Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,533,556 G→T 29.8% H1098N (CAC→AAC)  narZ ← nitrate reductase 2 (NRZ), alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,533,5560GT29.8% 660.6 / 291.0 348H1098N (CAC→AAC) narZnitrate reductase 2 (NRZ), alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (120/124);  new base T (61/43);  total (181/167)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

Only 100 of 350 total aligned reads displayed.

CGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGAT  >  CP009273/1533408‑1533705
                                                                                                                                                    |                                                                                                                                                     
  ggCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAAt                                                                                                                                                    <  2:1644767/150‑1 (MQ=255)
   gCAGTCAGCGCGCCGTTGGCGTTGAAGACGTCCACCCAGTCGTTCTCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATg                                                                                                                                                   <  2:666814/150‑1 (MQ=255)
      gTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGccc                                                                                                                                                >  2:3077294/1‑150 (MQ=255)
         aGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCAt                                                                                                                                             <  2:1189269/150‑1 (MQ=255)
           cgcgcCGTTGGCGTTGAATACTTCCACCCAGTCGTGATCGACAATGGTCAGGTCTCGGGCCTCTGTTTCGCGGATCCAGACAAACGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGGAGGTTGAGTGAATGCCCCAttt                                                                                                                                           >  2:2371598/1‑150 (MQ=255)
             cgcCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCt                                                                                                                                         <  1:2889883/150‑1 (MQ=255)
             cgcCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCt                                                                                                                                         <  1:31946/150‑1 (MQ=255)
              gcCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTg                                                                                                                                        <  2:1841177/150‑1 (MQ=255)
               ccGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTgg                                                                                                                                       <  2:3195064/150‑1 (MQ=255)
                 gTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTg                                                                                                                                     <  1:2914789/150‑1 (MQ=255)
                 gTTGGCGTTGAATACTTCCACCCAGTCGTTATAGACAAAGGTAAGTTATCGGGCATCTGTTTCGCTGATACACACAATCGGGCCACCGCGAGAGAGCGTCAGCATTAGCACGTTTTCACTTTAGGTTGAGTGAATTCCCCAATTCTgggt                                                                                                                                     >  1:2503108/1‑148 (MQ=255)
                  ttGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTGTCGGGCATCTGTTTCGCTGATCCAGACAAACGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTATGGTgc                                                                                                                                    >  1:4674915/1‑150 (MQ=255)
                  ttGGCGTTGAATACGTCCCCCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGCGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGGTGAGTGAATGCCCCATGTCTGGTgc                                                                                                                                    <  2:375939/150‑1 (MQ=255)
                         tGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCa                                                                                                                             <  1:2410691/150‑1 (MQ=255)
                           aaTACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAgg                                                                                                                           <  1:1568196/150‑1 (MQ=255)
                              aCTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAg                                                                                                                        <  1:1022128/150‑1 (MQ=255)
                                atCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGtt                                                                                                                      >  2:2692992/2‑150 (MQ=255)
                                      ccAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGc                                                                                                                >  1:339207/1‑150 (MQ=255)
                                        aGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCtt                                                                                                              <  2:1738696/150‑1 (MQ=255)
                                         gTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCttt                                                                                                             >  1:345736/1‑150 (MQ=255)
                                          tCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCtttt                                                                                                            <  1:3805897/150‑1 (MQ=255)
                                           cGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCttttt                                                                                                           <  2:3425732/150‑1 (MQ=255)
                                           cGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCttttt                                                                                                           <  1:1324847/150‑1 (MQ=255)
                                            gTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTc                                                                                                          >  2:366996/1‑150 (MQ=255)
                                            gTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTc                                                                                                          >  1:1909009/1‑150 (MQ=255)
                                            gTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTc                                                                                                          >  1:4117846/1‑150 (MQ=255)
                                             ttATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTcc                                                                                                         >  2:2411648/1‑150 (MQ=255)
                                              tATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCg                                                                                                        <  2:458170/150‑1 (MQ=255)
                                                tCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGGGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCggg                                                                                                      <  2:3526496/150‑1 (MQ=255)
                                                tCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCggg                                                                                                      <  2:3035740/150‑1 (MQ=255)
                                                 cGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGa                                                                                                     >  1:2603054/1‑150 (MQ=255)
                                                  gACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGaa                                                                                                    <  2:3469629/150‑1 (MQ=255)
                                                  gACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGaa                                                                                                    >  2:3322459/1‑150 (MQ=255)
                                                   aCAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAg                                                                                                   >  2:3268471/1‑150 (MQ=255)
                                                    cAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGc                                                                                                  >  1:1409483/1‑150 (MQ=255)
                                                      aTGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCg                                                                                                <  1:371861/150‑1 (MQ=255)
                                                       tGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGt                                                                                               >  1:4046200/1‑150 (MQ=255)
                                                         gTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGttt                                                                                             >  1:3476385/1‑150 (MQ=255)
                                                         gTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGttt                                                                                             >  1:2085308/1‑150 (MQ=255)
                                                            aGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGc                                                                                          <  2:2310591/150‑1 (MQ=255)
                                                             gTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCg                                                                                         <  1:16075/150‑1 (MQ=255)
                                                                ctcGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTa                                                                                      >  2:4133701/1‑150 (MQ=255)
                                                                   gggCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCt                                                                                   >  2:194414/1‑150 (MQ=255)
                                                                   gggCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCt                                                                                   <  2:4465375/150‑1 (MQ=255)
                                                                      cATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGgc                                                                                >  2:754311/1‑150 (MQ=255)
                                                                          tGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCAt                                                                            >  2:2800357/1‑150 (MQ=255)
                                                                          tGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCAt                                                                            <  1:3509999/150‑1 (MQ=255)
                                                                          tGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCAt                                                                            <  1:3283226/150‑1 (MQ=255)
