Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,219,790 A→T 100% L94Q (CTG→CAG)  pepE ← (alpha)‑aspartyl dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,219,7900AT98.6% 683.5 / ‑0.4 208L94Q (CTG→CAG) pepE(alpha)‑aspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base A (3/0);  new base T (82/123);  total (85/123)
Fisher's exact test for biased strand distribution p-value = 6.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 208 total aligned reads displayed.

GCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCC  >  CP009273/4219647‑4219933
                                                                                                                                               |                                                                                                                                               
gCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCaa                                                                                                                                             <  1:2646714/148‑1 (MQ=255)
  ggATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACt                                                                                                                                           <  1:3457831/148‑1 (MQ=255)
   gATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTg                                                                                                                                          >  1:504827/1‑148 (MQ=255)
   gATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTg                                                                                                                                          >  1:3113662/1‑148 (MQ=255)
   gATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTg                                                                                                                                          >  2:3461884/1‑148 (MQ=255)
      cGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGaa                                                                                                                                       >  2:1835751/1‑148 (MQ=255)
       gACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGaaa                                                                                                                                      >  1:2789578/1‑148 (MQ=255)
         cAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGt                                                                                                                                    <  2:2880110/148‑1 (MQ=255)
         cAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGt                                                                                                                                    <  1:2333771/148‑1 (MQ=255)
          aaTCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTa                                                                                                                                   >  1:1028721/1‑148 (MQ=255)
          aaTCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTa                                                                                                                                   >  2:506580/1‑148 (MQ=255)
           aTCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTAt                                                                                                                                  <  2:1376848/148‑1 (MQ=255)
           aTCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTAt                                                                                                                                  >  2:1888213/1‑148 (MQ=255)
            tCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTAtt                                                                                                                                 >  2:3349668/1‑148 (MQ=255)
            tCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTAtt                                                                                                                                 >  1:3400606/1‑148 (MQ=255)
             cGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTc                                                                                                                                <  1:1699951/148‑1 (MQ=255)
              ggCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTcc                                                                                                                               <  2:2375036/148‑1 (MQ=255)
                cATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCccg                                                                                                                             <  2:2731765/148‑1 (MQ=255)
                cATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCccg                                                                                                                             <  2:2750843/148‑1 (MQ=255)
                   atCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCccgccg                                                                                                                          >  2:1687773/1‑148 (MQ=255)
                   atCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCccgccg                                                                                                                          >  1:3331784/1‑148 (MQ=255)
                    tCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCccgccgc                                                                                                                         <  2:786403/148‑1 (MQ=255)
                    tCGTTGGTGGTACGAATAGGTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCccgccgc                                                                                                                         <  2:2602595/148‑1 (MQ=255)
                          gtggtACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACaa                                                                                                                   <  1:48670/148‑1 (MQ=255)
                            ggtACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATc                                                                                                                 <  1:1989544/148‑1 (MQ=255)
                               aCGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACg                                                                                                              >  2:1443273/1‑148 (MQ=255)
                                cGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGa                                                                                                             <  1:2754463/148‑1 (MQ=255)
                                  aaTAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATc                                                                                                                 >  1:1293099/1‑142 (MQ=255)
                                  aaTAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGAtc                                                                                                           >  2:1828825/1‑148 (MQ=255)
                                  aaTAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGAtc                                                                                                           >  2:2086705/1‑148 (MQ=255)
                                    tAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGAtctc                                                                                                         >  1:2887730/1‑148 (MQ=255)
                                      gTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAg                                                                                                       <  2:639413/148‑1 (MQ=255)
                                      gTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAg                                                                                                       <  2:2402007/148‑1 (MQ=255)
                                       ttGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGc                                                                                                      >  1:2574372/1‑148 (MQ=255)
                                        tGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCa                                                                                                     <  1:24186/148‑1 (MQ=255)
                                          ggCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCAtt                                                                                                   <  1:2377419/148‑1 (MQ=255)
                                            caagcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCAtttt                                                                                                 <  1:704815/148‑1 (MQ=255)
                                               gcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCaa                                                                                              <  1:110747/148‑1 (MQ=255)
                                               gcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCaa                                                                                              <  1:2115336/148‑1 (MQ=255)
                                                caagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAAt                                                                                             >  1:1209569/1‑148 (MQ=255)
                                                caagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAAt                                                                                             <  2:767684/148‑1 (MQ=255)
