Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 333,621 G→T 21.3% R378L (CGC→CTC)  yahF → putative NAD(P)‑binding succinyl‑CoA synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273333,6210GT21.3% 737.5 / 160.0 276R378L (CGC→CTC) yahFputative NAD(P)‑binding succinyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base G (95/122);  new base T (35/24);  total (130/146)
Fisher's exact test for biased strand distribution p-value = 3.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

TTGCGGATGTGCGTGCGCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTG  >  CP009273/333474‑333769
                                                                                                                                                   |                                                                                                                                                    
 tGCGGATGTGCGTGCGCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTcctcct                                                                                                                                                   >  1:3939311/1‑150 (MQ=255)
  gCGGATGTGCGTGCGCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTg                                                                                                                                                  <  1:3560635/150‑1 (MQ=255)
   cGGATGTGCGTGCGCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGa                                                                                                                                                 <  2:15512/150‑1 (MQ=255)
    ggATGTGCGTGCGCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGaa                                                                                                                                                >  2:4741660/1‑150 (MQ=255)
    ggATGTGCGTGCGCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGaa                                                                                                                                                >  2:2791560/1‑150 (MQ=255)
     gATGTGCGTGCGCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGaaa                                                                                                                                               >  2:1746230/1‑150 (MQ=255)
      aTGTGCGTGCGCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAg                                                                                                                                              <  2:1534484/150‑1 (MQ=255)
         tgcgtgcgCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGata                                                                                                                                           >  2:1202471/1‑150 (MQ=255)
         tgcgtgcgCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGata                                                                                                                                           <  2:1610915/150‑1 (MQ=255)
           cgtgcgCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATc                                                                                                                                         <  2:2400407/150‑1 (MQ=255)
           cgtgcgCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATc                                                                                                                                         >  1:2494395/1‑150 (MQ=255)
            gtgcgCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAATCCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCa                                                                                                                                        <  2:1010462/150‑1 (MQ=255)
            gtgcgCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCa                                                                                                                                        <  2:2341655/150‑1 (MQ=255)
             tgcgCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCaa                                                                                                                                       <  1:4461893/150‑1 (MQ=255)
                gcgTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCg                                                                                                                                    <  1:4270330/150‑1 (MQ=255)
                gcgTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCg                                                                                                                                    >  1:1391318/1‑150 (MQ=255)
                     tGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCgcagca                                                                                                                               >  2:4197866/1‑150 (MQ=255)
                        aaCCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCa                                                                                                                            >  1:234385/1‑150 (MQ=255)
                        aaCCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCa                                                                                                                            >  2:4400419/1‑150 (MQ=255)
                           cGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAac                                                                                                                         <  1:3110895/150‑1 (MQ=255)
                             cagcagAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAacac                                                                                                                       >  1:4110358/1‑150 (MQ=255)
                             cagcagAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAacac                                                                                                                       >  1:3321886/1‑150 (MQ=255)
                              agcagAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAacaca                                                                                                                      <  1:950584/150‑1 (MQ=255)
                                cagAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACAcc                                                                                                                    >  1:1647578/1‑150 (MQ=255)
                                  gAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCtt                                                                                                                  >  2:1660615/1‑150 (MQ=255)
                                     aTACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCt                                                                                                               <  1:1982534/150‑1 (MQ=255)
                                       aCATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCg                                                                                                             <  2:1046042/150‑1 (MQ=255)
                                       aCATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCg                                                                                                             >  2:3093623/1‑150 (MQ=255)
                                              tGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTgg                                                                                                      >  1:3726815/1‑150 (MQ=255)
                                                 ccTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGa                                                                                                   >  2:2625184/1‑150 (MQ=255)
                                                   tGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATg                                                                                                 <  1:1253916/150‑1 (MQ=255)
                                                   tGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATg                                                                                                 <  1:2579288/150‑1 (MQ=255)
                                                   tGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATg                                                                                                 >  1:4172998/1‑150 (MQ=255)
                                                     ttCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATgac                                                                                               <  2:4132151/150‑1 (MQ=255)
                                                      tCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATgacg                                                                                              <  1:2135027/150‑1 (MQ=255)
                                                          cggcggCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACtt                                                                                          <  2:1740402/150‑1 (MQ=255)
                                                           ggcggcACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTc                                                                                         >  2:2961381/1‑150 (MQ=255)
                                                            gcggcACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCa                                                                                        <  1:1704014/150‑1 (MQ=255)
                                                              ggcACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCAcc                                                                                      <  2:1863640/150‑1 (MQ=255)
                                                               gcACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCa                                                                                     >  2:542694/1‑150 (MQ=255)
                                                               gcACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCa                                                                                     <  1:1511469/150‑1 (MQ=255)
                                                                cACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTGTGGCGATGACGACTTCACCaa                                                                                    >  2:3334590/1‑150 (MQ=255)
                                                                  cGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATg                                                                                  >  1:430286/1‑150 (MQ=255)
                                                                  cGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATg                                                                                  <  2:4805800/150‑1 (MQ=255)
                                                                   gCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATgg                                                                                 >  2:2899504/1‑150 (MQ=255)
                                                                   gCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATgg                                                                                 <  2:22927/150‑1 (MQ=255)
                                                                    cTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGc                                                                                >  1:1334960/1‑150 (MQ=255)
                                                                      gtgCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCaa                                                                              <  1:3762318/150‑1 (MQ=255)
