Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,193,912 G→A 34.5% intergenic (‑219/‑239) ymfE ← / → lit e14 prophage; putative inner membrane protein/e14 prophage; cell death peptidase, inhibitor of T4 late gene expression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,193,9120GA34.5% 62.3 / 54.4 55intergenic (‑219/‑239)ymfE/lite14 prophage; putative inner membrane protein/e14 prophage; cell death peptidase, inhibitor of T4 late gene expression
Reads supporting (aligned to +/- strand):  ref base G (15/21);  new base A (13/6);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 8.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 105 total aligned reads displayed.

GTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCAT  >  CP009273/1193765‑1194060
                                                                                                                                                   |                                                                                                                                                    
gttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataatgaa                                                                                                                                                    >  1:4619197/1‑150 (MQ=255)
 ttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataatgaaa                                                                                                                                                   >  2:1160917/1‑150 (MQ=255)
 ttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataatgaaa                                                                                                                                                   <  2:3244752/150‑1 (MQ=255)
 ttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataatgaaa                                                                                                                                                   >  2:1242964/1‑150 (MQ=255)
 ttaCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAaatgaaataatgaaataatgaaa                                                                                                                                                   >  2:1242294/1‑150 (MQ=255)
   aCAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATaatgaaatg                                                                                                                                                 <  1:3958631/150‑1 (MQ=255)
     aaTTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgat                                                                                                                                               >  2:598504/1‑150 (MQ=255)
     aaTTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgat                                                                                                                                               >  2:1153619/1‑150 (MQ=255)
      aTTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAataaaatgatg                                                                                                                                              >  1:4800222/1‑150 (MQ=255)
      aTTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAataaaatgatg                                                                                                                                              >  1:4799654/1‑150 (MQ=255)
        tttttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaa                                                                                                                                            >  2:3608929/1‑150 (MQ=255)
         ttttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaa                                                                                                                                           <  1:1752863/150‑1 (MQ=255)
         ttttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAataaaatgatgaaa                                                                                                                                           <  2:4414864/150‑1 (MQ=255)
          tttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaat                                                                                                                                          <  1:2277549/150‑1 (MQ=255)
             gTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgat                                                                                                                                       >  1:1324624/1‑150 (MQ=255)
               aTCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAataaaatgatgaaatgatga                                                                                                                                     >  2:236610/1‑150 (MQ=255)
                  tGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAataaaatgatgaaatgatgaaat                                                                                                                                  >  1:3279947/1‑150 (MQ=255)
                    ttCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatga                                                                                                                                >  2:1753147/1‑150 (MQ=255)
                    ttCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatga                                                                                                                                >  1:3447898/1‑150 (MQ=255)
                     tCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAataaaatgatgaaatgatgaaatgat                                                                                                                               <  1:831317/150‑1 (MQ=255)
                        ggCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAataaaatgatgaaatgatgaaatgatgaa                                                                                                                            >  1:2680198/1‑150 (MQ=255)
                         gCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaa                                                                                                                           <  1:783622/150‑1 (MQ=255)
                         gCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaa                                                                                                                           <  1:783679/150‑1 (MQ=255)
                           tGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatg                                                                                                                         >  2:1065602/1‑150 (MQ=255)
                            gACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatga                                                                                                                        <  1:2939538/150‑1 (MQ=255)
                                 aGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaa                                                                                                                   <  1:2557120/150‑1 (MQ=255)
                                 aGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaa                                                                                                                   >  1:3985483/1‑150 (MQ=255)
                                          gacagacaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaat                                                                                                          <  1:3871629/150‑1 (MQ=255)
                                            cagacaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAAtgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatga                                                                                                        >  2:2080894/1‑150 (MQ=255)
                                                 aaTATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACa                                                                                                   >  1:4001102/1‑150 (MQ=255)
                                                 aaTATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACa                                                                                                   >  1:4394467/1‑150 (MQ=255)
                                                 aaTATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACa                                                                                                   >  2:3133457/1‑150 (MQ=255)
