Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 992,767 C→A 100% D68Y (GAT→TAT)  ssuE ← NAD(P)H‑dependent FMN reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273992,7670CA97.2% 651.8 / 7.1 213D68Y (GAT→TAT) ssuENAD(P)H‑dependent FMN reductase
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (85/122);  total (88/125)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 213 total aligned reads displayed.

GCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCA  >  CP009273/992622‑992910
                                                                                                                                                 |                                                                                                                                               
gCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAgg                                                                                                                                               >  2:2346000/1‑148 (MQ=255)
 ccAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGc                                                                                                                                              <  2:1283538/148‑1 (MQ=255)
    gcagATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTg                                                                                                                                           >  2:1997317/1‑148 (MQ=255)
    gcagATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTg                                                                                                                                           >  2:2542891/1‑148 (MQ=255)
    gcagATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTg                                                                                                                                           <  2:1231870/148‑1 (MQ=255)
     cagATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGt                                                                                                                                          <  1:2004593/148‑1 (MQ=255)
     cagATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGt                                                                                                                                          <  2:509334/148‑1 (MQ=255)
             ccACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTg                                                                                                                                  >  1:2654511/1‑148 (MQ=255)
             ccACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTg                                                                                                                                  <  2:2591566/148‑1 (MQ=255)
                    aCCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTg                                                                                                                           <  1:2264646/148‑1 (MQ=255)
                     ccGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGa                                                                                                                          <  2:1222356/148‑1 (MQ=255)
                      cGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGACTAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGaa                                                                                                                         >  2:645648/1‑148 (MQ=255)
                       gCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAg                                                                                                                        <  2:1626681/148‑1 (MQ=255)
                         ccGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCANCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGt                                                                                                                      <  1:1780287/148‑1 (MQ=255)
                         ccGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGt                                                                                                                      <  1:1964909/148‑1 (MQ=255)
                          cGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTc                                                                                                                     <  1:2186132/148‑1 (MQ=255)
                           gTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCAGAGGCCTGTTGCAGCTGTTCGGTGAAGGTCt                                                                                                                    >  1:3425477/1‑148 (MQ=255)
                             cGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTg                                                                                                                  <  1:1691865/148‑1 (MQ=255)
                               ccAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTACAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAg                                                                                                                <  1:3403725/148‑1 (MQ=255)
                                  gCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGc                                                                                                             >  1:598993/1‑148 (MQ=255)
                                      tAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGa                                                                                                         <  1:892713/148‑1 (MQ=255)
                                       aGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGAc                                                                                                        <  2:2284691/148‑1 (MQ=255)
                                       aGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGAc                                                                                                        >  1:767786/1‑148 (MQ=255)
                                        gCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACt                                                                                                       >  1:3236568/1‑148 (MQ=255)
                                         caccacTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTa                                                                                                      <  2:767255/148‑1 (MQ=255)
                                         caccacTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTa                                                                                                      >  2:847649/1‑148 (MQ=255)
                                             acTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGa                                                                                                  <  2:3397900/148‑1 (MQ=255)
                                                 tGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACg                                                                                              <  2:2235601/148‑1 (MQ=255)
                                                 tGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACg                                                                                              <  1:1225511/148‑1 (MQ=255)
                                                   ccTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAg                                                                                            <  2:1366449/148‑1 (MQ=255)
                                                    cTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGc                                                                                           <  1:2412286/148‑1 (MQ=255)
                                                     ttGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCa                                                                                          <  1:2630037/148‑1 (MQ=255)
                                                        cAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATaa                                                                                       <  1:622727/148‑1 (MQ=255)
                                                         aaaGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATaaa                                                                                      <  2:2858496/148‑1 (MQ=255)
                                                          aaGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAg                                                                                     >  2:188790/1‑148 (MQ=255)
                                                            gcgcGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTa                                                                                   <  2:2799297/148‑1 (MQ=255)
                                                              gcgTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGa                                                                                 <  2:1517533/148‑1 (MQ=255)
                                                               cgTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGAt                                                                                >  1:165735/1‑148 (MQ=255)
                                                               cgTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGAt                                                                                >  2:1590958/1‑148 (MQ=255)
                                                                  tCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCtt                                                                             >  2:1553349/1‑148 (MQ=255)
                                                                  tCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCtt                                                                             <  2:3116038/148‑1 (MQ=255)
