Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,605,163 G→A 20.8% intergenic (‑226/+1) uxaB ← / ← yneF altronate oxidoreductase, NAD‑dependent/putative membrane‑bound diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,605,1630GA20.8% 412.5 / 99.0 178intergenic (‑226/+1)uxaB/yneFaltronate oxidoreductase, NAD‑dependent/putative membrane‑bound diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (81/60);  new base A (22/15);  total (103/75)
Fisher's exact test for biased strand distribution p-value = 8.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

CTGTTTTGATCAACTCCTGATGATTAATGAGCAGTTTTATGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTACCGACGCCA  >  CP009273/1605016‑1605311
                                                                                                                                                   |                                                                                                                                                    
cTGTTTTGATCAACTCCTGATGATTAATGAGCAGTTTTATGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTc                                                                                                                                                    >  1:3108651/1‑150 (MQ=255)
   ttttGATCAACTCCTGATGATTAATGAGCAGTTTTATGAGACAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGa                                                                                                                                                 >  1:530013/1‑150 (MQ=255)
         tCAACTCCTGATGATTAATGAGCAGTTTTATGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACtt                                                                                                                                           <  1:2921077/150‑1 (MQ=255)
              tCCTGATGATTAATGAGCAGTTTTATGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCa                                                                                                                                      >  2:4809983/1‑150 (MQ=255)
               ccTGATGATTAATGAGCAGTTTTATGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCAc                                                                                                                                     >  1:2312046/1‑150 (MQ=255)
                cTGATGATTAATGAGCAGTTTTATGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCAcc                                                                                                                                    >  2:706976/1‑150 (MQ=255)
                   atgatTAATGAGCAGTTTTATGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTa                                                                                                                                 <  2:4061938/150‑1 (MQ=255)
                    ttattaattaGCAGTTTTATGAGAAAAGTGTGGCGCGTATAATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTaa                                                                                                                                <  2:4501620/148‑1 (MQ=255)
                           tGAGCAGTTTTATGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAg                                                                                                                         <  2:2268350/150‑1 (MQ=255)
                             aGCAGTTTTATGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTg                                                                                                                       >  2:1531259/1‑150 (MQ=255)
                              gCAGTTTTATGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGc                                                                                                                      >  1:1539327/1‑150 (MQ=255)
                                aGTTTTATGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTg                                                                                                                    >  2:2835951/1‑150 (MQ=255)
                                       tGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGt                                                                                                             <  2:1823236/150‑1 (MQ=255)
                                       tGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGt                                                                                                             >  2:292851/1‑150 (MQ=255)
                                       tGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGt                                                                                                             <  2:1823322/150‑1 (MQ=255)
                                       tGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGt                                                                                                             >  2:844603/1‑150 (MQ=255)
                                        gagaAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGtt                                                                                                            <  1:2422068/150‑1 (MQ=255)
                                         agaAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTg                                                                                                           <  2:1895399/150‑1 (MQ=255)
                                         agaAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCAGAGTGCTGGTACGGTTg                                                                                                           >  2:4470289/1‑150 (MQ=255)
                                          gaAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTgc                                                                                                          <  2:4385439/150‑1 (MQ=255)
                                          gaAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTgc                                                                                                          <  2:3372658/150‑1 (MQ=255)
                                           aaaaGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTgcg                                                                                                         >  2:1652740/1‑150 (MQ=255)
                                           aaaaGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTgcg                                                                                                         <  2:3633723/150‑1 (MQ=255)
                                             aaGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGcc                                                                                                       >  1:704548/1‑150 (MQ=255)
                                             aaGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGcc                                                                                                       >  1:704553/1‑150 (MQ=255)
                                              aGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGccc                                                                                                      >  2:525430/1‑150 (MQ=255)
                                              aGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGccc                                                                                                      <  2:2470248/150‑1 (MQ=255)
                                               gtgtGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCa                                                                                                     <  1:4012450/150‑1 (MQ=255)
                                                 gtgGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCAtc                                                                                                   >  2:4858189/1‑150 (MQ=255)
                                                 gtgGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCAtc                                                                                                   >  1:85513/1‑150 (MQ=255)
                                                    gcgcGGATCATGGTTTCATCGAGGCAAAACGCCCTTTCCTGGATCATAAAGTGGGAGAACACATTGCAGTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCAtcttt                                                                                                <  2:2528214/150‑1 (MQ=255)
                                                    gcgcGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCAtcttt                                                                                                >  1:4857915/1‑150 (MQ=255)
                                                    gcgcGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCAtcttt                                                                                                <  2:98055/150‑1 (MQ=255)
                                                    gcgcGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCAtcttt                                                                                                <  1:38461/150‑1 (MQ=255)
                                                       cgGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCAtctttctt                                                                                             >  2:3487463/1‑150 (MQ=255)
                                                          aTCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGa                                                                                          >  2:2873905/1‑150 (MQ=255)
                                                           tCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAg                                                                                         <  2:4685675/150‑1 (MQ=255)
                                                            cATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGc                                                                                        >  1:364773/1‑150 (MQ=255)
                                                             aTGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGGAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCg                                                                                       <  1:1736843/150‑1 (MQ=255)
                                                               ggTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGAt                                                                                     >  2:4374910/1‑150 (MQ=255)
                                                               ggTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGAt                                                                                     >  2:1066987/1‑150 (MQ=255)
                                                                 tttAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATAc                                                                                   <  2:1186656/150‑1 (MQ=255)
                                                                 tttAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAAATTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATAc                                                                                   >  1:1729404/1‑150 (MQ=255)
                                                                     aTCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGac                                                                               >  2:1569300/1‑150 (MQ=255)
                                                                       cGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGacac                                                                             <  1:49748/150‑1 (MQ=255)
                                                                        gAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACg                                                                            <  2:2556181/150‑1 (MQ=255)
                                                                         aGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCAtctttctt                                                                                             <  2:3141082/132‑1 (MQ=255)
                                                                         aGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGt                                                                           >  1:594534/1‑150 (MQ=255)
