Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,603,440 G→A 64.8% intergenic (‑161/+46) yneE ← / ← uxaB bestrophin family putative inner membrane protein/altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,603,4400GA64.8% 224.6 / 240.6 216intergenic (‑161/+46)yneE/uxaBbestrophin family putative inner membrane protein/altronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (44/32);  new base A (78/62);  total (122/94)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-01

Only 100 of 220 total aligned reads displayed.

CGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCT  >  CP009273/1603293‑1603586
                                                                                                                                                   |                                                                                                                                                  
cGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTaaa                                                                                                                                                  >  1:4252274/1‑150 (MQ=255)
 gTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTaaaa                                                                                                                                                 >  1:3902499/1‑150 (MQ=255)
 gTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGaaa                                                                                                                                                 >  2:3522694/1‑150 (MQ=255)
     aaaaGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAg                                                                                                                                             <  1:3437100/150‑1 (MQ=255)
      aaaGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGa                                                                                                                                            <  2:1294346/150‑1 (MQ=255)
      aaaGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACAGTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGa                                                                                                                                            >  2:3041264/1‑150 (MQ=255)
       aaGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGaa                                                                                                                                           <  1:1206970/150‑1 (MQ=255)
        aGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGaaa                                                                                                                                          <  2:4182853/150‑1 (MQ=255)
        aGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGaaa                                                                                                                                          <  2:649042/150‑1 (MQ=255)
            gCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCg                                                                                                                                      <  2:2185425/150‑1 (MQ=255)
              ccTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGt                                                                                                                                    >  2:2685835/1‑150 (MQ=255)
              ccTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGt                                                                                                                                    <  2:1628901/150‑1 (MQ=255)
               cTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTc                                                                                                                                   >  1:4047884/1‑150 (MQ=255)
                   tAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGaaa                                                                                                                               <  2:4221719/150‑1 (MQ=255)
                    aaaCGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAAc                                                                                                                              >  2:365846/1‑150 (MQ=255)
                     aaCGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAAcc                                                                                                                             >  1:4598180/1‑150 (MQ=255)
                      aCGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCg                                                                                                                            <  1:2953627/150‑1 (MQ=255)
                       cGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCgg                                                                                                                           <  1:2261120/150‑1 (MQ=255)
                            ttACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCa                                                                                                                      <  1:2386504/150‑1 (MQ=255)
                              aCGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGa                                                                                                                    >  2:2702097/1‑150 (MQ=255)
                               cGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGaa                                                                                                                   >  2:3957537/1‑150 (MQ=255)
                                gATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAg                                                                                                                  >  1:2516133/1‑150 (MQ=255)
                                       ttCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGgt                                                                                                           <  1:991991/150‑1 (MQ=255)
                                       ttCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGgt                                                                                                           >  2:298923/1‑150 (MQ=255)
                                         ctcAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGgtgt                                                                                                         <  1:2010454/150‑1 (MQ=255)
                                         ctcAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGgtgt                                                                                                         >  1:3917943/1‑150 (MQ=255)
                                         ctcAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGgtgt                                                                                                         >  1:3321512/1‑150 (MQ=255)
                                            aaGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGttat                                                                                                      >  2:3937865/1‑150 (MQ=255)
                                               aCGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAg                                                                                                   <  2:205914/150‑1 (MQ=255)
                                               aCGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAg                                                                                                   >  2:2008713/1‑150 (MQ=255)
                                               aCGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAg                                                                                                   >  1:4809796/1‑150 (MQ=255)
                                                cGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGc                                                                                                  <  1:1050049/150‑1 (MQ=255)
                                                cGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGc                                                                                                  <  1:1048530/150‑1 (MQ=255)
                                                cGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGc                                                                                                  >  2:4872811/1‑150 (MQ=255)
                                                 gCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGca                                                                                                 >  1:2753568/1‑150 (MQ=255)
