Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F83 I1 R1 393 530.9 9427126 91.1% 8588111 148.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,885,979 G→C V69L (GTG→CTA)  FTN78_chr1931 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,885,9790GC99.7% 821.9 / ‑4.5 315V69L (GTG→CTA) FTN78_chr1931hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (150/164);  total (151/164)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 315 total aligned reads displayed.

ATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTAAGCAAATCGTAAATATATC  >  CP043523/1885838‑1886121
                                                                                                                                             |                                                                                                                                              
 tgaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAt                                                                                                                                         <  1:258747/148‑1 (MQ=255)
 tgaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAt                                                                                                                                         <  1:201007/148‑1 (MQ=255)
  gaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAtc                                                                                                                                        >  2:4215874/1‑147 (MQ=255)
    aaTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAtcaa                                                                                                                                      >  2:3855520/1‑145 (MQ=255)
    aaTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaa                                                                                                                                      <  1:4604175/148‑1 (MQ=255)
     aTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaa                                                                                                                                     >  1:1473299/1‑148 (MQ=255)
       ggggcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagt                                                                                                                                   <  1:1154676/148‑3 (MQ=255)
        gggcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGtt                                                                                                                                  >  2:858818/5‑148 (MQ=255)
         ggcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagtta                                                                                                                                 >  1:3138421/4‑144 (MQ=255)
          gcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaa                                                                                                                                <  2:2057677/146‑6 (MQ=255)
          gcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaa                                                                                                                                <  1:1433797/146‑6 (MQ=255)
           cgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGttaaa                                                                                                                               >  1:1537247/2‑145 (MQ=255)
            gCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaaat                                                                                                                              <  1:1792066/148‑8 (MQ=255)
            gCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaaat                                                                                                                              <  1:2780743/148‑8 (MQ=255)
             cATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAata                                                                                                                             <  2:4346069/148‑1 (MQ=255)
             cATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAata                                                                                                                             <  2:4394197/148‑1 (MQ=255)
              aTTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAatat                                                                                                                            <  1:807399/148‑1 (MQ=255)
              aTTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAatat                                                                                                                            >  2:219536/1‑148 (MQ=255)
               tttGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATc                                                                                                                           >  1:2294473/1‑148 (MQ=255)
               tttGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTACTAATCAAGTACAAGTCAAAATTAAATATc                                                                                                                           >  1:4425459/1‑148 (MQ=9)
                 tGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCaa                                                                                                                         >  2:1507841/1‑148 (MQ=255)
                 tGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCaa                                                                                                                         <  1:411461/148‑1 (MQ=255)
                     aCTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCaatcaa                                                                                                                     <  2:1788091/148‑5 (MQ=255)
                     aCTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCaatcaa                                                                                                                     <  1:2317594/148‑5 (MQ=255)
                                  cggaggCCCAAAATACTTTTGAAAAAAAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTaaa                                                                                                        <  2:344729/143‑2 (MQ=255)
                                    gaggctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATg                                                                                                      <  2:950458/142‑1 (MQ=255)
                                     aggctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGa                                                                                                     >  1:172259/6‑148 (MQ=255)
                                       gctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAg                                                                                                   >  1:4230960/4‑148 (MQ=255)
                                       gctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAg                                                                                                   >  2:885035/4‑148 (MQ=255)
                                         tcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTc                                                                                                 >  1:3627745/2‑148 (MQ=255)
                                         tcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTc                                                                                                 <  1:3764657/147‑1 (MQ=255)
                                           aaaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTa                                                                                               <  2:2313519/148‑1 (MQ=255)
                                           aaaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTa                                                                                               >  1:1663341/1‑148 (MQ=255)
                                            aaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTAt                                                                                              <  2:489229/148‑1 (MQ=255)
                                            aaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTAt                                                                                              <  2:2864727/148‑1 (MQ=255)
                                            aaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTAt                                                                                              >  1:2075871/1‑148 (MQ=255)
                                             aaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATg                                                                                             >  2:140472/1‑148 (MQ=255)
                                             aaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATg                                                                                             >  2:1190749/1‑148 (MQ=255)
                                              aTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATgg                                                                                            <  2:3043774/148‑1 (MQ=255)
                                               tACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGt                                                                                           >  1:1871600/1‑148 (MQ=255)
                                                aCTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGCAGTCAATggcc                                                                                          >  1:4003264/1‑146 (MQ=255)
                                                aCTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGtt                                                                                          <  1:4576993/148‑2 (MQ=255)