                                                caagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAAt                                                                                             <  2:400370/148‑1 (MQ=255)
                                                caagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAAt                                                                                             <  2:1201743/148‑1 (MQ=255)
                                                     ttAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCgg                                                                                        <  1:3269911/148‑1 (MQ=255)
                                                      tAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGc                                                                                       <  2:253845/148‑1 (MQ=255)
                                                      tAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGc                                                                                       >  2:2788383/1‑148 (MQ=255)
                                                        gcgcCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCaa                                                                                     <  2:1484714/148‑1 (MQ=255)
                                                          gcCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACg                                                                                   <  1:2263038/148‑1 (MQ=255)
                                                          gcCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACg                                                                                   <  2:1615558/148‑1 (MQ=255)
                                                            cTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACggg                                                                                 <  1:1888213/148‑1 (MQ=255)
                                                             tGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGa                                                                                <  2:3203744/148‑1 (MQ=255)
                                                               cgcTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATc                                                                              <  1:543328/148‑1 (MQ=255)
                                                                    cAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACaa                                                                         <  1:1752053/148‑1 (MQ=255)
                                                                    cAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACaa                                                                         <  2:2821691/148‑1 (MQ=255)
                                                                      gCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACg                                                                       <  2:871604/148‑1 (MQ=255)
                                                                      gCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACg                                                                       <  1:2314502/148‑1 (MQ=255)
                                                                      gCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACg                                                                       >  1:98950/1‑148 (MQ=255)
                                                                        cAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCt                                                                     >  1:2871821/1‑148 (MQ=255)
                                                                        cAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCt                                                                     <  1:115934/148‑1 (MQ=255)
                                                                        cAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCt                                                                     <  2:2129229/148‑1 (MQ=255)
                                                                         aaTATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTa                                                                    <  1:1749799/148‑1 (MQ=255)
                                                                          atatACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTAt                                                                   <  2:3260496/148‑1 (MQ=255)
                                                                           tataCAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATg                                                                  >  1:1486093/1‑148 (MQ=255)
                                                                              aCAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAAt                                                               >  2:365398/1‑148 (MQ=255)
                                                                                 gagCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATAcc                                                            >  1:988688/1‑148 (MQ=255)
                                                                                 gagCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATAcc                                                            >  1:993094/1‑148 (MQ=255)
                                                                                         cGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAg                                                    <  2:1534718/148‑1 (MQ=255)
                                                                                          gTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGa                                                   <  2:2895864/148‑1 (MQ=255)
                                                                                             tAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAac                                                <  1:3230152/148‑1 (MQ=255)
                                                                                                  aCGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCa                                           >  2:1098765/1‑148 (MQ=255)
                                                                                                     tCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCTCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCg                                        >  1:1419374/1‑148 (MQ=255)
                                                                                                      cAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCgg                                       >  2:2237178/1‑148 (MQ=255)
                                                                                                      cAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCgg                                       >  2:2962637/1‑148 (MQ=255)
                                                                                                      cAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCgg                                       <  2:1320921/148‑1 (MQ=255)
                                                                                                        gTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCACTCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAg                                     <  1:226667/148‑1 (MQ=255)
                                                                                                        gTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAg                                     <  2:2010556/148‑1 (MQ=255)
                                                                                                        gTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAg                                     >  1:879353/1‑148 (MQ=255)
                                                                                                          aaTTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCg                                   <  2:879353/148‑1 (MQ=255)
                                                                                                          aaTTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCg                                   >  2:3117829/1‑148 (MQ=255)
                                                                                                          aaTTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCg                                   <  1:94895/148‑1 (MQ=255)
                                                                                                                   cagcagCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCa                          >  1:1318300/1‑148 (MQ=255)
                                                                                                                    agcagcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAg                         <  2:1486093/148‑1 (MQ=255)
                                                                                                                    agcagcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAg                         <  1:2938808/148‑1 (MQ=255)
                                                                                                                     gcagcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGc                        >  1:1099993/1‑148 (MQ=255)
                                                                                                                      cagcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGcc                       >  1:383513/1‑148 (MQ=255)