                                                   tACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGcaca                                                                                               >  1:1534280/1‑150 (MQ=255)
                                                    aCTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACaa                                                                                              >  2:3855403/1‑150 (MQ=255)
                                                     cTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAAc                                                                                             <  1:1080718/150‑1 (MQ=255)
                                                      ttACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACg                                                                                            <  1:688705/150‑1 (MQ=255)
                                                         cAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGAc                                                                                         <  2:226780/150‑1 (MQ=255)
                                                              tACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACa                                                                                    >  1:4563987/1‑150 (MQ=255)
                                                                ccTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGc                                                                                  >  1:713055/1‑150 (MQ=255)
                                                                ccTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGc                                                                                  >  1:1946113/1‑150 (MQ=255)
                                                                  tACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCtt                                                                                <  2:4096447/150‑1 (MQ=255)
                                                                  tACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCtt                                                                                >  1:2437575/1‑150 (MQ=255)
                                                                  tACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCtt                                                                                >  2:4445395/1‑150 (MQ=255)
                                                                     aaGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTcgc                                                                             >  2:2725162/1‑150 (MQ=255)
                                                                      aGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTcgcg                                                                            >  1:3015104/1‑150 (MQ=255)
                                                                      aGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTcgcg                                                                            <  2:2874166/150‑1 (MQ=255)
                                                                        cTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCa                                                                          <  1:2872621/150‑1 (MQ=255)
                                                                         ttACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCAt                                                                         <  1:66947/150‑1 (MQ=255)
                                                                         ttACAACATGAGCAGGCGCTGCATGAGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGAAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCAt                                                                         <  2:302889/150‑1 (MQ=255)
                                                                          tACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATc                                                                        >  2:4002312/1‑150 (MQ=255)
                                                                          tACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATc                                                                        >  2:4773342/1‑150 (MQ=255)
                                                                           acaacaTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATcc                                                                       <  1:4518000/150‑1 (MQ=255)
                                                                           acaacaTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATcc                                                                       <  1:2826424/150‑1 (MQ=255)
                                                                              acaTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCtt                                                                    <  1:3770988/150‑1 (MQ=255)
                                                                               caTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCttt                                                                   <  2:4648873/150‑1 (MQ=255)
                                                                                 ttAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCttttt                                                                 >  1:3839331/1‑150 (MQ=255)
                                                                                  tAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTATCACAACGGACGTACAGCTTCGCGCATCCCTTTTTc                                                                >  2:4537568/1‑150 (MQ=255)
                                                                                  tAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTc                                                                >  1:3815092/1‑150 (MQ=255)
                                                                                         cgcTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATc                                                         <  2:1757136/150‑1 (MQ=255)
                                                                                             gCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCAt                                                     <  2:840292/150‑1 (MQ=255)
                                                                                             gCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCAt                                                     >  1:312752/1‑150 (MQ=255)
                                                                                              cATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGACTCGCATc                                                    >  2:3282824/1‑150 (MQ=255)
                                                                                                tgtgGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCa                                                  >  1:911002/1‑150 (MQ=255)
                                                                                                  tgGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAgg                                                <  2:1696686/150‑1 (MQ=255)
                                                                                                  tgGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAgg                                                >  2:4120723/1‑150 (MQ=255)
                                                                                                  tgGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAgg                                                >  1:1151985/1‑150 (MQ=255)
                                                                                                     cACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCa                                             >  1:2195865/1‑150 (MQ=255)
                                                                                                      aCCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCAt                                            <  2:4381714/150‑1 (MQ=255)
                                                                                                        cTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTa                                          >  1:2447536/1‑150 (MQ=255)
                                                                                                         tGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAg                                         <  1:2692131/150‑1 (MQ=255)
                                                                                                             aaTGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAAc                                     <  1:1681777/150‑1 (MQ=255)
