGCGACGGGCGCTCGCCGGGCACGCACGGTATGTTCGAGTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATa                                                                               <  1:1187629/148‑1 (MQ=255)
                                                                 gTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATa                                                                               <  1:1493453/148‑1 (MQ=255)
                                                                 gTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATa                                                                               <  1:1187306/148‑1 (MQ=255)
                                                                   cAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaaa                                                                             >  2:3083597/1‑148 (MQ=255)
                                                                   cAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaaa                                                                             >  1:1182695/1‑148 (MQ=255)
                                                                    aGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAg                                                                            <  1:1892430/148‑1 (MQ=255)
                                                                     gCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAgg                                                                           >  2:291810/1‑148 (MQ=255)
                                                                     gCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAgg                                                                           <  1:3323970/148‑1 (MQ=255)
                                                                      cGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAggg                                                                          <  2:2090223/148‑1 (MQ=255)
                                                                      cGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAggg                                                                          <  1:1025912/148‑1 (MQ=255)
                                                                      cGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAggg                                                                          >  2:3180022/1‑148 (MQ=255)
                                                                         tCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTg                                                                       >  2:1895136/1‑148 (MQ=255)
                                                                         tCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTg                                                                       <  2:2843017/148‑1 (MQ=255)
                                                                         tCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTg                                                                       >  2:2265458/1‑148 (MQ=255)
                                                                           cGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGcc                                                                     <  2:2618737/148‑1 (MQ=255)
                                                                              gCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGc                                                                  <  1:3086643/148‑1 (MQ=255)
                                                                                  cGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGccacc                                                              <  1:1314814/148‑1 (MQ=255)
                                                                                  cGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGccacc                                                              <  1:641325/148‑1 (MQ=255)
                                                                                  cGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGccacc                                                              <  1:2400719/148‑1 (MQ=255)
                                                                                  cGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGccacc                                                              <  1:2632049/148‑1 (MQ=255)
                                                                                    ggCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCAt                                                            >  1:1066409/1‑148 (MQ=255)
                                                                                    ggCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCAt                                                            <  2:2533821/148‑1 (MQ=255)
                                                                                     gcgcTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATg                                                           <  2:1635816/148‑1 (MQ=255)
                                                                                     gcgcTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATg                                                           >  1:3175695/1‑148 (MQ=255)
                                                                                        cTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGAtt                                                        <  2:515800/148‑1 (MQ=255)
                                                                                         tCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTg                                                       <  2:428100/148‑1 (MQ=255)
                                                                                                gcacgcacGGTATGTTCGAGTCCCTGCCGTACCGCAACGACGCGGCGCTAGTGTGTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGc                                                <  2:3152045/148‑1 (MQ=255)
                                                                                                       cgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATg                                         <  1:1157190/148‑1 (MQ=255)
                                                                                                        ggTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGc                                        <  1:1820267/148‑1 (MQ=255)
                                                                                                           aTGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACg                                     >  2:882756/1‑148 (MQ=255)
                                                                                                              ttCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGAcc                                  >  2:3352348/1‑148 (MQ=255)
                                                                                                                cGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTg                                <  1:3247246/148‑1 (MQ=255)
                                                                                                                cGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTg                                >  1:2164540/1‑148 (MQ=255)
                                                                                                                   ttCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCg                             >  2:796561/1‑148 (MQ=255)
                                                                                                                    tCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGa                            <  2:1275391/148‑1 (MQ=255)
                                                                                                                       cTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTa                         <  2:1795902/148‑1 (MQ=255)
                                                                                                                        tGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTAt                        <  2:3251546/148‑1 (MQ=255)
                                                                                                                          ccGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTc                      >  1:1389372/1‑148 (MQ=255)
                                                                                                                          ccGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCACTGGCCGCGATGCACGACCTGCCGACTATTc                      >  2:586411/1‑148 (MQ=255)
                                                                                                                           cGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCt                     >  1:827223/1‑148 (MQ=255)
                                                                                                                            gTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTg                    <  1:2859392/148‑1 (MQ=255)
