Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,816,601 A→T 100% H281L (CAC→CTC)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,816,6010AT100.0% 681.5 / NA 205H281L (CAC→CTC) spoTbifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (111/94);  total (111/94)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

TCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACC  >  CP009273/3816457‑3816744
                                                                                                                                                |                                                                                                                                               
tCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAg                                                                                                                                              >  1:1462063/1‑148 (MQ=255)
    tGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTg                                                                                                                                          >  2:2402468/1‑148 (MQ=255)
     ggTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGt                                                                                                                                         <  1:627552/148‑1 (MQ=255)
        cgcgAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACa                                                                                                                                      >  2:2701299/1‑148 (MQ=255)
          cgAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAg                                                                                                                                    <  1:2106730/148‑1 (MQ=255)
           gagaAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGc                                                                                                                                   <  1:539467/148‑1 (MQ=255)
              aaGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCcgc                                                                                                                                >  1:1685748/1‑148 (MQ=255)
               aGCATCTTTATTCCATTTACTGCAAAATGTTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATTCTCAGCCTGAACAAGCcgag                                                                                                                               >  2:1017522/1‑146 (MQ=255)
                gCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGt                                                                                                                              <  1:1797280/148‑1 (MQ=255)
                 cATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTc                                                                                                                             >  2:13052/1‑148 (MQ=255)
                 cATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTc                                                                                                                             <  1:3401856/148‑1 (MQ=255)
                   tCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCg                                                                                                                           <  2:3474856/148‑1 (MQ=255)
                    cTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCgg                                                                                                                          >  1:1237928/1‑148 (MQ=255)
                     tttATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCggg                                                                                                                         >  1:2177312/1‑148 (MQ=255)
                      ttATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGc                                                                                                                        <  1:2029869/148‑1 (MQ=255)
                           cGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGt                                                                                                                   >  2:2775320/1‑148 (MQ=255)
                            gATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTg                                                                                                                  <  2:2064692/148‑1 (MQ=255)
                              tttACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGaa                                                                                                                >  1:1585349/1‑148 (MQ=255)
                                tACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAg                                                                                                              >  2:848867/1‑148 (MQ=255)
                                   tGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACt                                                                                                           >  2:2892377/1‑148 (MQ=255)
                                    gCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACta                                                                                                          <  2:257937/148‑1 (MQ=255)
                                    gCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACta                                                                                                          <  2:111173/148‑1 (MQ=255)
                                    gCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACta                                                                                                          >  2:3489315/1‑148 (MQ=255)
                                     cAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACtat                                                                                                         >  1:648462/1‑148 (MQ=255)
                                     cAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACtat                                                                                                         >  1:2410224/1‑148 (MQ=255)
                                      aaaaTGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACtata                                                                                                        <  1:1828710/148‑1 (MQ=255)
                                        aaTGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATc                                                                                                      >  2:2619161/1‑148 (MQ=255)
                                        aaTGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATc                                                                                                      >  1:2887893/1‑148 (MQ=255)
                                         aTGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTCTATCg                                                                                                     >  2:3316051/1‑148 (MQ=255)
                                         aTGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCg                                                                                                     <  1:3009678/148‑1 (MQ=255)
                                             tGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAGGACTATATCGCCAt                                                                                                 <  1:3161912/148‑1 (MQ=255)
                                              gCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCAtt                                                                                                <  2:3051424/148‑1 (MQ=255)
                                               cTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTc                                                                                               <  1:1667125/148‑1 (MQ=255)
                                                tCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTcc                                                                                              >  1:429002/1‑148 (MQ=255)
                                                    agagCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCaaaa                                                                                          >  2:1961018/1‑148 (MQ=255)
                                                     gagCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAg                                                                                         >  1:2631095/1‑148 (MQ=255)
                                                        cagcGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGa                                                                                      <  1:68288/148‑1 (MQ=255)
                                                        cagcGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATAGCCATTCCAAAAGCGa                                                                                      >  1:1873857/1‑148 (MQ=255)
                                                         agcGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGaa                                                                                     >  1:2644418/1‑148 (MQ=255)
                                                         agcGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGaa                                                                                     >  1:1365936/1‑148 (MQ=255)
                                                           cGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACg                                                                                   >  2:2291760/1‑148 (MQ=255)
                                                              tttCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCt                                                                                >  2:1183551/1‑148 (MQ=255)
