Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I1 R2 775 327.3 10718052 97.2% 10417946 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 2,488,932 (C)7→6 pseudogene (525/3595 nt) evgS → acid‑sensing system histidine kinase EvgS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,488,9260C.98.0% 687.2 / 7.2 151pseudogene (519/3595 nt)evgSacid‑sensing system histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base . (71/75);  total (72/79)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAG  >  NZ_CP025268/2488788‑2489058
                                                                                                                                          |                                                                                                                                    
ttCGCCGCCTCTTAATAATGACATTGCTGCACCCCAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcc                                                                                                                                     >  1:3758025/1‑140 (MQ=255)
ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcc                                                                                                                                     >  1:1659393/1‑140 (MQ=255)
ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcc                                                                                                                                     >  1:1658982/1‑140 (MQ=255)
ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcc                                                                                                                                     >  2:873318/1‑140 (MQ=255)
    ccgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTCcccccc                                                                                                                                 >  1:4258504/1‑140 (MQ=255)
    ccgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccc                                                                                                                                 >  1:2876258/1‑140 (MQ=255)
       ccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccccaga                                                                                                                              <  2:2055114/140‑4 (MQ=255)
         tctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACg                                                                                                                           <  1:2896455/140‑1 (MQ=255)
             aataatGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGCCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGt                                                                                                                       <  2:38909/140‑1 (MQ=255)
                  taaCATAGCTTCAACCAAACCATTGATAATAACCTTTCCGACCCTGTTAATTACCCTTCACGACTAAATGCAACCGCTTACCTCACCAAAACTAGTAAATATTGCTCGTGTAGAAAATTA‑ACCCACATACGAGGTAAAtc                                                                                                                  >  2:3761280/3‑140 (MQ=255)
                  tGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATtc                                                                                                                  >  1:3458444/1‑140 (MQ=255)
                  tGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATtc                                                                                                                  >  1:3068191/1‑140 (MQ=255)
                    aCATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGTTAGCAAATTA‑CCCCCCAGACGAGGTAATtcat                                                                                                                >  1:682368/1‑140 (MQ=255)
                       ttGCTGCAACCAAACCATTGATAATTTCCTTTCCGGCGCTGGTAACGACCCTTAACGACTCAATGCGACCGCGTACCTCACCGAAACCAATAAATATTGCTAGGTGAGCAAAGT‑GCCCCCCAGACGAGGTAATTCatcaa                                                                                                             >  2:168727/1‑140 (MQ=255)
                       ttGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCatcaa                                                                                                             >  1:2168804/1‑140 (MQ=255)
                        tgctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCatcaat                                                                                                            >  1:2204998/1‑140 (MQ=255)
                                  aaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCaaa                                                                                                  >  1:3677200/1‑140 (MQ=255)
                                    accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTaa                                                                                                                     >  2:2254491/1‑119 (MQ=255)
                                    accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTaa                                                                                                                     <  1:2254491/119‑1 (MQ=255)
                                    accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAg                                                                                                <  1:1306616/140‑1 (MQ=255)
                                    accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAg                                                                                                >  1:2855151/1‑140 (MQ=255)
                                    accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAg                                                                                                >  1:2312189/1‑140 (MQ=255)
                                      caTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGca                                                                                              <  1:2453821/140‑1 (MQ=255)
                                         tGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaaca                                                                                           >  1:5228293/1‑140 (MQ=255)
                                            tAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCCCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAtt                                                                                        <  2:2610915/140‑1 (MQ=255)
                                             aaTTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTa                                                                                       >  2:3255573/1‑140 (MQ=255)
                                              aTTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt                                                                                      >  1:2800246/1‑140 (MQ=255)
                                                tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtct                                                                                    >  1:2184785/1‑140 (MQ=255)
                                                tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtct                                                                                    >  1:4625438/1‑140 (MQ=255)
                                                     ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtttt                                                                               >  2:392536/1‑140 (MQ=255)
                                                     ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtttt                                                                               >  1:890067/1‑140 (MQ=255)
                                                             cTGGTAACCACCCTTCACGACTCAATGCGCCCGCTTACCTCACCAAAACCAGTACATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCACTCATTTCCAAAAGCAACAATTATCTCTTTTACAAAttt                                                                       >  1:3560754/1‑140 (MQ=255)
                                                                gTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTtata                                                                    >  1:3923699/1‑140 (MQ=255)
                                                                gTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTtata                                                                    <  2:2469946/140‑1 (MQ=255)
                                                                  aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc                                                                  <  1:1513951/140‑1 (MQ=255)
                                                                  aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc                                                                  <  1:5159201/140‑1 (MQ=255)
