Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 925,355 A→T 34.4% L355Q (CTG→CAG)  cydD ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273925,3550AT34.4% 348.7 / 313.7 273L355Q (CTG→CAG) cydDfused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base A (98/81);  new base T (36/58);  total (134/139)
Fisher's exact test for biased strand distribution p-value = 1.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 274 total aligned reads displayed.

CCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCG  >  CP009273/925208‑925504
                                                                                                                                                   |                                                                                                                                                     
cccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGc                                                                                                                                                     <  2:2320612/150‑1 (MQ=255)
cccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGc                                                                                                                                                     >  1:1645845/1‑150 (MQ=255)
   tGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGctcc                                                                                                                                                  >  1:3824602/1‑150 (MQ=255)
   tGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGctcc                                                                                                                                                  >  1:355369/1‑150 (MQ=255)
          agagaAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCt                                                                                                                                           <  2:4613405/150‑1 (MQ=255)
           gagaAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTc                                                                                                                                          >  2:1649711/1‑150 (MQ=255)
           gagaAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTc                                                                                                                                          <  2:4657613/150‑1 (MQ=255)
               aaaCCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCCCAATg                                                                                                                                      >  2:4368762/1‑150 (MQ=255)
                  ccAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTc                                                                                                                                   <  2:2022701/150‑1 (MQ=255)
                   cAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCa                                                                                                                                  >  1:1468177/1‑150 (MQ=255)
                    aGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCAc                                                                                                                                 <  1:3206234/150‑1 (MQ=255)
                    aGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCAc                                                                                                                                 <  1:3014386/150‑1 (MQ=255)
                     gAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCAcc                                                                                                                                <  2:1887597/150‑1 (MQ=255)
                      aaaGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCg                                                                                                                               >  1:4508709/1‑150 (MQ=255)
                       aaGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCgg                                                                                                                              >  2:3898829/1‑150 (MQ=255)
                       aaGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCgg                                                                                                                              >  2:2208447/1‑150 (MQ=255)
                         gcgcGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGAt                                                                                                                            <  2:704167/150‑1 (MQ=255)
                          cgcgTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATc                                                                                                                           <  1:723679/150‑1 (MQ=255)
                           gcgTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCg                                                                                                                          >  1:4234966/1‑150 (MQ=255)
                            cgTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCgg                                                                                                                         >  2:4156789/1‑150 (MQ=255)
                              ttCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTc                                                                                                                       <  1:3912792/150‑1 (MQ=255)
                               tCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCg                                                                                                                      >  1:1134656/1‑150 (MQ=255)
                               tCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCg                                                                                                                      <  2:1410801/150‑1 (MQ=255)
                                cagcagTGAGCTTTTACCTGAACCGCTGCGACCAACCCACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGa                                                                                                                     <  2:3365513/150‑1 (MQ=255)
                                       gAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAAt                                                                                                              >  1:2269486/1‑150 (MQ=255)
                                        aGCTTTGACCTGAACCGCTGCGACCAACCACCACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAAtt                                                                                                             <  2:2649455/150‑1 (MQ=255)
                                         gCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTc                                                                                                            <  1:1772651/150‑1 (MQ=255)
                                           ttttACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCg                                                                                                          <  1:2764049/150‑1 (MQ=255)
                                           ttttACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCg                                                                                                          <  1:635290/150‑1 (MQ=255)
                                              tACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCt                                                                                                       <  2:1373680/150‑1 (MQ=255)
                                              tACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCt                                                                                                       >  1:86153/1‑150 (MQ=255)
                                                 cTGAACCGCTGCGACCACCCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTcac                                                                                                    <  2:3042668/150‑1 (MQ=255)
                                                       cGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGtt                                                                                              <  1:3176694/150‑1 (MQ=255)
                                                        gCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTg                                                                                             <  1:328417/150‑1 (MQ=255)
                                                          tGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCg                                                                                           <  1:2599113/150‑1 (MQ=255)
                                                             gACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGAt                                                                                        <  1:3075536/150‑1 (MQ=255)
                                                              accaaccaACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATg                                                                                       <  1:4580968/150‑1 (MQ=255)
                                                                 aaccaacACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGc                                                                                    <  2:836005/150‑1 (MQ=255)
                                                                 aaccaacACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGc                                                                                    >  1:2514077/1‑150 (MQ=255)
                                                                   ccaacACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGa                                                                                  <  1:171764/150‑1 (MQ=255)
                                                                   ccaacACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGa                                                                                  <  1:1744118/150‑1 (MQ=255)
                                                                     aacACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGc                                                                                <  2:2269486/150‑1 (MQ=255)
                                                                     aacACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGc                                                                                >  2:4459532/1‑150 (MQ=255)
                                                                       cacCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCgg                                                                              <  1:4748798/150‑1 (MQ=255)
                                                                       cacCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCgg                                                                              >  1:1624438/1‑150 (MQ=255)
                                                                        acCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCggg                                                                             >  2:3918399/1‑150 (MQ=255)
                                                                         ccGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCgggg                                                                            >  1:2105209/1‑150 (MQ=255)
                                                                         ccGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCgggg                                                                            >  2:4700672/1‑150 (MQ=255)