                                                              tttCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCt                                                                                >  2:1476821/1‑148 (MQ=255)
                                                                 cACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATc                                                                             <  2:3192773/148‑1 (MQ=255)
                                                                 cACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATc                                                                             >  2:2328862/1‑148 (MQ=255)
                                                                 cACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATc                                                                             >  2:1746370/1‑148 (MQ=255)
                                                                  aCTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCa                                                                            <  2:1583657/148‑1 (MQ=255)
                                                                  aCTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCa                                                                            <  2:1583168/148‑1 (MQ=255)
                                                                   cTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAg                                                                           <  2:1873679/148‑1 (MQ=255)
                                                                    tCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGt                                                                          >  2:316999/1‑148 (MQ=255)
                                                                     cGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTc                                                                         <  1:35149/148‑1 (MQ=255)
                                                                     cGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTc                                                                         >  2:2261944/1‑148 (MQ=255)
                                                                     cGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTc                                                                         <  1:1105263/148‑1 (MQ=255)
                                                                     cGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTc                                                                         >  2:498001/1‑148 (MQ=255)
                                                                      gATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCt                                                                        <  1:2494507/148‑1 (MQ=255)
                                                                        tCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCttt                                                                      <  1:3083724/148‑1 (MQ=255)
                                                                        tCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCttt                                                                      >  2:1080464/1‑148 (MQ=255)
                                                                        tCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCttt                                                                      >  1:283466/1‑148 (MQ=255)
                                                                        tCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCttt                                                                      >  2:2925016/1‑148 (MQ=255)
                                                                        tCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCttt                                                                      >  1:1541019/1‑148 (MQ=255)
                                                                          aTGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGc                                                                    >  2:2534815/1‑148 (MQ=255)
                                                                          aTGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGc                                                                    <  2:1640507/148‑1 (MQ=255)
                                                                            ggACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGcac                                                                  >  1:3424686/1‑148 (MQ=255)
                                                                                 tCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTc                                                             >  1:2625101/1‑148 (MQ=255)
                                                                                  cTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCg                                                            >  2:884728/1‑148 (MQ=255)
                                                                                  cTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCg                                                            <  2:1554566/148‑1 (MQ=255)
                                                                                  cTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCg                                                            <  2:1554765/148‑1 (MQ=255)
                                                                                  cTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCg                                                            <  1:2765764/148‑1 (MQ=255)
                                                                                      gCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCCGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgatga                                                        <  1:1903712/148‑1 (MQ=255)
                                                                                       cTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgatgat                                                       >  2:3437859/1‑148 (MQ=255)
                                                                                       cTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgatgat                                                       <  2:2035415/148‑1 (MQ=255)
                                                                                        tttCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATc                                                      <  2:1289994/148‑1 (MQ=255)
                                                                                           ccGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGc                                                   >  2:1133181/1‑148 (MQ=255)
                                                                                           ccGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGc                                                   <  1:1943257/148‑1 (MQ=255)
                                                                                           ccGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGc                                                   <  1:773804/148‑1 (MQ=255)
                                                                                                  aTCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACg                                            >  1:1015730/1‑148 (MQ=255)
                                                                                                      tCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGgtgt                                        >  1:1263771/1‑148 (MQ=255)
                                                                                                       cAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGtgtg                                       >  2:2170430/1‑148 (MQ=255)
                                                                                                        aaTGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGc                                      <  1:1961018/148‑1 (MQ=255)
                                                                                                           gATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCgg                                   <  1:1655523/148‑1 (MQ=255)
                                                                                                           gATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCgg                                   >  1:2468638/1‑148 (MQ=255)
                                                                                                                  acacCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGt                            >  1:505179/1‑148 (MQ=255)
                                                                                                                    acCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTcc                          <  1:2701299/148‑1 (MQ=255)
                                                                                                                      cTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAg                        <  2:1225440/148‑1 (MQ=255)
                                                                                                                         ttATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATc                     >  2:2212797/1‑148 (MQ=255)
                                                                                                                          tATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATcc                    >  1:1556340/1‑148 (MQ=255)
                                                                                                                          tATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATcc                    >  2:3402057/1‑148 (MQ=255)