                                                                   accaccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCa                                                                 <  2:3966963/140‑1 (MQ=255)
                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAt                                                                         <  1:2782227/125‑1 (MQ=255)
                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAt                                                                         >  2:2782227/1‑125 (MQ=255)
                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGGAATGCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTa                                                          >  1:1066465/1‑140 (MQ=255)
                                                                             aCGACTCCATGCGCCCGCTTCCCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAACTTTATATCAGGCATTAGCa                                                       <  2:682368/140‑1 (MQ=255)
                                                                             aCGACTCCATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                       <  1:197383/140‑1 (MQ=255)
                                                                             aCGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGTGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                       <  2:4301579/140‑1 (MQ=255)
                                                                             aCGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                       <  2:3434791/140‑1 (MQ=255)
                                                                             aCGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                       <  2:1659418/140‑1 (MQ=255)
                                                                             aCGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                       <  2:1658982/140‑1 (MQ=255)
                                                                             aCGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                       <  2:3677200/140‑1 (MQ=255)
                                                                               gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATc                                                     <  2:2004376/140‑1 (MQ=255)
                                                                               gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATc                                                     <  2:4803970/140‑1 (MQ=255)
                                                                                 cTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATGTATATCAGGCATTAGCATCCg                                                   >  2:315414/1‑140 (MQ=255)
                                                                                  tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCTGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAGCTCTTTTACAAATTTATATCAGGCATTAGCATCCGt                                                  >  2:4498240/1‑140 (MQ=255)
                                                                                    aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct                                                >  1:3955025/1‑140 (MQ=255)
                                                                                      tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATGATTTCCAAAAGCAACAATGATCTCTTTTACAAATTTATATCATGAAGTCGCATTCGTCTCa                                              >  1:1176886/1‑140 (MQ=255)
                                                                                      tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCa                                              >  2:321720/1‑140 (MQ=255)
                                                                                      tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCa                                              >  1:3393961/1‑140 (MQ=255)
                                                                                         gACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAAGTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt                                           <  2:1485637/140‑1 (MQ=255)
                                                                                         caCCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt                                           <  2:3059959/139‑1 (MQ=255)
                                                                                          aCCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTg                                          >  2:4939461/1‑140 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                       <  2:1025157/126‑1 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                       >  1:1025157/1‑126 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTgg                                         >  2:2688948/1‑140 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTgg                                         >  1:416662/1‑140 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCGTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTgg                                         >  1:1707705/1‑140 (MQ=255)
                                                                                             gCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                       <  1:5031758/140‑1 (MQ=255)
                                                                                             gCTTACCTCACCAAAACCAGTAAATATTGCGCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                       <  1:5031741/140‑1 (MQ=255)
                                                                                              cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGca                                      <  1:4417772/140‑1 (MQ=255)
                                                                                                     cACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt                               <  1:574939/140‑1 (MQ=255)
                                                                                                     cACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt                               <  2:3355506/140‑1 (MQ=255)
                                                                                                     cACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAGTAGCATCCGTCTCAGCTGGGCACAATGAt                               >  2:2992068/1‑140 (MQ=255)
                                                                                                      aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAtt                              >  2:571850/1‑140 (MQ=255)
                                                                                                             ccAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTCTATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAt                       <  2:4217325/140‑1 (MQ=255)
                                                                                                                gTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTgg                    >  2:2223480/1‑140 (MQ=255)
                                                                                                                gTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTgg                    >  1:4401706/1‑140 (MQ=255)
                                                                                                                    atatTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagt                <  1:1025681/140‑1 (MQ=255)
                                                                                                                    atatTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagt                <  2:1213093/140‑1 (MQ=255)
                                                                                                                     tatTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta               <  1:1978034/140‑1 (MQ=255)
                                                                                                                     tatTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAACCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta               <  1:4450435/140‑1 (MQ=255)
                                                                                                                        tGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct                                                >  2:3352531/1‑104 (MQ=255)
                                                                                                                        tGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            <  1:1591052/140‑1 (MQ=255)
                                                                                                                        tGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            >  2:602172/1‑140 (MQ=255)