                                                                                                                      cagcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGcc                       >  2:598848/1‑148 (MQ=255)
                                                                                                                        gcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGt                     >  1:2861504/1‑148 (MQ=255)
                                                                                                                         cccGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGtt                    <  2:1135697/148‑1 (MQ=255)
                                                                                                                          ccGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGttt                   <  2:3400606/148‑1 (MQ=255)
                                                                                                                           cgcgCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGtttt                  <  1:3018524/148‑1 (MQ=255)
                                                                                                                              gcTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGc               <  2:1526032/148‑1 (MQ=255)
                                                                                                                               cTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCt              <  1:1835751/148‑1 (MQ=255)
                                                                                                                                tCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCtg             <  2:540870/148‑1 (MQ=255)
                                                                                                                                  gcgGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCtgtg           <  2:3383538/148‑1 (MQ=255)
                                                                                                                                      cACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAat       <  1:2257777/148‑1 (MQ=255)
                                                                                                                                       aCTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatc      <  2:1902600/148‑1 (MQ=255)
                                                                                                                                        cTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatca     >  1:1921184/1‑148 (MQ=255)
                                                                                                                                         tGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTaataat    >  2:3424872/1‑148 (MQ=255)
                                                                                                                                           tttCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATcc  <  2:2215536/148‑1 (MQ=255)
                                                                                                                                           tttCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATcc  <  1:2509542/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCC  >  CP009273/4219647‑4219933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGT  >  CP009273/4219641‑4219938
                                                                                                                                                     |                                                                                                                                                     
AACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCT                                                                                                                                                       <  SRR19973065.1091363/150‑1 (MQ=60)
 ACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTG                                                                                                                                                      <  SRR19973065.3487715/150‑1 (MQ=60)
      GCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACT                                                                                                                                                 <  SRR19973065.2663030/150‑1 (MQ=60)
       CGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTG                                                                                                                                                >  SRR19973065.508196/1‑150 (MQ=60)
       CGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTG                                                                                                                                                >  SRR19973065.3132640/1‑150 (MQ=60)
        GGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGG                                                                                                                                               <  SRR19973065.3478902/150‑1 (MQ=60)
         GATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGA                                                                                                                                              >  SRR19973065.2893951/1‑150 (MQ=60)
           TCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAA                                                                                                                                            <  SRR19973065.1801329/150‑1 (MQ=60)
           TCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAA                                                                                                                                            >  SRR19973065.1198975/1‑150 (MQ=60)
           TCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAA                                                                                                                                            >  SRR19973065.2806868/1‑150 (MQ=60)
            CGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAG                                                                                                                                           >  SRR19973065.1090064/1‑150 (MQ=60)
             GACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGT                                                                                                                                          >  SRR19973065.3380134/1‑150 (MQ=60)
              ACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTA                                                                                                                                         >  SRR19973065.1035533/1‑150 (MQ=60)
               CAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTAT                                                                                                                                        <  SRR19973065.3520819/150‑1 (MQ=60)
               CAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTAT                                                                                                                                        <  SRR19973065.2348353/150‑1 (MQ=60)
                AATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATT                                                                                                                                       >  SRR19973065.3421390/1‑150 (MQ=60)
                 ATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTC                                                                                                                                      <  SRR19973065.2942506/150‑1 (MQ=60)
                   CGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCC                                                                                                                                    <  SRR19973065.1296161/150‑1 (MQ=60)
                   CGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCC                                                                                                                                    <  SRR19973065.1893786/150‑1 (MQ=60)
                   CGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCC                                                                                                                                    <  SRR19973065.1711285/150‑1 (MQ=60)
                    GGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCG                                                                                                                                   >  SRR19973065.2839121/1‑150 (MQ=60)
                      CATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCC                                                                                                                                 >  SRR19973065.3404227/1‑150 (MQ=60)
                       ATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCG                                                                                                                                >  SRR19973065.3352105/1‑150 (MQ=60)
                       ATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCG                                                                                                                                >  SRR19973065.3352121/1‑150 (MQ=60)
                                GTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATC                                                                                                                       <  SRR19973065.49224/150‑1 (MQ=60)
                                GTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATC                                                                                                                       <  SRR19973065.49244/150‑1 (MQ=60)