                                                 aaTATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACa                                                                                                   >  2:508031/1‑150 (MQ=255)
                                                 aaTATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATAAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACa                                                                                                   >  2:4093899/1‑150 (MQ=255)
                                                   tatTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATAAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACaga                                                                                                 >  1:4517292/1‑150 (MQ=255)
                                                       gtgATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAgtgt                                                                                             >  1:3309995/1‑150 (MQ=255)
                                                         gATCAGTAGCACTTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGTGTTAGAATATGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTcc                                                                                           <  2:3405013/150‑1 (MQ=255)
                                                         gATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTcc                                                                                           <  2:3405024/150‑1 (MQ=255)
                                                                   aCGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACg                                                                                 <  2:3012212/150‑1 (MQ=255)
                                                                      tATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGAt                                                                              >  2:3698526/1‑150 (MQ=255)
                                                                       aTCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATg                                                                             <  1:1517588/150‑1 (MQ=255)
                                                                       aTCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATg                                                                             >  1:1614560/1‑150 (MQ=255)
                                                                       aTCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATAAAATGATGAAATGATGAAATGATGAAATGATGATATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATg                                                                             <  2:3370052/150‑1 (MQ=255)
                                                                         cGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGgt                                                                           >  2:182741/1‑150 (MQ=255)
                                                                            ggaggagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTc                                                                        >  2:2673196/1‑150 (MQ=255)
                                                                             gaggagTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATAAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCt                                                                       <  2:2214384/150‑1 (MQ=255)
                                                                             gaggagTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATAAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCt                                                                       <  2:200983/150‑1 (MQ=255)
                                                                              aggagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCtt                                                                      >  2:4208989/1‑150 (MQ=255)
                                                                              aggagTAGCGCTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCtt                                                                      >  2:4209015/1‑150 (MQ=255)
                                                                                     gcgcTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATAAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACAt                                                               >  1:4809823/1‑150 (MQ=255)
                                                                                       gcTACAAATTTGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGc                                                             <  1:826217/150‑1 (MQ=255)
                                                                                               tttGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTa                                                     <  2:1520918/150‑1 (MQ=255)
                                                                                                ttGACGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTaa                                                    <  1:377976/150‑1 (MQ=255)
                                                                                                   aCGCTGGGTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCg                                                 <  1:1079333/150‑1 (MQ=255)
                                                                                                         ggTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCa                                           >  1:809877/1‑150 (MQ=255)
                                                                                                         ggTTAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCa                                           <  1:4443794/150‑1 (MQ=255)
                                                                                                          gttagaATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAg                                          <  1:25023/147‑1 (MQ=255)
                                                                                                          gttagaATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAg                                          <  1:25037/147‑1 (MQ=255)
                                                                                                          gttagaATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAg                                          <  1:25485/147‑1 (MQ=255)
                                                                                                          gttagaATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAAAGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAg                                          <  1:24922/147‑1 (MQ=255)
                                                                                                            tagaATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAgtg                                        >  2:772033/2‑150 (MQ=255)
                                                                                                                   tGAAATTGATAGAAATGAAATAATTAAATAATAAAATGATTAAATGATGAAATGATGAAATGATGAAATTAAGAAAATATGACAGATTGTCCAGTGGGCAAGGATGGTGTCTTACGACAAGCTTACCTTAATCGTTCCCAGtgtgaataa                                 >  2:2717367/1‑145 (MQ=37)
                                                                                                                   tGAAATTGATAGAAATGAAATAATGAAATAATAAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCtat                                 >  2:2718007/1‑150 (MQ=255)
                                                                                                                   tGAAATTGATAGAAATGAAATAATGAAATAATAAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCtat                                 >  2:2719202/1‑150 (MQ=255)
                                                                                                                    gAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCtata                                <  1:4307908/150‑1 (MQ=255)
                                                                                                                    gAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCtata                                <  1:4307940/150‑1 (MQ=255)
