Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,558,685 G→T 100% R436S (CGC→AGC)  dosP ← oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,558,6850GT99.5% 665.2 / ‑4.3 209R436S (CGC→AGC) dosPoxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (105/102);  total (107/102)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

ATCATTATCGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCGATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTAT  >  CP009273/1558540‑1558828
                                                                                                                                                 |                                                                                                                                               
  cATTATCGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTg                                                                                                                                             >  2:3332574/1‑148 (MQ=255)
  cATTATCGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTg                                                                                                                                             >  2:1417758/1‑148 (MQ=255)
    ttATCGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATAATGATCCGGTTTGAGTTTTTCACGAAAGCTATTTAc                                                                                                                                           >  2:2615512/1‑148 (MQ=255)
      aTCGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGCCAAACTGCGTACCGTCGATACGCCAGAGCTACTGATCCGGGTTGAGTTTTTCACGACAGCGATTGAccc                                                                                                                                         >  1:1920535/1‑147 (MQ=255)
       tCGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCAc                                                                                                                                        >  2:30687/1‑148 (MQ=255)
        cGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGAGCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACt                                                                                                                                       >  2:3067633/1‑148 (MQ=255)
        cGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGAAACGACCGAGATACTGATCCGGTTTGAGTTTTTAACGAAAGCTATTGACCACt                                                                                                                                       >  1:1968612/1‑148 (MQ=255)
          gCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTc                                                                                                                                     <  1:2666360/148‑1 (MQ=255)
          gCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTc                                                                                                                                     <  1:2554953/148‑1 (MQ=255)
           cTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTcc                                                                                                                                    >  1:1630699/1‑148 (MQ=255)
           cTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTAACGAAAGCTATTGACCACTTcc                                                                                                                                    >  1:1626961/1‑148 (MQ=255)
           cTTGCTGACCACATTCCGTAGCTCATCGGCGAGTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTcc                                                                                                                                    >  2:1041638/1‑148 (MQ=255)
               cTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCa                                                                                                                                <  1:3111101/148‑1 (MQ=255)
                 gACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAAt                                                                                                                              >  2:3211205/1‑148 (MQ=255)
                  aCCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATg                                                                                                                             >  2:482160/1‑148 (MQ=255)
                      caTTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATAAGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTg                                                                                                                         >  1:1273725/1‑148 (MQ=255)
                         tCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATc                                                                                                                      <  2:1475676/148‑1 (MQ=255)
                             tAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGcc                                                                                                                  >  2:726286/1‑148 (MQ=255)
                               gCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCa                                                                                                                <  1:2922925/148‑1 (MQ=255)
                                cTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCAc                                                                                                               >  2:3340708/1‑148 (MQ=255)
                                 tCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACAGCGTAACATCGATACGACAGAGATACTGAACCGGTTTGAGTTTTTCACGAAATCTATTTACCACATCCAGCAAAGCCTTATCGGCCCACg                                                                                                              >  1:118621/1‑148 (MQ=255)
                                     cGGCGATTTGGGTAATGTTACTTACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAAAGCCTGATCGGCCCACGCATa                                                                                                          >  2:1183657/1‑148 (MQ=255)
                                     cGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATa                                                                                                          >  2:1183620/1‑148 (MQ=255)
                                     cGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATa                                                                                                          >  2:710051/1‑148 (MQ=255)
                                      ggCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAg                                                                                                         <  2:1731215/148‑1 (MQ=255)
                                        cGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGcc                                                                                                       <  2:1620486/148‑1 (MQ=255)
                                          aTTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCaa                                                                                                     <  1:426981/148‑1 (MQ=255)
                                          aTTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCaa                                                                                                     >  2:1318400/1‑148 (MQ=255)
                                           tttGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAg                                                                                                    <  1:740281/148‑1 (MQ=255)
                                              gggTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCt                                                                                                 >  1:1672990/1‑148 (MQ=255)
                                               ggTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTa                                                                                                <  2:1232642/148‑1 (MQ=255)
                                                  aaTGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTatca                                                                                             <  2:2956081/148‑1 (MQ=255)
                                                    tGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTatcaat                                                                                           >  2:1718781/1‑148 (MQ=255)
