Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,954,364 G→T 100% D110Y (GAT→TAT)  rep → DNA helicase and single‑stranded DNA‑dependent ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,954,3640GT97.2% 649.3 / 8.4 211D110Y (GAT→TAT) repDNA helicase and single‑stranded DNA‑dependent ATPase
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base T (95/110);  total (98/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 214 total aligned reads displayed.

CGAGATGAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACGATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGATTG  >  CP009273/3954219‑3954508
                                                                                                                                                 |                                                                                                                                                
cgAGTTTAAAGAGCGTTTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCAATTCGTTGGGGCTGGATATCATAAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTAt                                                                                                                                                <  2:3438988/148‑1 (MQ=255)
cgAGATGAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTAt                                                                                                                                                <  2:2845207/148‑1 (MQ=255)
cgAGATGAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTAt                                                                                                                                                <  1:2701664/148‑1 (MQ=255)
 gagaTGAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACtata                                                                                                                                               <  2:785273/148‑1 (MQ=255)
     tGAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGa                                                                                                                                           <  1:2967845/148‑1 (MQ=255)
      gAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGAt                                                                                                                                          <  2:2432632/148‑1 (MQ=255)
      gAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGAt                                                                                                                                          <  2:1060512/148‑1 (MQ=255)
       aaaGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATc                                                                                                                                         <  2:3082896/148‑1 (MQ=255)
       aaaGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATc                                                                                                                                         <  2:1429349/148‑1 (MQ=255)
       aaaGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACGATACCGATc                                                                                                                                         <  2:3256998/148‑1 (MQ=255)
         agagCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAACGCGAACTTCTCGTTGTTTGACTATACCGATCAg                                                                                                                                       >  2:2069364/1‑148 (MQ=255)
         agagCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAg                                                                                                                                       >  2:1494374/1‑148 (MQ=255)
         agagCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAg                                                                                                                                       >  1:3116403/1‑148 (MQ=255)
          gagCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAgc                                                                                                                                      <  2:40661/148‑1 (MQ=255)
          gagCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAgc                                                                                                                                      >  1:1881544/1‑148 (MQ=255)
           agCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAgct                                                                                                                                     <  1:1020583/148‑1 (MQ=255)
             cGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAgcttg                                                                                                                                   >  1:798490/1‑148 (MQ=255)
                    ggCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCttgctttgct                                                                                                                            >  1:930239/1‑148 (MQ=255)
                       aGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTaa                                                                                                                         <  2:2565026/148‑1 (MQ=255)
                        gACGCTGGGGCACAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTaaa                                                                                                                        >  2:757261/1‑148 (MQ=255)
                         aCGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAag                                                                                                                       <  1:3351770/148‑1 (MQ=255)
                         aCGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAag                                                                                                                       <  1:3351978/148‑1 (MQ=255)
                         aCGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAag                                                                                                                       >  1:1803602/1‑148 (MQ=255)
                           gCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTGTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAagag                                                                                                                     <  2:1863560/148‑1 (MQ=255)
                             tGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGtt                                                                                                                   <  2:3148653/148‑1 (MQ=255)
                               gggCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGa                                                                                                                 <  1:1370898/148‑1 (MQ=255)
                               gggCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGa                                                                                                                 >  2:1308666/1‑148 (MQ=255)
                               gggCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGa                                                                                                                 <  1:1647399/148‑1 (MQ=255)
                                 gcgcAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGAcc                                                                                                               >  1:909105/1‑148 (MQ=255)
                                 gcgcAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGAcc                                                                                                               >  1:2419398/1‑148 (MQ=255)
                                  cgcAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCg                                                                                                              <  2:2887853/148‑1 (MQ=255)
                                       agagGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAgggg                                                                                                         >  2:651869/1‑148 (MQ=255)
                                       agagGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAgggg                                                                                                         >  1:969738/1‑148 (MQ=255)