                                GTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATC                                                                                                                       <  SRR19973065.277525/150‑1 (MQ=60)
                                  GGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCATTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC                                                                                                                     <  SRR19973065.2334685/150‑1 (MQ=60)
                                  GGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC                                                                                                                     <  SRR19973065.2002452/150‑1 (MQ=60)
                                  GGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC                                                                                                                     <  SRR19973065.216429/150‑1 (MQ=60)
                                  GGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC                                                                                                                     <  SRR19973065.556019/150‑1 (MQ=60)
                                      CGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACAGAT                                                                                                                 >  SRR19973065.1301618/1‑150 (MQ=60)
                                      CGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATC                                                                                                                <  SRR19973065.2771599/150‑1 (MQ=60)
                                       GAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCT                                                                                                               <  SRR19973065.1581035/150‑1 (MQ=60)
                                        AATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTC                                                                                                              >  SRR19973065.2905386/1‑150 (MQ=60)
                                         ATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCA                                                                                                             <  SRR19973065.271022/150‑1 (MQ=60)
                                          TAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAG                                                                                                            <  SRR19973065.903778/150‑1 (MQ=60)
                                           AGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGC                                                                                                           >  SRR19973065.2507333/1‑150 (MQ=60)
                                           AGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGC                                                                                                           >  SRR19973065.2590181/1‑150 (MQ=60)
                                           AGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGC                                                                                                           >  SRR19973065.2590433/1‑150 (MQ=60)
                                              TGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATT                                                                                                        <  SRR19973065.24508/150‑1 (MQ=60)
                                                GGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTT                                                                                                      <  SRR19973065.2392276/150‑1 (MQ=60)
                                                 GCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTC                                                                                                     <  SRR19973065.188581/150‑1 (MQ=60)
                                                  CAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCA                                                                                                    <  SRR19973065.709505/150‑1 (MQ=60)
                                                  CAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCA                                                                                                    >  SRR19973065.2279319/1‑150 (MQ=60)
                                                  CAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCA                                                                                                    <  SRR19973065.237773/150‑1 (MQ=60)
                                                    AGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAAT                                                                                                  >  SRR19973065.1217368/1‑150 (MQ=60)
                                                     GCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATC                                                                                                 <  SRR19973065.2128948/150‑1 (MQ=60)
                                                     GCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATC                                                                                                 <  SRR19973065.111740/150‑1 (MQ=60)
                                                     GCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATC                                                                                                 <  SRR19973065.111747/150‑1 (MQ=60)
                                                       AAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGC                                                                                               >  SRR19973065.311122/1‑150 (MQ=60)
                                                           TTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCA                                                                                           <  SRR19973065.3289744/150‑1 (MQ=60)
                                                                GCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGG                                                                                      <  SRR19973065.2277343/150‑1 (MQ=60)
                                                                 CCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGA                                                                                     >  SRR19973065.2284577/1‑150 (MQ=60)
                                                                  CTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGAT                                                                                    <  SRR19973065.1901133/150‑1 (MQ=60)
                                                                  CTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGAT                                                                                    <  SRR19973065.1900632/150‑1 (MQ=60)
                                                                    GCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCT                                                                                  <  SRR19973065.3246524/150‑1 (MQ=60)
                                                                    GCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCT                                                                                  <  SRR19973065.1153623/150‑1 (MQ=60)
                                                                     CGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTA                                                                                 <  SRR19973065.546663/150‑1 (MQ=60)
                                                                     CGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTA                                                                                 <  SRR19973065.546847/150‑1 (MQ=60)
                                                                       CTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACA                                                                               <  SRR19973065.963477/150‑1 (MQ=60)
                                                                          CAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACG                                                                            <  SRR19973065.1762367/150‑1 (MQ=60)
                                                                          CAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACG                                                                            <  SRR19973065.1763586/150‑1 (MQ=60)
                                                                          CAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACG                                                                            >  SRR19973065.99843/1‑150 (MQ=60)
                                                                          CAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACG                                                                            >  SRR19973065.99858/1‑150 (MQ=60)
                                                                           AGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGC                                                                           >  SRR19973065.3471661/1‑150 (MQ=60)
                                                                            GCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCT                                                                          >  SRR19973065.2889371/1‑150 (MQ=60)