                                                                      gtgCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCaa                                                                              >  2:3666069/1‑150 (MQ=255)
                                                                          gacgaAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCAACAATGGCAAGCCg                                                                          <  2:354979/150‑1 (MQ=255)
                                                                              aaaCCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCAcc                                                                      >  1:4579214/1‑150 (MQ=255)
                                                                              aaaCCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCAcc                                                                      >  1:1038019/1‑150 (MQ=255)
                                                                              aaaCCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCAcc                                                                      >  1:4317222/1‑150 (MQ=255)
                                                                                  cATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCgat                                                                  <  1:3267643/150‑1 (MQ=255)
                                                                                  cATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCgat                                                                  <  1:3397355/150‑1 (MQ=255)
                                                                                      ttCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGAtt                                                              <  1:665040/150‑1 (MQ=255)
                                                                                             tGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCcca                                                       >  2:3602034/1‑150 (MQ=255)
                                                                                               aTGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCccacc                                                     >  2:2198501/1‑150 (MQ=255)
                                                                                                 ggAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCaa                                                   >  1:2767899/1‑150 (MQ=255)
                                                                                                 ggAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCaa                                                   <  1:4741974/150‑1 (MQ=255)
                                                                                                  gAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAAc                                                  <  1:2720012/150‑1 (MQ=255)
                                                                                                   aaaaaCATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAAcc                                                 >  2:4097434/1‑150 (MQ=255)
                                                                                                     aaaCATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGc                                               >  1:1946481/1‑150 (MQ=255)
                                                                                                       aCATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCAt                                             <  1:4066685/150‑1 (MQ=255)
                                                                                                        cATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATc                                            <  1:2950845/150‑1 (MQ=255)
                                                                                                         aTGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCa                                           <  1:1565516/150‑1 (MQ=255)
                                                                                                          tGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAg                                          >  2:3674439/1‑150 (MQ=255)
                                                                                                          tGGCGATGTCTACAGCAACATTCAGCCAGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAg                                          <  1:1338861/150‑1 (MQ=255)
                                                                                                           ggCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGt                                         <  1:4688050/150‑1 (MQ=255)
                                                                                                             cGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCg                                       <  1:4795571/150‑1 (MQ=255)
                                                                                                             cGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCg                                       >  1:3216136/1‑150 (MQ=255)
                                                                                                               aTGTCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCt                                     >  2:3791807/1‑150 (MQ=255)
                                                                                                                tGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCtt                                    >  1:843099/1‑150 (MQ=255)
                                                                                                                  tCTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGa                                  <  1:796006/150‑1 (MQ=255)
                                                                                                                  tCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGa                                  <  1:833258/150‑1 (MQ=255)
                                                                                                                  tCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGa                                  <  1:1638320/150‑1 (MQ=255)
                                                                                                                  tCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGa                                  >  2:2571321/1‑150 (MQ=255)
                                                                                                                   cTACAGCAACATTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGAt                                 <  2:3321788/150‑1 (MQ=255)
                                                                                                                   cTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGAt                                 >  2:4535409/1‑150 (MQ=255)
                                                                                                                   cTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGAt                                 >  1:1147427/1‑150 (MQ=255)
                                                                                                                    tACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATc                                <  1:4875153/150‑1 (MQ=255)
                                                                                                                    tACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATc                                >  2:1043099/1‑150 (MQ=255)
                                                                                                                            cATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGgc                        >  1:3434618/1‑150 (MQ=255)
                                                                                                                             aTTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGgcg                       <  2:697630/150‑1 (MQ=255)
                                                                                                                             aTTCAGCCCGATCCGGAATTCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGgcg                       >  1:307652/1‑150 (MQ=255)
                                                                                                                               tCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGgcgcg                     >  2:843346/1‑150 (MQ=255)
                                                                                                                               tCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGgcgcg                     >  2:3749890/1‑150 (MQ=255)
                                                                                                                                 aGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGcgcgcg                   <  2:1408809/150‑1 (MQ=255)
                                                                                                                                     cGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATcc               >  2:1897938/1‑150 (MQ=255)
                                                                                                                                      gATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCa              >  1:1814502/1‑150 (MQ=255)
                                                                                                                                       aTCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAg             >  2:3619644/1‑150 (MQ=255)
                                                                                                                                       aTCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAg             >  2:2465136/1‑150 (MQ=255)
                                                                                                                                        tCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGa            >  1:2879131/1‑150 (MQ=255)
                                                                                                                                           ggAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGt         >  2:3932935/1‑150 (MQ=255)
                                                                                                                                            gAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTg        <  2:234385/150‑1 (MQ=255)
                                                                                                                                             aaTTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTgg       <  2:4489954/150‑1 (MQ=255)
                                                                                                                                             aaTTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATAAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTgg       <  1:3093623/150‑1 (MQ=255)
                                                                                                                                               ttCCTCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCg     >  1:2461875/1‑150 (MQ=255)
                                                                                                                                                 ccGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGt   <  2:1233941/150‑1 (MQ=255)
                                                                                                                                                  cGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTg  <  1:1189356/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTGCGGATGTGCGTGCGCGTCTGCAACCGCAGCAGAAATACATTCGTGGCCTGTTCTGCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTG  >  CP009273/333474‑333769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A