Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,514,177 G→T 100% E298D (GAG→GAT)  curA → curcumin/dihydrocurcumin reductase, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,514,1770GT96.4% 568.4 / 11.5 193E298D (GAG→GAT) curAcurcumin/dihydrocurcumin reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (4/3);  new base T (96/90);  total (100/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 196 total aligned reads displayed.

TGAGCAGCTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAA  >  CP009273/1514032‑1514321
                                                                                                                                                 |                                                                                                                                                
tGAGCAGCTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGatat                                                                                                                                                <  1:300279/148‑1 (MQ=255)
tGAGCAGCTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGatat                                                                                                                                                <  1:2510921/148‑1 (MQ=255)
tGAGCAGCTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCACATCCATGAGTTTCAGAGGGatat                                                                                                                                                >  1:1465995/1‑148 (MQ=255)
      gcTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCa                                                                                                                                          <  2:2604514/148‑1 (MQ=255)
       cTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCaa                                                                                                                                         <  1:886301/148‑1 (MQ=255)
        tataACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAAt                                                                                                                                        <  1:3066834/148‑1 (MQ=255)
          taACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATgg                                                                                                                                      >  1:2222541/1‑148 (MQ=255)
           aaCGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATggg                                                                                                                                     >  1:1126615/1‑148 (MQ=255)
           aaCGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATggg                                                                                                                                     >  1:2603233/1‑148 (MQ=255)
           aaCGCTACAGAGCTACCACCCGGTCCGGATCGTGTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATggg                                                                                                                                     <  1:802798/148‑1 (MQ=255)
               cTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGaa                                                                                                                                 <  2:2128081/148‑1 (MQ=255)
               cTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTACTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGaa                                                                                                                                 <  1:2912419/148‑1 (MQ=255)
                 aCAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAag                                                                                                                               <  2:3044469/148‑1 (MQ=255)
                  cAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAaga                                                                                                                              <  1:3347178/148‑1 (MQ=255)
                  cAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAaga                                                                                                                              <  1:548234/148‑1 (MQ=255)
                    gagCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAgg                                                                                                                            <  1:270976/148‑1 (MQ=255)
                     agCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGa                                                                                                                           <  2:2011480/148‑1 (MQ=255)
                     agCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGa                                                                                                                           >  1:498452/1‑148 (MQ=255)
                      gCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGAt                                                                                                                          <  2:2261565/148‑1 (MQ=255)
                        tACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATaa                                                                                                                        <  2:581795/148‑1 (MQ=255)
                            accCTGTCCGTATCTTTTACCTCTGTTGATGGCTACAGTGCTGAAACAACGTATTCGCTTGCAATGTTTTATTATCGCTCTGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATc                                                                                                                    <  2:3285459/148‑1 (MQ=255)
                             cccGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATcc                                                                                                                   >  2:683408/1‑148 (MQ=255)
                              ccGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCa                                                                                                                  <  2:162216/148‑1 (MQ=255)
                              ccGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCa                                                                                                                  <  1:2819189/148‑1 (MQ=255)
                                ggTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACt                                                                                                                <  2:3503340/148‑1 (MQ=255)
                                 gTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTa                                                                                                               <  1:1879491/148‑1 (MQ=255)
                                   ccGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTAcc                                                                                                             >  1:3481358/1‑148 (MQ=255)
                                      gATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAATACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACcgcg                                                                                                          >  2:105795/1‑148 (MQ=255)
                                       aTCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCga                                                                                                         <  2:30331/148‑1 (MQ=255)
                                        tCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCgaa                                                                                                        >  1:2997580/1‑148 (MQ=255)
                                            ttACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGaaa                                                                                                    <  2:1617860/148‑1 (MQ=255)
                                            ttACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGaaa                                                                                                    >  1:2652238/1‑148 (MQ=255)
                                              aCCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAAtt                                                                                                  <  2:656802/148‑1 (MQ=255)
                                              aCCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAAtt                                                                                                  <  2:2664759/148‑1 (MQ=255)
                                              aCCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAAtt                                                                                                  <  2:686536/148‑1 (MQ=255)
                                               ccTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTa                                                                                                 >  2:377135/1‑148 (MQ=255)
                                               ccTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTa                                                                                                 <  1:816937/148‑1 (MQ=255)