                                       agagGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAgggg                                                                                                         <  2:690207/148‑1 (MQ=255)
                                       agagGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAgggg                                                                                                         <  1:315078/148‑1 (MQ=255)
                                         agGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCt                                                                                                       >  1:1528684/1‑148 (MQ=255)
                                          ggCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTg                                                                                                      <  1:3079903/148‑1 (MQ=255)
                                            cgcgTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGAt                                                                                                    <  2:2954426/148‑1 (MQ=255)
                                                tGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGaa                                                                                                >  2:3353369/1‑148 (MQ=255)
                                                tGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGaa                                                                                                <  2:1570051/148‑1 (MQ=255)
                                                tGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGaa                                                                                                >  2:1613111/1‑148 (MQ=255)
                                                tGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGaa                                                                                                <  1:1273755/148‑1 (MQ=255)
                                                  ggCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGa                                                                                              <  2:101311/148‑1 (MQ=255)
                                                       atgatCTCCACTGTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACa                                                                                         <  2:2102839/148‑1 (MQ=255)
                                                        tgatCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACaa                                                                                        >  1:1120747/1‑148 (MQ=255)
                                                        tgatCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACaa                                                                                        >  2:2491231/1‑148 (MQ=255)
                                                        tgatCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACaa                                                                                        >  1:2457962/1‑148 (MQ=255)
                                                          atCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAg                                                                                      <  2:368004/148‑1 (MQ=255)
                                                            ctcCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCNCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGtt                                                                                    >  1:1783264/1‑148 (MQ=255)
                                                              ccACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTtct                                                                                  >  2:1932617/1‑148 (MQ=255)
                                                              ccACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTtct                                                                                  >  2:2677471/1‑148 (MQ=255)
                                                                 cTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCt                                                                               >  1:131698/1‑148 (MQ=255)
                                                                     ccATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGcaa                                                                           >  2:3080909/1‑148 (MQ=255)
                                                                        tACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGcaacaa                                                                        <  1:1699600/148‑1 (MQ=255)
                                                                            ttGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGa                                                                    <  2:2305946/148‑1 (MQ=255)
                                                                            ttGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGa                                                                    <  1:2778828/148‑1 (MQ=255)
                                                                            ttGGGGCTGGATAGCATCAAACGCGAGTATGCGGCGCGGGGGATGAAAGCGAACTTCTCGTTGTTTGACTAGACCGCTCAGCTTGCTTTGCTGAAAGAGTTGACCGAGGGGCTGATGGACGATGACAAAGTTCTCCTGCACCACCTGa                                                                    >  2:486883/1‑148 (MQ=255)
                                                                                  cTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGa                                                              <  2:1867535/148‑1 (MQ=255)
                                                                                  cTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGa                                                              <  2:1870129/148‑1 (MQ=255)
                                                                                  cTGGATATCATCAAAAGCGAGTATGCGTCGCTTGGGATGATAGCGAAATTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGATTTGACCTAGTGGCTGATTGAAGATGACATAGTTCTCATGCAACAACTGATTTCGa                                                              <  2:79602/148‑1 (MQ=255)
                                                                                    ggATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGAcc                                                            <  1:724310/148‑1 (MQ=255)
                                                                                    ggATATCATCAAACGCGAGTATGCGGCGCTGGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGAcc                                                            >  2:2831298/1‑148 (MQ=255)
                                                                                       tatCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCAtc                                                         <  2:1692943/148‑1 (MQ=255)
                                                                                        atcatcAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCAtct                                                        >  1:421944/1‑148 (MQ=255)
                                                                                        atcatcAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCAtct                                                        >  1:2191990/1‑148 (MQ=255)
                                                                                         tcatcaAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCGTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCAtctc                                                       <  2:2664580/148‑1 (MQ=255)
                                                                                          catcaAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATctct                                                      >  1:2319795/1‑148 (MQ=255)
                                                                                          catcaAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATctct                                                      >  1:367591/1‑148 (MQ=255)
                                                                                           atcaAACGCGAGTATGCGGCGCTTGGGATGAAAGCTAACTTCTCTTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGTCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTa                                                     <  2:3042623/148‑1 (MQ=255)