                                                                                                             aaTGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAAc                                     >  2:2419441/1‑150 (MQ=255)
                                                                                                             aaTGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAAc                                     <  2:701168/150‑1 (MQ=255)
                                                                                                              aTGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAAcc                                    >  1:1513473/1‑150 (MQ=255)
                                                                                                               tGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCt                                   <  1:575317/150‑1 (MQ=255)
                                                                                                               tGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCt                                   <  1:3778886/150‑1 (MQ=255)
                                                                                                                gATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTg                                  <  1:4679746/150‑1 (MQ=255)
                                                                                                                 aTAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGc                                 >  2:1598183/1‑150 (MQ=255)
                                                                                                                    aaGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCg                              >  1:955847/1‑150 (MQ=255)
                                                                                                                     aGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCga                             >  2:3625213/1‑150 (MQ=255)
                                                                                                                        cATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgacca                          <  2:1516142/150‑1 (MQ=255)
                                                                                                                        cATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgacca                          >  1:1831060/1‑150 (MQ=255)
                                                                                                                        cATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgacca                          >  1:3677035/1‑150 (MQ=255)
                                                                                                                          tataGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgaccaga                        >  1:4086014/1‑150 (MQ=255)
                                                                                                                             aGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgaccagacca                     <  2:4570186/150‑1 (MQ=255)
                                                                                                                             aGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgaccagacca                     <  1:838316/150‑1 (MQ=255)
                                                                                                                                  gttgtAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCAc                >  1:4486919/1‑150 (MQ=255)
                                                                                                                                  gttgtAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCAc                <  1:1633440/150‑1 (MQ=255)
                                                                                                                                    tgtAACTAATATGGTAAAATAAGAAACCCGGGCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACtt              <  1:3539375/150‑1 (MQ=255)
                                                                                                                                     gtAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTg             >  2:4470907/1‑150 (MQ=255)
                                                                                                                                      tAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGc            >  2:4375578/1‑150 (MQ=255)
                                                                                                                                      tAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGc            >  2:3590245/1‑150 (MQ=255)
                                                                                                                                      tAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGAGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCAGCTCGACCAGACCAGGCACTTGc            >  2:3588865/1‑150 (MQ=255)
                                                                                                                                       aaCTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCg           >  2:2541315/1‑150 (MQ=255)
                                                                                                                                         cTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTc         <  1:199197/150‑1 (MQ=255)
                                                                                                                                             tatGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGt     >  1:2785962/1‑150 (MQ=255)
                                                                                                                                                ggTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCt  >  2:2348602/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCT  >  CP009273/1603293‑1603586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCT  >  CP009273/1603291‑1603589
                                                                                                                                                     |                                                                                                                                                     
GGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTA                                                                                                                                                       >  SRR19973081.196592/1‑150 (MQ=60)
GGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTA                                                                                                                                                       >  SRR19973081.196585/1‑150 (MQ=60)
 GCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAA                                                                                                                                                      <  SRR19973081.4094010/150‑1 (MQ=60)
  CGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAA                                                                                                                                                     <  SRR19973081.2359244/150‑1 (MQ=60)
  CGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAA                                                                                                                                                     >  SRR19973081.4278237/1‑150 (MQ=60)
  CGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAA                                                                                                                                                     <  SRR19973081.1183116/150‑1 (MQ=60)
   GTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAA                                                                                                                                                    >  SRR19973081.3926463/1‑150 (MQ=60)
       AAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAG                                                                                                                                                <  SRR19973081.3458242/150‑1 (MQ=60)
         AAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAA                                                                                                                                              <  SRR19973081.3910579/150‑1 (MQ=60)
         AAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAA                                                                                                                                              <  SRR19973081.1214752/150‑1 (MQ=60)
              GCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCG                                                                                                                                         <  SRR19973081.3915877/150‑1 (MQ=60)