                                                aCTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGtt                                                                                          >  1:4380614/1‑147 (MQ=255)
                                                 cTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGttt                                                                                         >  2:4565679/1‑146 (MQ=255)
                                                  ttttGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGtttt                                                                                        >  1:1309051/1‑145 (MQ=255)
                                                    ttGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGttttgt                                                                                      >  2:1032865/1‑143 (MQ=255)
                                                                    agatgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaat                                                                      <  2:4069209/144‑1 (MQ=9)
                                                                     gatgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaata                                                                     >  2:1970911/4‑148 (MQ=9)
                                                                      atgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaataa                                                                    >  1:659573/3‑148 (MQ=9)
                                                                       tgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaataat                                                                   >  2:960757/2‑148 (MQ=9)
                                                                        gataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATc                                                                  >  2:549314/1‑148 (MQ=9)
                                                                         ataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCa                                                                 <  2:1659376/148‑1 (MQ=9)
                                                                          taagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCaa                                                                >  2:3217202/1‑148 (MQ=9)
                                                                          taagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCaa                                                                <  2:822249/148‑1 (MQ=9)
                                                                           aagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAg                                                               >  1:3406280/1‑148 (MQ=9)
                                                                            agaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGt                                                              >  2:4472658/5‑148 (MQ=2)
                                                                             gaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTa                                                             >  2:3729363/4‑148 (MQ=2)
                                                                              aaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTaa                                                            <  2:4285257/146‑1 (MQ=9)
                                                                               aaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAag                                                           <  2:1121448/147‑1 (MQ=9)
                                                                                 aTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAagag                                                         >  2:365547/1‑148 (MQ=9)
                                                                                 aTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAagag                                                         >  1:180807/1‑148 (MQ=9)
                                                                                 aTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAagag                                                         <  1:1395826/148‑1 (MQ=9)
                                                                                   tCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGtt                                                       >  2:1302160/1‑148 (MQ=9)
                                                                                    cAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTa                                                      >  1:3041225/1‑148 (MQ=9)
                                                                                     aaGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCGGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTaa                                                     <  2:1819822/148‑1 (MQ=9)
                                                                                     aaGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTaa                                                     >  2:528906/1‑148 (MQ=9)
                                                                                     aaGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTAAAAACAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTAGCAGAATTTATCAATAATCAAGTAAGAGTTaa                                                     <  1:3947006/148‑1 (MQ=9)
                                                                                       gctttACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAAt                                                   <  2:3594891/145‑1 (MQ=9)
                                                                                         tttACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCa                                                 <  1:2737532/147‑1 (MQ=9)
                                                                                            aCCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACg                                              >  2:145549/1‑148 (MQ=9)
                                                                                            aCCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACg                                              <  2:2665073/148‑1 (MQ=9)
                                                                                               acaTCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGAt                                           >  2:392387/1‑148 (MQ=9)
                                                                                                      gtagtccTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatct                                    <  2:706629/142‑1 (MQ=14)
                                                                                                       tagtccTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatcta                                   <  2:65531/143‑1 (MQ=12)
                                                                                                         gtcctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatc                                 <  2:4700533/141‑1 (MQ=12)
                                                                                                         gtccTTTATCTTCTCAATCAGTCTTTTTCCAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatc                                 <  2:4161714/145‑1 (MQ=12)
                                                                                                          tcctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGACGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatct                                >  1:941289/7‑148 (MQ=17)
                                                                                                           cctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTAtctc                               <  2:2913883/143‑1 (MQ=12)
                                                                                                           cctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTAtctc                               >  1:102600/6‑148 (MQ=14)
                                                                                                            ctttaTCTTCTCAATCAGTCTTTTTCTTATTTTCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACTGATAAAATCTATCTCa                              >  2:328760/5‑148 (MQ=9)
                                                                                                             tttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCat                             <  1:2111934/145‑1 (MQ=17)
                                                                                                                aTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATc                          <  1:1315989/148‑1 (MQ=17)
                                                                                                                 tcttctCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATcc                         >  2:2439588/1‑148 (MQ=17)
                                                                                                                      tcaatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAGATGAAGTCTATGGTTTTGTATCAGAATTTATCAATTATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttga                    >  2:4529035/6‑146 (MQ=17)
                                                                                                                      tcaatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttga                    >  2:2596956/6‑146 (MQ=18)
                                                                                                                       caatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaa                   <  1:672976/144‑4 (MQ=17)
                                                                                                                         atcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaaca                 >  1:2880260/3‑143 (MQ=18)
