Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,087,742 A→T 21.5% Y2N (TAT→AAT)  pgaA ← biofilm adhesin polysaccharide PGA secretin; OM porin; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,087,7420AT21.5% 391.8 / 101.0 158Y2N (TAT→AAT) pgaAbiofilm adhesin polysaccharide PGA secretin; OM porin; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein
Reads supporting (aligned to +/- strand):  ref base A (49/75);  new base T (16/18);  total (65/93)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

AGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGAAGAAGAAATGATGA  >  CP009273/1087596‑1087890
                                                                                                                                                  |                                                                                                                                                    
tgTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACAt                                                                                                                                                   >  2:3488857/2‑150 (MQ=255)
tgTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACAt                                                                                                                                                   >  2:1033234/2‑150 (MQ=255)
   aTTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCt                                                                                                                                                >  1:2012656/1‑150 (MQ=255)
   aTTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCt                                                                                                                                                <  1:4345640/150‑1 (MQ=255)
    ttACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTg                                                                                                                                               >  1:1434957/1‑150 (MQ=255)
     tACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGt                                                                                                                                              >  1:2786800/1‑150 (MQ=255)
     tACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGt                                                                                                                                              <  1:4317515/150‑1 (MQ=255)
      aCCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCGTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTa                                                                                                                                             <  2:4008158/150‑1 (MQ=255)
      aCCCTTGCGAGCTTCAAGACTCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTa                                                                                                                                             <  2:2966697/150‑1 (MQ=255)
             cGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTcc                                                                                                                                      <  1:849332/150‑1 (MQ=255)
              gAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCa                                                                                                                                     <  1:1237674/150‑1 (MQ=255)
               aGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCAt                                                                                                                                    <  1:1774849/150‑1 (MQ=255)
               aGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCAt                                                                                                                                    <  1:1774152/150‑1 (MQ=255)
                gCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATg                                                                                                                                   >  1:2224284/1‑150 (MQ=255)
                  ttCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTa                                                                                                                                 >  2:1153924/1‑150 (MQ=255)
                   tCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTAt                                                                                                                                <  2:3360836/150‑1 (MQ=255)
                           cAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCaaaa                                                                                                                        >  2:3641857/1‑150 (MQ=255)
                            aaTGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAAt                                                                                                                       >  2:3925737/1‑150 (MQ=255)
                            aaTGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAAt                                                                                                                       <  2:2260636/150‑1 (MQ=255)
                                catcatAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAAtctct                                                                                                                   >  1:4620195/1‑150 (MQ=255)
                                 atcatAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCAGTATTACTCCATGTATTTCCAAAAtctctc                                                                                                                  >  2:3331724/1‑150 (MQ=255)
                                 atcatAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAAtctctc                                                                                                                  <  2:2731418/150‑1 (MQ=255)
                                  tcatAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                                 <  1:2998190/150‑1 (MQ=255)
                                  tcatAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                                 <  1:4172157/150‑1 (MQ=255)
                                  tcatAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                                 <  1:4172150/150‑1 (MQ=255)
                                       ggCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCACAGCCCATTTGGTTTTCGGGCACCTTTTTCTTCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTa                                                                                                            <  1:3292459/150‑1 (MQ=255)
                                        gCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt                                                                                                           <  2:1576211/150‑1 (MQ=255)
                                           ttattaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTa                                                                                                        >  1:3103827/1‑150 (MQ=255)
                                            tattaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTaa                                                                                                       >  2:1940045/1‑150 (MQ=255)
                                              ttaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAAtt                                                                                                     <  1:2713005/150‑1 (MQ=255)
                                              ttaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAAtt                                                                                                     <  2:1627750/150‑1 (MQ=255)
                                              ttaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAAtt                                                                                                     >  1:2974713/1‑150 (MQ=255)
                                               taACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTa                                                                                                    <  1:2493760/150‑1 (MQ=255)
                                                   aGCACTCGTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAgg                                                                                                <  1:4712475/150‑1 (MQ=255)
                                                    gCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGt                                                                                               <  1:4462535/150‑1 (MQ=255)
                                                     cACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTa                                                                                              <  2:3800594/150‑1 (MQ=255)
                                                     cACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTa                                                                                              <  2:1212565/150‑1 (MQ=255)
                                                      aCTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTaa                                                                                             <  2:4459876/150‑1 (MQ=255)
                                                      aCTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTaa                                                                                             >  2:1656003/1‑150 (MQ=255)
                                                      aCTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTaa                                                                                             >  1:600451/1‑150 (MQ=255)
                                                         ctTCGCCGCGGGACTAGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTg                                                                                          >  2:2673537/1‑150 (MQ=255)
                                                          ttCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGa                                                                                         >  2:90326/1‑150 (MQ=255)
                                                          ttCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGa                                                                                         >  2:3515291/1‑150 (MQ=255)
                                                           tCGCCGTGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGaa                                                                                        <  1:1509532/150‑1 (MQ=255)
                                                           tCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGaa                                                                                        <  2:1095324/150‑1 (MQ=255)
                                                            cgccgcGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGaaa                                                                                       <  2:3160642/150‑1 (MQ=255)
                                                            cgccgcGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGaaa                                                                                       >  1:4412782/1‑150 (MQ=255)
                                                               cgcgGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAaaaga                                                                                    >  1:1653669/1‑150 (MQ=255)
                                                                 cgGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAaaagaaa                                                                                  <  1:939989/150‑1 (MQ=255)