                                                                                                                          tATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATcc                    >  2:663110/1‑148 (MQ=255)
                                                                                                                          tATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATcc                    >  2:175712/1‑148 (MQ=255)
                                                                                                                          tATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATcc                    >  1:3199440/1‑148 (MQ=255)
                                                                                                                          tATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATcc                    >  1:847197/1‑148 (MQ=255)
                                                                                                                          tATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGGTGAGGTCCAGATcc                    >  2:2421368/1‑148 (MQ=255)
                                                                                                                           atggcgTGGTGGGACAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCCGATCCg                   <  1:1751876/145‑1 (MQ=255)
                                                                                                                                   cTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGTCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAg           >  1:1426476/1‑148 (MQ=255)
                                                                                                                                     gggCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGat         <  1:912966/148‑1 (MQ=255)
                                                                                                                                     gggCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCAGTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGACGat         >  1:2433690/1‑148 (MQ=255)
                                                                                                                                       gCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGatat       <  2:2177312/148‑1 (MQ=255)
                                                                                                                                        ccAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATg      <  2:3500858/148‑1 (MQ=255)
                                                                                                                                          aGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGa    >  1:2249052/1‑148 (MQ=255)
                                                                                                                                            aTGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCGGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGAcc  >  1:2603762/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACC  >  CP009273/3816457‑3816744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGG  >  CP009273/3816455‑3816750
                                                                                                                                                  |                                                                                                                                                     
CGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAG                                                                                                                                                    >  SRR19973065.1471458/1‑150 (MQ=60)
   CAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCT                                                                                                                                                 >  SRR19973065.1311837/1‑150 (MQ=60)
       GGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTAC                                                                                                                                             <  SRR19973065.631640/150‑1 (MQ=60)
        GTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACA                                                                                                                                            <  SRR19973065.1570052/150‑1 (MQ=60)
            CGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCC                                                                                                                                        <  SRR19973065.2120275/150‑1 (MQ=60)
             GAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCG                                                                                                                                       <  SRR19973065.542971/150‑1 (MQ=60)
              AGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGC                                                                                                                                      >  SRR19973065.1697014/1‑150 (MQ=60)
                AAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGT                                                                                                                                    <  SRR19973065.1694965/150‑1 (MQ=60)
                  GCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCC                                                                                                                                  <  SRR19973065.1809088/150‑1 (MQ=60)
                   CATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCG                                                                                                                                 <  SRR19973065.3422649/150‑1 (MQ=60)
                    ATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGG                                                                                                                                >  SRR19973065.1246053/1‑150 (MQ=60)
                    ATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGG                                                                                                                                >  SRR19973065.190047/1‑150 (MQ=60)
                     TCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGG                                                                                                                               >  SRR19973065.2191229/1‑150 (MQ=60)
                     TCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGG                                                                                                                               >  SRR19973065.2191275/1‑150 (MQ=60)
                        TTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCG                                                                                                                            <  SRR19973065.2042900/150‑1 (MQ=60)
                           TTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGT                                                                                                                         >  SRR19973065.2690933/1‑150 (MQ=60)
                              GATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAA                                                                                                                      >  SRR19973065.1595922/1‑150 (MQ=60)
                                  TACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGAC                                                                                                                  <  SRR19973065.63768/150‑1 (MQ=60)
                                   ACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACT                                                                                                                 <  SRR19973065.1565066/150‑1 (MQ=60)
                                   ACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACT                                                                                                                 <  SRR19973065.919/150‑1 (MQ=60)
                                   ACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACT                                                                                                                 <  SRR19973065.2640634/150‑1 (MQ=60)
                                     TGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTAT                                                                                                               >  SRR19973065.1451580/1‑150 (MQ=60)
                                     TGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTAT                                                                                                               >  SRR19973065.652726/1‑150 (MQ=60)
                                     TGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTAT                                                                                                               >  SRR19973065.2425399/1‑150 (MQ=60)
                                        AAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATC                                                                                                            >  SRR19973065.2905549/1‑150 (MQ=60)
                                        AAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATC                                                                                                            >  SRR19973065.2873755/1‑150 (MQ=60)
                                        AAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATC                                                                                                            <  SRR19973065.1840757/150‑1 (MQ=60)