                 CTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTC                                                                                                                                      >  SRR19973081.4075239/1‑150 (MQ=60)
                 CTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTC                                                                                                                                      >  SRR19973081.4072332/1‑150 (MQ=60)
                       AACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACC                                                                                                                                >  SRR19973081.4626080/1‑150 (MQ=60)
                        ACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCG                                                                                                                               <  SRR19973081.2971843/150‑1 (MQ=60)
                         CGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGG                                                                                                                              <  SRR19973081.2273929/150‑1 (MQ=60)
                         CGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGG                                                                                                                              <  SRR19973081.2275405/150‑1 (MQ=60)
                         CGTGATTACGAGCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCAGGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGG                                                                                                                              <  SRR19973081.1424738/150‑1 (MQ=60)
                              TTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCA                                                                                                                         <  SRR19973081.2402381/150‑1 (MQ=60)
                              TTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCA                                                                                                                         <  SRR19973081.2401250/150‑1 (MQ=60)
                                 CGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAA                                                                                                                      <  SRR19973081.2807214/150‑1 (MQ=60)
                                  GATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAG                                                                                                                     >  SRR19973081.2531812/1‑150 (MQ=60)
                                  GATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAG                                                                                                                     >  SRR19973081.4785637/1‑150 (MQ=60)
                                   ATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGT                                                                                                                    >  SRR19973081.3637604/1‑150 (MQ=60)
                                       CATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACG                                                                                                                >  SRR19973081.4235410/1‑150 (MQ=60)
                                         TTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATGAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGT                                                                                                              <  SRR19973081.998724/150‑1 (MQ=60)
                                           CTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGT                                                                                                            >  SRR19973081.3941720/1‑150 (MQ=60)
                                           CTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGT                                                                                                            >  SRR19973081.3941980/1‑150 (MQ=60)
                                           CTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGT                                                                                                            >  SRR19973081.3341835/1‑150 (MQ=60)
                                           CTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGT                                                                                                            <  SRR19973081.2022277/150‑1 (MQ=60)
                                           CTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGT                                                                                                            <  SRR19973081.2022975/150‑1 (MQ=60)
                                                 ACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAG                                                                                                      >  SRR19973081.4838758/1‑150 (MQ=60)
                                                  CGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGC                                                                                                     <  SRR19973081.1055576/150‑1 (MQ=60)
                                                  CGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGC                                                                                                     <  SRR19973081.1055560/150‑1 (MQ=60)
                                                  CGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGC                                                                                                     <  SRR19973081.1057094/150‑1 (MQ=60)
                                                  CGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGC                                                                                                     <  SRR19973081.1057084/150‑1 (MQ=60)
                                                   GCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCA                                                                                                    >  SRR19973081.2770493/1‑150 (MQ=60)
                                                     TACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAagatc                                                                                                  >  SRR19973081.4581806/1‑145 (MQ=60)
                                                     TACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACA                                                                                                  >  SRR19973081.1544300/1‑150 (MQ=60)
                                                     TACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACA                                                                                                  >  SRR19973081.1544270/1‑150 (MQ=60)
                                                       CTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAAC                                                                                                <  SRR19973081.1087887/150‑1 (MQ=60)
                                                        TTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACG                                                                                               <  SRR19973081.693333/150‑1 (MQ=60)
                                                         TACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGG                                                                                              <  SRR19973081.1961719/150‑1 (MQ=60)
                                                         TACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGG                                                                                              <  SRR19973081.926965/150‑1 (MQ=60)
                                                          ACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGA                                                                                             >  SRR19973081.2927261/1‑150 (MQ=60)
                                                             AATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTAAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGT                                                                                          >  SRR19973081.2763694/1‑150 (MQ=60)
                                                                TACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACA                                                                                       >  SRR19973081.4591663/1‑150 (MQ=60)
