Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F83 I1 R1 393 530.9 9427126 91.1% 8588111 148.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,885,942 C→A L56V (CTC→GTA)  FTN78_chr1931 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,885,9420CA100.0% 825.9 / NA 287L56V (CTC→GTA) FTN78_chr1931hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (145/142);  total (145/142)

Only 100 of 287 total aligned reads displayed.

GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAA  >  CP043523/1885816‑1886083
                                                                                                                              |                                                                                                                                             
tgcttATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAg                                                                                                                          >  1:651969/5‑148 (MQ=255)
   ttATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGATCTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAATAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTct                                                                                                                       <  2:1345125/147‑2 (MQ=255)
    tATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGAAAAGAAAATTCAAGCTTTAACACATCATGAAGTCCTTTATCTTCTCAATCAGTctt                                                                                                                      >  1:3046229/1‑146 (MQ=255)
     aTCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCttt                                                                                                                     <  1:2815751/148‑1 (MQ=255)
     aTCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCttt                                                                                                                     >  1:2189305/1‑148 (MQ=255)
      tCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCtttt                                                                                                                    <  2:3943812/148‑1 (MQ=255)
       cACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTtttt                                                                                                                   <  1:4221969/148‑1 (MQ=255)
        aCGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTc                                                                                                                  >  2:2083794/1‑148 (MQ=255)
         cGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTca                                                                                                                 <  2:3585683/148‑2 (MQ=255)
         cGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTca                                                                                                                 >  2:3327189/1‑147 (MQ=255)
          gATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTACTa                                                                                                                >  1:2984364/1‑148 (MQ=255)
            tAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAAt                                                                                                              <  2:2336945/148‑1 (MQ=255)
            tAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAAt                                                                                                              >  1:1802738/1‑148 (MQ=255)
            tAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAAt                                                                                                              <  1:1880564/148‑1 (MQ=255)
             agagGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATc                                                                                                             >  2:2017968/1‑148 (MQ=255)
                   atgatgAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAgctac                                                                                                       >  1:4301914/1‑144 (MQ=255)
                    tgatgaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAgctaca                                                                                                      >  1:392860/1‑143 (MQ=255)
                      atgaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAgctacaaa                                                                                                    <  2:71406/148‑8 (MQ=255)
                      atgaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACaaa                                                                                                    <  2:4533235/148‑1 (MQ=255)
                        gaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAtc                                                                                                  >  2:4216153/1‑147 (MQ=255)
                         aaaTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAtca                                                                                                 >  1:3272697/1‑146 (MQ=255)
                          aaTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaa                                                                                                <  1:4604175/148‑1 (MQ=255)
                           aTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaa                                                                                               >  1:1473299/1‑148 (MQ=255)
                             ggggcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagt                                                                                             <  2:28633/148‑3 (MQ=255)
                             ggggcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagt                                                                                             <  1:1154676/148‑3 (MQ=255)
                               ggcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagtta                                                                                           >  1:3138421/4‑144 (MQ=255)
                               ggcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGtta                                                                                           >  1:2708388/4‑147 (MQ=255)
                                gcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaa                                                                                          >  2:1395826/3‑143 (MQ=255)
                                 cgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGttaaa                                                                                         >  1:1537414/2‑145 (MQ=255)
                                  gCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAat                                                                                        <  1:1792143/148‑1 (MQ=255)
                                   cATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAata                                                                                       >  1:3983045/1‑148 (MQ=255)
                                   cATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAata                                                                                       >  1:1343381/1‑148 (MQ=255)
                                    aTTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAatat                                                                                      >  2:219536/1‑148 (MQ=255)
                                    aTTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAatat                                                                                      <  1:2743521/148‑1 (MQ=255)
                                    aTTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAatat                                                                                      <  1:807399/148‑1 (MQ=255)
                                     tttGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATc                                                                                     <  1:2575279/148‑1 (MQ=255)
                                      ttGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCa                                                                                    >  2:177750/1‑148 (MQ=255)
                                      ttGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCa                                                                                    <  2:41382/148‑1 (MQ=255)
                                          gACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCaatca                                                                                <  2:4500382/148‑4 (MQ=255)