                                                    tGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTatcaat                                                                                           <  2:585599/148‑1 (MQ=255)
                                                    tGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTatcaat                                                                                           >  1:1512438/1‑148 (MQ=255)
                                                    tGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACACAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAAAGCCTGATCGGCCCACGCATAGCCAAGGCTatcaat                                                                                           >  1:2171179/1‑148 (MQ=255)
                                                     gTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTatcaatc                                                                                          >  1:3457318/1‑148 (MQ=255)
                                                     gTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTatcaatc                                                                                          <  1:2114137/148‑1 (MQ=255)
                                                      ttACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTatcaatca                                                                                         <  1:2547109/148‑1 (MQ=255)
                                                       tACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCGTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATcac                                                                                        <  2:2536031/148‑1 (MQ=255)
                                                          tGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATcacaac                                                                                     >  1:3253034/1‑146 (MQ=255)
                                                          tGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATc                                                                                     <  1:1417758/148‑1 (MQ=255)
                                                           gACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATcc                                                                                    >  2:1583742/1‑148 (MQ=255)
                                                           gACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATcc                                                                                    <  2:800847/148‑1 (MQ=255)
                                                             cgtcgtTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTg                                                                                  >  1:1462743/1‑148 (MQ=255)
                                                              gtcgtTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGa                                                                                 <  1:1351116/148‑1 (MQ=255)
                                                                cgtTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAat                                                                               >  2:17092/1‑148 (MQ=255)
                                                                 gtTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAata                                                                              <  2:2172775/148‑1 (MQ=255)
                                                                   tttCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATg                                                                            >  2:2722692/1‑148 (MQ=255)
                                                                   tttCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATg                                                                            >  1:3378595/1‑148 (MQ=255)
                                                                        aGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCaa                                                                       <  1:1123452/148‑1 (MQ=255)
                                                                         ggCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAac                                                                      >  2:1386946/1‑148 (MQ=255)
                                                                         ggCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAac                                                                      >  2:3133647/1‑148 (MQ=255)
                                                                           cTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAacac                                                                    <  2:2836419/148‑1 (MQ=255)
                                                                              aCGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGa                                                                 >  2:2645197/1‑148 (MQ=255)
                                                                                  ggACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATgag                                                             <  2:2303122/148‑1 (MQ=255)
                                                                                  ggACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATgag                                                             <  2:306952/148‑1 (MQ=255)
                                                                                       aaCTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATaca                                                        >  2:1154859/1‑148 (MQ=255)
                                                                                       aaCTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATaca                                                        >  1:1832273/1‑148 (MQ=255)
                                                                                           gCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATAcaccac                                                    <  1:197864/148‑1 (MQ=255)
                                                                                             gTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACgg                                                  <  2:68389/148‑1 (MQ=255)
                                                                                              tACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACggg                                                 >  1:3216420/1‑148 (MQ=255)
                                                                                               aCCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGga                                                >  2:936269/1‑148 (MQ=255)
                                                                                                ccTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGgag                                               >  2:1835917/1‑148 (MQ=255)
                                                                                                ccTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGgag                                               <  1:633177/148‑1 (MQ=255)
                                                                                                   tCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGgagaga                                            <  1:204845/148‑1 (MQ=255)
                                                                                                      aTACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACgg                                         >  1:1083657/1‑148 (MQ=255)
                                                                                                       tACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGc                                        <  2:2216485/148‑1 (MQ=255)
                                                                                                        aCGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCt                                       <  1:3297874/148‑1 (MQ=255)
                                                                                                        aCGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCt                                       >  1:246855/1‑148 (MQ=255)
                                                                                                           aCAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTg                                    <  1:438878/148‑1 (MQ=255)