                                               ccTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTa                                                                                                 <  1:2585044/148‑1 (MQ=255)
                                                   tGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGa                                                                                             >  2:2937222/1‑148 (MQ=255)
                                                    gTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGAc                                                                                            >  2:124637/1‑148 (MQ=255)
                                                     ttGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACg                                                                                           >  1:2029811/1‑148 (MQ=255)
                                                      tGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACgg                                                                                          <  2:3141652/148‑1 (MQ=255)
                                                        aTGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGtt                                                                                        >  1:1520770/1‑148 (MQ=255)
                                                        aTGGCTAAAGTGCTTAAAAAAAGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTAACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGTTGATAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGtt                                                                                        <  2:744315/148‑1 (MQ=255)
                                                         tGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGttt                                                                                       >  1:1353695/1‑148 (MQ=255)
                                                              aCAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAgaga                                                                                  >  1:3288028/1‑148 (MQ=255)
                                                               cAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGaa                                                                                 <  2:2579480/148‑1 (MQ=255)
                                                                 gTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATg                                                                               <  2:486950/148‑1 (MQ=255)
                                                                  tGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATgc                                                                              >  1:2225244/1‑148 (MQ=255)
                                                                  tGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATgc                                                                              >  2:203658/1‑148 (MQ=255)
                                                                  tGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGGTTAGAGAATgc                                                                              >  2:458750/1‑148 (MQ=255)
                                                                    cTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATgcgc                                                                            <  1:1308403/148‑1 (MQ=255)
                                                                     tGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGcc                                                                           >  2:95135/1‑148 (MQ=255)
                                                                       aaaaaaCGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCcac                                                                         >  1:1043587/1‑148 (MQ=255)
                                                                           aaCGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGAc                                                                     >  1:1446050/1‑148 (MQ=255)
                                                                             cGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGt                                                                   >  1:2072642/1‑148 (MQ=255)
                                                                               tATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGttt                                                                 >  2:1907881/1‑148 (MQ=255)
                                                                                aTTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTa                                                                >  2:2042630/1‑148 (MQ=255)
                                                                                  tCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCCATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGACATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATc                                                              >  2:1786339/1‑148 (MQ=255)
                                                                                   cGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCg                                                             >  1:632274/1‑148 (MQ=255)
                                                                                    gCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCgg                                                            >  2:1487859/1‑148 (MQ=255)
                                                                                    gCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATAgg                                                            >  2:1487910/1‑148 (MQ=255)
                                                                                     cTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGc                                                           <  2:3482982/148‑1 (MQ=255)
                                                                                     cTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGc                                                           <  1:629182/148‑1 (MQ=255)
                                                                                      ttGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGcc                                                          <  2:1267794/148‑1 (MQ=255)
                                                                                        gCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCctg                                                        <  1:3405753/148‑1 (MQ=255)
                                                                                         cAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCctgc                                                       >  2:22007/1‑148 (MQ=255)
                                                                                         cAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCctgc                                                       >  1:566698/1‑148 (MQ=255)
                                                                                         cAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCctgc                                                       >  2:2110246/1‑148 (MQ=255)
                                                                                             gTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCACTGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGaa                                                   >  2:2165877/1‑148 (MQ=255)
                                                                                             gTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGaa                                                   >  2:232712/1‑148 (MQ=255)
                                                                                               tttATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAgg                                                 <  1:447352/148‑1 (MQ=255)
                                                                                               tttATGATCGCTCAGGATTATGGACACCGCATCCATGAGTTACAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAgg                                                 <  2:1984005/148‑1 (MQ=255)
                                                                                                   ttatCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTaa                                             >  2:2542883/1‑148 (MQ=255)
                                                                                                      tCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTcaaaa                                          <  2:2711480/148‑1 (MQ=255)
                                                                                                        gCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACt                                        <  2:1098595/148‑1 (MQ=255)
                                                                                                         cTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACtt                                       <  1:2546454/148‑1 (MQ=255)
                                                                                                           cAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCg                                     >  2:1551410/1‑148 (MQ=255)