                                           aCTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCaatcaa                                                                               <  1:2317594/148‑5 (MQ=255)
                                           aCTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCGCAATCAGTCTTTTTCAAATCAGCTACAAATCAAGGTTAAATATCaatcaa                                                                               >  2:4437787/1‑144 (MQ=255)
                                             tGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCaaaa                                                                             <  2:4379269/148‑1 (MQ=255)
                                                        atacaGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTaaa                                                                  >  2:1236001/5‑147 (MQ=255)
                                                           aggctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGa                                                               <  2:2991891/143‑1 (MQ=255)
                                                           aggctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGa                                                               >  1:172259/6‑148 (MQ=255)
                                                            ggctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCGAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGaa                                                              <  2:3617556/144‑1 (MQ=255)
                                                            ggctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGaa                                                              <  2:959565/144‑1 (MQ=255)
                                                             gctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAg                                                             >  2:884261/4‑148 (MQ=255)
                                                              ctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGt                                                            >  2:4072873/3‑148 (MQ=255)
                                                               tcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTc                                                           <  2:839984/147‑1 (MQ=255)
                                                               tcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTc                                                           >  1:3627745/2‑148 (MQ=255)
                                                               tcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTc                                                           >  1:2589944/2‑148 (MQ=255)
                                                                 aaaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTa                                                         >  1:1085223/1‑148 (MQ=255)
                                                                 aaaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTa                                                         <  2:495226/148‑1 (MQ=255)
                                                                 aaaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTa                                                         <  2:2313519/148‑1 (MQ=255)
                                                                 aaaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTa                                                         >  1:1663341/1‑148 (MQ=255)
                                                                  aaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCTAAGGTTAAAATAACTGAAGttttt                                                        >  2:3885093/1‑144 (MQ=2)
                                                                  aaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTAt                                                        <  1:4666855/148‑1 (MQ=255)
                                                                    aTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATgg                                                      <  2:3043774/148‑1 (MQ=255)
                                                                      aCTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGtt                                                    >  1:4380614/1‑147 (MQ=255)
                                                                      aCTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGtt                                                    <  1:4576993/148‑2 (MQ=255)
                                                                      aCTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGtt                                                    >  2:2541024/1‑147 (MQ=255)
                                                                       cTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGttt                                                   >  1:4145462/1‑146 (MQ=255)
                                                                        ttttGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGtttt                                                  >  1:1309051/1‑145 (MQ=255)
                                                                         tttGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGttttg                                                 >  2:1484547/1‑144 (MQ=255)
                                                                         tttGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGttttg                                                 >  2:529625/1‑144 (MQ=255)
                                                                                           gatgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaata                               <  2:261087/145‑1 (MQ=9)
                                                                                           gatgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaata                               >  2:1970911/4‑148 (MQ=9)
                                                                                             tgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaataat                             >  2:1788843/2‑148 (MQ=9)
                                                                                              gataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATc                            >  2:549314/1‑148 (MQ=9)
                                                                                               ataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCa                           <  2:1659376/148‑1 (MQ=9)
                                                                                               ataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCa                           <  2:3423262/148‑1 (MQ=9)
                                                                                               ataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCa                           >  1:1819257/1‑148 (MQ=9)
                                                                                                taagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCaa                          >  1:1430145/1‑148 (MQ=9)
                                                                                                   gaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTa                       <  1:4069269/145‑1 (MQ=9)
                                                                                                   gaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTa                       >  2:3729363/4‑148 (MQ=2)
                                                                                                    aaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTaa                      >  1:4478101/3‑148 (MQ=9)
                                                                                                     aaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAag                     >  2:1057/2‑148 (MQ=9)
                                                                                                     aaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAag                     <  2:1121449/147‑1 (MQ=9)
                                                                                                      aaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAaga                    <  1:3716472/148‑1 (MQ=9)
                                                                                                       aTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAagag                   <  1:1395826/148‑1 (MQ=9)
                                                                                                       aTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAagag                   <  1:3025028/148‑1 (MQ=9)