                                                                                           atcaAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTa                                                     <  1:3122720/148‑1 (MQ=255)
                                                                                           atcaAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTa                                                     <  2:82345/148‑1 (MQ=255)
                                                                                           atcaAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAAGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTa                                                     <  1:1686820/148‑1 (MQ=255)
                                                                                             caAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAAc                                                   >  2:3006162/1‑148 (MQ=255)
                                                                                             caAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACGTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAAc                                                   <  2:2767756/148‑1 (MQ=255)
                                                                                              aaaCGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACt                                                  >  2:3057405/1‑148 (MQ=255)
                                                                                               aaCGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTg                                                 >  1:3210268/1‑148 (MQ=255)
                                                                                                 cgcgAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGga                                               <  1:1742123/148‑1 (MQ=255)
                                                                                                 cgcgAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGga                                               <  1:2436436/148‑1 (MQ=255)
                                                                                                 cgcgAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGga                                               <  1:558631/148‑1 (MQ=255)
                                                                                                   cgAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAATATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGgaag                                             >  1:2371004/1‑148 (MQ=255)
                                                                                                       tATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATg                                         >  1:2969691/1‑148 (MQ=255)
                                                                                                        aTGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGa                                        <  1:3247431/148‑1 (MQ=255)
                                                                                                         tGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGAt                                       <  2:2266246/148‑1 (MQ=255)
                                                                                                          gcggcgCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGAtc                                      >  1:1420060/1‑148 (MQ=255)
                                                                                                           cggcgCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGAtct                                     <  1:2973121/148‑1 (MQ=255)
                                                                                                             gcgcTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCa                                   >  1:3487811/1‑148 (MQ=255)
                                                                                                                cTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCaaaa                                >  2:3200668/1‑148 (MQ=255)
                                                                                                                cTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCaaaa                                <  1:2000504/148‑1 (MQ=255)
                                                                                                                    ggATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACAcc                            <  2:3391503/148‑1 (MQ=255)
                                                                                                                     gATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCg                           >  1:491336/1‑148 (MQ=255)
                                                                                                                     gATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCg                           <  1:625261/148‑1 (MQ=255)
                                                                                                                       tGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTc                         <  2:2166326/148‑1 (MQ=255)
                                                                                                                         aaaGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTccc                       >  2:2773914/1‑148 (MQ=255)
                                                                                                                           ggCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAg                     >  1:2980433/2‑148 (MQ=255)
                                                                                                                                 cTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGgca               >  2:17601/1‑148 (MQ=255)
                                                                                                                                 cTTCTCGTTGTTTGACGATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAAAACCGTCCCAGGCGgca               >  2:2818967/1‑148 (MQ=255)
                                                                                                                                      cGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTGAAAACACCGTCCCAGGCGGCAGCAAg          >  1:1089247/1‑148 (MQ=255)
                                                                                                                                         tgttTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGc       >  1:2521707/1‑148 (MQ=255)
                                                                                                                                            ttGACTATACAGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGAt    <  1:3230776/148‑1 (MQ=255)
                                                                                                                                              gACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGATTg  <  2:968729/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGAGATGAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACGATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGATTG  >  CP009273/3954219‑3954508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGCGAGATGAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACGATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGATTGGCGA  >  CP009273/3954215‑3954512
                                                                                                                                                     |                                                                                                                                                    
CGCGCGAGATGAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACT                                                                                                                                                      <  SRR19973065.3486927/150‑1 (MQ=60)
    CGAGATGAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATAC                                                                                                                                                  <  SRR19973065.3049585/150‑1 (MQ=60)
    CGAGATGAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATAC                                                                                                                                                  <  SRR19973065.2718456/150‑1 (MQ=60)
     GAGATGAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACC                                                                                                                                                 >  SRR19973065.986332/1‑150 (MQ=60)
     GAGATGAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACC                                                                                                                                                 <  SRR19973065.3510294/150‑1 (MQ=60)
         TGAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATC                                                                                                                                             <  SRR19973065.2985992/150‑1 (MQ=60)
          GAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCA                                                                                                                                            <  SRR19973065.2666121/150‑1 (MQ=60)
           AAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAG                                                                                                                                           <  SRR19973065.2900998/150‑1 (MQ=60)
           AAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAG                                                                                                                                           >  SRR19973065.3135397/1‑150 (MQ=60)
            AAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGC                                                                                                                                          >  SRR19973065.1893932/1‑150 (MQ=60)
             AGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCT                                                                                                                                         <  SRR19973065.1746797/150‑1 (MQ=60)
               AGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTG                                                                                                                                       <  SRR19973065.1027348/150‑1 (MQ=60)
               AGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTG                                                                                                                                       >  SRR19973065.803766/1‑150 (MQ=60)
                   TGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTT                                                                                                                                   <  SRR19973065.628893/150‑1 (MQ=60)
                      AGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCT                                                                                                                                >  SRR19973065.936360/1‑150 (MQ=60)
                           AGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAG                                                                                                                           >  SRR19973065.1815456/1‑150 (MQ=60)
                             ACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAG                                                                                                                         <  SRR19973065.3372251/150‑1 (MQ=60)
                             ACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAG                                                                                                                         <  SRR19973065.3372253/150‑1 (MQ=60)
                             ACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAG                                                                                                                         <  SRR19973065.3372460/150‑1 (MQ=60)
                              CGCTGGGGCGCATAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGT                                                                                                                        <  SRR19973065.451657/150‑1 (MQ=60)
                                 TGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGA                                                                                                                     >  SRR19973065.2516523/1‑150 (MQ=60)
                                 TGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGA                                                                                                                     >  SRR19973065.500656/1‑150 (MQ=60)
                                   GGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACC                                                                                                                   >  SRR19973065.915001/1‑150 (MQ=60)
                                   GGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACC                                                                                                                   >  SRR19973065.2434649/1‑150 (MQ=60)
                                   GGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACC                                                                                                                   >  SRR19973065.2434203/1‑150 (MQ=60)
                                   GGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACC                                                                                                                   <  SRR19973065.1379950/150‑1 (MQ=60)
                                   GGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACC                                                                                                                   <  SRR19973065.1658454/150‑1 (MQ=60)
                                        CAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGG                                                                                                              <  SRR19973065.3335909/150‑1 (MQ=60)
                                        CAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGG                                                                                                              <  SRR19973065.178170/150‑1 (MQ=60)
                                         AAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGG                                                                                                             >  SRR19973065.976176/1‑150 (MQ=60)
                                         AAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGG                                                                                                             >  SRR19973065.975161/1‑150 (MQ=60)
                                           AGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGATTTGACCGAGGGGCT                                                                                                           >  SRR19973065.1681845/1‑150 (MQ=60)
                                           AGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCT                                                                                                           >  SRR19973065.1538735/1‑150 (MQ=60)
                                           AGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCT                                                                                                           <  SRR19973065.3244535/150‑1 (MQ=60)
                                           AGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCT                                                                                                           <  SRR19973065.317221/150‑1 (MQ=60)
                                           AGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCT                                                                                                           <  SRR19973065.910609/150‑1 (MQ=60)
                                              GGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGAT                                                                                                        <  SRR19973065.3098714/150‑1 (MQ=60)
                                                    TGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGA                                                                                                  <  SRR19973065.1282195/150‑1 (MQ=60)
                                                    TGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGA                                                                                                  <  SRR19973065.1282154/150‑1 (MQ=60)