                                                                   cTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTc                                                                            <  1:1033705/148‑1 (MQ=255)
                                                                     ggCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCg                                                                          <  1:3502320/148‑1 (MQ=255)
                                                                         gcagGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGc                                                                      >  2:597470/1‑148 (MQ=255)
                                                                            ggTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGaa                                                                   >  2:783367/1‑148 (MQ=255)
                                                                             gTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAg                                                                  <  1:1852833/148‑1 (MQ=255)
                                                                             gTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAg                                                                  <  1:2321890/148‑1 (MQ=255)
                                                                              tCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGt                                                                 <  2:147342/148‑1 (MQ=255)
                                                                                 aGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGtttt                                                              >  2:1541101/1‑148 (MQ=255)
                                                                                  gCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTg                                                             <  2:3242466/148‑1 (MQ=255)
                                                                                   cAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGc                                                            >  2:398381/1‑148 (MQ=255)
                                                                                     gggTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAg                                                          >  2:1857628/1‑148 (MQ=255)
                                                                                       gTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGAt                                                        >  2:3085033/1‑148 (MQ=255)
                                                                                        ttttCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGACACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGAtt                                                       >  1:3302292/1‑148 (MQ=255)
                                                                                        ttttCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGAtt                                                       <  1:3151946/148‑1 (MQ=255)
                                                                                             aaCGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGt                                                  <  1:479784/148‑1 (MQ=255)
                                                                                              aCGCACCGGAATAGGCGGCTTTATACACCGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTg                                                 <  2:2044379/148‑1 (MQ=255)
                                                                                                gCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGAt                                               >  1:2700847/1‑148 (MQ=255)
                                                                                                   ccGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATaaa                                            >  1:745618/1‑148 (MQ=255)
                                                                                                    cGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAc                                           >  2:1672619/1‑148 (MQ=255)
                                                                                                    cGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGACGTTTTGCAGATTCCAGTGATAAAc                                           >  1:710269/1‑148 (MQ=255)
                                                                                                    cGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAc                                           >  1:2084583/1‑148 (MQ=255)
                                                                                                    cGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTCCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAc                                           >  1:2443458/1‑148 (MQ=255)
                                                                                                     ggAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAcc                                          >  2:1844884/1‑148 (MQ=255)
                                                                                                     ggAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAcc                                          <  2:3445878/148‑1 (MQ=255)
                                                                                                      taaTAGGCGGCTTTATACTCAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTATTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACct                                         <  2:1894367/147‑1 (MQ=255)
                                                                                                      gAATAGGCGGCTTTATACCCAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACct                                         <  2:1655124/148‑1 (MQ=255)
                                                                                                      gAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACct                                         <  2:1895519/148‑1 (MQ=255)
                                                                                                      gAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACct                                         <  1:438223/148‑1 (MQ=255)
                                                                                                       aaTAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACctc                                        <  2:2386227/148‑1 (MQ=255)
                                                                                                        aTAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACctct                                       <  2:2654506/148‑1 (MQ=255)
                                                                                                            gcggcTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACAt                                   >  1:3012825/1‑148 (MQ=255)
                                                                                                             cggcTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATc                                  <  2:3350688/148‑1 (MQ=255)
                                                                                                              ggcTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATcc                                 <  2:1798049/148‑1 (MQ=255)
                                                                                                               gcTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCa                                >  2:3421342/1‑148 (MQ=255)
                                                                                                                cTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAg                               >  1:351473/1‑148 (MQ=255)
                                                                                                                cTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAg                               >  1:2120898/1‑148 (MQ=255)
                                                                                                                  ttATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGc                             >  1:1648506/1‑148 (MQ=255)
                                                                                                                  ttATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGc                             >  2:3015065/1‑148 (MQ=255)
                                                                                                                   tataCACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCTAGTGATAAACCTCTACATCCAGGcc                            <  1:647060/148‑1 (MQ=255)
                                                                                                                   tataCACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGcc                            <  1:2533062/148‑1 (MQ=255)
                                                                                                                    ataCACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCa                           <  1:509412/148‑1 (MQ=255)
                                                                                                                     taCACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCAt                          >  2:66766/1‑148 (MQ=255)
                                                                                                                      acacAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCAtt                         >  2:420625/1‑148 (MQ=255)
                                                                                                                       cacaGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCAttt                        <  1:1837788/148‑1 (MQ=255)