                                          AATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCATTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGC                                                                                                          >  SRR19973065.1038548/1‑150 (MQ=60)
                                           ATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCC                                                                                                         <  SRR19973065.3028108/150‑1 (MQ=60)
                                              GTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATT                                                                                                      <  SRR19973065.2516149/150‑1 (MQ=60)
                                               TGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAGGACTATATCGCCATTC                                                                                                     <  SRR19973065.3181065/150‑1 (MQ=60)
                                                GCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCCGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCC                                                                                                    <  SRR19973065.2733099/150‑1 (MQ=60)
                                                GCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCC                                                                                                    >  SRR19973065.431980/1‑150 (MQ=60)
                                                 CTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCA                                                                                                   <  SRR19973065.3427788/150‑1 (MQ=60)
                                                 CTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCA                                                                                                   <  SRR19973065.1678281/150‑1 (MQ=60)
                                                   CAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAA                                                                                                 >  SRR19973065.2446665/1‑150 (MQ=60)
                                                     AAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAG                                                                                               <  SRR19973065.1664614/150‑1 (MQ=60)
                                                     AAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAG                                                                                               >  SRR19973065.2647360/1‑150 (MQ=60)
                                                     AAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAG                                                                                               >  SRR19973065.2647430/1‑150 (MQ=60)
                                                     AAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAG                                                                                               >  SRR19973065.3190898/1‑150 (MQ=60)
                                                     AAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAG                                                                                               >  SRR19973065.1941108/1‑150 (MQ=60)
                                                      AGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGC                                                                                              <  SRR19973065.376626/150‑1 (MQ=60)
                                                        AGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGA                                                                                            >  SRR19973065.1885775/1‑150 (MQ=60)
                                                        AGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATAGCCATTCCAAAAGCGA                                                                                            >  SRR19973065.1886195/1‑150 (MQ=60)
                                                         GCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAA                                                                                           >  SRR19973065.2660729/1‑150 (MQ=60)
                                                         GCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAA                                                                                           >  SRR19973065.1374967/1‑150 (MQ=60)
                                                          CAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAAC                                                                                          <  SRR19973065.69009/150‑1 (MQ=60)
                                                              GTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCT                                                                                      >  SRR19973065.3365602/1‑150 (MQ=60)
                                                                 TTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCCGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATC                                                                                   <  SRR19973065.1384930/150‑1 (MQ=60)
                                                                       CGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTT                                                                             <  SRR19973065.3070546/150‑1 (MQ=60)
                                                                       CGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTT                                                                             <  SRR19973065.82720/150‑1 (MQ=60)
                                                                       CGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTT                                                                             <  SRR19973065.35584/150‑1 (MQ=60)
                                                                       CGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTT                                                                             <  SRR19973065.1112508/150‑1 (MQ=60)
                                                                        GATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTT                                                                            >  SRR19973065.1551204/1‑150 (MQ=60)
                                                                        GATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTT                                                                            <  SRR19973065.211697/150‑1 (MQ=60)
                                                                        GATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTT                                                                            >  SRR19973065.285336/1‑150 (MQ=60)
                                                                        GATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTT                                                                            <  SRR19973065.2496481/150‑1 (MQ=60)
                                                                        GATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTT                                                                            <  SRR19973065.2510203/150‑1 (MQ=60)
                                                                          TCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGC                                                                          <  SRR19973065.3102556/150‑1 (MQ=60)
                                                                          TCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGC                                                                          <  SRR19973065.3103671/150‑1 (MQ=60)
                                                                            ATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCAC                                                                        >  SRR19973065.3445589/1‑150 (MQ=60)
                                                                                 CATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTANAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTC                                                                   >  SRR19973065.1794173/1‑150 (MQ=60)
                                                                                 CATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTC                                                                   >  SRR19973065.2641439/1‑150 (MQ=60)
                                                                                 CATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTC                                                                   >  SRR19973065.2641342/1‑150 (MQ=60)
                                                                                    CTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGAT                                                                <  SRR19973065.2782955/150‑1 (MQ=60)
                                                                                     TACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGATATGCCTGTACAAGCCGAGACCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGACAGGCTATCAGACTTTGCTCACCTCGTTG                                                               >  SRR19973065.995813/1‑150 (MQ=60)
                                                                                        GCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCCGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATC                                                            <  SRR19973065.1916222/150‑1 (MQ=60)
                                                                                        GCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCCGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATC                                                            <  SRR19973065.2222116/150‑1 (MQ=60)