                                                                                                                        tGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            >  1:246402/1‑140 (MQ=255)
                                                                                                                        tGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            <  1:3731658/140‑1 (MQ=255)
                                                                                                                        tGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            <  1:602172/140‑1 (MQ=255)
                                                                                                                         gCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAcat           >  1:4683521/1‑140 (MQ=255)
                                                                                                                         gCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAcat           >  2:4851875/1‑140 (MQ=255)
                                                                                                                          cTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAGTGATTACTTTATTGGTAGTAAcatc          >  2:3546314/1‑140 (MQ=255)
                                                                                                                          cTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTTGTAGTAAcatc          >  1:3169408/1‑140 (MQ=255)
                                                                                                                          cTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAcatc          >  1:4110695/1‑140 (MQ=255)
                                                                                                                               gTAGCAAATTA‑CCCCCCAGACGAGGTCATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAc     <  2:3878988/140‑1 (MQ=255)
                                                                                                                               gTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAc     >  1:2364505/1‑140 (MQ=255)
                                                                                                                               gTAGCAAATT‑GCCCCCCAGACGAGGTAATTCATCAAGCATTTCCAAAAGCAACAATTATCTCTATTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAc     <  2:5131770/140‑1 (MQ=255)
                                                                                                                                tAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAcc    <  2:503373/140‑1 (MQ=255)
                                                                                                                                tAGCAAATTA‑CCCCCAAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTATTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTAGGCACAATGATTACTTTATTGGTAGTAACATCATTAcc    <  2:1840606/140‑1 (MQ=255)
                                                                                                                                  gCAAATTA‑CCCCCTAGACGAGGTAATTCATCAATCATTTCTAAAAGCAACAATTATCGCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAGTACTTTATTGGTAGTAAAATGATTACcag  >  2:2274435/1‑140 (MQ=255)
                                                                                                                                  gCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACcag  <  1:2556367/140‑1 (MQ=255)
                                                                                                                                  gCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACcag  <  2:2726783/140‑1 (MQ=255)
                                                                                                                                  gCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACcag  <  2:2333635/140‑1 (MQ=255)
                                                                                                                                  gCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACcag  <  2:2333551/140‑1 (MQ=255)
                                                                                                                                  gCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACcag  <  1:520364/140‑1 (MQ=255)
                                                                                                                                   cAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta               <  2:2812768/126‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                    
TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAG  >  NZ_CP025268/2488788‑2489058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCAC  >  NZ_CP025268/2488782‑2489082
                                                                                                                                                      |                                                                                                                                                      
AGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGTGTAGCAAATTACCCCCCA                                                                                                                                                        >  SRR14920189.1844829/1‑151 (MQ=60)
    ACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACG                                                                                                                                                   <  SRR14920189.3431338/151‑1 (MQ=60)
    ACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACG                                                                                                                                                   <  SRR14920189.3431435/151‑1 (MQ=60)
             CCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGCCGAGGTAATTC                                                                                                                                          >  SRR14920189.3075467/1‑151 (MQ=60)
             CCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTC                                                                                                                                          >  SRR14920189.3466669/1‑151 (MQ=60)
               TCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGTTAGCAAATTA‑CCCCCCAGACGAGGTAATTCAT                                                                                                                                        >  SRR14920189.684095/1‑151 (MQ=60)
               TCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCAT                                                                                                                                        <  SRR14920189.2902921/151‑1 (MQ=60)
                  TAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAA                                                                                                                                     >  SRR14920189.2173814/1‑151 (MQ=60)
                   AATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAAT                                                                                                                                    >  SRR14920189.2210099/1‑151 (MQ=60)
                     taagagacagGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCA                                                                                                                                  <  SRR14920189.2259670/141‑1 (MQ=60)
                          ACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCC                                                                                                                             >  SRR14920189.2239699/1‑151 (MQ=60)
                             TTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTTCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAA                                                                                                                          >  SRR14920189.3685802/1‑151 (MQ=60)
                               GCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAG                                                                                                                        >  SRR14920189.2861536/1‑151 (MQ=60)
                               GCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAG                                                                                                                        >  SRR14920189.2317474/1‑151 (MQ=60)
                               GCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAG                                                                                                                        <  SRR14920189.592385/151‑1 (MQ=60)
                                    AACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACA                                                                                                                   >  SRR14920189.5241180/1‑151 (MQ=60)
