Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,370,837 Δ1 bp 35.9% coding (235/759 nt) menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,370,8370C.35.9% 788.7 / inf 326coding (235/759 nt)menH2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand):  ref base C (98/110);  new base . (51/66);  total (149/177)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 328 total aligned reads displayed.

ATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATT  >  CP009273/2370688‑2370986
                                                                                                                                                     |                                                                                                                                                     
aTCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTc                                                                                                                                                       <  2:2832142/150‑1 (MQ=255)
 tCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCa                                                                                                                                                      >  1:874353/1‑150 (MQ=255)
    gAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCaatg                                                                                                                                                   >  1:3764485/1‑148 (MQ=255)
     aaCGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGa                                                                                                                                                  >  1:1296584/1‑150 (MQ=255)
     aaCGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGAt                                                                                                                                                 <  1:285956/150‑1 (MQ=255)
     aaCGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGAt                                                                                                                                                 <  1:444360/150‑1 (MQ=255)
          tGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGAtgttg                                                                                                                                             <  1:4426776/150‑1 (MQ=255)
          tGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGAtgttgt                                                                                                                                            <  2:3678102/150‑1 (MQ=255)
           gACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGta                                                                                                                                           <  2:1965510/150‑1 (MQ=255)
           gACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGta                                                                                                                                           <  2:2186739/150‑1 (MQ=255)
           gACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGta                                                                                                                                           >  2:2221732/1‑150 (MQ=255)
             cGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGtaac                                                                                                                                         <  2:2616080/150‑1 (MQ=255)
               ttCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGtaact                                                                                                                                        <  2:3898324/150‑1 (MQ=255)
                 ccGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGtaactaac                                                                                                                                     <  2:735018/150‑1 (MQ=255)
                  cGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGtaactaac                                                                                                                                     >  1:1889862/1‑150 (MQ=255)
                    cACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCa                                                                                                                                   >  2:3388100/1‑150 (MQ=255)
                     aCGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAg                                                                                                                                 <  2:2791302/150‑1 (MQ=255)
                      cGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAGGT‑AAGGATGTTGTAACTAACCAAgg                                                                                                                                <  2:114550/150‑1 (MQ=255)
                      cGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAg                                                                                                                                 <  1:2478034/150‑1 (MQ=255)
                      cGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAgg                                                                                                                                <  2:38390/150‑1 (MQ=255)
                       gttgttCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCaatg                                                                                                                                >  1:385443/1‑148 (MQ=255)
                       gttgttCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGt                                                                                                                               >  1:378225/1‑150 (MQ=255)
                        ttgttCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGtt                                                                                                                              <  2:2337892/150‑1 (MQ=255)
                          gttCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGtttt                                                                                                                            >  2:689044/1‑150 (MQ=255)
                          gttCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGtttt                                                                                                                            <  2:1310873/150‑1 (MQ=255)
                            tCAGCATTTTGCAGCCCCGGAGGCCCGCCTTCGACAATAACCCCACAACGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTa                                                                                                                           <  2:2727620/150‑1 (MQ=255)
                              aGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACg                                                                                                                         <  1:1561292/150‑1 (MQ=255)
                              aGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGc                                                                                                                        >  1:2920215/1‑150 (MQ=255)
                                 aTTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAg                                                                                                                      <  1:4772677/150‑1 (MQ=255)
                                   tttGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTa                                                                                                                    <  2:1265788/150‑1 (MQ=255)
                                       cAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCg                                                                                                               <  2:1868964/150‑1 (MQ=255)
                                       cAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCg                                                                                                               <  1:4032985/150‑1 (MQ=255)
                                        aGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCg                                                                                                               >  1:2087433/1‑150 (MQ=255)
                                            ccGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGa                                                                                                           <  1:3367872/150‑1 (MQ=255)
                                             cGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAc                                                                                                          >  1:1908904/1‑150 (MQ=255)
                                               gATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAcatc                                                                                                       <  1:2902164/150‑1 (MQ=255)
                                                aTGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAcatc                                                                                                       >  1:3239467/1‑150 (MQ=255)
                                                aTGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAcatc                                                                                                       >  1:1524951/1‑150 (MQ=255)
                                                aTGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAcatca                                                                                                      <  2:1347087/150‑1 (MQ=255)
                                                   cccGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACatcatc                                                                                                    <  2:3999417/150‑1 (MQ=255)
                                                   cccGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACAtcatca                                                                                                   <  1:767452/150‑1 (MQ=255)
                                                      gCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAAt                                                                                                >  2:1781434/1‑150 (MQ=255)
                                                       ccTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAAt                                                                                                <  1:935366/150‑1 (MQ=255)
                                                        cTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATc                                                                                               >  1:531405/1‑150 (MQ=255)
                                                        cTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATcc                                                                                              <  2:529210/150‑1 (MQ=255)
                                                         ttCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATcc                                                                                              >  1:570695/1‑150 (MQ=255)
                                                          tCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCa                                                                                             >  2:4595029/1‑150 (MQ=255)
                                                          tCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCAt                                                                                            <  1:1851000/150‑1 (MQ=255)
                                                           cGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCAt                                                                                            <  2:3048919/150‑1 (MQ=255)
                                                           cGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCAt                                                                                            >  1:965868/1‑150 (MQ=255)
