Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,569,502 T→G 100% intergenic (‑43/+2) pqqL ← / ← yddB putative periplasmic M16 family zinc metalloendopeptidase/putative TonB‑dependent outer membrane receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,569,5020TG97.9% 737.1 / ‑1.1 186intergenic (‑43/+2)pqqL/yddBputative periplasmic M16 family zinc metalloendopeptidase/putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base G (97/85);  total (99/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 186 total aligned reads displayed.

ACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGC  >  CP009273/1569357‑1569642
                                                                                                                                                 |                                                                                                                                            
   tttCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTaa                                                                                                                                         >  2:1638199/1‑148 (MQ=255)
   tttCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTaa                                                                                                                                         >  2:1638250/1‑148 (MQ=255)
     tCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGTCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTaaaa                                                                                                                                       >  1:1875365/1‑148 (MQ=255)
      cATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACCTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGCTTAAGTAAAATCCGAACGATTAAAAt                                                                                                                                      <  2:2716986/148‑1 (MQ=255)
        tcgtGCTGTTAGGCGGCGGCAATGCGACGCCCGGGGAGCAAAAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATATTTATCTCCATGCGAAATCCGGGCGAATTTACCCGGTTTAGTAAAATCCGAAAGATTAAAAttt                                                                                                                                    <  2:1301247/145‑1 (MQ=255)
         ccTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTc                                                                                                                                   <  2:1328081/148‑1 (MQ=255)
         ccTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTc                                                                                                                                   >  2:3344253/1‑148 (MQ=255)
           tGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCAt                                                                                                                                 >  2:2763997/1‑148 (MQ=255)
           tGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGGTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCAt                                                                                                                                 <  2:1379499/148‑1 (MQ=255)
            gCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATg                                                                                                                                <  2:2098990/148‑1 (MQ=255)
               gTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTg                                                                                                                             <  1:446237/148‑1 (MQ=255)
                  aGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACa                                                                                                                          <  2:1990382/148‑1 (MQ=255)
                   ggcggcggcAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACAt                                                                                                                         >  2:1969936/1‑148 (MQ=255)
                    gcggcggcAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATc                                                                                                                        >  1:1513332/1‑148 (MQ=255)
                       gcggcAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAg                                                                                                                     <  2:77258/148‑1 (MQ=255)
                            aaTCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGa                                                                                                                <  1:1714258/148‑1 (MQ=255)
                              tCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACAGGGCGAATTTACCAGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGaaa                                                                                                              >  1:3480377/1‑148 (MQ=255)
                               cAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATCATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAg                                                                                                             <  2:1115391/148‑1 (MQ=255)
                                 gccgccCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTa                                                                                                           >  2:3250034/1‑148 (MQ=255)
                                   cgccCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGACAGTACg                                                                                                         >  2:3348677/1‑148 (MQ=255)
                                   cgccCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACg                                                                                                         <  1:900027/148‑1 (MQ=255)
                                     cccGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGc                                                                                                       >  1:1654222/1‑148 (MQ=255)
                                       cGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCa                                                                                                     >  1:3065073/1‑148 (MQ=255)
                                          ggAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTg                                                                                                  <  1:2785847/148‑1 (MQ=255)
                                              cAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGt                                                                                              <  2:3325889/148‑1 (MQ=255)
                                                 cAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAgg                                                                                           >  2:1565667/1‑148 (MQ=255)
                                                 cAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAgg                                                                                           >  2:3399170/1‑148 (MQ=255)
                                                     gTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGc                                                                                       <  1:2656757/148‑1 (MQ=255)
                                                     gTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGc                                                                                       <  1:916873/148‑1 (MQ=255)
                                                     gTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGc                                                                                       >  2:606735/1‑148 (MQ=255)
                                                      ttGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGcc                                                                                      >  2:3415581/1‑148 (MQ=255)
                                                          cACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCacac                                                                                  <  2:1143592/148‑1 (MQ=255)
                                                             tAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCgg                                                                               >  1:223172/1‑148 (MQ=255)
                                                             tAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCgg                                                                               >  1:355688/1‑148 (MQ=255)
                                                             tAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCgg                                                                               >  1:2124978/1‑148 (MQ=255)