                                                     GGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGAT                                                                                                 >  SRR19973065.1610535/1‑150 (MQ=60)
                                                         TGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACA                                                                                             <  SRR19973065.791755/150‑1 (MQ=60)
                                                          GATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAA                                                                                            >  SRR19973065.1128050/1‑150 (MQ=60)
                                                          GATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAA                                                                                            >  SRR19973065.2473454/1‑150 (MQ=60)
                                                           ATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGAATGCGGCGCTGGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTTACCGAGGGGCTGATTGAAGATGACAAA                                                                                           >  SRR19973065.1798900/1‑150 (MQ=60)
                                                              ATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCNCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTT                                                                                        >  SRR19973065.1794920/1‑150 (MQ=60)
                                                                   CACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCT                                                                                   >  SRR19973065.132830/1‑150 (MQ=60)
                                                                    ACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTG                                                                                  >  SRR19973065.1125021/1‑150 (MQ=60)
                                                                    ACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTG                                                                                  <  SRR19973065.3319969/150‑1 (MQ=60)
                                                                           ATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAAC                                                                           <  SRR19973065.2467675/150‑1 (MQ=60)
                                                                            TACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACT                                                                          <  SRR19973065.1710931/150‑1 (MQ=60)
                                                                                TTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATT                                                                      <  SRR19973065.1914037/150‑1 (MQ=60)
                                                                                TTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATT                                                                      <  SRR19973065.2796075/150‑1 (MQ=60)
                                                                                 TGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTT                                                                     >  SRR19973065.170659/1‑150 (MQ=60)
                                                                                       TGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCA                                                               >  SRR19973065.1584663/1‑150 (MQ=60)
                                                                                        GGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCAT                                                              <  SRR19973065.729159/150‑1 (MQ=60)
                                                                                          ATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCT                                                            >  SRR19973065.424888/1‑150 (MQ=60)
                                                                                          ATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCT                                                            >  SRR19973065.2206046/1‑150 (MQ=60)
                                                                                            ATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCT                                                          >  SRR19973065.370164/1‑150 (MQ=60)
                                                                                            ATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCT                                                          >  SRR19973065.2333692/1‑150 (MQ=60)
                                                                                            ATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCT                                                          >  SRR19973065.2334333/1‑150 (MQ=60)
                                                                                               ATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAAC                                                       <  SRR19973065.3141739/150‑1 (MQ=60)
                                                                                               ATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAAC                                                       <  SRR19973065.3141053/150‑1 (MQ=60)
                                                                                               ATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAAGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAAC                                                       <  SRR19973065.1698092/150‑1 (MQ=60)
                                                                                                 CAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTG                                                     >  SRR19973065.3229581/1‑150 (MQ=60)
                                                                                                  AAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGG                                                    >  SRR19973065.1432211/1‑150 (MQ=60)
                                                                                                   AACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGA                                                   <  SRR19973065.3409894/150‑1 (MQ=60)
                                                                                                   AACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGA                                                   <  SRR19973065.931369/150‑1 (MQ=60)
                                                                                                     CGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAATATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAG                                                 >  SRR19973065.2385793/1‑150 (MQ=60)
                                                                                                     CGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAG                                                 <  SRR19973065.562191/150‑1 (MQ=60)
                                                                                                     CGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAG                                                 <  SRR19973065.2451802/150‑1 (MQ=60)
                                                                                                     CGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAG                                                 <  SRR19973065.1753621/150‑1 (MQ=60)
                                                                                                         AGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATG                                             >  SRR19973065.2987852/1‑150 (MQ=60)
                                                                                                         AGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATG                                             >  SRR19973065.2987680/1‑150 (MQ=60)
                                                                                                          GTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTTGAAGAATGA                                            >  SRR19973065.371797/1‑150 (MQ=60)