                                                                                                        ttCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGt                  >  2:655897/1‑148 (MQ=9)
                                                                                                         tCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGtt                 >  2:768102/1‑148 (MQ=9)
                                                                                                           aaGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCGGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTaa               <  2:1819822/148‑1 (MQ=9)
                                                                                                           aaGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTaa               <  1:202096/148‑1 (MQ=9)
                                                                                                           aaGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTaa               >  2:4700676/1‑148 (MQ=9)
                                                                                                            aGCATTACCACACCATGTAGTCCCTTGTCTTCTCCATCAGTCTTTTTCAAATCAGCTCCAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTaaa              >  2:3250012/1‑148 (MQ=9)
                                                                                                             gctttACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAAt             >  1:2265961/4‑148 (MQ=9)
                                                                                                             gctttACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAAt             >  2:3480278/4‑148 (MQ=9)
                                                                                                             gctttACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAAt             <  1:3479348/145‑1 (MQ=9)
                                                                                                                ttACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCaa          <  2:1184647/148‑1 (MQ=9)
                                                                                                                 tACCACATCATGTAGTCCTTTATCTTCGCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATGAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAAACAAGTAAGAGGTAAATCAAc         >  2:4323653/1‑148 (MQ=9)
                                                                                                                  aCCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACg        >  2:145549/1‑148 (MQ=9)
                                                                                                                  aCCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACg        >  2:3830274/1‑148 (MQ=9)
                                                                                                                  aCCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACg        <  2:2665073/148‑1 (MQ=9)
                                                                                                                   ccACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACgg       >  2:930455/1‑148 (MQ=9)
                                                                                                                    cacaTCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGa      <  1:962848/148‑1 (MQ=9)
                                                                                                                    cacaTCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGa      >  2:2874567/1‑148 (MQ=9)
                                                                                                                     acaTCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGAt     <  2:591893/148‑1 (MQ=9)
                                                                                                                              |                                                                                                                                             
GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAA  >  CP043523/1885816‑1886083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTTATTAGGATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATA  >  CP043523/1885807‑1886081
                                                                                                                                       |                                                                                                                                           
agtcatccgtgctTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCT                                                                                                                               >  SRR13214404.262992/14‑150 (MQ=60)
agtcatccgtgctTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCT                                                                                                                               <  SRR13214404.2720863/137‑1 (MQ=60)
              ATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTT                                                                                                                 >  SRR13214404.466000/1‑150 (MQ=60)
              ATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTT                                                                                                                 <  SRR13214404.2833839/150‑1 (MQ=60)
              ATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTCACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTT                                                                                                                 <  SRR13214404.839124/150‑1 (MQ=51)
               TCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTC                                                                                                                <  SRR13214404.4337237/150‑1 (MQ=58)
                   GATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAAT                                                                                                            <  SRR13214404.2691135/150‑1 (MQ=51)
                   GATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAAT                                                                                                            >  SRR13214404.1815130/1‑150 (MQ=51)
                     TAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCA                                                                                                          <  SRR13214404.1893442/150‑1 (MQ=48)
                        AGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCT                                                                                                       <  SRR13214404.140797/150‑1 (MQ=45)
                              GATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAT                                                                                                 <  SRR13214404.555990/150‑1 (MQ=42)
                                  aaagggggcgcatttgacactggacaactcccggaggctcaaaataatcTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAA                                                                                             <  SRR13214404.2802332/101‑1 (MQ=23)
                                  AAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAA                                                                                             >  SRR13214404.1483548/1‑150 (MQ=36)
                                  AAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAA                                                                                             >  SRR13214404.1850991/1‑150 (MQ=36)
                                  AAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAA                                                                                             >  SRR13214404.1370206/1‑150 (MQ=36)
                                  AAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAA                                                                                             >  SRR13214404.3133814/1‑150 (MQ=36)
                                            cgtttgcgactggcgatctcaagcagactCAAAATATTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATC                                                                                   <  SRR13214404.267649/121‑1 (MQ=28)
                                            CATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATC                                                                                   >  SRR13214404.2309674/1‑150 (MQ=31)
                                             ATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCA                                                                                  >  SRR13214404.2932216/1‑150 (MQ=31)