                                                             tAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCgg                                                                               <  2:2636398/148‑1 (MQ=255)
                                                                cGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGgtgt                                                                            <  1:2847035/148‑1 (MQ=255)
                                                                cGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGgtgt                                                                            >  2:2849934/1‑148 (MQ=255)
                                                                   cAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCg                                                                         <  2:223172/148‑1 (MQ=255)
                                                                   cAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCg                                                                         >  2:2556675/1‑148 (MQ=255)
                                                                   cAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCg                                                                         >  2:1193980/1‑148 (MQ=255)
                                                                      tAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTg                                                                      >  2:63881/1‑148 (MQ=255)
                                                                       aaGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGt                                                                     >  2:3204037/1‑148 (MQ=255)
                                                                         gAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTAt                                                                   >  1:687081/1‑148 (MQ=255)
                                                                           aaCAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCa                                                                 <  2:517407/148‑1 (MQ=255)
                                                                           aaCAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCa                                                                 <  2:517410/148‑1 (MQ=255)
                                                                               gagGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAg                                                             >  1:1116476/1‑148 (MQ=255)
                                                                                  gTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCgg                                                          >  1:1061151/1‑148 (MQ=255)
                                                                                   tttCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGt                                                         <  1:23609/148‑1 (MQ=255)
                                                                                    ttCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGtt                                                        <  1:2763997/148‑1 (MQ=255)
                                                                                    ttCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGtt                                                        <  1:2763877/148‑1 (MQ=255)
                                                                                     tctcATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGttt                                                       <  1:3386518/148‑1 (MQ=255)
                                                                                     tctcATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGttt                                                       >  1:2239195/1‑148 (MQ=255)
                                                                                        cATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGc                                                    >  1:722664/1‑148 (MQ=255)
                                                                                          tAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTa                                                  >  1:644645/1‑148 (MQ=255)
                                                                                           aaTTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTAt                                                 >  1:2556326/1‑148 (MQ=255)
                                                                                            aTTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATc                                                >  2:453375/1‑148 (MQ=255)
                                                                                            aTTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATc                                                <  1:3198589/148‑1 (MQ=255)
                                                                                             ttATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAATCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGAGGTTTTGCTATcc                                               >  2:148598/1‑148 (MQ=255)
                                                                                               aTCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAg                                             >  2:3141283/1‑148 (MQ=255)
                                                                                               aTCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAg                                             >  2:3141498/1‑148 (MQ=255)
                                                                                               aTCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAg                                             >  2:739403/1‑148 (MQ=255)
                                                                                                 ctcCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTa                                           <  1:535913/148‑1 (MQ=255)
                                                                                                 ctcCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTa                                           <  1:3344258/148‑1 (MQ=255)
                                                                                                  tcCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTAc                                          >  2:2416559/1‑148 (MQ=255)
                                                                                                    cATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACAt                                        <  2:309775/148‑1 (MQ=255)
                                                                                                    cATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTCGCTATCCAGTACAt                                        <  1:1584563/148‑1 (MQ=255)
                                                                                                      tGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTg                                      <  2:3032937/148‑1 (MQ=255)
                                                                                                       gCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTga                                     <  1:1702038/148‑1 (MQ=255)
                                                                                                       gCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTga                                     >  2:219026/1‑148 (MQ=255)
                                                                                                       gCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTga                                     >  2:2301805/1‑148 (MQ=255)
                                                                                                        cGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTgag                                    <  2:2332170/148‑1 (MQ=255)
                                                                                                        cGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTgag                                    >  2:1778273/1‑148 (MQ=255)
                                                                                                         gAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTgaga                                   <  2:2286410/148‑1 (MQ=255)
                                                                                                         gAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATGgaga                                   <  2:2286408/148‑1 (MQ=255)