                                                                         aGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGt                                                                           >  1:4253808/1‑150 (MQ=255)
                                                                           gAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTAt                                                                         <  2:4233563/150‑1 (MQ=255)
                                                                           gAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTAt                                                                         >  1:1966095/1‑150 (MQ=255)
                                                                           gAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTAt                                                                         <  2:2278810/150‑1 (MQ=255)
                                                                                tcGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCt                                                                    >  2:301698/2‑150 (MQ=255)
                                                                                aCGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCt                                                                    <  2:4189647/150‑1 (MQ=255)
                                                                                  gCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTc                                                                  >  1:3800018/1‑150 (MQ=255)
                                                                                  gCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTc                                                                  <  1:4360767/150‑1 (MQ=255)
                                                                                    cTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGa                                                                <  1:3859238/150‑1 (MQ=255)
                                                                                    cTTTTCCTGGATCATAAAGTGGTAGAACACAGTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGa                                                                <  1:4614886/150‑1 (MQ=255)
                                                                                         ccTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCAGCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATc                                                           >  2:4044372/1‑150 (MQ=255)
                                                                                             gATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAAtt                                                       <  1:4116010/150‑1 (MQ=255)
                                                                                              aTCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAAttt                                                      >  2:2445010/1‑150 (MQ=255)
                                                                                                  tAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATACAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCGGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATttatta                                                  <  2:4130496/150‑1 (MQ=255)
                                                                                                     aGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAg                                               >  1:3704432/1‑150 (MQ=255)
                                                                                                     aGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAg                                               <  2:1748635/150‑1 (MQ=255)
                                                                                                     aGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAg                                               <  2:3458262/150‑1 (MQ=255)
                                                                                                       tGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTc                                             <  1:828351/150‑1 (MQ=255)
                                                                                                         gTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCAt                                           <  2:3841927/150‑1 (MQ=255)
                                                                                                          tAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATc                                          <  2:3543287/150‑1 (MQ=255)
                                                                                                               cacaTTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCa                                     <  1:1066987/150‑1 (MQ=255)
                                                                                                                  aTTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCg                                  >  1:103272/1‑150 (MQ=255)
                                                                                                                  aTTGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCg                                  >  1:501934/1‑150 (MQ=255)
                                                                                                                   ttGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGt                                 >  2:253982/1‑150 (MQ=255)
                                                                                                                   ttGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGt                                 <  1:4086486/150‑1 (MQ=255)
                                                                                                                   ttGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGt                                 >  2:3189973/1‑150 (MQ=255)
                                                                                                                    tGCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGtt                                >  2:4434947/1‑150 (MQ=255)
                                                                                                                     gCATTCAAATCGCGCGTAATGAATAAAGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTa                               >  1:4785732/1‑150 (MQ=255)
                                                                                                                      cATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAg                              >  2:1547685/1‑150 (MQ=255)
                                                                                                                         tCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTa                           <  1:912479/150‑1 (MQ=255)
                                                                                                                          cAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTAc                          >  1:4499619/1‑150 (MQ=255)
                                                                                                                          cAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTAc                          >  1:4499623/1‑150 (MQ=255)
                                                                                                                           aaaTCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCACTTTATTAAAGTCATCGGTCAGCGTTAGGTACg                         >  2:3906913/1‑150 (MQ=255)
                                                                                                                           aaaTCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACg                         >  1:2682188/1‑150 (MQ=255)
                                                                                                                           aaaTCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACg                         >  1:2680777/1‑150 (MQ=255)
                                                                                                                             aTCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCCTCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGAt                       >  2:2797929/1‑150 (MQ=255)
                                                                                                                              tCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATg                      >  1:244495/1‑150 (MQ=255)
                                                                                                                               cgcgcgTAATGAATTAAGATGTCAGACAACTTCCTCCCCGTACCGCATAGTGCTGGTACGGTTGCGCCCATCTGTCTTCGAGCGATACAGACACGTATCGGCTTCGACCAGCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATgc                     <  1:2624124/150‑1 (MQ=255)
                                                                                                                               cgcgcgTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATgc                     <  1:191493/150‑1 (MQ=255)
                                                                                                                                   cgTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGa                 >  1:3290890/1‑150 (MQ=255)
                                                                                                                                   cgTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGa                 >  1:3290370/1‑150 (MQ=255)
                                                                                                                                    gTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGAc                <  1:1863243/150‑1 (MQ=255)
                                                                                                                                     tAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGAcc               >  1:1478277/1‑150 (MQ=255)
                                                                                                                                     tAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGAcc               >  2:2686154/1‑150 (MQ=255)
                                                                                                                                       aTGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCAc             <  1:3205944/150‑1 (MQ=255)
                                                                                                                                         gAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCCTCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTa           >  2:2017723/1‑150 (MQ=255)
                                                                                                                                         gAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTa           >  2:4335705/1‑150 (MQ=255)
                                                                                                                                           aTAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTAcc         <  1:4139800/150‑1 (MQ=255)
                                                                                                                                           aTAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTAcc         >  2:1317956/1‑150 (MQ=255)
                                                                                                                                           aTAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTAcc         <  2:1695723/150‑1 (MQ=255)
                                                                                                                                              aaGATATCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTACCGAc      >  2:600657/1‑150 (MQ=255)
                                                                                                                                               aGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTACCGACg     >  2:2125676/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTGTTTTGATCAACTCCTGATGATTAATGAGCAGTTTTATGAGAAAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTACCGACGCCA  >  CP009273/1605016‑1605311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A