                                             ATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCA                                                                                  >  SRR13214404.4451203/1‑150 (MQ=31)
                                             ATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCA                                                                                  <  SRR13214404.2761202/150‑1 (MQ=31)
                                             ATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCA                                                                                  <  SRR13214404.813066/150‑1 (MQ=31)
                                             ATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCA                                                                                  <  SRR13214404.813715/150‑1 (MQ=31)
                                              TTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAA                                                                                 <  SRR13214404.2592017/150‑1 (MQ=31)
                                                     ctggagaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGAGGATAAGAAAATTCAAGCTTTACCACATCATCTAGTGCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGctacaaatcaaagttaaatatcaatcaaaaa                                                                          >  SRR13214404.3943625/21‑119 (MQ=60)
                                                      tggagaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGA                                                                         <  SRR13214404.1602666/131‑1 (MQ=34)
                                                      tggagaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGA                                                                         >  SRR13214404.1897565/20‑150 (MQ=34)
                                                       ggagaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGAT                                                                        <  SRR13214404.854949/132‑1 (MQ=34)
                                                         agaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAA                                                                      >  SRR13214404.2619346/17‑150 (MQ=33)
                                                          gaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAA                                                                     >  SRR13214404.68685/16‑150 (MQ=33)
                                                          gaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAA                                                                     >  SRR13214404.1306766/16‑150 (MQ=33)
                                                           aactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAAT                                                                    <  SRR13214404.4676339/136‑1 (MQ=34)
                                                            actcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATT                                                                   <  SRR13214404.3005812/137‑1 (MQ=36)
                                                            actcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATT                                                                   <  SRR13214404.1347705/137‑1 (MQ=36)
                                                            actcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATT                                                                   <  SRR13214404.772582/137‑1 (MQ=36)
                                                            actcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATT                                                                   <  SRR13214404.314325/137‑1 (MQ=36)
                                                            actcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATT                                                                   >  SRR13214404.2470066/14‑150 (MQ=36)
                                                             ctcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTA                                                                  >  SRR13214404.3090629/13‑150 (MQ=37)
                                                             ctcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTA                                                                  >  SRR13214404.3089837/13‑150 (MQ=37)
                                                             ctcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTA                                                                  >  SRR13214404.2343004/13‑150 (MQ=37)
                                                             ctcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTA                                                                  <  SRR13214404.4253766/138‑1 (MQ=37)
                                                              tcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAA                                                                 <  SRR13214404.3982827/139‑1 (MQ=38)
                                                                acggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAAT                                                               >  SRR13214404.3320793/10‑150 (MQ=38)
                                                                  ggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGA                                                             >  SRR13214404.173551/8‑150 (MQ=38)
                                                                  ggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGA                                                             <  SRR13214404.464430/143‑1 (MQ=38)
                                                                   GAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAA                                                            >  SRR13214404.1711236/1‑150 (MQ=36)
                                                                    aggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAG                                                           >  SRR13214404.4257529/6‑150 (MQ=39)
                                                                    aggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAG                                                           >  SRR13214404.133840/6‑150 (MQ=39)
                                                                      GCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTC                                                         >  SRR13214404.2606769/1‑150 (MQ=41)
                                                                      GCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTC                                                         >  SRR13214404.3650790/1‑150 (MQ=41)
                                                                        TCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTA                                                       <  SRR13214404.3564777/150‑1 (MQ=44)
                                                                        TCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTA                                                       <  SRR13214404.3788542/150‑1 (MQ=44)
                                                                        TCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTA                                                       >  SRR13214404.4607660/1‑150 (MQ=44)
                                                                        TCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTA                                                       >  SRR13214404.1092920/1‑150 (MQ=44)
                                                                        TCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTA                                                       >  SRR13214404.1674855/1‑150 (MQ=44)
                                                                         CAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTAT                                                      >  SRR13214404.2089862/1‑150 (MQ=45)
                                                                         CAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTAT                                                      >  SRR13214404.4639224/1‑150 (MQ=45)
                                                                           AAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGG                                                    <  SRR13214404.4695986/150‑1 (MQ=45)
                                                                           AAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGG                                                    >  SRR13214404.2295313/1‑150 (MQ=45)