                                                                                                                          tcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaacaa                <  2:3521057/147‑7 (MQ=18)
                                                                                                                          tcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaacaa                <  1:2803376/147‑7 (MQ=18)
                                                                                                                            aGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAt              >  2:2849542/1‑148 (MQ=17)
                                                                                                                              tctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAtta            <  2:2573369/145‑3 (MQ=18)
                                                                                                                              tctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAtta            <  1:3517190/145‑3 (MQ=16)
                                                                                                                               ctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCATAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttat           <  2:2389851/146‑4 (MQ=255)
                                                                                                                               ctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttat           <  2:1343381/146‑4 (MQ=16)
                                                                                                                               ctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttat           <  1:3326256/146‑4 (MQ=16)
                                                                                                                                 ttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttataa         <  2:3326301/148‑6 (MQ=16)
                                                                                                                                   ttcaaaTCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAat       >  2:556627/5‑148 (MQ=12)
                                                                                                                                    tcaaaTCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAata      <  1:1084807/145‑1 (MQ=17)
                                                                                                                                      aaaTCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAatatt    <  2:3138421/147‑2 (MQ=12)
                                                                                                                                       aaTCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAatattt   <  1:1354417/148‑3 (MQ=12)
                                                                                                                                             |                                                                                                                                              
ATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTAAGCAAATCGTAAATATATC  >  CP043523/1885838‑1886121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTAAGCAAATCGTAAATATATCAT  >  CP043523/1885831‑1886123
                                                                                                                                                    |                                                                                                                                                
AGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCT                                                                                                                                                 <  SRR13214404.140797/150‑1 (MQ=45)
      GATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAT                                                                                                                                           <  SRR13214404.555990/150‑1 (MQ=42)
          aaagggggcgcatttgacactggacaactcccggaggctcaaaataatcTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAA                                                                                                                                       <  SRR13214404.2802332/101‑1 (MQ=23)
          AAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAA                                                                                                                                       >  SRR13214404.1483548/1‑150 (MQ=36)
          AAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAA                                                                                                                                       >  SRR13214404.1370206/1‑150 (MQ=36)
          AAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAA                                                                                                                                       >  SRR13214404.3133814/1‑150 (MQ=36)
          AAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAA                                                                                                                                       >  SRR13214404.1850991/1‑150 (MQ=36)
                    cgtttgcgactggcgatctcaagcagactCAAAATATTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATC                                                                                                                             <  SRR13214404.267649/121‑1 (MQ=28)
                    CATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATC                                                                                                                             >  SRR13214404.2309674/1‑150 (MQ=31)
                     ATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCA                                                                                                                            <  SRR13214404.813066/150‑1 (MQ=31)
                     ATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCA                                                                                                                            >  SRR13214404.4451203/1‑150 (MQ=31)
                     ATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCA                                                                                                                            >  SRR13214404.2932216/1‑150 (MQ=31)
                     ATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCA                                                                                                                            <  SRR13214404.2761202/150‑1 (MQ=31)
                     ATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCA                                                                                                                            <  SRR13214404.813715/150‑1 (MQ=31)
                      TTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAA                                                                                                                           <  SRR13214404.2592017/150‑1 (MQ=31)
                              tggagaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGA                                                                                                                   >  SRR13214404.1897565/20‑150 (MQ=34)
                              tggagaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGA                                                                                                                   <  SRR13214404.1602666/131‑1 (MQ=34)
                               ggagaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGAT                                                                                                                  <  SRR13214404.854949/132‑1 (MQ=34)
                                 agaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAA                                                                                                                >  SRR13214404.2619346/17‑150 (MQ=33)
                                  gaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAA                                                                                                               >  SRR13214404.1306766/16‑150 (MQ=33)
                                  gaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAA                                                                                                               >  SRR13214404.68685/16‑150 (MQ=33)
                                   aactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAAT                                                                                                              <  SRR13214404.4676339/136‑1 (MQ=34)
                                    actcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATT                                                                                                             <  SRR13214404.3005812/137‑1 (MQ=36)
                                    actcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATT                                                                                                             >  SRR13214404.2470066/14‑150 (MQ=36)
                                    actcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATT                                                                                                             <  SRR13214404.772582/137‑1 (MQ=36)
                                    actcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATT                                                                                                             <  SRR13214404.1347705/137‑1 (MQ=36)
                                    actcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATT                                                                                                             <  SRR13214404.314325/137‑1 (MQ=36)
                                     ctcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTA                                                                                                            >  SRR13214404.2343004/13‑150 (MQ=37)