                                                                            GCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCT                                                                          >  SRR19973065.2889421/1‑150 (MQ=60)
                                                                            GCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCT                                                                          <  SRR19973065.2329016/150‑1 (MQ=60)
                                                                              CAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTAT                                                                        <  SRR19973065.2277163/150‑1 (MQ=60)
                                                                              CAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTAT                                                                        <  SRR19973065.116954/150‑1 (MQ=60)
                                                                               AATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATG                                                                       >  SRR19973065.1495576/1‑150 (MQ=60)
                                                                               AATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATG                                                                       <  SRR19973065.1761326/150‑1 (MQ=60)
                                                                                     CAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACC                                                                 >  SRR19973065.995256/1‑150 (MQ=60)
                                                                                     CAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACC                                                                 >  SRR19973065.999688/1‑150 (MQ=60)
                                                                                     CAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACC                                                                 >  SRR19973065.999694/1‑150 (MQ=60)
                                                                                      AGAGCGCCACGTTTAACCACGTCAGTAATTGGTTCCAGCATCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATACCCGCCGCCGACAATCAC‑GATCTCAGCATTATCAATCTCGGCAACGGGATCTACAACGCTCTGAATACCG                                                                >  SRR19973065.1943586/1‑150 (MQ=60)
                                                                                      AGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCG                                                                >  SRR19973065.2398883/1‑150 (MQ=60)
                                                                                        AGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGT                                                              <  SRR19973065.1452613/150‑1 (MQ=60)
                                                                                                   TAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACAC                                                   <  SRR19973065.3249549/150‑1 (MQ=60)
                                                                                                         CGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCTCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCG                                             >  SRR19973065.1428639/1‑150 (MQ=60)
                                                                                                            CAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAG                                          >  SRR19973065.884956/1‑150 (MQ=60)
                                                                                                              GTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCACTCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCG                                        <  SRR19973065.228329/150‑1 (MQ=60)
                                                                                                                AATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAG                                      <  SRR19973065.95785/150‑1 (MQ=60)
                                                                                                                      TGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGC                                >  SRR19973065.434259/1‑150 (MQ=60)
                                                                                                                       GCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCA                               >  SRR19973065.1326091/1‑150 (MQ=60)
                                                                                                                       GCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCA                               >  SRR19973065.1327020/1‑150 (MQ=60)
                                                                                                                         CAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGC                             >  SRR19973065.1107223/1‑150 (MQ=60)
                                                                                                                         CAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGC                             >  SRR19973065.2984676/1‑150 (MQ=60)
                                                                                                                          AGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCC                            >  SRR19973065.386194/1‑150 (MQ=60)
                                                                                                                          AGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCC                            <  SRR19973065.2956639/150‑1 (MQ=60)
                                                                                                                            CAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGT                          >  SRR19973065.2879073/1‑150 (MQ=60)
                                                                                                                            CAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGT                          >  SRR19973065.2620884/1‑150 (MQ=60)
                                                                                                                             AGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTT                         >  SRR19973065.870680/1‑150 (MQ=60)
                                                                                                                                 CGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCG                     <  SRR19973065.3037018/150‑1 (MQ=60)
                                                                                                                                    GCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTG                  <  SRR19973065.1719233/150‑1 (MQ=60)
                                                                                                                                     CTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGT                 <  SRR19973065.1847856/150‑1 (MQ=60)
                                                                                                                                        GCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTA              <  SRR19973065.1387863/150‑1 (MQ=60)
                                                                                                                                          GGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAAT            <  SRR19973065.3274185/150‑1 (MQ=60)
                                                                                                                                            CACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCA          >  SRR19973065.1933784/1‑150 (MQ=60)
                                                                                                                                            CACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCA          <  SRR19973065.2272065/150‑1 (MQ=60)
                                                                                                                                               TGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCC       <  SRR19973065.2102041/150‑1 (MQ=60)
                                                                                                                                                 TTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCA     <  SRR19973065.2525480/150‑1 (MQ=60)
                                                                                                                                                 TTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCA     <  SRR19973065.2525313/150‑1 (MQ=60)
                                                                                                                                                   TCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGG   >  SRR19973065.1489339/1‑150 (MQ=60)
                                                                                                                                                   TCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTGTTCGCTGTGTAATCATCCCAGG   >  SRR19973065.1489112/1‑150 (MQ=60)
                                                                                                                                                    CTGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGT  >  SRR19973065.1280691/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCAC‑GATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGT  >  CP009273/4219641‑4219938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