Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 522,859 T→G 19.1% S1381R (AGT→AGG)  rhsD → Rhs family putative polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273522,8590TG19.1% 290.1 / 77.1 135S1381R (AGT→AGG) rhsDRhs family putative polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base T (52/56);  new base G (10/16);  total (63/72)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

ATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCT  >  CP009273/522711‑523007
                                                                                                                                                    |                                                                                                                                                    
aTAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTa                                                                                                                                                     >  2:1242541/1‑150 (MQ=255)
   aTTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAg                                                                                                                                                  <  1:1493562/150‑1 (MQ=255)
    tttAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGa                                                                                                                                                 >  2:4276350/1‑150 (MQ=255)
        aGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGataat                                                                                                                                             >  1:1313113/1‑150 (MQ=255)
        aGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGataat                                                                                                                                             <  1:82315/150‑1 (MQ=255)
        aGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGGAAAGataat                                                                                                                                             >  2:4499102/1‑150 (MQ=255)
         gAAATGAGTCATATCCTCTTAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGataata                                                                                                                                            <  2:1292558/150‑1 (MQ=255)
         gAAAGGTGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCTTGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGataata                                                                                                                                            <  2:3636467/150‑1 (MQ=255)
          aaaGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGAtaataa                                                                                                                                           >  2:1383120/1‑150 (MQ=255)
           aaGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATaataat                                                                                                                                          <  2:1183952/150‑1 (MQ=255)
           aaGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATaataat                                                                                                                                          <  2:1185491/150‑1 (MQ=255)
              gAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTATCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCtt                                                                                                                                       >  2:2068598/1‑150 (MQ=255)
              gAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCtt                                                                                                                                       >  2:753766/1‑150 (MQ=255)
              gAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCtt                                                                                                                                       >  2:4471223/1‑150 (MQ=255)
               aGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTg                                                                                                                                      >  2:1876274/1‑150 (MQ=255)
               aGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTg                                                                                                                                      <  1:3663388/150‑1 (MQ=255)
               aGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTg                                                                                                                                      <  1:3663432/150‑1 (MQ=255)
               aGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTg                                                                                                                                      <  1:3664317/150‑1 (MQ=255)
                  cATATACTCTTAAAATAAAATGTCCCACATGTACAGATATATTTGATTCTTATCATACTAACGGTTAATATAGTCATGTCTATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGGAAAGATAATAATCTTGAAg                                                                                                                                   <  2:4235118/150‑1 (MQ=255)
                   atatCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGc                                                                                                                                  >  2:1459916/1‑150 (MQ=255)
                   atatCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGGAAAGATAATAATCTTGAAGc                                                                                                                                  >  1:3531662/1‑150 (MQ=255)
                   atatCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTAAAGTAGCGATAAAAATATTGTAAGAAGTAAAGATAATAATCTTGAAGc                                                                                                                                  >  1:3221513/1‑150 (MQ=255)
                    tatCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCa                                                                                                                                 >  2:2315831/1‑150 (MQ=255)
                     atCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGTAGTTAAGATAATAATCTTGAAGCAt                                                                                                                                >  1:407782/1‑150 (MQ=255)
                     atCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTAAAGTAGCGATAAAAATATTGTAAGAAGGAAAGATAATAATCTTGAAGCAt                                                                                                                                >  1:3232859/1‑150 (MQ=255)
                       ccTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAATAAGGAAAGATAATAATATTGAATCtttt                                                                                                                              >  2:2503891/1‑150 (MQ=255)
                       ccTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCAttt                                                                                                                              <  1:302918/150‑1 (MQ=255)
                       ccTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCAttt                                                                                                                              <  1:302637/150‑1 (MQ=255)
                           tGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGTTGAATTTTTTTCAAGTAGCGATAAAAATATTGTAAGAAGGAAATATAATAATATTGAAGCATTTTAta                                                                                                                          >  1:845413/1‑149 (MQ=255)
                           tGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTAtc                                                                                                                          <  1:1921581/150‑1 (MQ=255)
                           tGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTAAATTAGCGATAAAAATCTTGTAAGAAGGAAAGATAATAATATTTAATCATTTTAta                                                                                                                          >  1:847096/1‑149 (MQ=255)
                            gaaaagaaaaTGTCCCACATTTACAGATAGATTTTCTTCTTATCATACTCACGGTGCAGATATTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTAtct                                                                                                                         <  2:2844513/150‑1 (MQ=255)