                                                                                                          aaaaCCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGaa                                  <  1:1789230/148‑1 (MQ=255)
                                                                                                           aaaCCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAat                                 <  1:1468555/148‑1 (MQ=255)
                                                                                                           aaaCCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAat                                 <  1:3262605/148‑1 (MQ=255)
                                                                                                            aaCCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAata                                <  2:616268/148‑1 (MQ=255)
                                                                                                             aCCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAatat                               <  2:319315/148‑1 (MQ=255)
                                                                                                             aCCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAatat                               >  2:1540350/1‑148 (MQ=255)
                                                                                                             aCCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAatat                               >  2:2677118/1‑148 (MQ=255)
                                                                                                             aCCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAatat                               <  1:1034268/148‑1 (MQ=255)
                                                                                                                  gCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCa                          <  2:1213286/148‑1 (MQ=255)
                                                                                                                    gAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACt                        <  2:2105153/148‑1 (MQ=255)
                                                                                                                      aTTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGa                      >  2:2821126/1‑148 (MQ=255)
                                                                                                                       tttACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACTGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGag                     >  2:2925758/1‑147 (MQ=255)
                                                                                                                       tttACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGAt                     <  2:37840/148‑1 (MQ=255)
                                                                                                                       tttACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGAt                     <  1:407057/148‑1 (MQ=255)
                                                                                                                          aCCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGt                  >  2:1855088/1‑148 (MQ=255)
                                                                                                                           cccGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTc                 >  2:244055/1‑148 (MQ=255)
                                                                                                                                ttAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGtt            >  2:1942868/1‑148 (MQ=255)
                                                                                                                                ttAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGtt            >  1:3413072/1‑148 (MQ=255)
                                                                                                                                ttAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGtt            <  2:835920/148‑1 (MQ=255)
                                                                                                                                   aGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTttg         <  1:1638242/148‑1 (MQ=255)
                                                                                                                                   aGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGGTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTttg         <  1:1638199/148‑1 (MQ=255)
                                                                                                                                    gTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTttgt        >  1:3440793/1‑148 (MQ=255)
                                                                                                                                     tAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTttgtt       >  1:1256263/1‑148 (MQ=255)
                                                                                                                                       aaaTCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTttgttgt     <  2:226824/148‑1 (MQ=255)
                                                                                                                                       aaaTCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTttgttgt     >  1:1914210/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
ACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGC  >  CP009273/1569357‑1569642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGT  >  CP009273/1569353‑1569651
                                                                                                                                                     |                                                                                                                                                     
ATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACG                                                                                                                                                       >  SRR19973065.1221130/1‑150 (MQ=60)
ATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACG                                                                                                                                                       >  SRR19973065.520825/1‑150 (MQ=60)
ATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACG                                                                                                                                                       >  SRR19973065.520828/1‑150 (MQ=60)
AATAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACG                                                                                                                                                       >  SRR19973065.321517/1‑150 (MQ=60)
  TAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGAT                                                                                                                                                     >  SRR19973065.2318139/1‑150 (MQ=60)
       TTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGTCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAA                                                                                                                                                >  SRR19973065.1887714/1‑150 (MQ=60)
        TTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAAT                                                                                                                                               >  SRR19973065.1915250/1‑150 (MQ=60)
         TCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATT                                                                                                                                              >  SRR19973065.36082/1‑150 (MQ=60)
                   GTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGAC                                                                                                                                    <  SRR19973065.449332/150‑1 (MQ=60)
                   GTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTAGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGAC                                                                                                                                    <  SRR19973065.1588272/150‑1 (MQ=60)
                      AGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATC                                                                                                                                 >  SRR19973065.1523212/1‑150 (MQ=60)
                                AATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAA                                                                                                                       <  SRR19973065.1725653/150‑1 (MQ=60)