                                                                  CCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGC                                                                                     >  SRR19973081.717797/1‑150 (MQ=60)
                                                                  CCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGC                                                                                     >  SRR19973081.1958443/1‑150 (MQ=60)
                                                                  CCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGC                                                                                     >  SRR19973081.2977874/1‑150 (MQ=60)
                                                                    TACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTT                                                                                   >  SRR19973081.7051/1‑150 (MQ=60)
                                                                    TACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTT                                                                                   <  SRR19973081.812701/150‑1 (MQ=60)
                                                                    TACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTT                                                                                   >  SRR19973081.2452570/1‑150 (MQ=60)
                                                                        AGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCG                                                                               >  SRR19973081.3033686/1‑150 (MQ=60)
                                                                          CTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCA                                                                             >  SRR19973081.4761399/1‑150 (MQ=60)
                                                                          CTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCA                                                                             <  SRR19973081.2890205/150‑1 (MQ=60)
                                                                           TTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCAT                                                                            <  SRR19973081.67720/150‑1 (MQ=60)
                                                                            TACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATC                                                                           >  SRR19973081.350501/1‑150 (MQ=60)
                                                                             ACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCC                                                                          <  SRR19973081.4545327/150‑1 (MQ=60)
                                                                             ACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCC                                                                          <  SRR19973081.4545326/150‑1 (MQ=60)
                                                                             ACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCC                                                                          >  SRR19973081.1233890/1‑150 (MQ=60)
                                                                             ACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCC                                                                          <  SRR19973081.2843567/150‑1 (MQ=60)
                                                                               AACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCT                                                                        >  SRR19973081.2645976/1‑150 (MQ=60)
                                                                                ACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTT                                                                       <  SRR19973081.3794126/150‑1 (MQ=60)
                                                                                ACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTT                                                                       <  SRR19973081.3793776/150‑1 (MQ=60)
                                                                                   TTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTT                                                                    >  SRR19973081.3862935/1‑150 (MQ=60)
                                                                                   TTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTT                                                                    <  SRR19973081.3000727/150‑1 (MQ=60)
                                                                                    TAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTC                                                                   >  SRR19973081.3838348/1‑150 (MQ=60)
                                                                                    TAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTC                                                                   >  SRR19973081.3838545/1‑150 (MQ=60)
                                                                                        AGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGA                                                               >  SRR19973081.2783155/1‑150 (MQ=60)
                                                                                          GCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAAT                                                             >  SRR19973081.4443862/1‑150 (MQ=60)
                                                                                          GCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAAT                                                             >  SRR19973081.4443849/1‑150 (MQ=60)
                                                                                           CGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATC                                                            <  SRR19973081.1877097/150‑1 (MQ=60)
                                                                                           CGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATC                                                            <  SRR19973081.1876379/150‑1 (MQ=60)
                                                                                           CGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATC                                                            >  SRR19973081.2315309/1‑150 (MQ=60)
                                                                                               GCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCAT                                                        >  SRR19973081.315012/1‑150 (MQ=60)
                                                                                               GCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCAT                                                        >  SRR19973081.315047/1‑150 (MQ=60)
                                                                                                CATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCTCTACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATC                                                       <  SRR19973081.1600144/150‑1 (MQ=60)
                                                                                                CATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATC                                                       <  SRR19973081.3589037/150‑1 (MQ=60)
                                                                                                  TGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCA                                                     >  SRR19973081.917136/1‑150 (MQ=60)
                                                                                                    TGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGG                                                   >  SRR19973081.3432000/1‑150 (MQ=60)
                                                                                                    TGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGG                                                   >  SRR19973081.1159433/1‑150 (MQ=60)
                                                                                                       CACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA                                                >  SRR19973081.2209833/1‑150 (MQ=60)
                                                                                                       CACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA                                                >  SRR19973081.4674152/1‑150 (MQ=60)
                                                                                                       CACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA                                                <  SRR19973081.4594956/150‑1 (MQ=60)