                                                                                                                             tACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTgg                   >  2:1076336/1‑148 (MQ=255)
                                                                                                                                  cAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcg              >  1:2622539/1‑148 (MQ=255)
                                                                                                                                       acgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggcgg         >  1:3408095/1‑148 (MQ=255)
                                                                                                                                          cggcgATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCgggcggggc      <  1:290907/148‑1 (MQ=255)
                                                                                                                                          cggcgATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCgggcggggc      <  1:291810/148‑1 (MQ=255)
                                                                                                                                           ggcgAGAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGGGCCgggcggggcg     <  1:1992686/148‑1 (MQ=255)
                                                                                                                                              gATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACg  <  1:1204548/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACG  >  CP009273/279189‑279478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCC  >  CP009273/279185‑279483
                                                                                                                                                     |                                                                                                                                                     
ATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATA                                                                                                                                                       <  SRR19973065.524445/150‑1 (MQ=60)
  CGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGT                                                                                                                                                     <  SRR19973065.1046751/150‑1 (MQ=60)
   GGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCACCGACGCGGCGATAGTG                                                                                                                                                    >  SRR19973065.2455914/1‑150 (MQ=60)
     CATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTT                                                                                                                                                  >  SRR19973065.670426/1‑150 (MQ=60)
     CATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTT                                                                                                                                                  >  SRR19973065.2600346/1‑150 (MQ=60)
       TGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTC                                                                                                                                                >  SRR19973065.2499978/1‑150 (MQ=60)
         CAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGC                                                                                                                                              >  SRR19973065.1610694/1‑150 (MQ=60)
           GATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCG                                                                                                                                            <  SRR19973065.1139968/150‑1 (MQ=60)
           GATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCG                                                                                                                                            <  SRR19973065.1139978/150‑1 (MQ=60)
             TGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCC                                                                                                                                          <  SRR19973065.1251765/150‑1 (MQ=60)
             TGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCC                                                                                                                                          <  SRR19973065.1251764/150‑1 (MQ=60)
               CAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTG                                                                                                                                        <  SRR19973065.2686134/150‑1 (MQ=60)
                      CGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCT                                                                                                                                 <  SRR19973065.2197095/150‑1 (MQ=60)
                      CGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCT                                                                                                                                 <  SRR19973065.2196977/150‑1 (MQ=60)
                         CGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCC                                                                                                                              >  SRR19973065.2215674/1‑150 (MQ=60)
                         CGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCC                                                                                                                              >  SRR19973065.2214360/1‑150 (MQ=60)
                         CGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCC                                                                                                                              >  SRR19973065.434980/1‑150 (MQ=60)
                           AAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTG                                                                                                                            >  SRR19973065.760800/1‑150 (MQ=60)
                              GAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCG                                                                                                                         >  SRR19973065.2923535/1‑150 (MQ=60)
                               AGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGA                                                                                                                        >  SRR19973065.1992409/1‑150 (MQ=60)
                               AGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGA                                                                                                                        >  SRR19973065.2822456/1‑150 (MQ=60)
                               AGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGA                                                                                                                        >  SRR19973065.735298/1‑150 (MQ=60)
                               AGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGA                                                                                                                        >  SRR19973065.2823131/1‑150 (MQ=60)
                               AGATCACCCGCAATGGCGGGATCCCGTTCGCCGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGA                                                                                                                        >  SRR19973065.2506971/1‑150 (MQ=60)
                                 ATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACG                                                                                                                      >  SRR19973065.2592267/1‑150 (MQ=60)
                                   CACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCG                                                                                                                    <  SRR19973065.213782/150‑1 (MQ=60)
                                   CACCCGCAAGGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGAGTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCG                                                                                                                    <  SRR19973065.1590438/150‑1 (MQ=60)
                                        GCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGG                                                                                                               >  SRR19973065.623604/1‑150 (MQ=60)
                                              GCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGA                                                                                                         <  SRR19973065.2988936/150‑1 (MQ=60)
                                              GCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGA                                                                                                         <  SRR19973065.2991686/150‑1 (MQ=60)