                                AATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACAGGGCGAATTTACCAGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAA                                                                                                                       >  SRR19973065.3501558/1‑150 (MQ=60)
                                       CGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGC                                                                                                                >  SRR19973065.1665311/1‑150 (MQ=60)
                                       CGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGC                                                                                                                <  SRR19973065.905798/150‑1 (MQ=60)
                                         CCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCA                                                                                                              >  SRR19973065.3083813/1‑150 (MQ=60)
                                              GGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGC                                                                                                         <  SRR19973065.2801872/150‑1 (MQ=60)
                                              GGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGC                                                                                                         <  SRR19973065.2803126/150‑1 (MQ=60)
                                                     CAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTC                                                                                                  <  SRR19973065.734969/150‑1 (MQ=60)
                                                         GTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTACGTAAAATCCGAACGATTAAAAGTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCA                                                                                              <  SRR19973065.922861/150‑1 (MQ=60)
                                                         GTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCA                                                                                              <  SRR19973065.2673177/150‑1 (MQ=60)
                                                         GTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCA                                                                                              <  SRR19973065.922862/150‑1 (MQ=60)
                                                               ACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGG                                                                                        >  SRR19973065.358193/1‑150 (MQ=60)
                                                               ACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGG                                                                                        >  SRR19973065.2138656/1‑150 (MQ=60)
                                                               ACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGG                                                                                        >  SRR19973065.224827/1‑150 (MQ=60)
                                                               ACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGG                                                                                        >  SRR19973065.224873/1‑150 (MQ=60)
                                                               ACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGG                                                                                        >  SRR19973065.1606563/1‑150 (MQ=60)
                                                                 TAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTG                                                                                      <  SRR19973065.149431/150‑1 (MQ=60)
                                                                    CGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTC                                                                                   <  SRR19973065.2864556/150‑1 (MQ=60)
                                                                           AAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTAT                                                                            >  SRR19973065.691652/1‑150 (MQ=60)
                                                                           AAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTAT                                                                            >  SRR19973065.690846/1‑150 (MQ=60)
                                                                            AGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATC                                                                           >  SRR19973065.3485590/1‑150 (MQ=60)
                                                                            AGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATC                                                                           >  SRR19973065.1132811/1‑150 (MQ=60)
                                                                                 CAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAG                                                                      >  SRR19973065.1123766/1‑150 (MQ=60)
                                                                                    AGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGG                                                                   >  SRR19973065.1068186/1‑150 (MQ=60)
                                                                                       TTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTT                                                                <  SRR19973065.23927/150‑1 (MQ=60)
                                                                                       TTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTT                                                                >  SRR19973065.2253431/1‑150 (MQ=60)
                                                                                        TTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTT                                                               <  SRR19973065.2781053/150‑1 (MQ=60)
                                                                                        TTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTT                                                               <  SRR19973065.2781173/150‑1 (MQ=60)
                                                                                        TTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTT                                                               <  SRR19973065.2189252/150‑1 (MQ=60)
                                                                                         TCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTG                                                              <  SRR19973065.3407228/150‑1 (MQ=60)
                                                                                          CTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGC                                                             >  SRR19973065.2498738/1‑150 (MQ=60)
                                                                                          CTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGC                                                             >  SRR19973065.1681935/1‑150 (MQ=60)
                                                                                          CTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGC                                                             >  SRR19973065.727502/1‑150 (MQ=60)
                                                                                            CATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTA                                                           >  SRR19973065.648879/1‑150 (MQ=60)
                                                                                             ATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTAT                                                          >  SRR19973065.3519614/1‑150 (MQ=60)
                                                                                             ATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTAT                                                          >  SRR19973065.2572314/1‑150 (MQ=60)
                                                                                                ATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCA                                                       >  SRR19973065.715079/1‑150 (MQ=60)
                                                                                                ATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCA                                                       <  SRR19973065.3217857/150‑1 (MQ=60)