                                                                                                           cAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCg                                     >  2:2433434/1‑148 (MQ=255)
                                                                                                               aTTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCaa                                 >  1:2744129/1‑148 (MQ=255)
                                                                                                                ttATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCaaa                                <  1:683408/148‑1 (MQ=255)
                                                                                                                 tATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAg                               >  1:1693255/1‑148 (MQ=255)
                                                                                                                    ggTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAgtgg                            >  2:2591417/1‑148 (MQ=255)
                                                                                                                     gTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAgtggt                           <  2:1133284/148‑1 (MQ=255)
                                                                                                                       cACCGCATCCATGAGTTTCAGAGTGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGa                         >  1:2604982/1‑148 (MQ=255)
                                                                                                                       cACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGa                         <  2:3212892/148‑1 (MQ=255)
                                                                                                                        aCCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGAt                        >  2:845738/1‑148 (MQ=255)
                                                                                                                            cATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCcgc                    <  2:193437/148‑1 (MQ=255)
                                                                                                                              tCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGt                  >  1:1966216/1‑148 (MQ=255)
                                                                                                                               ccATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTg                 >  2:1711566/1‑148 (MQ=255)
                                                                                                                               ccATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTg                 >  1:1309902/1‑148 (MQ=255)
                                                                                                                                cATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTgg                <  2:2489785/148‑1 (MQ=255)
                                                                                                                                      tttCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGtg          >  1:2155499/1‑148 (MQ=255)
                                                                                                                                         cAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGtgatg       <  1:2937222/148‑1 (MQ=255)
                                                                                                                                          agagGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGtgatga      <  1:124637/148‑1 (MQ=255)
                                                                                                                                           gagGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTgatgat     <  2:2997580/148‑1 (MQ=255)
                                                                                                                                           gagGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTgatgat     >  2:1813796/1‑148 (MQ=255)
                                                                                                                                           gagGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTgatgat     >  2:842608/1‑148 (MQ=255)
                                                                                                                                            agGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGAtt    >  2:2416653/1‑148 (MQ=255)
                                                                                                                                              ggATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTaa  >  2:2918700/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGAGCAGCTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAA  >  CP009273/1514032‑1514321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGTGAGCAGCTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTT  >  CP009273/1514029‑1514325
                                                                                                                                                    |                                                                                                                                                    
TAGTGAGCAGCTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATA                                                                                                                                                     >  SRR19973065.1276155/1‑150 (MQ=60)
TAGTGAGCAGCTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATA                                                                                                                                                     >  SRR19973065.1276158/1‑150 (MQ=60)
 AGTGAGCAGCTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCACATCCATGAGTTTCAGAGGGATAT                                                                                                                                                    >  SRR19973065.1475401/1‑150 (MQ=60)
  GTGAGCAGCTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATG                                                                                                                                                   <  SRR19973065.2687407/150‑1 (MQ=60)
   TGAGCAGCTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGG                                                                                                                                                  <  SRR19973065.302229/150‑1 (MQ=60)
   TGAGCAGCTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGG                                                                                                                                                  <  SRR19973065.2526700/150‑1 (MQ=60)
       CAGCTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGTGATATGGGGCA                                                                                                                                              >  SRR19973065.2619543/1‑150 (MQ=60)
          CTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATG                                                                                                                                           <  SRR19973065.891932/150‑1 (MQ=60)
          CTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATG                                                                                                                                           >  SRR19973065.2968283/1‑150 (MQ=60)
          CTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATG                                                                                                                                           <  SRR19973065.2659052/150‑1 (MQ=60)
           TATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGG                                                                                                                                          <  SRR19973065.3085579/150‑1 (MQ=60)
           TATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGG                                                                                                                                          >  SRR19973065.2236721/1‑150 (MQ=60)
            ATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGG                                                                                                                                         >  SRR19973065.1133944/1‑150 (MQ=60)
            ATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGG                                                                                                                                         >  SRR19973065.2619397/1‑150 (MQ=60)
             TAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGT                                                                                                                                        <  SRR19973065.2675664/150‑1 (MQ=60)
              AACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTG                                                                                                                                       <  SRR19973065.478131/150‑1 (MQ=60)
              AACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTG                                                                                                                                       <  SRR19973065.808121/150‑1 (MQ=60)
              AACGCTACAGAGCTACCACCCGGTCCGGATCGTGTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTG                                                                                                                                       <  SRR19973065.808104/150‑1 (MQ=60)
                CGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCACTGGGTGAA                                                                                                                                     >  SRR19973065.167298/1‑150 (MQ=60)
                CGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAA                                                                                                                                     >  SRR19973065.1138108/1‑150 (MQ=60)
                  CTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTACTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAG                                                                                                                                   <  SRR19973065.2930143/150‑1 (MQ=60)
                     CAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTTCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGG                                                                                                                                <  SRR19973065.3367627/150‑1 (MQ=60)
                     CAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGG                                                                                                                                <  SRR19973065.551765/150‑1 (MQ=60)
                      AGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGA                                                                                                                               >  SRR19973065.501797/1‑150 (MQ=60)
                      AGAGCTACCACCCGGTCAGGATCGTTTACCTCTGTTGATTGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGTGAAATTGGTGAAAGAGGA                                                                                                                               >  SRR19973065.501977/1‑150 (MQ=60)
                       GAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGAT                                                                                                                              <  SRR19973065.272808/150‑1 (MQ=60)
                       GAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGAT                                                                                                                              <  SRR19973065.210052/150‑1 (MQ=60)
                                 CCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACT                                                                                                                    <  SRR19973065.2836605/150‑1 (MQ=60)
                                   GGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTAC                                                                                                                  >  SRR19973065.3425544/1‑150 (MQ=60)
                                   GGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTAC                                                                                                                  >  SRR19973065.671595/1‑150 (MQ=60)
                                    GTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACC                                                                                                                 >  SRR19973065.3502542/1‑150 (MQ=60)
                                    GTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACC                                                                                                                 <  SRR19973065.2946912/150‑1 (MQ=60)
                                    GTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACC                                                                                                                 <  SRR19973065.1891863/150‑1 (MQ=60)
                                    GTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACC                                                                                                                 >  SRR19973065.3502463/1‑150 (MQ=60)
                                         GATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAA                                                                                                            >  SRR19973065.3016051/1‑150 (MQ=60)
                                         GATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAA                                                                                                            >  SRR19973065.3015941/1‑150 (MQ=60)
                                            CGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAA                                                                                                         <  SRR19973065.2606187/150‑1 (MQ=60)
                                             GTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAA                                                                                                        >  SRR19973065.2668607/1‑150 (MQ=60)
                                             GTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAA                                                                                                        >  SRR19973065.633480/1‑150 (MQ=60)
                                              TTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAAT                                                                                                       <  SRR19973065.697621/150‑1 (MQ=60)
                                                 ACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTAC                                                                                                    <  SRR19973065.354164/150‑1 (MQ=60)
                                                  CCTCTTTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACT                                                                                                   <  SRR19973065.822310/150‑1 (MQ=60)
                                                  CCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACT                                                                                                   <  SRR19973065.2601146/150‑1 (MQ=60)
                                                      TGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACG                                                                                               <  SRR19973065.3308665/150‑1 (MQ=60)
                                                      TGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACG                                                                                               >  SRR19973065.2042842/1‑150 (MQ=60)
                                                         TGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTT                                                                                            >  SRR19973065.1530736/1‑150 (MQ=60)
                                                          GATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTT                                                                                           >  SRR19973065.1362663/1‑150 (MQ=60)
                                                            TGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAG                                                                                         <  SRR19973065.3054220/150‑1 (MQ=60)
                                                               CTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGA                                                                                      >  SRR19973065.3308035/1‑150 (MQ=60)
                                                                   AGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGC                                                                                  >  SRR19973065.2239426/1‑150 (MQ=60)
                                                                   AGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGC                                                                                  >  SRR19973065.2239193/1‑150 (MQ=60)
                                                                       CTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCA                                                                              <  SRR19973065.1317037/150‑1 (MQ=60)
                                                                        TGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCAC                                                                             >  SRR19973065.1262323/1‑150 (MQ=60)
                                                                        TGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCAC                                                                             >  SRR19973065.1050485/1‑150 (MQ=60)