                                     ctcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTA                                                                                                            >  SRR13214404.3090629/13‑150 (MQ=37)
                                     ctcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTA                                                                                                            >  SRR13214404.3089837/13‑150 (MQ=37)
                                     ctcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTA                                                                                                            <  SRR13214404.4253766/138‑1 (MQ=37)
                                      tcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAA                                                                                                           <  SRR13214404.3982827/139‑1 (MQ=38)
                                        acggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAAT                                                                                                         >  SRR13214404.3320793/10‑150 (MQ=38)
                                          ggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGA                                                                                                       >  SRR13214404.173551/8‑150 (MQ=38)
                                          ggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGA                                                                                                       <  SRR13214404.464430/143‑1 (MQ=38)
                                           GAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAA                                                                                                      >  SRR13214404.1711236/1‑150 (MQ=36)
                                            aggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAG                                                                                                     >  SRR13214404.4257529/6‑150 (MQ=39)
                                            aggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAG                                                                                                     >  SRR13214404.133840/6‑150 (MQ=39)
                                              GCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTC                                                                                                   >  SRR13214404.2606769/1‑150 (MQ=41)
                                              GCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTC                                                                                                   >  SRR13214404.3650790/1‑150 (MQ=41)
                                                TCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTA                                                                                                 <  SRR13214404.3564777/150‑1 (MQ=44)
                                                TCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTA                                                                                                 <  SRR13214404.3788542/150‑1 (MQ=44)
                                                TCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTA                                                                                                 >  SRR13214404.4607660/1‑150 (MQ=44)
                                                TCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTA                                                                                                 >  SRR13214404.1092920/1‑150 (MQ=44)
                                                TCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTA                                                                                                 >  SRR13214404.1674855/1‑150 (MQ=44)
                                                 CAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTAT                                                                                                >  SRR13214404.4639224/1‑150 (MQ=45)
                                                 CAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTAT                                                                                                >  SRR13214404.2089862/1‑150 (MQ=45)
                                                   AAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGG                                                                                              >  SRR13214404.2295313/1‑150 (MQ=45)
                                                   AAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGG                                                                                              <  SRR13214404.4695986/150‑1 (MQ=45)
                                                    AATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGT                                                                                             >  SRR13214404.1884403/1‑150 (MQ=45)
                                                     ATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAatgcagtcaatggcc                                                                                            >  SRR13214404.4028529/1‑135 (MQ=33)
                                                     ATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTT                                                                                            >  SRR13214404.4408080/1‑150 (MQ=44)
                                                      TACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTtt                                                                                           >  SRR13214404.4171535/1‑148 (MQ=43)
                                                      TACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTtt                                                                                           >  SRR13214404.1828689/1‑148 (MQ=43)
                                                       ACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTttt                                                                                          <  SRR13214404.4605558/150‑4 (MQ=41)
                                                       ACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTttt                                                                                          >  SRR13214404.1318287/1‑147 (MQ=41)
                                                        CTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTtttg                                                                                         >  SRR13214404.1537117/1‑146 (MQ=40)
                                                        CTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTtttg                                                                                         <  SRR13214404.1441529/150‑5 (MQ=40)
                                                        CTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTtttg                                                                                         <  SRR13214404.461769/150‑5 (MQ=40)
                                                                           AGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAA                                                                      <  SRR13214404.4097042/150‑1 (MQ=12)
                                                                            GATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAAT                                                                     >  SRR13214404.4584737/1‑150 (MQ=12)
                                                                              TGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCA                                                                   >  SRR13214404.1831760/1‑150 (MQ=12)
                                                                              TGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCA                                                                   <  SRR13214404.3491650/150‑1 (MQ=12)
                                                                               GATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAA                                                                  >  SRR13214404.1440149/1‑150 (MQ=12)
                                                                                ATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAG                                                                 >  SRR13214404.4091704/1‑150 (MQ=11)
                                                                                ATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAG                                                                 >  SRR13214404.1378960/1‑150 (MQ=11)
                                                                                ATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAG                                                                 >  SRR13214404.3427999/1‑150 (MQ=11)
                                                                                   AGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAA                                                              >  SRR13214404.4506080/1‑150 (MQ=11)
                                                                                    GAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAG                                                             <  SRR13214404.4094922/150‑1 (MQ=12)
                                                                                     AAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGA                                                            <  SRR13214404.1290084/150‑1 (MQ=14)
                                                                                      AAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAG                                                           >  SRR13214404.182156/1‑150 (MQ=15)
                                                                                       AATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGT                                                          <  SRR13214404.3740036/150‑1 (MQ=16)