                                                GGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATC                                                                                                       <  SRR19973065.3214220/150‑1 (MQ=60)
                                                 GGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCG                                                                                                      <  SRR19973065.2015759/150‑1 (MQ=60)
                                                 GGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCG                                                                                                      <  SRR19973065.3335005/150‑1 (MQ=60)
                                                 GGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCG                                                                                                      <  SRR19973065.3334900/150‑1 (MQ=60)
                                                    TCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCG                                                                                                   <  SRR19973065.1930285/150‑1 (MQ=60)
                                                     CCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCACAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGT                                                                                                  <  SRR19973065.2024075/150‑1 (MQ=60)
                                                      CCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTA                                                                                                 >  SRR19973065.2605975/1‑150 (MQ=60)
                                                      CCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTA                                                                                                 >  SRR19973065.2606019/1‑150 (MQ=60)
                                                       CGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAG                                                                                                >  SRR19973065.352340/1‑150 (MQ=60)
                                                       CGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAG                                                                                                <  SRR19973065.250499/150‑1 (MQ=60)
                                                       CGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAG                                                                                                <  SRR19973065.250453/150‑1 (MQ=60)
                                                        GTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGC                                                                                               >  SRR19973065.3411836/1‑150 (MQ=60)
                                                        GTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGC                                                                                               >  SRR19973065.1848310/1‑150 (MQ=60)
                                                        GTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGC                                                                                               >  SRR19973065.589860/1‑150 (MQ=60)
                                                              GGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTG                                                                                         >  SRR19973065.2717645/1‑150 (MQ=60)
                                                                CCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCG                                                                                       <  SRR19973065.3344449/150‑1 (MQ=60)
                                                                   TCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATA                                                                                    <  SRR19973065.1450300/150‑1 (MQ=60)
                                                                     GTCAGCGATCCGTGCGACGGGCGCTCGCCGGGCACGCACGGTATGTTCGAGTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAA                                                                                  <  SRR19973065.1195138/150‑1 (MQ=60)
                                                                     GTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAA                                                                                  >  SRR19973065.1190185/1‑150 (MQ=60)
                                                                     GTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAA                                                                                  <  SRR19973065.1194814/150‑1 (MQ=60)
                                                                     GTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAA                                                                                  <  SRR19973065.1195130/150‑1 (MQ=60)
                                                                     GTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAA                                                                                  <  SRR19973065.1503044/150‑1 (MQ=60)
                                                                     GTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAA                                                                                  <  SRR19973065.1502543/150‑1 (MQ=60)
                                                                       CAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGG                                                                                <  SRR19973065.2259644/150‑1 (MQ=60)
                                                                        AGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAATGGG                                                                               >  SRR19973065.367665/1‑150 (MQ=60)
                                                                        AGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGG                                                                               <  SRR19973065.1905341/150‑1 (MQ=60)
                                                                        AGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGG                                                                               <  SRR19973065.1904870/150‑1 (MQ=60)
                                                                        AGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGATGATCGGCGTAGCGACCTGCGATAAAGGG                                                                               >  SRR19973065.832273/1‑150 (MQ=60)
                                                                         GCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGC                                                                              <  SRR19973065.3344254/150‑1 (MQ=60)
                                                                          CGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCT                                                                             <  SRR19973065.1032699/150‑1 (MQ=60)
                                                                          CGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCT                                                                             <  SRR19973065.745943/150‑1 (MQ=60)
                                                                           GATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTG                                                                            <  SRR19973065.2703143/150‑1 (MQ=60)
                                                                                  GCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCA                                                                     <  SRR19973065.3105492/150‑1 (MQ=60)
                                                                                     ACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCA                                                                  <  SRR19973065.1174846/150‑1 (MQ=60)
                                                                                      CGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCAT                                                                 <  SRR19973065.1322999/150‑1 (MQ=60)
                                                                                      CGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCAT                                                                 <  SRR19973065.2415815/150‑1 (MQ=60)
                                                                                      CGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCAT                                                                 <  SRR19973065.645437/150‑1 (MQ=60)
                                                                                      CGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCAT                                                                 <  SRR19973065.645535/150‑1 (MQ=60)