                                                                                                                       cacaGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCAttt                        <  2:2426465/148‑1 (MQ=255)
                                                                                                                         caGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAg                      <  1:188850/148‑1 (MQ=255)
                                                                                                                         caGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAg                      <  1:2918221/148‑1 (MQ=255)
                                                                                                                          aGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGt                     <  1:2348873/148‑1 (MQ=255)
                                                                                                                                ggCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTcc               <  1:1541101/148‑1 (MQ=255)
                                                                                                                                 gCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTccc              >  1:1461686/1‑148 (MQ=255)
                                                                                                                                  cGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCcg             >  1:1543422/1‑148 (MQ=255)
                                                                                                                                  cGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCcg             >  2:2666870/1‑148 (MQ=255)
                                                                                                                                     cAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCgcgc          >  1:2596408/1‑148 (MQ=255)
                                                                                                                                       aTCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCat        >  2:1324882/1‑148 (MQ=255)
                                                                                                                                        tCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCata       >  1:1496473/1‑148 (MQ=255)
                                                                                                                                          aGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATAtt     <  1:1553349/148‑1 (MQ=255)
                                                                                                                                            cccATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTcc   <  2:1130145/148‑1 (MQ=255)
                                                                                                                                            cccATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTcc   >  2:886604/1‑148 (MQ=255)
                                                                                                                                             ccATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCa  >  2:1742025/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCA  >  CP009273/992622‑992910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGG  >  CP009273/992619‑992916
                                                                                                                                                    |                                                                                                                                                     
ACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAG                                                                                                                                                      >  SRR19973065.2666016/1‑150 (MQ=60)
 CCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGG                                                                                                                                                     <  SRR19973065.2378141/150‑1 (MQ=60)
       GCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTT                                                                                                                                               <  SRR19973065.2488849/150‑1 (MQ=60)
        CAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTG                                                                                                                                              <  SRR19973065.2017549/150‑1 (MQ=60)
            TGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGC                                                                                                                                          <  SRR19973065.370626/150‑1 (MQ=60)
             GGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCT                                                                                                                                         <  SRR19973065.2151814/150‑1 (MQ=60)
             GGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCT                                                                                                                                         <  SRR19973065.1544258/150‑1 (MQ=60)
              GGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTG                                                                                                                                        >  SRR19973065.2052308/1‑150 (MQ=60)
              GGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTG                                                                                                                                        >  SRR19973065.2670904/1‑150 (MQ=60)
              GGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTG                                                                                                                                        >  SRR19973065.2670909/1‑150 (MQ=60)
                CCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTT                                                                                                                                      <  SRR19973065.72039/150‑1 (MQ=60)
                CCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTT                                                                                                                                      <  SRR19973065.1862146/150‑1 (MQ=60)
                       ACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAA                                                                                                                               <  SRR19973065.2278962/150‑1 (MQ=60)
                            CCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCANCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCT                                                                                                                          <  SRR19973065.1791911/150‑1 (MQ=60)
                            CCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACCATCAGCCCAGAGGCGTGTTGCAGCTGTTCGGTGAAGGTCT                                                                                                                          >  SRR19973065.3446383/1‑150 (MQ=60)
                            CCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCT                                                                                                                          <  SRR19973065.1977736/150‑1 (MQ=60)
                             CGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTT                                                                                                                         <  SRR19973065.2200155/150‑1 (MQ=60)
                              GTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTG                                                                                                                        <  SRR19973065.1913395/150‑1 (MQ=60)
                                CGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAG                                                                                                                      <  SRR19973065.1703161/150‑1 (MQ=60)
                                  CCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTACAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTG                                                                                                                    <  SRR19973065.3424525/150‑1 (MQ=60)
                                   CAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGC                                                                                                                   >  SRR19973065.602727/1‑150 (MQ=60)
                                   CAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGC                                                                                                                   >  SRR19973065.602127/1‑150 (MQ=60)
                                   CAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGC                                                                                                                   >  SRR19973065.2401193/1‑150 (MQ=60)
                                        GTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGAC                                                                                                              >  SRR19973065.772887/1‑150 (MQ=60)
                                         TAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACT                                                                                                             <  SRR19973065.898385/150‑1 (MQ=60)