                                                                                                             agagATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTc                                  <  2:3494004/148‑1 (MQ=255)
                                                                                                               agaTACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGa                                >  2:2318412/1‑148 (MQ=255)
                                                                                                                gaTACTGATCCGGTTTGAGTTTTTCACGAAATCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATTTTAAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGAc                               <  1:3340476/148‑1 (MQ=255)
                                                                                                                 aTACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGAcc                              <  2:194337/148‑1 (MQ=255)
                                                                                                                   aCTGATCCGGTTTGATTTTTTCACGAAAGCTATAGACCACTTACAGCAATGCATGATCGTCCCACGCATATCCAATGCTATCAATAACAACATGAATATGGTCAACACCTATGAGATACACCACGGGAGAGACGGCTTTGTCTACCAg                            >  2:2564848/1‑148 (MQ=255)
                                                                                                                   aCTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAg                            <  1:74804/148‑1 (MQ=255)
                                                                                                                    cTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGTCCAgg                           >  2:2254760/1‑148 (MQ=255)
                                                                                                                      gATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTc                         >  1:834386/1‑148 (MQ=255)
                                                                                                                      gATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTc                         >  1:2592302/1‑148 (MQ=255)
                                                                                                                        tCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCAt                       <  2:1968612/148‑1 (MQ=255)
                                                                                                                          cGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCg                     <  2:2157333/148‑1 (MQ=255)
                                                                                                                           ggTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGa                    <  1:976308/148‑1 (MQ=255)
                                                                                                                            gTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAg                   <  1:1036841/148‑1 (MQ=255)
                                                                                                                            gTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAg                   <  2:1077099/148‑1 (MQ=255)
                                                                                                                               tGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGtc                >  1:2281667/1‑147 (MQ=255)
                                                                                                                               tGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTa                <  2:1672990/148‑1 (MQ=255)
                                                                                                                                gAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTaa               <  2:3301126/148‑1 (MQ=255)
                                                                                                                                gAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATAAGGAATATGTTCAACACCGATGAGATACACCACGTGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTaa               >  1:173760/1‑148 (MQ=255)
                                                                                                                                  gTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAAtt             <  2:660962/148‑1 (MQ=255)
                                                                                                                                   tttttCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATtg            <  1:2416085/148‑1 (MQ=255)
                                                                                                                                   tttttCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATtg            >  2:894465/1‑148 (MQ=255)
                                                                                                                                    ttttCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATtgt           >  1:1478745/1‑148 (MQ=255)
                                                                                                                                         aCGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAgg      <  1:1522460/148‑1 (MQ=255)
                                                                                                                                          cGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt     >  1:257795/1‑148 (MQ=255)
                                                                                                                                          cGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt     >  1:1426657/1‑148 (MQ=255)
                                                                                                                                           gAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGtt    >  1:891021/1‑148 (MQ=255)
                                                                                                                                            aaaGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTa   <  2:43201/148‑1 (MQ=255)
                                                                                                                                            aaaGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTa   <  1:3067633/148‑1 (MQ=255)
                                                                                                                                             aaGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTAGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTAt  >  2:2460310/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ATCATTATCGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCGATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTAT  >  CP009273/1558540‑1558828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCAATCATTATCGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCGATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAT  >  CP009273/1558536‑1558834
                                                                                                                                                     |                                                                                                                                                     
GTCAATCATTATCGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCT                                                                                                                                                       <  SRR19973065.1137829/150‑1 (MQ=60)
  CAATCATTATCGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTAGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTAT                                                                                                                                                     >  SRR19973065.1044007/1‑150 (MQ=60)
   AATCATTATCGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATT                                                                                                                                                    <  SRR19973065.2565316/150‑1 (MQ=60)
   AATCATTATCGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATT                                                                                                                                                    >  SRR19973065.3515241/1‑150 (MQ=60)
    ATCATTATCGGCTTGCTGACCACAGTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTG                                                                                                                                                   <  SRR19973065.1977494/150‑1 (MQ=60)
        TTATCGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGCCAAACTGCGTACCGTCGATACGCCAGAGCTACTGATCCGGGTTGAGTTTTTCACGACAGCGATTGACCC                                                                                                                                               >  SRR19973065.1933132/1‑150 (MQ=60)
          ATCGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGAAACGACCGAGATACTGAGCCGGTTTGAGTTTTTAACGAAAGCTATTGACCACT                                                                                                                                             >  SRR19973065.1981452/1‑150 (MQ=60)
             GGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCC                                                                                                                                          >  SRR19973065.1641635/1‑150 (MQ=60)
             GGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCC                                                                                                                                          >  SRR19973065.1637810/1‑150 (MQ=60)
             GGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTAACGAAAGCTATTGACCACTTCC                                                                                                                                          >  SRR19973065.1637863/1‑150 (MQ=60)
              GCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCA                                                                                                                                         <  SRR19973065.2682865/150‑1 (MQ=60)
              GCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCA                                                                                                                                         <  SRR19973065.2570975/150‑1 (MQ=60)
              GCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCA                                                                                                                                         <  SRR19973065.2570937/150‑1 (MQ=60)
                   CTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAAT                                                                                                                                    <  SRR19973065.3130065/150‑1 (MQ=60)
                    TGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGCCAAACAGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGTCCACATCCAGCAAAG                                                                                                                                   >  SRR19973065.606252/1‑150 (MQ=60)
                    TGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATG                                                                                                                                   >  SRR19973065.820887/1‑150 (MQ=60)
                        CACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATAAGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTG                                                                                                                               >  SRR19973065.1282124/1‑150 (MQ=60)
                           ATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATC                                                                                                                            <  SRR19973065.1531388/150‑1 (MQ=60)
                                GTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCC                                                                                                                       <  SRR19973065.1728472/150‑1 (MQ=60)
                                  AGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCAC                                                                                                                     <  SRR19973065.228693/150‑1 (MQ=60)
                                   GCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACG                                                                                                                    >  SRR19973065.1429629/1‑150 (MQ=60)
                                   GCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACG                                                                                                                    <  SRR19973065.2940687/150‑1 (MQ=60)
                                   GCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACAGCGTAACATCGATACGACAGAGATACTGAACCGGTTTGAGTTTTTCACGAAATCTATTTACCACATCCAGCAAAGCCTTATCGGCCCACG                                                                                                                    >  SRR19973065.119666/1‑150 (MQ=60)
                                       ATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATA                                                                                                                <  SRR19973065.921519/150‑1 (MQ=60)
                                             GATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAG                                                                                                          <  SRR19973065.1486832/150‑1 (MQ=60)
                                             GATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAG                                                                                                          >  SRR19973065.2986843/1‑150 (MQ=60)
                                             GATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAG                                                                                                          <  SRR19973065.3226674/150‑1 (MQ=60)
                                              ATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGG                                                                                                         <  SRR19973065.429948/150‑1 (MQ=60)
                                               TTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGC                                                                                                        <  SRR19973065.745233/150‑1 (MQ=60)
                                                TTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCT                                                                                                       >  SRR19973065.1684178/1‑150 (MQ=60)
                                                  GGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTAT                                                                                                     >  SRR19973065.2669178/1‑150 (MQ=60)
                                                      AATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAAT                                                                                                 >  SRR19973065.3273699/1‑150 (MQ=60)
                                                      AATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAAT                                                                                                 >  SRR19973065.1522302/1‑150 (MQ=60)
                                                      AATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACACAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAAAGCCTGATCGGCCCACGCATAGCCAAGGCTATCAAT                                                                                                 >  SRR19973065.2185113/1‑150 (MQ=60)
                                                       ATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATC                                                                                                >  SRR19973065.3478385/1‑150 (MQ=60)
                                                        TGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCA                                                                                               >  SRR19973065.1451466/1‑150 (MQ=60)
                                                         GTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCAC                                                                                              <  SRR19973065.2127744/150‑1 (MQ=60)
                                                          TTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACA                                                                                             <  SRR19973065.2563103/150‑1 (MQ=60)
                                                          TTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACA                                                                                             <  SRR19973065.2563058/150‑1 (MQ=60)