                                         AACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAT                                                                                                              >  SRR14920189.2806530/1‑151 (MQ=60)
                                          ACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATC                                                                                                             <  SRR14920189.1309565/151‑1 (MQ=60)
                                           CCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCT                                                                                                            >  SRR14920189.2189838/1‑151 (MQ=60)
                                           CCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCT                                                                                                            >  SRR14920189.4636967/1‑151 (MQ=60)
                                            CATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTC                                                                                                           <  SRR14920189.2459376/151‑1 (MQ=60)
                                                GATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTT                                                                                                       >  SRR14920189.892194/1‑151 (MQ=60)
                                                        CCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGCCCGCTTACCTCACCAAAACCAGTACATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCACTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTT                                                                                               >  SRR14920189.3569158/1‑151 (MQ=60)
                                                           TTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATA                                                                                            >  SRR14920189.3933391/1‑151 (MQ=60)
                                                           TTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATA                                                                                            >  SRR14920189.3553430/1‑151 (MQ=60)
                                                                 acagGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGC                                                                                      <  SRR14920189.2788445/147‑1 (MQ=60)
                                                                     GGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTA                                                                                  >  SRR14920189.3749459/1‑151 (MQ=60)
                                                                     GGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTA                                                                                  >  SRR14920189.2503551/1‑151 (MQ=60)
                                                                     GGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTA                                                                                  >  SRR14920189.3362047/1‑151 (MQ=60)
                                                                     GGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGGAATGCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTA                                                                                  >  SRR14920189.1068914/1‑151 (MQ=60)
                                                                        AACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCA                                                                               <  SRR14920189.1517390/151‑1 (MQ=60)
                                                                        AACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCA                                                                               <  SRR14920189.5171989/151‑1 (MQ=60)
                                                                               CTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCT                                                                        >  SRR14920189.3964839/1‑151 (MQ=60)
                                                                               CTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCT                                                                        >  SRR14920189.3964818/1‑151 (MQ=60)
                                                                                 TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATGATTTCCAAAAGCAACAATGATCTCTTTTACAAATTTATATCAtgaagtcgcattcgtctca                                                                      >  SRR14920189.1179557/1‑132 (MQ=60)
                                                                                 TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCA                                                                      >  SRR14920189.3402086/1‑151 (MQ=60)
                                                                                   ACGACTCCATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGC                                                                    <  SRR14920189.197962/151‑1 (MQ=60)
                                                                                   ACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGC                                                                    <  SRR14920189.2589592/151‑1 (MQ=60)
                                                                                   ACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGC                                                                    <  SRR14920189.1203571/151‑1 (MQ=60)
                                                                                   ACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGC                                                                    <  SRR14920189.3775956/151‑1 (MQ=60)
                                                                                      ACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCctg                                                                 >  SRR14920189.1027529/1‑148 (MQ=60)
                                                                                      ACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGG                                                                 >  SRR14920189.2354554/1‑151 (MQ=60)
                                                                                      ACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGG                                                                 >  SRR14920189.417736/1‑151 (MQ=60)
                                                                                      ACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGG                                                                 >  SRR14920189.758139/1‑151 (MQ=60)
                                                                                      ACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCGTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGG                                                                 >  SRR14920189.1711611/1‑151 (MQ=60)
                                                                                          gctcggagatgtgtataagagacagCCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCAC                                                             <  SRR14920189.3360552/126‑1 (MQ=60)
                                                                                                   GCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTAC                                                    <  SRR14920189.5044347/151‑1 (MQ=60)
                                                                                                   GCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTAC                                                    <  SRR14920189.5044368/151‑1 (MQ=60)
                                                                                                   GCTTACCTCACCAAAACCAGTAAATATTGCGCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTAC                                                    <  SRR14920189.5044330/151‑1 (MQ=60)
                                                                                                    CTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACT                                                   <  SRR14920189.4428622/151‑1 (MQ=60)
                                                                                                      TACCTCACCAAAACCAGTAAATATTGCTAGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTT                                                 <  SRR14920189.4183786/151‑1 (MQ=60)
                                                                                                          TCACCAAAACCAGTAAATCTTGCTCGGGTAGCAAATTACCCCCCC‑GACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTG                                             <  SRR14920189.5202373/151‑1 (MQ=60)