                                                                            AATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGT                                                   >  SRR13214404.1884403/1‑150 (MQ=45)
                                                                             ATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAatgcagtcaatggcc                                                  >  SRR13214404.4028529/1‑135 (MQ=33)
                                                                             ATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTT                                                  >  SRR13214404.4408080/1‑150 (MQ=44)
                                                                              TACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTtt                                                 >  SRR13214404.1828689/1‑148 (MQ=43)
                                                                              TACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTtt                                                 >  SRR13214404.4171535/1‑148 (MQ=43)
                                                                               ACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTttt                                                >  SRR13214404.1318287/1‑147 (MQ=41)
                                                                               ACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTttt                                                <  SRR13214404.4605558/150‑4 (MQ=41)
                                                                                CTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTtttg                                               <  SRR13214404.461769/150‑5 (MQ=40)
                                                                                CTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTtttg                                               <  SRR13214404.1441529/150‑5 (MQ=40)
                                                                                CTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTtttg                                               >  SRR13214404.1537117/1‑146 (MQ=40)
                                                                                                   AGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAA                            <  SRR13214404.4097042/150‑1 (MQ=12)
                                                                                                    GATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAAT                           >  SRR13214404.4584737/1‑150 (MQ=12)
                                                                                                      TGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCA                         <  SRR13214404.3491650/150‑1 (MQ=12)
                                                                                                      TGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCA                         >  SRR13214404.1831760/1‑150 (MQ=12)
                                                                                                       GATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAA                        >  SRR13214404.1440149/1‑150 (MQ=12)
                                                                                                        ATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAG                       >  SRR13214404.4091704/1‑150 (MQ=11)
                                                                                                        ATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAG                       >  SRR13214404.3427999/1‑150 (MQ=11)
                                                                                                        ATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAG                       >  SRR13214404.1378960/1‑150 (MQ=11)
                                                                                                           AGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAA                    >  SRR13214404.4506080/1‑150 (MQ=11)
                                                                                                            GAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAG                   <  SRR13214404.4094922/150‑1 (MQ=12)
                                                                                                             AAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGA                  <  SRR13214404.1290084/150‑1 (MQ=14)
                                                                                                              AAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAG                 >  SRR13214404.182156/1‑150 (MQ=15)
                                                                                                               AATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGT                <  SRR13214404.3740036/150‑1 (MQ=16)
                                                                                                                ATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTT               <  SRR13214404.3044395/150‑1 (MQ=16)
                                                                                                                ATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTT               <  SRR13214404.1405613/150‑1 (MQ=16)
                                                                                                                 TTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTA              <  SRR13214404.1728860/150‑1 (MQ=16)
                                                                                                                 TTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTA              >  SRR13214404.3060689/1‑150 (MQ=16)
                                                                                                                  TCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAA             <  SRR13214404.4721638/150‑1 (MQ=16)
                                                                                                                    AAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAAT           <  SRR13214404.203593/150‑1 (MQ=16)
                                                                                                                    AAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAAT           >  SRR13214404.2280999/1‑150 (MQ=16)
                                                                                                                     AGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATC          <  SRR13214404.1288322/150‑1 (MQ=18)
                                                                                                                     AGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATC          <  SRR13214404.3242940/150‑1 (MQ=18)
                                                                                                                      GCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCA         <  SRR13214404.3501532/150‑1 (MQ=19)
                                                                                                                       CTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAA        >  SRR13214404.1320393/1‑150 (MQ=20)
                                                                                                                        TTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAAC       <  SRR13214404.4326597/150‑1 (MQ=22)
                                                                                                                        TTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAAC       <  SRR13214404.2756362/150‑1 (MQ=22)
                                                                                                                        TTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAAC       <  SRR13214404.2755181/150‑1 (MQ=22)
                                                                                                                         TTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACG      <  SRR13214404.1752795/150‑1 (MQ=23)
                                                                                                                             CACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATA  <  SRR13214404.969486/150‑1 (MQ=26)
                                                                                                                                       |                                                                                                                                           
GGTTATTAGGATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATA  >  CP043523/1885807‑1886081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