                                                            ACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACAAC                                                                                           >  SRR19973065.3272700/1‑150 (MQ=60)
                                                             CTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCC                                                                                          <  SRR19973065.731152/150‑1 (MQ=60)
                                                              TGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCT                                                                                         <  SRR19973065.1427014/150‑1 (MQ=60)
                                                               GACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTG                                                                                        >  SRR19973065.1472141/1‑150 (MQ=60)
                                                                ACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGA                                                                                       <  SRR19973065.1100347/150‑1 (MQ=60)
                                                                  GTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAAT                                                                                     >  SRR19973065.1890727/1‑150 (MQ=60)
                                                                  GTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAAT                                                                                     >  SRR19973065.1657331/1‑150 (MQ=60)
                                                                  GTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAAT                                                                                     <  SRR19973065.1360055/150‑1 (MQ=60)
                                                                   TCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATA                                                                                    >  SRR19973065.3335049/1‑150 (MQ=60)
                                                                     GTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATG                                                                                  >  SRR19973065.3399248/1‑150 (MQ=60)
                                                                      TTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGG                                                                                 <  SRR19973065.1758138/150‑1 (MQ=60)
                                                                           GAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAAC                                                                            >  SRR19973065.3213811/1‑150 (MQ=60)
                                                                            AGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACA                                                                           <  SRR19973065.1130771/150‑1 (MQ=60)
                                                                            AGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACA                                                                           <  SRR19973065.2893238/150‑1 (MQ=60)
                                                                                  ACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATG                                                                     <  SRR19973065.757181/150‑1 (MQ=60)
                                                                                        ACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATAC                                                               >  SRR19973065.2163919/1‑150 (MQ=60)
                                                                                         CAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACA                                                              >  SRR19973065.1844348/1‑150 (MQ=60)
                                                                                         CAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACA                                                              >  SRR19973065.2442851/1‑150 (MQ=60)
                                                                                               GCGTACCTTCGATACGTCAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGG                                                        <  SRR19973065.200766/150‑1 (MQ=60)
                                                                                               GCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGG                                                        <  SRR19973065.199392/150‑1 (MQ=60)
                                                                                               GCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGG                                                        <  SRR19973065.199402/150‑1 (MQ=60)
                                                                                                CGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGG                                                       <  SRR19973065.1623752/150‑1 (MQ=60)
                                                                                                CGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGG                                                       >  SRR19973065.3235763/1‑150 (MQ=60)
                                                                                                AGTACCTTCGATACGACAGAGATACTGATCCTGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGG                                                       <  SRR19973065.1327120/150‑1 (MQ=60)
                                                                                                 GTACCTTCGATACGACATAGATACTGATCCGTTTTGAGTTTTTCACGAATGCTATTGACCACTTCAAGCAATGCCTGATCGGCCCACGCATCGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGA                                                      <  SRR19973065.316933/150‑1 (MQ=60)
                                                                                                 GTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGA                                                      <  SRR19973065.3087588/150‑1 (MQ=60)
                                                                                                   ACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGA                                                    <  SRR19973065.1369167/150‑1 (MQ=60)
                                                                                                    CCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAG                                                   <  SRR19973065.637302/150‑1 (MQ=60)
                                                                                                       TCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACG                                                <  SRR19973065.206408/150‑1 (MQ=60)
                                                                                                       TCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACG                                                <  SRR19973065.206860/150‑1 (MQ=60)
                                                                                                        CGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGG                                               >  SRR19973065.1090809/1‑150 (MQ=60)
                                                                                                          ATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCT                                             >  SRR19973065.248599/1‑150 (MQ=60)
                                                                                                            ACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTT                                           <  SRR19973065.3317957/150‑1 (MQ=60)
                                                                                                               ACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTC                                        <  SRR19973065.441751/150‑1 (MQ=60)
                                                                                                               ACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTC                                        <  SRR19973065.441919/150‑1 (MQ=60)
                                                                                                                CAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCG                                       >  SRR19973065.1239316/1‑150 (MQ=60)