                                                           cGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATc                                                                                           >  1:194090/1‑150 (MQ=255)
                                                             aCAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCg                                                                                          >  2:358785/1‑150 (MQ=255)
                                                                 tAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCt                                                                                     <  2:1171307/150‑1 (MQ=255)
                                                                    ccccACAAAGCCCCGCCAGCCAGTGGAAAGCCGCCATCATCGCCTCAGTTACACAAATCTAGTACCCCACCAGCCAGAAGTCAATTTTGTTGTAAATAAACAATGTTTTACGCTTTAAGTCTGTGAAATAATAAAATACATCGACGCTaa                                                                                   <  1:626957/150‑1 (MQ=255)
                                                                      ccACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCCTCGACGCTAATc                                                                                 <  2:570695/150‑1 (MQ=255)
                                                                      ccACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATc                                                                                 >  1:4064502/1‑150 (MQ=255)
                                                                         caAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATcgccg                                                                             <  1:709806/150‑1 (MQ=255)
                                                                           aaGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCCCGGATGGTGTACCTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCTGCAAATCCAGCGACGCTATTcgccgc                                                                            >  1:1675353/1‑150 (MQ=255)
                                                                              ccccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGa                                                                         <  1:3174597/150‑1 (MQ=255)
                                                                                ccGCCAGCCCCGGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAac                                                                       <  1:4442630/150‑1 (MQ=255)
                                                                                  gCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAacca                                                                     <  1:4137315/150‑1 (MQ=255)
                                                                                  gCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAaccac                                                                    >  2:421403/1‑150 (MQ=255)
                                                                                  gCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAaccac                                                                    >  1:4367399/1‑150 (MQ=255)
                                                                                    cAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAaccacc                                                                   >  1:2228414/1‑150 (MQ=255)
                                                                                     aGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCg                                                                  >  1:2565577/1‑150 (MQ=255)
                                                                                       ccccTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGa                                                               <  2:4380735/150‑1 (MQ=255)
                                                                                         ccTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGAcc                                                             <  2:3781399/150‑1 (MQ=255)
                                                                                         ccTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGAcc                                                             <  2:1446962/150‑1 (MQ=255)
                                                                                           tGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCt                                                            >  2:2573480/1‑150 (MQ=255)
                                                                                            ggCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTgg                                                          <  2:2745668/150‑1 (MQ=255)
                                                                                              cAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTggg                                                         <  2:4437608/150‑1 (MQ=255)
                                                                                               aaGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGga                                                        >  1:4619374/1‑150 (MQ=255)
                                                                                               aaGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGgag                                                       >  2:4303932/1‑150 (MQ=255)
                                                                                                      catcatCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAAc                                                 <  1:1968181/150‑1 (MQ=255)
                                                                                                      caacatcGCCACCAGTCCTCCATGCGTGTACCCCACCAGACAGATGTCTAGGTTTTTGTAACTAACCAAGGTTTTACGCAGTAAGTGGGTGACATAATCAATTCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAAc                                                 <  1:2902906/147‑1 (MQ=255)
                                                                                                       atcatcGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGCGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACa                                                >  1:3059748/1‑150 (MQ=255)
                                                                                                       atcatcGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACAt                                               <  1:3698601/150‑1 (MQ=255)
                                                                                                       atcatcGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACAt                                               >  2:3297358/1‑150 (MQ=255)
                                                                                                           tcGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACa                                            >  2:1849841/1‑150 (MQ=255)
                                                                                                           tcGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACa                                            <  1:1233619/150‑1 (MQ=255)
                                                                                                              ccACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAAcc                                         <  1:1006264/150‑1 (MQ=255)
                                                                                                               cACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCt                                                            >  1:3903529/1‑129 (MQ=255)
                                                                                                                   cGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGag                                    <  2:506034/150‑1 (MQ=255)
                                                                                                                    gTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGagt                                   >  2:586353/1‑150 (MQ=255)
                                                                                                                       caccaAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGagtagt                                <  1:2764843/150‑1 (MQ=255)
                                                                                                                         ccaAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCg                              <  2:2963898/150‑1 (MQ=255)
                                                                                                                            cgCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCa                           <  2:1737842/149‑1 (MQ=255)
                                                                                                                            aGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCa                           <  2:905862/150‑1 (MQ=255)
                                                                                                                             gCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCaa                          <  1:1138473/150‑1 (MQ=255)
                                                                                                                              cGAGTACCCCACCAGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAAc                        <  2:4102226/150‑1 (MQ=255)
                                                                                                                               gAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAAc                        >  1:2375024/1‑150 (MQ=255)
                                                                                                                               gAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGGGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAAc                        >  1:2132180/1‑150 (MQ=255)
                                                                                                                                    ccccACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGAAGTAAGTCGGTTACATCATAAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTc                   <  1:224582/150‑1 (MQ=255)
                                                                                                                                     cccACCAGCAAGACGTCAAGGATTTTGTAAATAACAATGTTTTTACGCATTATGTCGGTTAAATCAAAAAATCCATCGACGCTAATCGCCGCCGAACCCCCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCg                  <  1:3348334/150‑1 (MQ=255)
                                                                                                                                      ccaccaGCCAGAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGcc                >  1:3575802/1‑150 (MQ=255)
                                                                                                                                              cagAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCActtct         >  1:4009246/1‑150 (MQ=255)
                                                                                                                                              cagAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCActtct         >  1:3665943/1‑150 (MQ=255)
                                                                                                                                              cagAAGT‑AAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCActtctt        >  1:1724504/1‑150 (MQ=255)
                                                                                                                                                gAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTg       >  1:2775181/1‑150 (MQ=255)
                                                                                                                                                     cAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCAtt  >  2:1498893/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATT  >  CP009273/2370688‑2370986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A