                                                                                        ATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTT                                                         <  SRR13214404.3044395/150‑1 (MQ=16)
                                                                                        ATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTT                                                         <  SRR13214404.1405613/150‑1 (MQ=16)
                                                                                         TTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTA                                                        <  SRR13214404.1728860/150‑1 (MQ=16)
                                                                                         TTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTA                                                        >  SRR13214404.3060689/1‑150 (MQ=16)
                                                                                          TCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAA                                                       <  SRR13214404.4721638/150‑1 (MQ=16)
                                                                                            AAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAAT                                                     <  SRR13214404.203593/150‑1 (MQ=16)
                                                                                            AAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAAT                                                     >  SRR13214404.2280999/1‑150 (MQ=16)
                                                                                             AGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATC                                                    <  SRR13214404.3242940/150‑1 (MQ=18)
                                                                                             AGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATC                                                    <  SRR13214404.1288322/150‑1 (MQ=18)
                                                                                              GCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCA                                                   <  SRR13214404.3501532/150‑1 (MQ=19)
                                                                                               CTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAA                                                  >  SRR13214404.1320393/1‑150 (MQ=20)
                                                                                                TTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAAC                                                 <  SRR13214404.2755181/150‑1 (MQ=22)
                                                                                                TTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAAC                                                 <  SRR13214404.4326597/150‑1 (MQ=22)
                                                                                                TTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAAC                                                 <  SRR13214404.2756362/150‑1 (MQ=22)
                                                                                                 TTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACG                                                <  SRR13214404.1752795/150‑1 (MQ=23)
                                                                                                     CACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATA                                            <  SRR13214404.969486/150‑1 (MQ=26)
                                                                                                                GTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTC                                 >  SRR13214404.103358/1‑150 (MQ=43)
                                                                                                                GTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTC                                 <  SRR13214404.2097836/150‑1 (MQ=43)
                                                                                                                    TTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATAT                             <  SRR13214404.2813340/150‑1 (MQ=50)
                                                                                                                    TTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATAT                             <  SRR13214404.2126149/150‑1 (MQ=50)
                                                                                                                    TTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATAT                             >  SRR13214404.176180/1‑150 (MQ=50)
                                                                                                                     TTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATC                            >  SRR13214404.943894/1‑150 (MQ=52)
                                                                                                                     TTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATC                            <  SRR13214404.1459703/150‑1 (MQ=52)
                                                                                                                      TATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCC                           >  SRR13214404.1937061/1‑150 (MQ=54)
                                                                                                                       ATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCA                          <  SRR13214404.1325226/150‑1 (MQ=55)
                                                                                                                       ATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCA                          <  SRR13214404.1325274/150‑1 (MQ=55)
                                                                                                                        TCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAT                         <  SRR13214404.1478493/150‑1 (MQ=59)
                                                                                                                         CTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATT                        <  SRR13214404.1488092/150‑1 (MQ=60)
                                                                                                                          TTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTG                       >  SRR13214404.3550908/1‑150 (MQ=60)
                                                                                                                                   AGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTgaacaaatta              >  SRR13214404.3218945/1‑140 (MQ=60)
                                                                                                                                   AGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTgaacaaatta              >  SRR13214404.3358615/1‑140 (MQ=60)
                                                                                                                                     TCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTgaacaaattata            <  SRR13214404.3539602/150‑13 (MQ=60)
                                                                                                                                         TTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAATA        <  SRR13214404.2429117/150‑1 (MQ=60)
                                                                                                                                         TTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAATA        <  SRR13214404.4414952/150‑1 (MQ=60)
                                                                                                                                         TTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAATA        >  SRR13214404.384137/1‑150 (MQ=60)
                                                                                                                                         TTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAATA        <  SRR13214404.10001/150‑1 (MQ=60)
                                                                                                                                          TTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAATAT       <  SRR13214404.661680/150‑1 (MQ=60)
                                                                                                                                              AATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTgaacaaattataaatatttcc   <  SRR13214404.1981707/150‑22 (MQ=60)
                                                                                                                                              AATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTgaacaaattataaatatttcc   <  SRR13214404.1363950/150‑22 (MQ=60)
                                                                                                                                               ATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTgaacaaattataaatatttcct  >  SRR13214404.313520/1‑128 (MQ=60)
                                                                                                                                               ATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTgaacaaattataaatatttcct  >  SRR13214404.1058023/1‑128 (MQ=60)
                                                                                                                                                    |                                                                                                                                                
AGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTAAGCAAATCGTAAATATATCAT  >  CP043523/1885831‑1886123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