                                                                            tttCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCAtct                                                                          <  1:3886208/150‑1 (MQ=255)
                                                                            tttCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCAtct                                                                          >  2:1382408/1‑150 (MQ=255)
                                                                             ttCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCAtctc                                                                         >  1:4382941/1‑150 (MQ=255)
                                                                                 cTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTg                                                                     >  1:2239542/1‑150 (MQ=255)
                                                                                  tGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGa                                                                    >  2:236852/1‑150 (MQ=255)
                                                                                  tGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGa                                                                    >  1:1102766/1‑150 (MQ=255)
                                                                                   gATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGAc                                                                   >  2:3877550/1‑150 (MQ=255)
                                                                                         gACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACg                                                             <  1:415277/150‑1 (MQ=255)
                                                                                            aaTCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGgt                                                          >  1:2939591/1‑150 (MQ=255)
                                                                                            aaTCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGgt                                                          <  2:338805/150‑1 (MQ=255)
                                                                                              tCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGgtgt                                                        >  2:1596233/1‑150 (MQ=255)
                                                                                               cGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTc                                                       <  2:4752610/150‑1 (MQ=255)
                                                                                                ggTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCg                                                      <  1:2341023/150‑1 (MQ=255)
                                                                                                ggTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCg                                                      <  2:3909212/150‑1 (MQ=255)
                                                                                                    cACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAg                                                  <  1:4744056/150‑1 (MQ=255)
                                                                                                    cACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAg                                                  <  1:4598154/150‑1 (MQ=255)
                                                                                                    cACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAg                                                  <  1:2194518/150‑1 (MQ=255)
                                                                                                         cgAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGGTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAggcggg                                             >  1:747156/1‑150 (MQ=255)
                                                                                                         cgAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAggcggg                                             <  1:2815234/150‑1 (MQ=255)
                                                                                                         cgAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAggcggg                                             <  2:4225012/150‑1 (MQ=255)
                                                                                                         cgAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAggcggg                                             <  2:126112/150‑1 (MQ=255)
                                                                                                          gagagaGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAggcgggc                                            >  2:818881/1‑150 (MQ=255)
                                                                                                                  gTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGcc                                    <  2:1690882/150‑1 (MQ=255)
                                                                                                                  gTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGcc                                    >  1:2047042/1‑150 (MQ=255)
                                                                                                                  gTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGcc                                    <  2:2174514/150‑1 (MQ=255)
                                                                                                                    cAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGccac                                  >  1:838184/1‑150 (MQ=255)
                                                                                                                     aGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGccacc                                 >  2:1068923/1‑150 (MQ=255)
                                                                                                                     aGCATTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGccacc                                 >  1:2273148/1‑150 (MQ=255)
                                                                                                                       cATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAg                               >  2:535086/1‑150 (MQ=255)
                                                                                                                        aTTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAgg                              >  1:4320539/1‑150 (MQ=255)
                                                                                                                        aTTAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAgg                              >  1:1918132/1‑150 (MQ=255)
                                                                                                                        aTTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAgg                              <  1:3965290/150‑1 (MQ=255)
                                                                                                                         ttAGCAGGTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGc                             >  2:40697/1‑150 (MQ=255)
                                                                                                                          tAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCt                            <  2:3914406/150‑1 (MQ=255)
                                                                                                                            gCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCttt                          <  2:2046628/150‑1 (MQ=255)
                                                                                                                              aGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCa                        <  1:3616086/150‑1 (MQ=255)
                                                                                                                               ggTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCAc                       >  2:471646/1‑150 (MQ=255)
                                                                                                                               ggTTTTCACTGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCAc                       >  2:1582194/1‑150 (MQ=255)
                                                                                                                               ggTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCAc                       >  1:1755971/1‑150 (MQ=255)
                                                                                                                                  tttCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCaa                    <  2:1416179/150‑1 (MQ=255)
                                                                                                                                  tttCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCaa                    <  2:4674915/150‑1 (MQ=255)
                                                                                                                                    tCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCaaaa                  >  2:959713/1‑150 (MQ=255)
                                                                                                                                     cACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAg                 >  1:1785951/1‑150 (MQ=255)
                                                                                                                                       cTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCa               >  2:3482312/1‑150 (MQ=255)
                                                                                                                                        tGTAGGTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCAc              <  1:148767/150‑1 (MQ=255)
                                                                                                                                          tAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGc            >  1:2226685/1‑150 (MQ=255)
                                                                                                                                           aGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCa           >  1:4474189/1‑150 (MQ=255)
                                                                                                                                           aGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGAGATAAGCCACCAGGCTTTCACCAAAAGCACGCa           >  1:1696/1‑150 (MQ=255)
                                                                                                                                            ggTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCAt          <  2:2267451/150‑1 (MQ=255)
                                                                                                                                            ggTTGAGTTAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCAt          >  2:2523093/1‑150 (MQ=255)
                                                                                                                                            ggTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCAt          <  1:4443216/150‑1 (MQ=255)
                                                                                                                                               tGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCa       >  2:2231420/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAGCAGGTTTTCACTGTAGGTTGAGTGAATGCCCCATTTCTGGTGCGGCGTCAGGAAGTTAAGTGCTTTTTCCGGGAAGCCGTTTGGCGGTATCTGGCGCATCTCACTGACGCTACGGGTGTCGATAGGCGGGCGATAAGCCACCAGGCTTTCACCAAAAGCACGCATCCACGGAT  >  CP009273/1533408‑1533705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A