                                                                            AAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGAC                                                                         >  SRR19973065.1455401/1‑150 (MQ=60)
                                                                            AAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGAC                                                                         >  SRR19973065.1455546/1‑150 (MQ=60)
                                                                              AACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGT                                                                       >  SRR19973065.2085870/1‑150 (MQ=60)
                                                                              AACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGT                                                                       >  SRR19973065.314300/1‑150 (MQ=60)
                                                                                    TTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCG                                                                 >  SRR19973065.636391/1‑150 (MQ=60)
                                                                                    TTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCG                                                                 <  SRR19973065.2071516/150‑1 (MQ=60)
                                                                                    TTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCG                                                                 <  SRR19973065.875472/150‑1 (MQ=60)
                                                                                        CTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCT                                                             <  SRR19973065.633278/150‑1 (MQ=60)
                                                                                          TGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGC                                                           >  SRR19973065.570289/1‑150 (MQ=60)
                                                                                          TGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGC                                                           >  SRR19973065.570294/1‑150 (MQ=60)
                                                                                           GCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCT                                                          <  SRR19973065.3426562/150‑1 (MQ=60)
                                                                                             AAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGA                                                        >  SRR19973065.902910/1‑150 (MQ=60)
                                                                                              AGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAA                                                       >  SRR19973065.201884/1‑150 (MQ=60)
                                                                                                  TTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGT                                                   <  SRR19973065.450454/150‑1 (MQ=60)
                                                                                                    TATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAA                                                 >  SRR19973065.1940687/1‑150 (MQ=60)
                                                                                                     ATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAA                                                >  SRR19973065.150246/1‑150 (MQ=60)
                                                                                                            CTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCG                                         <  SRR19973065.2562400/150‑1 (MQ=60)
                                                                                                               AGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCA                                      <  SRR19973065.58182/150‑1 (MQ=60)
                                                                                                                GGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAA                                     >  SRR19973065.2761208/1‑150 (MQ=60)
                                                                                                                GGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAA                                     >  SRR19973065.2761200/1‑150 (MQ=60)
                                                                                                                GGATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAA                                     >  SRR19973065.2761196/1‑150 (MQ=60)
                                                                                                                  ATTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAG                                   >  SRR19973065.2169585/1‑150 (MQ=60)
                                                                                                                  ATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAG                                   >  SRR19973065.1704561/1‑150 (MQ=60)
                                                                                                                   TTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGT                                  >  SRR19973065.24897/1‑150 (MQ=60)
                                                                                                                   TTATGGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGT                                  <  SRR19973065.687956/150‑1 (MQ=60)
                                                                                                                       GGTCACCGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTG                              <  SRR19973065.1423180/150‑1 (MQ=60)
                                                                                                                        GTCACCGCATCCATGAGTTTCAGAGTGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGA                             >  SRR19973065.2621152/1‑150 (MQ=60)
                                                                                                                             CGCATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGC                        >  SRR19973065.2580753/1‑150 (MQ=60)
                                                                                                                               CATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGT                      >  SRR19973065.1979048/1‑150 (MQ=60)
                                                                                                                                ATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTG                     >  SRR19973065.1318653/1‑150 (MQ=60)
                                                                                                                                ATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTG                     >  SRR19973065.1318548/1‑150 (MQ=60)
                                                                                                                                ATCCATGAGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTG                     >  SRR19973065.1318534/1‑150 (MQ=60)
                                                                                                                                       AGTTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTG              >  SRR19973065.2169362/1‑150 (MQ=60)
                                                                                                                                         TTTCAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGAT            <  SRR19973065.1751101/150‑1 (MQ=60)
                                                                                                                                            CAGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGAT         <  SRR19973065.2955039/150‑1 (MQ=60)
                                                                                                                                             AGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATT        <  SRR19973065.2533690/150‑1 (MQ=60)
                                                                                                                                             AGAGGGATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATT        <  SRR19973065.125718/150‑1 (MQ=60)
                                                                                                                                                   ATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTT  >  SRR19973065.2868814/1‑150 (MQ=60)
                                                                                                                                                   ATATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTT  >  SRR19973065.2869300/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TAGTGAGCAGCTATAACGCTACAGAGCTACCACCCGGTCCGGATCGTTTACCTCTGTTGATGGCTACAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTT  >  CP009273/1514029‑1514325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