                               aagaaaaTGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGc                                                                                                                      >  1:1352732/1‑150 (MQ=255)
                                 gaaaaTGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATAATCACGGTGCAGATAGTAATGGCGATTATTTTGATGAAATTTTTTAAAGTATCGATAAAAATCTTGTAAGAAGAAAATAACATAATCTTGAATCATTTAATCTCGCaa                                                                                                                    >  1:77442/1‑150 (MQ=255)
                                   aaaTGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAaca                                                                                                                  >  2:2389985/1‑150 (MQ=255)
                                   aaaTGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGGAAAGATAATAATCTTGAAGCATTTTATCTCGCAaca                                                                                                                  <  1:4313078/150‑1 (MQ=255)
                                     aTGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAATTAGCGATAAAAATCTTGTAAGAAGGAAAGATAATAATCTTGAATCATTTTATCTCGCAACAcc                                                                                                                >  1:2426937/1‑150 (MQ=255)
                                      tGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCt                                                                                                               >  2:3769241/1‑150 (MQ=255)
                                      tGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCt                                                                                                               <  1:1940599/150‑1 (MQ=255)
                                      tGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATATTGTTAGAAGTAAAGATAATAATCTTGAATCATTTTATCTCGCAACACCt                                                                                                               >  2:1620905/1‑150 (MQ=255)
                                        tCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGa                                                                                                             >  2:3964382/1‑150 (MQ=255)
                                        tCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGa                                                                                                             >  2:3964418/1‑150 (MQ=255)
                                         cccACAGGTACAGATAGAGTTGCTGATTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGGAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGAt                                                                                                            <  2:1223656/150‑1 (MQ=255)
                                          ccACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATg                                                                                                           <  1:2716999/150‑1 (MQ=255)
                                            acaGGTACATATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGa                                                                                                         <  2:3037869/150‑1 (MQ=255)
                                            acaGGTACAGATATTGTTGATTCTTATCATACTCACGGTTCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGa                                                                                                         <  2:3037842/150‑1 (MQ=255)
                                               ggTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAAACTTGTAAGAAGTAAAGATAATAATCTTTAAGCATTTTATCTCGCAACACCTGATGGACGa                                                                                                      >  1:2400440/1‑150 (MQ=255)
                                                    aGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAATTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTTAAGCATTTTATATCGCAACACCTGATGGACGATTTGa                                                                                                 >  1:2943924/1‑150 (MQ=255)
                                                     gATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAg                                                                                                >  2:1980925/1‑150 (MQ=255)
                                                     gATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAg                                                                                                >  2:4382361/1‑150 (MQ=255)
                                                        agagTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGgcg                                                                                             <  1:4685991/150‑1 (MQ=255)
                                                        agagTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGgcg                                                                                             <  1:4685691/150‑1 (MQ=255)
                                                        agagTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGGAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGgcg                                                                                             <  2:2577099/150‑1 (MQ=255)
                                                           gTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCtt                                                                                          <  1:3950501/150‑1 (MQ=255)
                                                             ttctgctTATCATACTCTCGGTGCAGATATTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTtaa                                                                                        <  2:108588/148‑1 (MQ=255)
                                                              gcttctTATCATACTCACTGTGCAGATAGTCATGGCGATTATTTTGATGAATTTTTTTCAAGTATCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTtaat                                                                                       <  2:1267495/150‑1 (MQ=255)
                                                               ctgctTATCATACTCACGGTGCAGATAGACATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTtaata                                                                                      >  2:3364551/1‑150 (MQ=255)
                                                                tgctTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTtaataa                                                                                     >  2:1516311/1‑150 (MQ=255)
                                                                 gctTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTtaataat                                                                                    <  1:43427/150‑1 (MQ=255)
                                                                  ctTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTtaataata                                                                                   >  1:2142461/1‑150 (MQ=255)
                                                                   ttATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTtaataataa                                                                                  >  2:4010575/1‑150 (MQ=255)
                                                                       cATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATTAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGGAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGga                                                                              <  2:1769756/150‑1 (MQ=255)
                                                                       cATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGga                                                                              <  1:627748/150‑1 (MQ=255)
                                                                          aCTCACGGTTCAGATAGTAATGGAGATTATGTTGATTAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGGAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGaa                                                                           <  2:4699679/150‑1 (MQ=255)
                                                                           cTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAat                                                                          >  2:1722207/1‑150 (MQ=255)