                                         TAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACT                                                                                                             >  SRR19973065.3256050/1‑150 (MQ=60)
                                          AGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTA                                                                                                            <  SRR19973065.1689381/150‑1 (MQ=60)
                                            CACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATC                                                                                                          <  SRR19973065.512197/150‑1 (MQ=60)
                                                 CTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAAC                                                                                                     <  SRR19973065.660301/150‑1 (MQ=60)
                                                    TGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAG                                                                                                  <  SRR19973065.1233502/150‑1 (MQ=60)
                                                       CTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCAT                                                                                               <  SRR19973065.2427475/150‑1 (MQ=60)
                                                        TTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATA                                                                                              <  SRR19973065.2646300/150‑1 (MQ=60)
                                                           CAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAG                                                                                           <  SRR19973065.626751/150‑1 (MQ=60)
                                                           CAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAG                                                                                           <  SRR19973065.2508292/150‑1 (MQ=60)
                                                            AAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGT                                                                                          <  SRR19973065.26340/150‑1 (MQ=60)
                                                                CGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGAT                                                                                      >  SRR19973065.1807705/1‑150 (MQ=60)
                                                                CGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGAT                                                                                      >  SRR19973065.167089/1‑150 (MQ=60)
                                                                CGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGAT                                                                                      >  SRR19973065.167080/1‑150 (MQ=60)
                                                                  CGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCT                                                                                    <  SRR19973065.467119/150‑1 (MQ=60)
                                                                      CTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCG                                                                                <  SRR19973065.1040548/150‑1 (MQ=60)
                                                                        GGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGG                                                                              <  SRR19973065.3523588/150‑1 (MQ=60)
                                                                        GGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGG                                                                              <  SRR19973065.3523639/150‑1 (MQ=60)
                                                                         GCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGGGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGG                                                                             <  SRR19973065.1611160/150‑1 (MQ=60)
                                                                                GTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTT                                                                      <  SRR19973065.3510588/150‑1 (MQ=60)
                                                                                GTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTT                                                                      <  SRR19973065.1865036/150‑1 (MQ=60)
                                                                                GTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTT                                                                      <  SRR19973065.2336439/150‑1 (MQ=60)
                                                                                         ggtgttgttCGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATT                                                             <  SRR19973065.2010261/141‑1 (MQ=60)
                                                                                         GGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGACACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATT                                                             >  SRR19973065.3322402/1‑150 (MQ=60)
                                                                                           TTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCC                                                           <  SRR19973065.3171071/150‑1 (MQ=60)
                                                                                              TCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGT                                                        <  SRR19973065.2738076/150‑1 (MQ=60)
                                                                                               CAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTG                                                       >  SRR19973065.611436/1‑150 (MQ=60)
                                                                                                AACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGA                                                      <  SRR19973065.483050/150‑1 (MQ=60)
                                                                                                AACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGA                                                      <  SRR19973065.483051/150‑1 (MQ=60)
                                                                                                 ACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGAT                                                     >  SRR19973065.2717627/1‑150 (MQ=60)
                                                                                                 ACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGAT                                                     <  SRR19973065.2706353/150‑1 (MQ=60)
                                                                                                   GCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAA                                                   <  SRR19973065.871339/150‑1 (MQ=60)
                                                                                                    CACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAA                                                  >  SRR19973065.750634/1‑150 (MQ=60)
                                                                                                    CACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAA                                                  >  SRR19973065.750606/1‑150 (MQ=60)
                                                                                                     ACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGACGTTTTGCAGATTCCAGTGATAAAC                                                 >  SRR19973065.715016/1‑150 (MQ=60)
                                                                                                     ACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAC                                                 >  SRR19973065.2097947/1‑150 (MQ=60)
                                                                                                     ACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAC                                                 >  SRR19973065.2458362/1‑150 (MQ=60)
                                                                                                     ACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAC                                                 >  SRR19973065.3063005/1‑150 (MQ=60)
                                                                                                     ACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAC                                                 >  SRR19973065.3063002/1‑150 (MQ=60)
                                                                                                     ACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTCCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAC                                                 >  SRR19973065.2458857/1‑150 (MQ=60)
                                                                                                         GAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCT                                             <  SRR19973065.441261/150‑1 (MQ=60)
                                                                                                          AATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTA                                            <  SRR19973065.3242870/150‑1 (MQ=60)
                                                                                                             AGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACAT                                         >  SRR19973065.3031275/1‑150 (MQ=60)