                                                                                             CCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCC                                                       <  SRR19973065.1955959/150‑1 (MQ=60)
                                                                                             CCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCC                                                       <  SRR19973065.778946/150‑1 (MQ=60)
                                                                                             CCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCCCGATGATCGGCCC                                                       <  SRR19973065.778945/150‑1 (MQ=60)
                                                                                                  TGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACG                                                  >  SRR19973065.1022458/1‑150 (MQ=60)
                                                                                                  TGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACG                                                  >  SRR19973065.659919/1‑150 (MQ=60)
                                                                                                      CGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGT                                              >  SRR19973065.1272099/1‑150 (MQ=60)
                                                                                                      CGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGT                                              >  SRR19973065.1271769/1‑150 (MQ=60)
                                                                                                          AATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCG                                          <  SRR19973065.1973823/150‑1 (MQ=60)
                                                                                                          AATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCG                                          >  SRR19973065.3309110/1‑150 (MQ=60)
                                                                                                           ATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGG                                         >  SRR19973065.2484188/1‑150 (MQ=60)
                                                                                                             GATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTT                                       <  SRR19973065.2959904/150‑1 (MQ=60)
                                                                                                             GATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTT                                       <  SRR19973065.1666620/150‑1 (MQ=60)
                                                                                                             GATTCTGACACCTGTTATCGCGTGCTGGGCCAGAGGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTT                                       <  SRR19973065.1666652/150‑1 (MQ=60)
                                                                                                              ATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTG                                      <  SRR19973065.2278665/150‑1 (MQ=60)
                                                                                                               TTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGA                                     <  SRR19973065.578956/150‑1 (MQ=60)
                                                                                                                TCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAG                                    <  SRR19973065.3476846/150‑1 (MQ=60)
                                                                                                                TCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAG                                    <  SRR19973065.195993/150‑1 (MQ=60)
                                                                                                                  TGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGT                                  >  SRR19973065.508723/1‑150 (MQ=60)
                                                                                                                  TGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGT                                  >  SRR19973065.508550/1‑150 (MQ=60)
                                                                                                                   GACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTC                                 >  SRR19973065.2011260/1‑150 (MQ=60)
                                                                                                                    ACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCC                                >  SRR19973065.1811338/1‑150 (MQ=60)
                                                                                                                      ACCTGTTATCGCGTGCTGGGCCAGATGCTCCGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAG                              <  SRR19973065.2718083/150‑1 (MQ=60)
                                                                                                                       CCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGA                             >  SRR19973065.1489869/1‑150 (MQ=60)
                                                                                                                          GTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCC                          >  SRR19973065.1566664/1‑150 (MQ=60)
                                                                                                                          GTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCC                          >  SRR19973065.3218708/1‑150 (MQ=60)
                                                                                                                          GTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCC                          >  SRR19973065.852688/1‑150 (MQ=60)
                                                                                                                           TTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCG                         <  SRR19973065.2792549/150‑1 (MQ=60)
                                                                                                                             ATGGCGTGGTGGGACAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCCGATCCGTA                       <  SRR19973065.1763408/150‑1 (MQ=60)
                                                                                                                             ATCGCGTGCTGGGCCAGATGCTCCGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTA                       <  SRR19973065.44104/150‑1 (MQ=60)
                                                                                                                             ATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTA                       <  SRR19973065.1763374/150‑1 (MQ=60)
                                                                                                                                   TGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGTCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAG                 >  SRR19973065.1435768/1‑150 (MQ=60)
                                                                                                                                     CTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGAT               >  SRR19973065.2449077/1‑150 (MQ=60)
                                                                                                                                     CTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGAT               <  SRR19973065.1243713/150‑1 (MQ=60)
                                                                                                                                     CTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCAGTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGACGAT               >  SRR19973065.2449037/1‑150 (MQ=60)
                                                                                                                                       GGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATAT             <  SRR19973065.918914/150‑1 (MQ=60)
                                                                                                                                          CCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGA          >  SRR19973065.2263313/1‑150 (MQ=60)
                                                                                                                                            AGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCGGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACC        >  SRR19973065.2619928/1‑150 (MQ=60)
                                                                                                                                            AGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACC        <  SRR19973065.2042092/150‑1 (MQ=60)
                                                                                                                                                 CTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATG   >  SRR19973065.450500/1‑150 (MQ=60)
                                                                                                                                                  TCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATCTGGACCAGATGG  >  SRR19973065.461356/1‑150 (MQ=60)
                                                                                                                                                  TCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGG  <  SRR19973065.1632015/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
CGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGG  >  CP009273/3816455‑3816750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