                                                                         ccGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAAATCCGCCTCACCACGTGGCGGATGGGCGAGCgggg                                                                            >  2:2817664/1‑150 (MQ=255)
                                                                              cGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTcc                                                                       <  1:3318077/150‑1 (MQ=255)
                                                                                 tGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATa                                                                    >  2:4027490/1‑150 (MQ=255)
                                                                                  ggCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATaa                                                                   <  1:4659234/150‑1 (MQ=255)
                                                                                     ctgctgGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACg                                                                >  1:1791381/1‑150 (MQ=255)
                                                                                     ctgctgGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTGACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACg                                                                <  2:509118/150‑1 (MQ=255)
                                                                                       gctgGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGtt                                                              <  1:2069008/150‑1 (MQ=255)
                                                                                        ctgGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGttt                                                             >  2:3508752/1‑150 (MQ=255)
                                                                                         tgGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGtttt                                                            <  1:3685088/150‑1 (MQ=255)
                                                                                         tgGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGtttt                                                            <  1:1328496/150‑1 (MQ=255)
                                                                                            cAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAg                                                         >  2:2620811/1‑150 (MQ=255)
                                                                                             aaagtaaagtTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGa                                                        >  1:1007109/1‑150 (MQ=255)
                                                                                             aaagtaaagtTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGa                                                        >  2:1594520/1‑150 (MQ=255)
                                                                                              aagtaaagtTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGAc                                                       >  1:3464309/1‑150 (MQ=255)
                                                                                              aagtaaagtTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGAc                                                       >  1:839783/1‑150 (MQ=255)
                                                                                                gtaaagtTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTg                                                     <  1:103700/150‑1 (MQ=255)
                                                                                                  aaagtTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTc                                                   <  2:1163897/150‑1 (MQ=255)
                                                                                                   aagtTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCa                                                  >  1:2346406/1‑150 (MQ=255)
                                                                                                      ttCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCt                                               >  2:2387147/1‑150 (MQ=255)
                                                                                                            ggTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCa                                         >  1:1516376/1‑150 (MQ=255)
                                                                                                              tCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAAc                                       >  2:2525211/1‑150 (MQ=255)
                                                                                                              tCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAAc                                       >  1:3413500/1‑150 (MQ=255)
                                                                                                                   ccAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCt                                  >  2:1295586/1‑150 (MQ=255)
                                                                                                                   ccAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCt                                  <  1:3968429/150‑1 (MQ=255)
                                                                                                                   ccAGCGTTTTACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCt                                  >  2:3607995/1‑150 (MQ=255)
                                                                                                                   ccAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCt                                  >  1:1655011/1‑150 (MQ=255)
                                                                                                                     aGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTgg                                >  2:2078256/1‑150 (MQ=255)
                                                                                                                          tttACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGACttt                           <  2:4106824/150‑1 (MQ=255)
                                                                                                                           ttACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTa                          >  2:460534/1‑150 (MQ=255)
                                                                                                                           ttACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTa                          <  1:583645/150‑1 (MQ=255)
                                                                                                                           ttACCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTa                          <  1:2874178/150‑1 (MQ=255)
                                                                                                                           ttACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTa                          >  1:2401316/1‑150 (MQ=255)
                                                                                                                             aCCTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGc                        >  1:3803850/1‑150 (MQ=255)
                                                                                                                              ccTTCCGGCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCa                       >  2:4727577/1‑150 (MQ=255)
                                                                                                                               cTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCAt                      >  2:2671286/1‑150 (MQ=255)
                                                                                                                                 tCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGa                    <  2:3260984/150‑1 (MQ=255)
                                                                                                                                 tCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGa                    >  2:3756675/1‑150 (MQ=255)
                                                                                                                                  ccGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGAt                   >  1:944107/1‑150 (MQ=255)
                                                                                                                                     gCGACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATaaa                <  2:3321164/150‑1 (MQ=255)
                                                                                                                                     gCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATaaa                <  2:1774333/150‑1 (MQ=255)
                                                                                                                                       gACGTGATAAACTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGACAAAAc              <  1:4363442/150‑1 (MQ=255)
                                                                                                                                       gACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAAc              <  1:251530/150‑1 (MQ=255)
                                                                                                                                       gACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAAc              <  1:4176606/150‑1 (MQ=255)
                                                                                                                                         cGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGt            >  1:1293498/1‑150 (MQ=255)
                                                                                                                                          gTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTa           >  2:1733495/1‑150 (MQ=255)
                                                                                                                                            gATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTAcc         <  2:2056110/150‑1 (MQ=255)
                                                                                                                                             aTAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCg        >  1:1999585/1‑150 (MQ=255)
                                                                                                                                               aaaCTGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCgag      <  1:2971692/150‑1 (MQ=255)
                                                                                                                                               aaaCAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCgag      >  1:988704/1‑150 (MQ=255)
                                                                                                                                                 aCAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGAt    <  2:2864869/150‑1 (MQ=255)
                                                                                                                                                  cAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATc   <  2:1465768/150‑1 (MQ=255)
                                                                                                                                                   aGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATcg  <  1:4011707/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCG  >  CP009273/925208‑925504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A