                                                                                                           CACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGG                                            >  SRR14920189.4412530/1‑151 (MQ=60)
                                                                                                           CACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGG                                            <  SRR14920189.576410/151‑1 (MQ=60)
                                                                                                           CACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGG                                            >  SRR14920189.5332749/1‑151 (MQ=60)
                                                                                                            ACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGT                                           <  SRR14920189.5019567/151‑1 (MQ=60)
                                                                                                            ACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGT                                           <  SRR14920189.1508958/151‑1 (MQ=60)
                                                                                                               AAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGT                                        <  SRR14920189.3071540/151‑1 (MQ=60)
                                                                                                                   CCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACA                                    <  SRR14920189.2443745/151‑1 (MQ=60)
                                                                                                                   CCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACA                                    >  SRR14920189.247066/1‑151 (MQ=60)
                                                                                                                    CAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACAT                                   >  SRR14920189.4695265/1‑151 (MQ=60)
                                                                                                                     AGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTTGTAGTAACATC                                  >  SRR14920189.3176922/1‑151 (MQ=60)
                                                                                                                     AGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATC                                  >  SRR14920189.4120847/1‑151 (MQ=60)
                                                                                                                     AGTAAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATC                                  >  SRR14920189.2178326/1‑151 (MQ=60)
                                                                                                                        AAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATT                               >  SRR14920189.3574821/1‑151 (MQ=60)
                                                                                                                        AAATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATT                               >  SRR14920189.4463319/1‑151 (MQ=60)
                                                                                                                          ATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAC                             <  SRR14920189.1028053/151‑1 (MQ=60)
                                                                                                                          ATATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAC                             >  SRR14920189.2369885/1‑151 (MQ=60)
                                                                                                                           TATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACC                            <  SRR14920189.4461450/151‑1 (MQ=60)
                                                                                                                           TATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACC                            <  SRR14920189.1031574/151‑1 (MQ=60)
                                                                                                                           TATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACC                            <  SRR14920189.1982589/151‑1 (MQ=60)
                                                                                                                           TATTGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAACCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACC                            <  SRR14920189.4461451/151‑1 (MQ=60)
                                                                                                                              TGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCctg                         >  SRR14920189.2819069/1‑148 (MQ=60)
                                                                                                                              TGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGC                         <  SRR14920189.2897795/151‑1 (MQ=60)
                                                                                                                              TGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGC                         <  SRR14920189.3740363/151‑1 (MQ=60)
                                                                                                                              TGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGC                         <  SRR14920189.603704/151‑1 (MQ=60)
                                                                                                                              TGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGC                         <  SRR14920189.1596318/151‑1 (MQ=60)
                                                                                                                              TGCTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGC                         <  SRR14920189.1594678/151‑1 (MQ=60)
                                                                                                                                CTCGGGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAG                       <  SRR14920189.2004679/151‑1 (MQ=60)
                                                                                                                                    GGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATG                   >  SRR14920189.3949607/1‑151 (MQ=60)
                                                                                                                                    GGTAGCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATG                   >  SRR14920189.1881021/1‑151 (MQ=60)
                                                                                                                                        GCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTT               <  SRR14920189.2562154/151‑1 (MQ=60)
                                                                                                                                        GCAAATTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTT               <  SRR14920189.521681/151‑1 (MQ=60)
                                                                                                                                             TTA‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAGTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGC          >  SRR14920189.2864567/1‑151 (MQ=60)
                                                                                                                                                  CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATT      >  SRR14920189.3230739/1‑151 (MQ=60)
                                                                                                                                                      CAGACGAGGTAATTCATCACTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCAC  <  SRR14920189.2204107/151‑1 (MQ=60)
                                                                                                                                                      CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCAC  <  SRR14920189.780658/151‑1 (MQ=60)
                                                                                                                                                      |                                                                                                                                                      
AGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCAC  >  NZ_CP025268/2488782‑2489082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