                                                                                                                   AGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACC                                    <  SRR19973065.2232411/150‑1 (MQ=60)
                                                                                                                   AGATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACC                                    >  SRR19973065.1492107/1‑150 (MQ=60)
                                                                                                                    GATACTGATCCGGTTTGAGTTTTTCACGAAATCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATTTTAAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCA                                   <  SRR19973065.3360884/150‑1 (MQ=60)
                                                                                                                    GATACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCA                                   <  SRR19973065.3361119/150‑1 (MQ=60)
                                                                                                                       ACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGT                                <  SRR19973065.75570/150‑1 (MQ=60)
                                                                                                                       ACTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGT                                <  SRR19973065.74979/150‑1 (MQ=60)
                                                                                                                        CTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTC                               >  SRR19973065.839830/1‑150 (MQ=60)
                                                                                                                        CTGATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTC                               >  SRR19973065.2608427/1‑150 (MQ=60)
                                                                                                                          GATCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCAT                             >  SRR19973065.2351045/1‑150 (MQ=60)
                                                                                                                            TCCGGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCG                           >  SRR19973065.1638107/1‑150 (MQ=60)
                                                                                                                               GGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGG                        <  SRR19973065.982793/150‑1 (MQ=60)
                                                                                                                               GGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGG                        >  SRR19973065.3242460/1‑150 (MQ=60)
                                                                                                                               GGTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGG                        >  SRR19973065.2513713/1‑150 (MQ=60)
                                                                                                                                GTTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGT                       <  SRR19973065.1043708/150‑1 (MQ=60)
                                                                                                                                 TTTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTC                      >  SRR19973065.2296059/1‑150 (MQ=60)
                                                                                                                                  TTGAGTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATAAGGAATATGTTCAACACCGATGAGATACACCACGTGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAA                     >  SRR19973065.175146/1‑150 (MQ=60)
                                                                                                                                      GTTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGT                 >  SRR19973065.1488197/1‑150 (MQ=60)
                                                                                                                                       TTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTG                <  SRR19973065.2431306/150‑1 (MQ=60)
                                                                                                                                       TTTTTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTG                <  SRR19973065.1193110/150‑1 (MQ=60)
                                                                                                                                          TTCACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAG             >  SRR19973065.3450774/1‑150 (MQ=60)
                                                                                                                                            CACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGT           >  SRR19973065.259583/1‑150 (MQ=60)
                                                                                                                                            CACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGT           >  SRR19973065.260152/1‑150 (MQ=60)
                                                                                                                                            CACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGT           >  SRR19973065.1435949/1‑150 (MQ=60)
                                                                                                                                             ACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTT          >  SRR19973065.896671/1‑150 (MQ=60)
                                                                                                                                             ACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTT          >  SRR19973065.896376/1‑150 (MQ=60)
                                                                                                                                             ACGAAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTT          <  SRR19973065.1532438/150‑1 (MQ=60)
                                                                                                                                                AAAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATT       <  SRR19973065.3086388/150‑1 (MQ=60)
                                                                                                                                                 AAGCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTG      >  SRR19973065.457770/1‑150 (MQ=60)
                                                                                                                                                   GCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTCATTGTGCAGGTTATTGCG    >  SRR19973065.1814992/1‑150 (MQ=60)
                                                                                                                                                   GCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCG    <  SRR19973065.714796/150‑1 (MQ=60)
                                                                                                                                                   GCTATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCG    <  SRR19973065.2975632/150‑1 (MQ=60)
                                                                                                                                                   GCTATTGACCACGTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCG    <  SRR19973065.2208733/150‑1 (MQ=60)
                                                                                                                                                     TATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAT  <  SRR19973065.2128791/150‑1 (MQ=60)
                                                                                                                                                     TATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAT  >  SRR19973065.310005/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GTCAATCATTATCGGCTTGCTGACCACATTCCGTAGCTCATCGGCGATTTGGGTAATGTTACTGACGTCGTTTTCGAGGCTCACGAGGACAAACTGCGTACCTTCGATACGACAGAGATACTGATCCGGTTTGAGTTTTTCACGAAAGCGATTGACCACTTCCAGCAATGCCTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAT  >  CP009273/1558536‑1558834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