                                                                                      CGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCAT                                                                 >  SRR19973065.1073472/1‑150 (MQ=60)
                                                                                      CGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCAT                                                                 <  SRR19973065.1323508/150‑1 (MQ=60)
                                                                                      CGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCAT                                                                 <  SRR19973065.2648317/150‑1 (MQ=60)
                                                                                       GGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATG                                                                >  SRR19973065.3194894/1‑150 (MQ=60)
                                                                                                CAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTG                                                       >  SRR19973065.1765617/1‑150 (MQ=60)
                                                                                                        GCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGAT                                               <  SRR19973065.3483099/150‑1 (MQ=60)
                                                                                                           CGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCA                                            <  SRR19973065.1164572/150‑1 (MQ=60)
                                                                                                           CGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCA                                            <  SRR19973065.1164615/150‑1 (MQ=60)
                                                                                                            GGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCAC                                           <  SRR19973065.1832253/150‑1 (MQ=60)
                                                                                                            GGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCAC                                           >  SRR19973065.1387016/1‑150 (MQ=60)
                                                                                                                TGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACC                                       >  SRR19973065.1414201/1‑150 (MQ=60)
                                                                                                                  TTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTG                                     >  SRR19973065.2178449/1‑150 (MQ=60)
                                                                                                                    CGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCC                                   <  SRR19973065.3266851/150‑1 (MQ=60)
                                                                                                                    CGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCC                                   <  SRR19973065.3266869/150‑1 (MQ=60)
                                                                                                                     GATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCG                                  <  SRR19973065.3122338/150‑1 (MQ=60)
                                                                                                                            TGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTC                           >  SRR19973065.1398498/1‑150 (MQ=60)
                                                                                                                             GCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCT                          >  SRR19973065.832639/1‑150 (MQ=60)
                                                                                                                             GCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCT                          <  SRR19973065.280271/150‑1 (MQ=60)
                                                                                                                              CCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCAGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTG                         <  SRR19973065.1190599/150‑1 (MQ=60)
                                                                                                                                GTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGT                       <  SRR19973065.3102426/150‑1 (MQ=60)
                                                                                                                                GTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGT                       <  SRR19973065.2876956/150‑1 (MQ=60)
                                                                                                                                    CGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCG                   >  SRR19973065.2638771/1‑150 (MQ=60)
                                                                                                                                         CGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGG              >  SRR19973065.3428918/1‑150 (MQ=60)
                                                                                                                                          GACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGG             >  SRR19973065.889264/1‑150 (MQ=60)
                                                                                                                                          GACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGG             >  SRR19973065.2656704/1‑150 (MQ=60)
                                                                                                                                             GCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCG          >  SRR19973065.2930379/1‑150 (MQ=60)
                                                                                                                                              CGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGA         <  SRR19973065.293702/150‑1 (MQ=60)
                                                                                                                                              CGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGA         <  SRR19973065.292799/150‑1 (MQ=60)
                                                                                                                                              CGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGA         <  SRR19973065.1874580/150‑1 (MQ=60)
                                                                                                                                               GGCGAGAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGGGCCGGGCGGGGCGAC        <  SRR19973065.2005606/150‑1 (MQ=60)
                                                                                                                                                  GATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCT     <  SRR19973065.1212286/150‑1 (MQ=60)
                                                                                                                                                  GATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCT     >  SRR19973065.1564123/1‑150 (MQ=60)
                                                                                                                                                  GATAGTGTTTCGCCGCCTGATCCGCTCCCAGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCCTGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCT     <  SRR19973065.1211765/150‑1 (MQ=60)
                                                                                                                                                   ATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTG    >  SRR19973065.822142/1‑150 (MQ=60)
                                                                                                                                                     AGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCC  <  SRR19973065.1131154/150‑1 (MQ=60)
                                                                                                                                                     AGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCC  >  SRR19973065.1111090/1‑150 (MQ=60)
                                                                                                                                                     AGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCC  >  SRR19973065.1111037/1‑150 (MQ=60)
                                                                                                                                                     AGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCC  <  SRR19973065.1781773/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ATCGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCC  >  CP009273/279185‑279483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