Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 306,510 G→A 22.7% R180W (CGG→TGG)  ecpR ← putative transcriptional regulator for the ecp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273306,5100GA22.7% 478.6 / 139.7 220R180W (CGG→TGG) ecpRputative transcriptional regulator for the ecp operon
Reads supporting (aligned to +/- strand):  ref base G (84/86);  new base A (32/18);  total (116/104)
Fisher's exact test for biased strand distribution p-value = 7.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

GCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCA  >  CP009273/306363‑306659
                                                                                                                                                   |                                                                                                                                                     
gCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGAt                                                                                                                                                     <  2:435723/150‑1 (MQ=255)
gCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGAt                                                                                                                                                     <  1:2773466/150‑1 (MQ=255)
        cTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATac                                                                                                                                             <  1:374862/150‑1 (MQ=255)
         ttttttttCATTGCATTTCGTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATaca                                                                                                                                            <  2:4693917/150‑1 (MQ=255)
          tttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATacac                                                                                                                                           <  2:119733/150‑1 (MQ=255)
           ttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACt                                                                                                                                          <  1:689632/150‑1 (MQ=255)
           ttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACt                                                                                                                                          >  1:442214/1‑150 (MQ=255)
           ttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACt                                                                                                                                          <  1:437493/150‑1 (MQ=255)
             ttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGt                                                                                                                                        <  1:1090106/150‑1 (MQ=255)
             ttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGAGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGACTGGAGTTTACTGAACAAACTTATATATTTTTGAGTACAGCTTGGCCTCAGAATTACTCCAATGGGTATACACTGt                                                                                                                                        >  2:1438194/1‑150 (MQ=255)
                 cATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTc                                                                                                                                    >  2:3060982/1‑150 (MQ=255)
                 cATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTc                                                                                                                                    >  2:1961446/1‑150 (MQ=255)
                      cATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACt                                                                                                                               >  1:1931015/1‑150 (MQ=255)
                      cATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACt                                                                                                                               >  1:1201542/1‑150 (MQ=255)
                      cATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACt                                                                                                                               <  2:361965/150‑1 (MQ=255)
                        tttCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTAc                                                                                                                             >  2:181322/1‑150 (MQ=255)
                         ttcttcCCGAGTTGAATTGAGGAGGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACa                                                                                                                            <  1:2540385/150‑1 (MQ=255)
                         ttcttcCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACa                                                                                                                            <  1:2444853/150‑1 (MQ=255)
                           cttcCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATAAACTGTCTTCACACTACAAt                                                                                                                          >  1:4856457/1‑150 (MQ=255)
                               ccGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTc                                                                                                                      <  2:4619888/150‑1 (MQ=255)
                                 gAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCaa                                                                                                                    >  1:3914499/1‑150 (MQ=255)
                                  aGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAAATTATATATTTTTGAGTACAGCTTGGCATCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAAt                                                                                                                   >  2:4234576/1‑150 (MQ=255)
                                   gTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAAtt                                                                                                                  >  2:1393311/1‑150 (MQ=255)
                                       aaTTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTgg                                                                                                              >  2:1124669/1‑150 (MQ=255)
                                       aaTTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTgg                                                                                                              <  1:3767276/150‑1 (MQ=255)
                                        aTTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGc                                                                                                             <  1:2100204/150‑1 (MQ=255)
                                          tGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCaa                                                                                                           >  2:3993546/1‑150 (MQ=255)
                                           gAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCact                                                                                                          >  1:2689530/1‑148 (MQ=255)
                                           gAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAAt                                                                                                          <  1:2298526/150‑1 (MQ=255)
                                            aGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGATTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGTCACTc                                                                                                         >  2:263080/1‑150 (MQ=255)
                                               aCGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGAt                                                                                                      <  1:4557184/150‑1 (MQ=255)
                                                cGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGAtt                                                                                                     >  1:1894704/1‑150 (MQ=255)
                                                 gTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGAttt                                                                                                    <  2:851050/150‑1 (MQ=255)
                                                 gTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGAttt                                                                                                    <  1:3602126/150‑1 (MQ=255)
                                                  tGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTa                                                                                                   <  1:498824/150‑1 (MQ=255)
                                                    aTGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAgg                                                                                                 <  2:1851890/150‑1 (MQ=255)
                                                    aTGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAgg                                                                                                 <  1:1122942/150‑1 (MQ=255)
                                                      gTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGtt                                                                                               >  1:1691315/1‑150 (MQ=255)
                                                       tCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTg                                                                                              <  1:4312141/150‑1 (MQ=255)
                                                       tCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTg                                                                                              >  2:3762852/1‑150 (MQ=255)
                                                          cAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCAt                                                                                           >  1:245632/1‑150 (MQ=255)
                                                          cAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCAt                                                                                           <  2:2338076/150‑1 (MQ=255)
                                                            gTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTc                                                                                         >  1:3289273/1‑150 (MQ=255)
                                                            gTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTc                                                                                         <  2:2943976/150‑1 (MQ=255)
                                                                  cATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTggg                                                                                   <  1:607416/150‑1 (MQ=255)
                                                                  cATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTggg                                                                                   <  1:3037857/150‑1 (MQ=255)
                                                                          aaaaCTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATg                                                                           >  1:823058/1‑150 (MQ=255)
                                                                           aaaCTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGc                                                                          <  2:2999881/150‑1 (MQ=255)