                                                                                                                    gAAATTGATAGAAATGAAATAATGAAATAATAAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCtata                                <  2:629836/150‑1 (MQ=255)
                                                                                                                      aaTTGATAGAAATGAAATAATGAAATAATAAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGc                              >  2:2090893/1‑150 (MQ=255)
                                                                                                                      aaTTGATAGAAATGAAATAATGAAATAATAAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGc                              >  2:1585156/1‑150 (MQ=255)
                                                                                                                      aaTTGATAGAAATGAAATAATGAAATAATAAAATGATAAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGAGGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGc                              >  2:935121/1‑150 (MQ=255)
                                                                                                                          gATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCGAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGat                          <  1:1757388/150‑1 (MQ=255)
                                                                                                                          gATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGat                          <  1:4271829/150‑1 (MQ=255)
                                                                                                                           aTAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGata                         >  2:3089804/1‑150 (MQ=255)
                                                                                                                           aTAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGata                         <  2:91209/150‑1 (MQ=255)
                                                                                                                           aTAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGata                         <  2:3298100/150‑1 (MQ=255)
                                                                                                                           aTAGAAATGAAATAATGAAATAATAAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGata                         >  2:1196242/1‑150 (MQ=255)
                                                                                                                             aGAAATGAAATAATGAAATAATAAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATAtt                       >  2:4525521/1‑150 (MQ=255)
                                                                                                                             aGAAATGAAATAATGAAATAATAAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATAtt                       >  2:4525495/1‑150 (MQ=255)
                                                                                                                             aGAAATGAAATAACGAAATAATAAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATAtt                       >  2:4525518/1‑150 (MQ=255)
                                                                                                                               aaatgaaatAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCt                     <  1:1153619/150‑1 (MQ=255)
                                                                                                                               aaatgaaatAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCt                     <  1:598504/150‑1 (MQ=255)
                                                                                                                                aatgaaataatgaaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTa                    <  1:1304783/150‑1 (MQ=255)
                                                                                                                                aatgaaataatgaaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTa                    <  1:1304795/150‑1 (MQ=255)
                                                                                                                                aatgaaataatgaaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTa                    <  1:2306072/150‑1 (MQ=255)
                                                                                                                                    aaataatgaaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACa                <  2:2084444/150‑1 (MQ=255)
                                                                                                                                     aataatgaaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACAt               >  2:3738031/1‑150 (MQ=255)
                                                                                                                                     aataatgaaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACAt               >  1:501366/1‑150 (MQ=255)
                                                                                                                                       taatgaaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGt             >  1:3293310/1‑150 (MQ=255)
                                                                                                                                         atgaaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCg           >  2:1792431/1‑150 (MQ=255)
                                                                                                                                          tgaaataataaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGa          <  2:4218455/150‑1 (MQ=255)
                                                                                                                                            aaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATa        >  1:3861347/1‑150 (MQ=255)
                                                                                                                                            aaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATa        >  1:2358768/1‑150 (MQ=255)
                                                                                                                                            aaataataaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATa        >  1:77370/1‑150 (MQ=255)
                                                                                                                                             aataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATaa       >  2:1769567/1‑150 (MQ=255)
                                                                                                                                             aataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATaa       >  2:1770258/1‑150 (MQ=255)
                                                                                                                                              ataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAAt      <  2:45070/150‑1 (MQ=255)
                                                                                                                                              ataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAAt      <  2:44777/150‑1 (MQ=255)
                                                                                                                                              ataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAAt      <  2:44511/150‑1 (MQ=255)
                                                                                                                                                  tgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgaTGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCAt  <  2:4619197/150‑1 (MQ=255)
                                                                                                                                                  tgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgaTGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCAt  <  2:583592/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCAT  >  CP009273/1193765‑1194060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A