                                                                                                                CGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCA                                      <  SRR19973065.1889767/150‑1 (MQ=60)
                                                                                                                CGGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCA                                      >  SRR19973065.1502358/1‑150 (MQ=60)
                                                                                                                 GGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAG                                     >  SRR19973065.353938/1‑150 (MQ=60)
                                                                                                                 GGCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAG                                     >  SRR19973065.2134551/1‑150 (MQ=60)
                                                                                                                  GCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGG                                    >  SRR19973065.1757168/1‑150 (MQ=60)
                                                                                                                  GCTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGG                                    >  SRR19973065.300947/1‑150 (MQ=60)
                                                                                                                   CTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGC                                   >  SRR19973065.1659566/1‑150 (MQ=60)
                                                                                                                   CTTTATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGC                                   >  SRR19973065.1660061/1‑150 (MQ=60)
                                                                                                                      TATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCTAGTGATAAACCTCTACATCCAGGCCAT                                <  SRR19973065.651315/150‑1 (MQ=60)
                                                                                                                      TATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCAT                                <  SRR19973065.2548962/150‑1 (MQ=60)
                                                                                                                      TATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCAT                                <  SRR19973065.649894/150‑1 (MQ=60)
                                                                                                                       ATACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATT                               <  SRR19973065.512794/150‑1 (MQ=60)
                                                                                                                        TACTCTGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCTGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTT                              <  SRR19973065.2569994/150‑1 (MQ=60)
                                                                                                                        TACACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTT                              <  SRR19973065.1184156/150‑1 (MQ=60)
                                                                                                                          CACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAG                            <  SRR19973065.1849907/150‑1 (MQ=60)
                                                                                                                          CACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAG                            <  SRR19973065.2025550/150‑1 (MQ=60)
                                                                                                                          CACAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAG                            >  SRR19973065.2282697/1‑150 (MQ=60)
                                                                                                                            CAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTT                          <  SRR19973065.2935966/150‑1 (MQ=60)
                                                                                                                            CAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTT                          <  SRR19973065.190326/150‑1 (MQ=60)
                                                                                                                            CAGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTT                          <  SRR19973065.1350494/150‑1 (MQ=60)
                                                                                                                             AGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTT                         >  SRR19973065.2100138/1‑150 (MQ=60)
                                                                                                                             AGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTT                         <  SRR19973065.2363428/150‑1 (MQ=60)
                                                                                                                             AGGCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTT                         <  SRR19973065.2363509/150‑1 (MQ=60)
                                                                                                                               GCGTGGCGACAATCAGCCCATAGGCCTGTTGCAGATGTTCGGTTAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCCGTGATAAACCTCTACATCCAGGCCATTTAGTTTTT                       <  SRR19973065.2011826/150‑1 (MQ=60)
                                                                                                                                 GTGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCC                     <  SRR19973065.3442240/150‑1 (MQ=60)
                                                                                                                                  TGGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCC                    >  SRR19973065.1471080/1‑150 (MQ=60)
                                                                                                                                   GGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCG                   <  SRR19973065.1551287/150‑1 (MQ=60)
                                                                                                                                   GGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCG                   >  SRR19973065.1553395/1‑150 (MQ=60)
                                                                                                                                   GGCGACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCG                   >  SRR19973065.1553631/1‑150 (MQ=60)
                                                                                                                                      GACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGC                >  SRR19973065.843338/1‑150 (MQ=60)
                                                                                                                                      GACAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCACGCGC                >  SRR19973065.2612546/1‑150 (MQ=60)
                                                                                                                                        CAATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCAT              >  SRR19973065.3265019/1‑150 (MQ=60)
                                                                                                                                         AATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATA             >  SRR19973065.1506101/1‑150 (MQ=60)
                                                                                                                                         AATCAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATA             <  SRR19973065.226641/150‑1 (MQ=60)
                                                                                                                                            CAGCCCATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTC          >  SRR19973065.3387252/1‑150 (MQ=60)
                                                                                                                                             AGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCC         <  SRR19973065.1563645/150‑1 (MQ=60)
                                                                                                                                             AGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCC         <  SRR19973065.1563648/150‑1 (MQ=60)
                                                                                                                                                 CATAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCA     >  SRR19973065.1205914/1‑150 (MQ=60)
                                                                                                                                                   TAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAG   >  SRR19973065.2716082/1‑150 (MQ=60)
                                                                                                                                                   TAGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAG   <  SRR19973065.853143/150‑1 (MQ=60)
                                                                                                                                                    AGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGG  >  SRR19973065.242310/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGG  >  CP009273/992619‑992916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