                                                                                                       CACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA                                                >  SRR19973081.602167/1‑150 (MQ=60)
                                                                                                         CCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATT                                              >  SRR19973081.493531/1‑150 (MQ=60)
                                                                                                          CTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTA                                             >  SRR19973081.2462622/1‑150 (MQ=60)
                                                                                                          CTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTA                                             >  SRR19973081.2462629/1‑150 (MQ=60)
                                                                                                          CTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGATTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTA                                             >  SRR19973081.2461819/1‑150 (MQ=60)
                                                                                                           TGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAG                                            >  SRR19973081.687358/1‑150 (MQ=60)
                                                                                                           TGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAG                                            <  SRR19973081.2708819/150‑1 (MQ=60)
                                                                                                             TTAATTATAAATTCATATAGGTTGTTGTAACTAATATGTTAAAATAAGAAACCCTGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCA                                          <  SRR19973081.985391/150‑1 (MQ=60)
                                                                                                              TAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAA                                         <  SRR19973081.4022757/150‑1 (MQ=60)
                                                                                                               AATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAAC                                        <  SRR19973081.1692781/150‑1 (MQ=60)
                                                                                                               AATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAAC                                        <  SRR19973081.1692586/150‑1 (MQ=60)
                                                                                                                ATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACC                                       >  SRR19973081.1523430/1‑150 (MQ=60)
                                                                                                                 TGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCT                                      <  SRR19973081.579403/150‑1 (MQ=60)
                                                                                                                 TGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCT                                      <  SRR19973081.3802078/150‑1 (MQ=60)
                                                                                                                 TGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCT                                      <  SRR19973081.3801366/150‑1 (MQ=60)
                                                                                                                 TGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCT                                      >  SRR19973081.28786/1‑150 (MQ=60)
                                                                                                                  GATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTG                                     <  SRR19973081.4708143/150‑1 (MQ=60)
                                                                                                                      AAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCG                                 >  SRR19973081.962327/1‑150 (MQ=60)
                                                                                                                       AGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGA                                >  SRR19973081.2740735/1‑150 (MQ=60)
                                                                                                                          CATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCA                             >  SRR19973081.1842976/1‑150 (MQ=60)
                                                                                                                          CATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCA                             >  SRR19973081.1842949/1‑150 (MQ=60)
                                                                                                                          CATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCA                             >  SRR19973081.3699326/1‑150 (MQ=60)
                                                                                                                            TATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGA                           >  SRR19973081.4110803/1‑150 (MQ=60)
                                                                                                                               AGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGTTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCA                        <  SRR19973081.3292086/150‑1 (MQ=60)
                                                                                                                               AGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCA                        <  SRR19973081.843678/150‑1 (MQ=60)
                                                                                                                                   TGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCA                    >  SRR19973081.729872/1‑150 (MQ=60)
                                                                                                                                   TGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCA                    >  SRR19973081.385741/1‑150 (MQ=60)
                                                                                                                                    GTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCAC                   >  SRR19973081.4513955/1‑150 (MQ=60)
                                                                                                                                    GTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCAC                   <  SRR19973081.1644052/150‑1 (MQ=60)
                                                                                                                                      TGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTT                 <  SRR19973081.882122/150‑1 (MQ=60)
                                                                                                                                      TGTAACTAATATGGTAAAATAAGAAACCCGGGCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTT                 <  SRR19973081.3560995/150‑1 (MQ=60)
                                                                                                                                        TAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGC               >  SRR19973081.1126841/1‑150 (MQ=60)
                                                                                                                                           CTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTC            <  SRR19973081.200920/150‑1 (MQ=60)
                                                                                                                                               TATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGT        >  SRR19973081.2802992/1‑150 (MQ=60)
                                                                                                                                               TATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGT        >  SRR19973081.2140734/1‑150 (MQ=60)
                                                                                                                                                     AAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCT  >  SRR19973081.2796432/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCT  >  CP009273/1603291‑1603589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