                                                                                                   ATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTA                                                    >  SRR19973065.3051327/1‑150 (MQ=60)
                                                                                                    TCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTAC                                                   >  SRR19973065.3460532/1‑150 (MQ=60)
                                                                                                     CTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACA                                                  <  SRR19973065.539406/150‑1 (MQ=60)
                                                                                                     CTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACA                                                  <  SRR19973065.3364693/150‑1 (MQ=60)
                                                                                                     CTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACA                                                  <  SRR19973065.3364688/150‑1 (MQ=60)
                                                                                                     CTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAACTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACA                                                  >  SRR19973065.323188/1‑150 (MQ=60)
                                                                                                        CATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTCGCTATCCAGTACATTG                                               <  SRR19973065.1595129/150‑1 (MQ=60)
                                                                                                         ATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGTCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGA                                              <  SRR19973065.1432056/150‑1 (MQ=60)
                                                                                                         ATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGTCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGA                                              <  SRR19973065.3187900/150‑1 (MQ=60)
                                                                                                           GCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGA                                            <  SRR19973065.1713385/150‑1 (MQ=60)
                                                                                                           GCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGA                                            <  SRR19973065.1713379/150‑1 (MQ=60)
                                                                                                              AAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATA                                         >  SRR19973065.2859895/1‑150 (MQ=60)
                                                                                                              AAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATA                                         <  SRR19973065.1800956/150‑1 (MQ=60)
                                                                                                              AAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATA                                         <  SRR19973065.1869497/150‑1 (MQ=60)
                                                                                                               AAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATAT                                        <  SRR19973065.1477974/150‑1 (MQ=60)
                                                                                                               AAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATAT                                        <  SRR19973065.3282376/150‑1 (MQ=60)
                                                                                                                 ACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCG                                      <  SRR19973065.1041114/150‑1 (MQ=60)
                                                                                                                   CGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGC                                    <  SRR19973065.2659945/150‑1 (MQ=60)
                                                                                                                   CGGGCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGC                                    <  SRR19973065.1072728/150‑1 (MQ=60)
                                                                                                                      GCGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACT                                 <  SRR19973065.1800406/150‑1 (MQ=60)
                                                                                                                       CGAATTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTG                                >  SRR19973065.2681893/1‑150 (MQ=60)
                                                                                                                           TTTACCCGGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCG                            <  SRR19973065.409909/150‑1 (MQ=60)
                                                                                                                                  GGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTT                     >  SRR19973065.430971/1‑150 (MQ=60)
                                                                                                                                  GGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTT                     >  SRR19973065.3433029/1‑150 (MQ=60)
                                                                                                                                  GGTTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTT                     >  SRR19973065.3433923/1‑150 (MQ=60)
                                                                                                                                    TTAAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTT                   >  SRR19973065.640359/1‑150 (MQ=60)
                                                                                                                                      AAGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGT                 >  SRR19973065.3461778/1‑150 (MQ=60)
                                                                                                                                       AGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTT                <  SRR19973065.1649244/150‑1 (MQ=60)
                                                                                                                                       AGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTT                >  SRR19973065.1264535/1‑150 (MQ=60)
                                                                                                                                       AGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTT                <  SRR19973065.1649252/150‑1 (MQ=60)
                                                                                                                                       AGTAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGGTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTT                <  SRR19973065.1649201/150‑1 (MQ=60)
                                                                                                                                         TAAAATCCGAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGT              >  SRR19973065.1926772/1‑150 (MQ=60)
                                                                                                                                                 GAACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAA      >  SRR19973065.2876535/1‑150 (MQ=60)
                                                                                                                                                   ACGATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATT    >  SRR19973065.3334108/1‑150 (MQ=60)
                                                                                                                                                     GATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGT  >  SRR19973065.1935632/1‑150 (MQ=60)
                                                                                                                                                     GATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGT  <  SRR19973065.472837/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGT  >  CP009273/1569353‑1569651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