                                                                                                            ATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATC                                          >  SRR19973065.1429327/1‑150 (MQ=60)
                                                                                                            ATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATC                                          <  SRR19973065.1355147/150‑1 (MQ=60)
                                                                                                            ATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATC                                          <  SRR19973065.3267039/150‑1 (MQ=60)
                                                                                                            ATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATC                                          <  SRR19973065.3266831/150‑1 (MQ=60)
                                                                                                            ATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATC                                          >  SRR19973065.1426488/1‑150 (MQ=60)
                                                                                                              GCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTC                                        <  SRR19973065.2046945/150‑1 (MQ=60)
                                                                                                               CGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCA                                       <  SRR19973065.577172/150‑1 (MQ=60)
                                                                                                               CGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCA                                       >  SRR19973065.3509021/1‑150 (MQ=60)
                                                                                                               CGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCA                                       <  SRR19973065.2991304/150‑1 (MQ=60)
                                                                                                               CGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCA                                       <  SRR19973065.2351366/150‑1 (MQ=60)
                                                                                                                    CTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACA                                  <  SRR19973065.2013102/150‑1 (MQ=60)
                                                                                                                    CTTGGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACA                                  <  SRR19973065.2013455/150‑1 (MQ=60)
                                                                                                                       GGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCG                               >  SRR19973065.494648/1‑150 (MQ=60)
                                                                                                                       GGGATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCG                               <  SRR19973065.1848643/150‑1 (MQ=60)
                                                                                                                         GATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTC                             <  SRR19973065.629317/150‑1 (MQ=60)
                                                                                                                          ATGAAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCC                            >  SRR19973065.3202738/1‑150 (MQ=60)
                                                                                                                             AAGGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAG                         >  SRR19973065.2998665/1‑150 (MQ=60)
                                                                                                                             AAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAG                         >  SRR19973065.1276870/1‑150 (MQ=60)
                                                                                                                             AAAGCGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAG                         >  SRR19973065.2998548/1‑150 (MQ=60)
                                                                                                                                 CGAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGG                     <  SRR19973065.2724155/150‑1 (MQ=60)
                                                                                                                                  GAACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGC                    >  SRR19973065.692214/1‑150 (MQ=60)
                                                                                                                                   AACTTCTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCA                   >  SRR19973065.1086709/1‑150 (MQ=60)
                                                                                                                                        CTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTGAAAACACCGTCCCAGGCGGCAGCAAG              >  SRR19973065.1096424/1‑150 (MQ=60)
                                                                                                                                        CTCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAG              >  SRR19973065.2516332/1‑150 (MQ=60)
                                                                                                                                         TCGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGT             <  SRR19973065.3111238/150‑1 (MQ=60)
                                                                                                                                          CGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTG            <  SRR19973065.3166025/150‑1 (MQ=60)
                                                                                                                                          CGTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTG            >  SRR19973065.2988769/1‑150 (MQ=60)
                                                                                                                                           GTTGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGC           >  SRR19973065.2537542/1‑150 (MQ=60)
                                                                                                                                             TGTTTGACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGA         >  SRR19973065.244537/1‑150 (MQ=60)
                                                                                                                                                TTGACTATACAGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGATTG      <  SRR19973065.3250183/150‑1 (MQ=60)
                                                                                                                                                  GACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGATTGGC    <  SRR19973065.3021254/150‑1 (MQ=60)
                                                                                                                                                   ACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGATTGGCG   >  SRR19973065.2998776/1‑150 (MQ=60)
                                                                                                                                                   ACTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGATTGGCG   >  SRR19973065.2839577/1‑150 (MQ=60)
                                                                                                                                                    CTATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGATTGGCGA  >  SRR19973065.2771537/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CGCGCGAGATGAAAGAGCGTGTAGGGCAGACGCTGGGGCGCAAAGAGGCGCGTGGGCTGATGATCTCCACTTTCCATACGTTGGGGCTGGATATCATCAAACGCGAGTATGCGGCGCTTGGGATGAAAGCGAACTTCTCGTTGTTTGACGATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGATTGGCGA  >  CP009273/3954215‑3954512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