                                                                  gggACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAaaagaaag                                                                                 <  2:4067600/150‑1 (MQ=255)
                                                                   ggACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGTAaagaaaga                                                                                >  2:4187557/1‑150 (MQ=255)
                                                                    gACTCTCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGat                                                                               <  2:2625506/150‑1 (MQ=255)
                                                                    gACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGat                                                                               <  2:195901/150‑1 (MQ=255)
                                                                     aCTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGata                                                                              <  2:2851688/150‑1 (MQ=255)
                                                                      cTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGatat                                                                             >  1:1338057/1‑150 (MQ=255)
                                                                       tCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATAtt                                                                            <  1:2757777/150‑1 (MQ=255)
                                                                        cGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATAttt                                                                           <  2:668425/150‑1 (MQ=255)
                                                                         gcttatAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTATTACTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATAtttt                                                                          <  2:4027801/145‑1 (MQ=255)
                                                                         gctgctAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATAtttt                                                                          <  1:3131903/150‑1 (MQ=255)
                                                                           tgctAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTg                                                                        >  1:1992013/1‑150 (MQ=255)
                                                                            gctAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGc                                                                       <  1:412637/150‑1 (MQ=255)
                                                                            gctAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGc                                                                       <  1:967539/150‑1 (MQ=255)
                                                                             ctAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCa                                                                      <  2:4291880/150‑1 (MQ=255)
                                                                             ctAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCa                                                                      <  2:1838864/150‑1 (MQ=255)
                                                                             ctAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCa                                                                      <  2:1139579/150‑1 (MQ=255)
                                                                               aaaaaTGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCAcc                                                                    >  1:3571910/1‑150 (MQ=255)
                                                                               aaaaaTGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCAcc                                                                    >  1:3571727/1‑150 (MQ=255)
                                                                                 aaaTGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTc                                                                  <  1:663786/150‑1 (MQ=255)
                                                                                       ggCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATc                                                            <  1:4061710/150‑1 (MQ=255)
                                                                                        gCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATcc                                                           >  2:3213053/1‑150 (MQ=255)
                                                                                           gTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGtt                                                        <  2:4868762/150‑1 (MQ=255)
                                                                                            tAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGtta                                                       <  2:3767831/150‑1 (MQ=255)
                                                                                            tAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGtta                                                       >  2:3874467/1‑150 (MQ=255)
                                                                                                 agTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTaaa                                                  <  2:653833/150‑1 (MQ=255)
                                                                                                 agTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTaaa                                                  >  2:1368787/1‑150 (MQ=255)
                                                                                                 agTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTaaa                                                  <  2:2930478/150‑1 (MQ=255)
                                                                                                 agTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTaaa                                                  <  2:1575958/150‑1 (MQ=255)
                                                                                                      cAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCgg                                             >  1:4767443/1‑150 (MQ=255)
                                                                                                       aaaGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGa                                            >  2:3375245/1‑150 (MQ=255)
                                                                                                       aaaGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGa                                            >  2:1453744/1‑150 (MQ=255)
                                                                                                       aaaGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGa                                            <  2:3853113/150‑1 (MQ=255)
                                                                                                       aaaGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGa                                            >  2:1755070/1‑150 (MQ=255)
                                                                                                       aaaGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGa                                            >  2:4320235/1‑150 (MQ=255)
                                                                                                       aaaGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGa                                            <  2:3852331/150‑1 (MQ=255)
                                                                                                          gCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAaga                                         >  1:819434/1‑150 (MQ=255)
                                                                                                              aTTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAgagaga                                     >  2:2817933/1‑150 (MQ=255)
                                                                                                                  ggTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTg                                 >  2:3680378/1‑150 (MQ=255)
                                                                                                                      ttCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAAtt                             <  1:90326/150‑1 (MQ=255)
                                                                                                                        cGGGCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAAttgt                           <  2:1641102/150‑1 (MQ=255)
                                                                                                                        cGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAAttgt                           >  1:3951169/1‑150 (MQ=255)
                                                                                                                         gggCACCTTTTTCTGCTACTTGAATTCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGACGAGAGCCGTGAAttgtt                          >  1:2856086/1‑150 (MQ=255)
                                                                                                                         gggCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAAttgtt                          <  2:298182/150‑1 (MQ=255)
                                                                                                                         gggCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAAttgta                          <  1:1033234/150‑2 (MQ=255)
                                                                                                                                 ttttCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTtgatgatga                  <  2:1653669/150‑1 (MQ=255)
                                                                                                                                 ttttCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTtgatgatga                  <  1:2340611/150‑1 (MQ=255)
                                                                                                                                     cTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGagaag              >  2:4855076/1‑150 (MQ=255)
                                                                                                                                        cTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGagaagaag           <  1:4247406/150‑1 (MQ=255)
                                                                                                                                            ttGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTTTATCTAATTACATGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGAAGAATAAat       <  2:1824093/150‑1 (MQ=255)
                                                                                                                                            ttGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGATGAAAAAat       <  1:1153924/150‑1 (MQ=255)
                                                                                                                                            ttGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGAAGAAGAAat       <  2:2962942/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGAAGAAGAAATGATGA  >  CP009273/1087596‑1087890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A