                                                                           aaaCTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGc                                                                          >  1:1511566/1‑150 (MQ=255)
                                                                               taactTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGAt                                                                      <  1:336207/150‑1 (MQ=255)
                                                                               taactTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGAt                                                                      <  1:2913615/150‑1 (MQ=255)
                                                                                aactTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATa                                                                     >  2:892634/1‑150 (MQ=255)
                                                                                 actTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATaa                                                                    <  2:3337594/150‑1 (MQ=255)
                                                                                 acgtGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATaa                                                                    <  2:1993820/147‑1 (MQ=255)
                                                                                   ttGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAatt                                                                  <  1:1100200/150‑1 (MQ=255)
                                                                                    tGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAattt                                                                 <  1:3502933/150‑1 (MQ=255)
                                                                                    tGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAattt                                                                 <  1:1047393/150‑1 (MQ=255)
                                                                                        tGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAatttccat                                                             >  2:1314238/1‑150 (MQ=255)
                                                                                        tGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAatttccat                                                             >  1:1700782/1‑150 (MQ=255)
                                                                                        gggAGATTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTAAATTCTGGAAATCGATTTAGGTTGCATTCCCTGGGCAGTCATGCGGATAatttccat                                                             >  2:2762528/2‑150 (MQ=255)
                                                                                          gAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAatttccattt                                                           <  1:790655/150‑1 (MQ=255)
                                                                                              ttACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCg                                                       <  2:1738469/150‑1 (MQ=255)
                                                                                              ttACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCg                                                       >  1:2535987/1‑150 (MQ=255)
                                                                                              ttACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCg                                                       >  2:2276238/1‑150 (MQ=255)
                                                                                              ttACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCg                                                       >  2:2277518/1‑150 (MQ=255)
                                                                                               tACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCgg                                                      <  1:3162490/150‑1 (MQ=255)
                                                                                                 cTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTc                                                    >  1:3653260/1‑150 (MQ=255)
                                                                                                   gAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCg                                                  >  2:863962/1‑150 (MQ=255)
                                                                                                    aaCCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGt                                                 >  1:921887/1‑150 (MQ=255)
                                                                                                    aaCCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGt                                                 >  1:541466/1‑150 (MQ=255)
                                                                                                          cTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGAtttt                                           >  1:1830507/1‑150 (MQ=255)
                                                                                                          cTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGAtttt                                           <  1:1419369/150‑1 (MQ=255)
                                                                                                             atatatTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGAATTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTAtc                                        >  1:4419642/1‑150 (MQ=255)
                                                                                                             atatatTTTTGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTAtc                                        <  1:2683537/150‑1 (MQ=255)
                                                                                                              tatatTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTAtct                                       <  2:442214/150‑1 (MQ=255)
                                                                                                                 atTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTAtctttc                                    >  1:1538247/1‑150 (MQ=255)
                                                                                                                  tttttGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTAtctttct                                   <  2:4814836/150‑1 (MQ=255)
                                                                                                                  tttttGAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTAtctttct                                   <  2:3299218/150‑1 (MQ=255)
                                                                                                                     ttGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTaa                                >  2:526842/1‑150 (MQ=255)
                                                                                                                       gAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAata                              >  1:983059/1‑150 (MQ=255)
                                                                                                                       gAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAata                              >  2:1542714/1‑150 (MQ=255)
                                                                                                                       gAGTACAGCTTGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAata                              <  1:3685628/150‑1 (MQ=255)
                                                                                                                         gTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATc                            >  2:4157169/1‑150 (MQ=255)
                                                                                                                           aCAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCtt                          >  1:4130429/1‑150 (MQ=255)
                                                                                                                            cAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCttt                         <  1:2585878/150‑1 (MQ=255)
                                                                                                                                ttGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTtctc                     >  1:2327516/1‑150 (MQ=255)
                                                                                                                                 tGGCCTCAGCATTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATATTTTCTCa                    >  2:2447731/1‑150 (MQ=255)
                                                                                                                                  ggCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAg                   >  2:4507105/1‑150 (MQ=255)
                                                                                                                                  ggCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAg                   <  2:2123510/150‑1 (MQ=255)
                                                                                                                                    ccTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGa                 <  1:2894591/150‑1 (MQ=255)
                                                                                                                                        aGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCt             >  1:1997455/1‑150 (MQ=255)
                                                                                                                                           aTTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGcc          >  1:1491927/1‑150 (MQ=255)
                                                                                                                                           aTTACGCCAATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGcc          >  2:3146266/1‑150 (MQ=255)
                                                                                                                                             tACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCAt        >  1:3865804/1‑150 (MQ=255)
                                                                                                                                              aCGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCAtt       >  2:342293/1‑150 (MQ=255)
                                                                                                                                              aCGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCAtt       >  2:342647/1‑150 (MQ=255)
                                                                                                                                               cGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTa      >  2:4419904/1‑150 (MQ=255)
                                                                                                                                                  cGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATc   >  2:2986730/1‑150 (MQ=255)
                                                                                                                                                  cGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATc   >  2:556582/1‑150 (MQ=255)
                                                                                                                                                   gATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATca  <  2:4383777/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCA  >  CP009273/306363‑306659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A