                                                                              actgttcAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAATTAGCGATAAAAATCTTGTTAGAAGGAAAGATAATAATCTTGAATCATTTTATCTCTCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAatata                                                                       <  2:229422/144‑1 (MQ=255)
                                                                                       aTAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCa                                                              >  2:783803/1‑150 (MQ=255)
                                                                                       aTAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACAACTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCa                                                              >  2:783783/1‑150 (MQ=255)
                                                                                         aGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGa                                                            >  1:2326188/1‑150 (MQ=255)
                                                                                             aTGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGGAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATa                                                        >  1:2945936/1‑150 (MQ=255)
                                                                                              tgtcGATTATTTTTATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAg                                                       <  2:3421300/147‑1 (MQ=255)
                                                                                                gCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAgtg                                                     <  1:2127754/150‑1 (MQ=255)
                                                                                                gCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAgtg                                                     <  1:2128467/150‑1 (MQ=255)
                                                                                                   aTTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTcc                                                  >  1:601470/1‑150 (MQ=255)
                                                                                                    ttATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTccc                                                 <  1:1315803/150‑1 (MQ=255)
                                                                                                        gTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGa                                             <  2:1313113/150‑1 (MQ=255)
                                                                                                                 tttttttCAAGTAGCGATAAAAATCTTGTAAGAAGGAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCa                                    <  1:1465808/150‑1 (MQ=255)
                                                                                                                  ttttttCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAg                                   >  1:394596/1‑150 (MQ=255)
                                                                                                                    ttttCAAGTAGCGATAAAAATCTTGTAAGAAGGAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTa                                 <  1:488174/150‑1 (MQ=255)
                                                                                                                      ttCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGCTTGAGTTCAGTATg                               >  2:4061200/1‑150 (MQ=255)
                                                                                                                      ttCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATg                               >  2:1990809/1‑150 (MQ=255)
                                                                                                                      ttCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGAGTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATg                               >  2:1990908/1‑150 (MQ=255)
                                                                                                                          aGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATa                           >  2:4738058/1‑150 (MQ=255)
                                                                                                                           ttatataTAAAAATTTTGTTAGATTTAAATATAATTTTATTTAAGTATTTTATATAGCTACACATTATGGACGATTTTATGCTCTTAATTATAATGGAGAATATATTTTTATCAGAATTAGTGTGCCGGGATTGAGTTCAGTATGCATAc                          <  2:1976360/144‑1 (MQ=255)
                                                                                                                           gTATCGATAAAAATCTTGTAATAAGTAAAGATAATAATCTTTAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATAc                          <  2:1976363/150‑1 (MQ=255)
                                                                                                                           gTATCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATAc                          <  2:1975619/150‑1 (MQ=255)
                                                                                                                            tAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATAcc                         >  2:4201795/1‑150 (MQ=255)
                                                                                                                             aGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCg                        >  2:2153428/1‑150 (MQ=255)
                                                                                                                               cGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTa                      <  1:1796845/150‑1 (MQ=255)
                                                                                                                                gATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTAt                     <  2:4650902/150‑1 (MQ=255)
                                                                                                                                 aTAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATc                    <  2:207920/150‑1 (MQ=255)
                                                                                                                                  tAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCa                   >  2:1578549/1‑150 (MQ=255)
                                                                                                                                  tAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCa                   <  1:1829448/150‑1 (MQ=255)
                                                                                                                                    aaaaTCTTGTAAGAAGGAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATg                 <  2:3111589/150‑1 (MQ=255)
                                                                                                                                    aaaaTATTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATg                 <  2:3774952/150‑1 (MQ=255)
                                                                                                                                      aaTATTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGAt               <  2:1496985/150‑1 (MQ=255)
                                                                                                                                             tAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAAtttt        >  1:646523/1‑150 (MQ=255)
                                                                                                                                             tAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAAtttt        >  1:4679835/1‑150 (MQ=255)
                                                                                                                                              aagaagTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTa       <  1:777330/150‑1 (MQ=255)
                                                                                                                                              aagaagTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTa       >  2:4117347/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTAAGAAGTAAAGATAATAATCTTGAAGCATTTTATCTCGCAACACCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCT  >  CP009273/522711‑523007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A